Tuning rules for a quick start up in Dynamic Matrix Control

This paper pretends to offer design rules for the parameters adjustment of the Dynamic Matrix Control (DMC) to allow an easier starting up. The effect on the time response of each algorithm parameter that can be tuned by the user is studied in an unconstrained system. To this aim, the position of the closed loop poles of the equivalent system is calculated. To simplify the study and to obtain more direct conclusions the number of poles will be limited using a First Order Plus Death Time simplification of the real plant. Design rules proposed in this study are tested in some simulated benchmarks and in a real plant.     

Gene Therapy in HIV‐Infected Cells to Decrease Viral Impact by Using an Alternative Delivery Method

The ability of dendrimer 2G‐[Si{O(CH₂)₂N(Me)₂⁺(CH₂)₂NMe₃⁺(I^?)₂}]₈ (NN16) to transfect a wide range of cell types, as well as the possible biomedical application in direct or indirect inhibition of HIV replication, was investigated. Cells implicated in HIV infection such as primary peripheral blood mononuclear cells (PBMC) and immortalized suspension cells (lymphocytes), primary macrophages and dendritic cells, and immortalized adherent cells (astrocytes and trophoblasts) were analyzed. Dendrimer toxicity was evaluated by mitochondrial activity, cell membrane rupture, release of lactate dehydrogenase, erythrocyte hemolysis, and the effect on global gene expression profiles using whole‐genome human microarrays. Cellular uptake of genetic material was determined using flow cytometry and confocal microscopy. Transfection efficiency and gene knockdown was investigated using dendrimer‐delivered antisense oligonucleotides and small interfering RNA (siRNA). Very little cytotoxicity was detected in a variety of cells relevant to HIV infection and erythrocytes after NN16 dendrimer treatment. Imaging of cellular uptake showed high transfection efficiency of genetic material in all cells tested. Interestingly, NN16 further enhanced the reduction of HIV protein 24 antigen release by antisense oligonucleotides due to improved transfection efficiency. Finally, the dendrimer complexed with siRNA exhibited therapeutic potential by specifically inhibiting cyclooxygenase‐2 gene expression in HIV‐infected nervous system cells. NN16 dendrimers demonstrated the ability to transfect genetic material into a vast array of cells relevant to HIV pathology, combining high efficacy with low toxicity. These results suggest that NN16 dendrimers have the potential to be used as a versatile non‐viral vector for gene therapy against HIV infection.     

Characterization of human skin impedance after electrical treatment for transdermal drug delivery

A measurement technique based on Electrical Impedance Spectroscopy (EIS) aimed at discriminating electrical effects of the electrical treatment from the electrical characteristics of drug delivery in human skin is presented. The technique turns out to be useful as the first and most crucial step in determining the drug delivered into the skin after electrical treatment. After recalling the background of electrical measurements principle and electrical modeling of biological tissues, the proposed measurement procedure is illustrated. Then, experimental tests of in vivo characterization of the procedure are reported, and the obtained results are discussed.     

Exhaust Emissions from an Engine Fueled with Biodiesel from High-Oleic Soybeans

Biodiesel is a fuel comprising mono-alkyl esters of medium to long-chain fatty acids derived from vegetable oils or animal fats. Typically, engines operated on soybean-based biodiesel exhibit higher emissions of oxides of nitrogen (NOx) compared with petroleum diesel. The increase in NOx emissions might be an inherent characteristic of soybean oil’s polyunsaturation, because the level of saturation is known to affect the biodiesel’s cetane number, which can affect NOx. A feedstock that is mostly monounsaturated (i.e. oleate) helps to balance the tradeoff between cold flow and oxidative stability. Genetic modification has produced a soybean event, designated 335-13, with a fatty acid profile high in oleic acid (>85%) and with reduced palmitic acid (<4%). This high-oleic soybean oil was converted to biodiesel and run in a John Deere 4045T 4.5-L four-stroke, four-cylinder, turbocharged direct-injection diesel engine. The exhaust emissions were compared with those from conventional soybean oil biodiesel and commercial No. 2 diesel fuel. There was a significant reduction in NOx emissions (α = 0.05) using the high-oleic soybean biodiesel compared with regular soybean oil biodiesel. No significant differences were found between the regular and high-oleic biodiesel for unburned hydrocarbon and smoke emissions.     

Natural gas—International: China to supply huge natural gas growth

As the projected fastest-growing fossil fuel through 2030, natural gas is viewed as the “bridge” source to a more sustainable energy future. Combusted gas emits 30 percent less carbon dioxide (CO₂) than oil and 45 percent less than coal and has lower levels of nitrogen oxides, sulfur dioxide, and particulate matter.     

Ochratoxin A in raw materials and cooked meat products made from OTA-treated pigs

The aim of this study was to determine ochratoxin A (OTA) concentrations in the raw materials and cooked meat products made from pigs sub-chronically exposed to OTA. The treated animal group (n = 5) was administered with 300 μg OTA/kg of feed for 30 days, whereas the control group (n = 5) was left untreated. OTA concentrations were quantified using immunoassay (ELISA) and high performance liquid chromatography with fluorescence detection (HPLC-FD). OTA concentration was the highest in the kidney, followed by the lungs, liver, blood, spleen, heart, and adipose tissue. As for the final meat products, the highest average OTA concentration was detected in black pudding sausages (14.02 ± 2.75 μg/kg), then in liver sausages (13.77 ± 3.92 μg/kg), while the lowest was found in pâté (9.33 ± 2.66 μg/kg). The results pointed out that a sub-chronic pig exposure leads to the accumulation of OTA in raw materials and consequently in meat products, whose level of contamination is directly dependent on OTA contents in raw materials used for their production.     

Using a MODFLOW grid, generated with GMS, to solve a transport problem with TOUGH2 in complex geological environments: The intertidal deposits of the Venetian Lagoon

The tides of the Venetian Lagoon generally vary between −0.5 and +0.7 m asl. Occasionally, they may reach a maximum of 1.5 m (acqua alta) and a minimum of −0.8 m asl (acqua bassa). Intertidal areas, called “barene,” exist all along the coast of the Lagoon. These areas are characterized by canals that concentrate the flow of water (and the deposition of sands) during the rising and waning of the tides, and that inundate and drain the vegetated areas found between canals (where organic-rich clays are deposited). Therefore, since the area is subject to subsidence, in time, sand dykes (the original canals) become juxtaposed to clayey dykes (the original vegetated areas). In addition, the sands form a continuous hydrogeologic network within the clays, very similar to that of a vascular system that effectively drains the whole “barena” deposits. In order to be effective, measures for monitoring, confining, or remediating the transport of pollutants through this kind of environment must explicitly take into account the geologic complexity. The same complexity must be included in the numerical models that support remediation efforts. At the moment, there appears to be no off-the-shelf graphical interface that is able to manage such complexity for TOUGH2. To attempt to solve this problem we have used a calibrated USGS-MODFLOW model, of the barena of “Passo a Campalto” in the Venetian Lagoon, developed with the GMS graphical interface. The model is made of 42 layers, which, apart from the first layer, are 0.5 m thick; the first layer has the thickness distribution of a dump found on top of the barena deposit at Passo a Campalto. Each layer consists of 100×60 square cells, for a total of 252,000 cells, only about half of which are active. Using a FORTRAN routine, we translate this grid, with all the hydrogeologic boundary conditions, into a TOUGH2 input file, and we provide the additional necessary information for running a TOUGH2 simulation. The results are promising, in that we were able to produce TOUGH2 grids with very complex geology and to run the models with success. For visualization, the results can be imported back into GMS as 3D scatter point sets, or they can be plotted with any adequate plotting software such as MatLab. Developing conceptual and numerical models with an elaborate graphical interface such as GMS effectively allows setting up complex problems while concentrating on their physics.     

Experimental and numerical analysis of heat transfer and airflow on an interactive building facade

The envelope of a building is mainly responsible for its energy demand. Different kinds of double skin facades (DSFs) are nowadays used as a building envelope to reduce the energy demand and improve aesthetical view of buildings. Although DSF are already extensively used, their thermal performance is not well understood. This study presents a decoupling method capable to evaluate thermal performances and analyze fluid phenomena in a DSF. The solar radiation effects were evaluated with an analytical model and computational fluid dynamics (CFD) simulations were used to evaluate complex flow and thermal effect on a commercial DSF. With the decoupling approach to account for the effects of solar radiation and flow, the numerical results obtained by the CFD approach agree well with the experimental data collected on a full scale test room with a ventilated DSF. The method can be used to establish a database to develop a tool for DSF design.