Preparation and Characterization of Hybrid Nanocomposite Coatings by Cationic UV‐Curing and the Sol‐Gel Process of a Vinyl Ether Based System

Organic/inorganic hybrid nanocomposite coatings were prepared through a dual‐cure process involving the cationic photopolymerization of a vinyl ether based system and the condensation of an alkoxysilane inorganic precursor. All formulations produced transparent cured films characterized by high gel contents. An increase in glass transition temperature and an increase in storage modulus above T_g in the rubbery plateau were observed with increasing TEOS content in the photocurable formulation. TEM micrographs showed that the organic and inorganic phases were strictly interconnected with no macroscopic phase separation; the sizes of the silica domains in the polymeric matrix were 3–5 nm.

     

Detecting similarities among distant homologous proteins by comparison of domain flexibilities

Aim of this work is to assess the informativeness of protein dynamics in the detection of similarities among distant homologous proteins. To this end, an approach to perform large-scale comparisons of protein domain flexibilities is proposed. CONCOORD is confirmed as a reliable method for fast conformational sampling. The root mean square fluctuation of alpha carbon positions in the essential dynamics subspace is employed as a measure of local flexibility and a synthetic index of similarity is presented. The dynamics of a large collection of protein domains from ASTRAL/SCOP40 is analyzed and the possibility to identify relationships, at both the family and the superfamily levels, on the basis of the dynamical features is discussed. The obtained picture is in agreement with the SCOP classification, and furthermore suggests the presence of a distinguishable familiar trend in the flexibility profiles. The results support the complementarity of the dynamical and the structural information, suggesting that information from dynamics analysis can arise from functional similarities, often partially hidden by a static comparison. On the basis of this first test, flexibility annotation can be expected to help in automatically detecting functional similarities otherwise unrecoverable.     

Resolution improvement in digital holography by angular and polarization multiplexing

Angular and polarization multiplexing techniques are utilized in both object and reference arms in the digital holographic microscopy system to improve its resolution. The angular multiplexing provides on-axis and off-axis illumination and reference beams with different carrier frequencies. Polarization multiplexing prohibits the occurrence of interference between low and high object spatial frequencies and reference beams. The proposed system does not require special light sources or filtering masks. Experimental results show that the resolution of the synthesized image exceeds the resolution determined by the numerical aperture of the imaging microscope objective.     

Protein modification by acrolein: relevance to pathological conditions and inhibition by aldehyde sequestering agents

Acrolein (ACR) is a toxic and highly reactive α,β-unsaturated aldehyde widely distributed in the environment as a common pollutant and generated endogenously mainly by lipoxidation reactions. Its biological effects are due to its ability to react with the nucleophilic sites of proteins, to form covalently modified biomolecules which are thought to be involved as pathogenic factors in the onset and progression of many pathological conditions such as cardiovascular and neurodegenerative diseases. Functional impairment of structural proteins and enzymes by covalent modification (crosslinking) and triggering of key cell signalling systems are now well-recognized signs of cell and tissue damage induced by reactive carbonyl species (RCS). In this review, we mainly focus on the in vitro and in vivo evidence demonstrating the ability of ACR to covalently modify protein structures, in order to gain a deeper insight into the dysregulation of cellular and metabolic pathways caused by such modifications. In addition, by considering RCS and RCS-modified proteins as drug targets, this survey will provide an overview on the newly developed molecules specifically tested for direct or indirect ACR scavenging, and the more significant studies performed in the last years attesting the efficacy of compounds already recognized as promising aldehyde-sequestering agents.     

童年的积木 哥伦比亚EL PORVENIR幼儿园

哥伦比亚的建筑师Giancarlo Mazzanti在首都波哥大的郊区为当地的孩子们规划了一座全新的幼儿园。项目所在的地区是一个没有被规划过的贫民区,四周铁锈斑斑的建筑和零星分布的房子无不体现着这个地区的荒凉与边缘化。Giancarlo Mazzanti的新建筑像一道曙光划破黑暗的天际,     

Raw milk from vending machines: Effects of boiling,microwave treatment,and refrigeration on microbiological quality

In Italy, the sale of raw milk from vending machines has been allowed since 2004. Boiling treatment before its use is mandatory for the consumer, because the raw milk could be an important source of foodborne pathogens. This study fits into this context with the aim to evaluate the microbiological quality of 30 raw milk samples periodically collected (March 2013 to July 2013) from 3 vending machines located in Molise, a region of southern Italy. Milk samples were stored for 72 h at 4°C and then subjected to different treatments, such as boiling and microwaving, to simulate domestic handling. The results show that all the raw milk samples examined immediately after their collection were affected by high microbial loads, with values very close to or even greater than those acceptable by Italian law. The microbial populations increased during refrigeration, reaching after 72 h values of about 8.0 log cfu/mL for Pseudomonas spp., 6.5 log cfu/mL for yeasts, and up to 4.0 log cfu/mL for Enterobacteriaceae. Boiling treatment, applied after 72 h to refrigerated milk samples, caused complete decontamination, but negatively affected the nutritional quality of the milk, as demonstrated by a drastic reduction of whey proteins. The microwave treatment at 900 W for 75 s produced microbiological decontamination similar to that of boiling, preserving the content in whey proteins of milk. The microbiological characteristics of raw milk observed in this study fully justify the obligation to boil the raw milk from vending machines before consumption. However, this study also showed that domestic boiling causes a drastic reduction in the nutritional value of milk. Microwave treatment could represent a good alternative to boiling, on the condition that the process variables are standardized for safe domestic application.     

Impact of waste cooking oil in biodiesel blends on particle size distributions from a city-car engine

Using biodiesel as a blending component in diesel engine has demonstrated to reduce hydrocarbon and particulate matter emissions. Literature showed that biodiesel type, engine architecture and test conditions deeply affect performance and emission characteristics. Among suitable biodiesel fuels, waste cooking oil (WCO) is considered very attractive due to the reduced environmental impact without sacrificing engine performance.This paper aims at investigating how mixing ratio of biodiesel from WCO and mineral diesel affects the particle size distributions of a current state of art small displacement diesel engine.Experimental tests have been performed on an up-to date light common rail diesel engine. Its complete operative field has been investigated. The results obtained show that the use of biodiesel blends from WCO reduces the total number of particles emitted from the engine with respect to the diesel fuel; the reduction is more evident as the percentage of biodiesel in the blend increases. The number of particles in WCO biodiesel soot with diameter smaller than 10 nm is reduced as compared to diesel fuel; the same trend is observed for diameters larger than 200 nm; comparable particle numbers were obtained in the ultrafine range (Dp < 100 nm).     

Lactoferrin Inhibition of the Complex Formation between ACE2 Receptor and SARS CoV-2 Recognition Binding Domain

The present investigation focuses on the analysis of the interactions among human lactoferrin (LF), SARS-CoV-2 receptor-binding domain (RBD) and human angiotensin-converting enzyme 2 (ACE2) receptor in order to assess possible mutual interactions that could provide a molecular basis of the reported preventative effect of lactoferrin against CoV-2 infection. In particular, kinetic and thermodynamic parameters for the pairwise interactions among the three proteins were measured via two independent techniques, biolayer interferometry and latex nanoparticle-enhanced turbidimetry. The results obtained clearly indicate that LF is able to bind the ACE2 receptor ectodomain with significantly high affinity, whereas no binding to the RBD was observed up to the maximum “physiological” lactoferrin concentration range. Lactoferrin, above 1 µM concentration, thus appears to directly interfere with RBD–ACE2 binding, bringing about a measurable, up to 300-fold increase of the K_D value relative to RBD–ACE2 complex formation.     

Cane Vinasses Contain Bioactive Concentrations of Auxin and Abscisic Acid in Their Composition

Currently, high doses of vinasse are employed for the fertigation of sugarcane with positive results on yield. Usually, this effect is related to the presence of mineral nutrients in its composition as well as to its action on soil properties. Consequently, the concentrations of minerals, organic acids, and other metabolites in vinasse are very well characterized. However, considering that cane vinasses are obtained from the treatment of vegetal tissues, it is also possible that they might contain significant concentrations of phytoregulators that could have a relevant role in their beneficial action on yield. To investigate this hypothesis, we analyzed the main plant hormones in 22 samples of vinasse collected in different production sites of Brazil using HPLC–mass spectrometry. The results show that both ABA and IAA present concentrations in vinasse within the micromolar range, thus being potential active ingredients affecting plant development. In conclusion, the beneficial action of cane vinasses on sugarcane yield might involve, among other factors, the action of IAA and ABA on plant growth.