Recovery of surface orientation from diffuse polarization.

When unpolarized light is reflected from a smooth dielectric surface, it becomes partially polarized. This is due to the orientation of dipoles induced in the reflecting medium and applies to both specular and diffuse reflection. This paper is concerned with exploiting polarization by surface reflection, using images of smooth dielectric objects, to recover surface normals and, hence, height. This paper presents the underlying physics of polarization by reflection, starting with the Fresnel equations. These equations are used to interpret images taken with a linear polarizer and digital camera, revealing the shape of the objects. Experimental results are presented that illustrate that the technique is accurate near object limbs, as the theory predicts, with less precise, but still useful, results elsewhere. A detailed analysis of the accuracy of the technique for a variety of materials is presented. A method for estimating refractive indices using a laser and linear polarizer is also given.     

Design and validation of a high-throughput matrix-assisted laser desorption ionization time-of-flight mass spectrometry method for quantification of hepcidin in human plasma

Disorders of iron metabolism affect over a billion people worldwide. The circulating peptide hormone hepcidin, the central regulator of iron distribution in mammals, holds great diagnostic potential for an array of iron-associated disorders, including iron loading (β-thalassemia), iron overload (hereditary hemochromatosis), and iron deficiency diseases. We describe a novel high-throughput matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry assay for quantification of hepcidin in human plasma. This assay involves enrichment using a functionalized MALDI chip, a novel solvent-detergent precipitation buffer, and quantification using a stable isotope labeled internal standard. The linear range of hepcidin in plasma was 1-120 nM, with a low limit of quantification (LOQ) (1 nM), high accuracy (<15% relative error (RE)), and high precision (intraday average 5.52-18.48% coefficient of variation (CV) and interday 9.32-14.83% CV). The assay showed strong correlation with an established hepcidin immunoassay (Spearman; R(2) = 0.839 n = 93 ethylenediaminetetraacetic acid (EDTA) plasma). A collection of normal healthy pediatric samples (range 3.8-32.5 ng/mL; mean 12.9 ng/mL; n = 119) showed significant differences from an adult collection (range 1.8-48.7 ng/mL; mean 16.1 ng/mL; n = 95; P = 0.0096). We discuss these preliminary reference ranges and correlations with additional parameters in light of the utility and limitations of hepcidin measurements as a stand-alone diagnostic and as a tool for therapeutic intervention.     

Multi-breath dry powder inhaler for delivery of cohesive powders in the treatment of bronchiectasis

A series of co-engineered macrolide–mannitol particles were successfully prepared using azithromycin (AZ) as a model drug. The formulation was designed to target local inflammation and bacterial colonization, via the macrolide component, while the mannitol acted as mucolytic and taste-masking agent. The engineered particles were evaluated in terms of their physico-chemical properties and aerosol performance when delivered via a novel high-payload dry powder Orbital^? inhaler device that operates via multiple inhalation manoeuvres. All formulations prepared were of suitable size for inhalation drug delivery and contained a mixture of amorphous AZ with crystalline mannitol. A co-spray dried formulation containing 200?mg of 50:50?w/w AZ: mannitol had 57.6%?±?7.6% delivery efficiency with a fine particle fraction (≤6.8?µm) of the emitted aerosol cloud being 80.4%?±?1.1%, with minimal throat deposition (5.3?±?0.9%). Subsequently, it can be concluded that the use of this device in combination with the co-engineered macrolide–mannitol therapy may provide a means of treating bronchiectasis.     

Testing Einstein's faux formula: fast computers+slow humans=creative brilliance

An experiment was designed for the purpose of testing the proposition that creative decision-making can be greatly improved by making use of the personal computer to solve a set of word anagrams serving as hints to a surprise phrase. The authors hypothesised that the experimental condition of students using various unscramble word jumble websites would significantly outperform the control group of students who attempted to unscramble the words and solve the surprise answer by hand. Results were mixed and the authors conclude that certain types of creative problem solving exercises might benefit more from the innate abilities and talents of the participants rather than the speed and accuracy of the computer.     

Cognitive Correlates of Improvised Behaviour in Disaster Response: the Cases of the Murrah Building and the World Trade Center

While emergency response actions are known to range from conventional to improvised, less is known about the thinking processes that underlie these actions. This paper presents a statistical analysis of cognition and behaviour reported by police personnel who responded to two significant US disasters: the 1995 bombing of the Murrah Building in Oklahoma City and the 2001 attack on the World Trade Center in New York City. The results suggest the prominence of conventional behaviour coupled with cognitive processes closely tied to recognition, and of improvised behaviours that are linked to more explicit reasoning processes. The results underscore the value of exploring cognitive foundations of both conventional and improvised behaviours to enrich understanding of human response to disaster.     

In Vivo Studies of Nanostructure‐Based Photosensitizers for Photodynamic Cancer Therapy

Animal models, particularly rodents, are major translational models for evaluating novel anticancer therapeutics. In this review, different types of nanostructure‐based photosensitizers that have advanced into the in vivo evaluation stage for the photodynamic therapy (PDT) of cancer are described. This article focuses on the in vivo efficacies of the nanostructures as delivery agents and as energy transducers for photosensitizers in animal models. These materials are useful in overcoming solubility issues, lack of tumor specificity, and access to tumors deep in healthy tissue. At the end of this article, the opportunities made possible by these multiplexed nanostructure‐based systems are summarized, as well as the considerable challenges associated with obtaining regulatory approval for such materials. The following questions are also addressed: (1) Is there a pressing demand for more nanoparticle materials? (2) What is the prognosis for regulatory approval of nanoparticles to be used in the clinic?     

Localization of trehalose in partially hydrated DOPC bilayers: insights into cryoprotective mechanisms

Trehalose, a natural disaccharide with bioprotective properties, is widely recognized for its ability to preserve biological membranes during freezing and dehydration events. Despite debate over the molecular mechanisms by which this is achieved, and that different mechanisms imply quite different distributions of trehalose molecules with respect to the bilayer, there are no direct experimental data describing the location of trehalose within lipid bilayer membrane systems during dehydration. Here, we use neutron membrane diffraction to conclusively show that the trehalose distribution in a dioleoylphosphatidylcholine (DOPC) system follows a Gaussian profile centred in the water layer between bilayers. The absence of any preference for localizing near the lipid headgroups of the bilayers indicates that the bioprotective effects of trehalose at physiologically relevant concentrations are the result of non-specific mechanisms that do not rely on direct interactions with the lipid headgroups.