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排序方式: 共有1944条查询结果,搜索用时 31 毫秒
81.
Georg Schitter Andreas J. Steiner Gerit Pototschnig Elisabeth Scheucher Martin Thonhofer Chris A. Tarling Prof. Stephen G. Withers Katrin Fantur Prof. Eduard Paschke Prof. Don J. Mahuran Brigitte A. Rigat Prof. Michael B. Tropak Carina Illaszewicz Prof. Robert Saf Prof. Arnold E. Stütz Prof. Tanja M. Wrodnigg 《Chembiochem : a European journal of chemical biology》2010,11(14):2026-2033
A collection of new reversible glycosidase inhibitors of the iminoalditol type featuring N‐substituents containing perfluorinated regions has been prepared for evaluation of physicochemical, biochemical and diagnostic properties. The vast variety of feasible oligofluoro moieties allows for modular approaches to customised structures according to the intended applications, which are influenced by the fluorine content as well as the distance of the fluorous moiety from the ring nitrogen. The first examples, in particular in the D ‐galacto series, exhibited excellent inhibitory activities. A preliminary screen with two human cell lines showed that, at subinhibitory concentrations, they are powerful pharmacological chaperones enhancing the activities of the catalytically handicapped lysosomal D ‐galactosidase mutants associated with GM1 gangliosidosis and Morquio B disease. 相似文献
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Soft rot which occurred in timber-based flat roofs of houses in Albertslund, a Copenhagen suburb, provoked a major on-site research and testing programme. The main findings are presented here by Knud Prebensen, of consulting engineers, COWI consult, who worked on this collaborative project and co-authored the original reports and this article with Georg Christensen, of the Danish Building Research Institute, and Prof. Vagn Korsgaard, of the Danish Technical Institute. 相似文献
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The temporal trends and influence of age and gender on levels of selected brominated flame retardants (BFRs) in human serum have been assessed by analyzing archived samples from Norway. Serum from 40 to 50 year old men collected at six time periods during 1977 to 1999 and from eight groups of differing age and gender sampled in 1998 were pooled into six and eight samples, respectively. The BFRs were isolated using solid-phase extraction (SPE) and the serum lipids decomposed bytreatmentwith concentrated sulfuric acid directly on the polystyrene-divinylbenzene SPE column, prior to elution of the BFRs. Following diazomethane derivatization, the samples were analyzed by gas chromatography-electron capture mass spectrometry. Eight BFRs were quantified in the serum samples: 2,4,4'-tribromodiphenyl ether (BDE-28), 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), 2,2',4,4',5-pentabromodiphenyl ether (BDE-99), 2,2',4,4',6-pentabromodiphenyl ether (BDE-100), 2,2',4,4',5,5'-hexabromodiphenyl ether (BDE-153), 2,2',4,4',5,6'-hexabromodiphenyl ether (BDE-154), 2,4,6-tribromophenol (TriBP), and tetrabromobisphenol A (TBBP-A). The serum concentrations of all the BFRs, increased during the entire period with the exception of TriBP, and the sum of the six polybrominated diphenyl ethers increased from 0.44 ng/g lipids in 1977 to 3.3 ng/g lipids in 1999. The BFR concentrations in the serum from the different age groups were relatively similar, except for the age group 0-4 years, which had 1.6-3.5 times higher serum concentrations. Women older than 25 years had lower serum concentrations of BFRs compared to the corresponding group of men. No trend related to age or gender, nor time during the period 1977 to 1999 was observed for TriBP. The present study indicates an ongoing increase in human exposure to BFRs, and the current body burden appears to be independent of age, except for infants (0-4 years old), who seem to experience elevated exposure. 相似文献
88.
The invasive green alga, Caulerpa taxifolia, that has spread rapidly after its introduction into the Mediterranean and the North American Pacific, reacts to wounding by transforming its major metabolite caulerpenyne (1). This wound-activated reaction involves the transformation of the bis-enol acetate moiety of 1, releasing reactive 1,4-dialdehydes. The ability to perform this transformation is found also in both the noninvasive Mediterranean C. prolifera and the invasive C. racemosa. Trapping experiments, as well as transformation of the model substrate geranyl acetate, suggest that all three investigated Caulerpa spp. rely on esterases that act upon wounding of the algae by subsequently removing the three acetate residues of caulerpenyne. The resulting reactive 1,4-dialdehyde oxytoxin 2 (9) can be identified by liquid chromatography–mass spectrometry and is unstable in the wounded tissue. Caulerpenyne transformation occurs rapidly, and severe tissue damage caused degradation of more than 50% of the stored caulerpenyne within 1 min in all three algae. Prevention of the enzymatic reaction before extraction, by shock freezing the tissue with liquid nitrogen, was used for the determination of the caulerpenyne content in intact algae. It gives about twofold higher values compared to an established methanol extraction protocol. The speed and mechanism of the wound-activated transformation, as well as the caulerpenyne content in intact tissue of invasive and noninvasive Caulerpa spp., are comparable. Thus, this enzymatic , transformation, despite being fast and efficient, is likely not the key for the success of the investigated invasive species. 相似文献
89.
Tobias Hanning René Schöne Georg Pisinger 《Journal of Mathematical Imaging and Vision》2006,25(1):5-23
In this article we present an approach to the segmentation problem by a piecewise approximation of the given image with continuous functions. Unlike the common approach of Mumford and Shah in our formulation of the problem the number of segments is a parameter, which can be estimated. The problem can be stated as: Compute the optimal segmentation with a fixed number of segments, then reduce the number of segments until the segmentation result fulfills a given suitability. This merging algorithm results in a multi-objective optimization, which is not only resolved by a linear combination of the contradicting error functions. To constrain the problem we use a finite dimensional vector space of functions in our approximation and we restrict the shape of the segments. Our approach results in a multi-objective optimization: On the one hand the number of segments is to be minimized, on the other hand the approximation error should also be kept minimal. The approach is sound theoretically and practically: We show that for L 2-images a Pareto-optimal solution exists and can be computed for the discretization of the image efficiently. 相似文献
90.
Modal Kleene algebras are Kleene algebras enriched by forward and backward box and diamond operators. We formalise the symmetries of these operators as Galois connections, complementarities and dualities. We study their properties in the associated operator algebras and show that the axioms of relation algebra are theorems at the operator level. Modal Kleene algebras provide a unifying semantics for various program calculi and enhance efficient cross-theory reasoning in this class, often in a very concise pointfree style. This claim is supported by novel algebraic soundness and completeness proofs for Hoare logic and by connecting this formalism with an algebraic decision procedure. 相似文献