Time‐domain imaging system in the terahertz range for immovable cultural heritage materials

In the field of cultural heritage science, the use of non‐destructive and contact‐free techniques has increased sharply over the past 10 years. Compared with conventional spectroscopic and imaging techniques such as X‐ray, ultraviolet, infrared, and laser spectroscopy, terahertz time‐domain imaging (THz‐TDI) is an innovative, non‐invasive, and safe technique, which provides good penetration depth (~1 cm) and broad spectral bandwidth (0.1–10 THz). This paper sets out the protocol and methodology for the application of THz‐TDI to immovable cultural heritage, illustrated by a series of case studies. The case studies demonstrate the efficacy of the technique in providing structural and material information for conservators.     

Inward Translocation of the Phospholipid Analogue Miltefosine across Caco-2 Cell Membranes Exhibits Characteristics of a Carrier-mediated Process

Miltefosine (hexadecylphosphocholine, HePC) is the first effective oral agent for the treatment of visceral leishmaniasis. The characteristics of HePC incorporation into the human intestinal epithelial cell line Caco-2 were investigated in order to understand its oral absorption mechanism. The results provide evidence for the involvement of a carrier-mediated mechanism, since the association of HePC at the apical pole of Caco-2 cells was (1) saturable as a function of time with a rapid initial incorporation over 5 min followed by a more gradual increase; (2) saturable as a function of concentration over the range studied (2–200 μM) with a saturable component which followed Michaelis–Menten kinetics (apparent K _m 15.7 μmol/L, V _max 39.2 nmol/mg protein/h) and a nonspecific diffusion component; (3) partially inhibited by low temperature and ATP depletion, indicating the temperature and energy-dependence of the uptake process. Moreover, we demonstrated, by an albumin back-extraction method, that HePC is internalized via translocation from the outer to the inner leaflet of the plasma membrane and that HePC may preferentially diffuse through intact raft microdomains. In conclusion, our results suggest that incorporation of HePC at the apical membrane of Caco-2 cells may occur through a passive diffusion followed by a translocation in the inner membrane leaflet through an active carrier-mediated mechanism.     

Accumulation of current-use pesticides in neotropical montane forests

In Central America, chemical-intensive tropical agriculture takes place in close proximity to highly valued and biologically diverse ecosystems, yet the potential for atmospheric transport of pesticides from plantations to national parks and other reserves is poorly characterized. The specific meteorological conditions of mountain ranges can lead to contaminant convergence at high altitudes, raising particular concern for montane forest ecosystems downwind from pesticide use areas. Here we show, based on a wide-ranging air and soil sampling campaign across Costa Rica, that soils in some neotropical montane forests indeed display much higher concentrations of currently used pesticides than soils elsewhere in the country. Specifically, elevated concentrations of the fungicide chlorothalonil, the herbicide dacthal, and the insecticide metabolite endosulfan sulfate on volcanoes Barva and Poas, lying directly downwind of the extensive banana plantations of the Caribbean lowland, indicate the occurrence of atmospheric transport and wet deposition of pesticides at high altitudes. Calculations with a contaminant fate model, designed for mountain regions and parametrized to the Costa Rican environment, show that chemicals with a log K(AW) between -3 and -5 have a greater potential for accumulation at high altitudes. This enrichment behavior is quantified by the Mountain Contamination Potential and is sensitive to contaminant degradability. The modeling work supports the hypothesis suggested by the field results that it is enhanced precipitation scavenging at high elevations (caused by lower temperatures and governed by K(AW)) that causes pesticides to accumulate in tropical montane areas. By providing for the first time evidence of significant transfer of currently used pesticides to Central American montane cloud forests, this study highlights the need to evaluate the risk that tropical agricultural practices place on the region's ecological reserves.     

Projecting transition probabilities for regular public roads at the ecoregion scale: A Northern Appalachian/Acadian case study

Existing roads have far-reaching effects on biodiversity, and therefore road network expansion is of critical concern to conservation planning. Road density trend analysis is often too coarse and assumes homogeneous landscapes, whereas spatial transition probability analysis captures landscape variability typical of ecoregions. Simple models for projecting road network growth will assist planning agencies and conservation organizations to guide protection efforts. We investigate growth of regular public roads in the State of Maine over a 17-year historical period, and then use the best-selected (AIC) logistic regression model to validate and then project spatial probability of future roads to the Northern Appalachian/Acadian ecoregion. Nearly 2000 km of new roads were constructed in settled landscapes in Maine 1986–2003, influencing 37,000 ha of adjacent habitats. The majority (93.5%) of the new roads performed local functions and were short (<1/3 km in length), characterized as residential roads typical of sprawl. The best-selected logit model [dwelling density (+), elevation (−), distance to urban area (−), distance to existing primary/secondary highway (−)] captured 84% of reserved new road points in Maine, and only 27% of random points at the >0.5 probability level. The projected model forecasts 0.5 million km of new residential public roads in the Northern Appalachian/Acadian ecoregion for the next two decades, suggesting that cumulative effects of residential road network expansion are a serious region-scale biodiversity threat.     

The effect of frozen storage of mackerel (Scomber scombrus) on its quality when hot-smoked

Whole mackerel (Scomber scombrus) were frozen using a horizontal plate freezer, wrapped in plastic bags and frozen stored at - 20°C. At suitable intervals (11, 22 and 33 weeks) the fish were removed from the cold store and subsequently hot smoked (in gutted from) using an AFOS-Torry Mini Kiln. All smoked mackerel samples, despite their different previous histories, were assessed by the panellists as moderately acceptable products in- terms of their texture and flavour, even after 33 weeks frozen storage prior to smoking. Protein denaturation, as related to salt-soluble protein, was influenced by the frozen storage history (24% drop after 33 weeks frozen storage) and seemed to be affected by the free amino acids formed during frozen storage. After smoking the denaturation was extensive (above 80%) in all mackerel samples. Lipid oxidation was quite extensive (PV 108 meq kg^?1) in the 22 and 33 weeks frozen stored mackerel samples. However, no rancid flavour in the latter smoked mackerel samples was detected by taste panellists. A 58% increase in free amino acids during frozen storage was observed. Extensive losses of 74% in available lysine were observed in the 22 and 33 weeks frozen stored mackerel samples after smoking which could be due to aminocarbonyl reactions with the products of lipid oxidation. A 40% loss of thiamine was observed in the 33 weeks frozen stored samples after smoking. The histamine contents did not exceed 94 mg kg^?1 and would not be expected to cause symptoms of scombrotoxin poisoning.     

BikeSafe: Evaluating a bicycle safety program for middle school aged children

Purpose

The purpose of this study is to measure the effectiveness of a bicycle safety education curriculum for middle school age children in order to reduce the number of injuries and fatalities of bicyclists hit by cars in Miami-Dade County.

Methods

The University of Miami BikeSafe^® program includes a four day off-bike middle school curriculum that follows a train-the-trainer model, where a small number of staff trains a larger group of grades 6th–8th physical education teachers from various schools to teach the bike safety curriculum to their students. Subjects in this study included 193 students from 18 classes (3 per school) at 6 selected middle schools. Measures included a knowledge assessment of the curriculum that was administered to students pre- and post-curriculum implementation. Data were collected and analyzed with school and class period examined as predictors of post-score.

Results

A significant difference (p < .001) was found between pre- and post-test conditions across all subjects. In addition, there was no significant difference between testing from class periods (p > .05), suggesting that a standard intervention was applied.

Conclusion

The BikeSafe educational curriculum was found to improve the bike safety knowledge of middle school aged children. Future efforts will focus on sustaining and expanding this program throughout Miami-Dade County and other high risk communities.     

How different is human hair? A critical appraisal of the reported differences in global hair fibre characteristics and properties towards defining a more relevant framework for hair type classification

This review critically appraises the reported differences in human hair fibre within three related domains of research: hair classification approaches, fibre characteristics and properties. The most common hair classification approach is based on geo-racial origin, defining three main groups: African, Asian and Caucasian hair. This classification does not account sufficiently for the worldwide hair diversity and intergroups variability in curl, shape, size and colour. A global classification into eight curl types has been proposed but may be too complex for reproducibility. Beyond that, hair cross-sectional shape and area have been found to have an inverse relation to curl: straighter fibres are circular with larger cross-sectional area, whilst the curlier fibres are elliptical with smaller cross-sectional area. These geometrical differences have been associated with bilateral vs homogenous distribution of cortical cell in curly vs straight hair respectively. However, there is no sufficient data demonstrating significant differences in hair amino composition, but proteomic studies are reporting associations of some proteins with curly hair. Eumelanin’s relative abundance has been reported in all hair colours except for red hair which has a high pheomelanin content. Higher tensile and fatigue strength of straight hair are reported, however, curly hair fragility is attributed to knotting, and crack and flow formations rather than the structural variations. African hair has been found to have the highest level of lipids, whilst the water sorption of Caucasian hair is the highest, and that of Asian hair the lowest. Not all comparative studies clearly report their hair sampling approaches. Therefore, to strengthen the robustness of comparative studies and to facilitate cross-study data comparisons, it is recommended that the following hair defining characteristics are reported in studies: hair cross sectional diameter/area, curl type, hair assembly colour, as well as where possible donor data (age/gender) and sample pooling approach.