Effect of potentially probiotic lactobacilli on faecal enzyme activity in minipigs on a high-fat, high-cholesterol diet-a preliminary in vivo trial

Minipigs were fed a "Western-style", high-cholesterol diet for a baseline period, followed by the diet containing a mixture of three Lactobacillus strains with potential probiotic features, after which a normal pig diet was followed. The faecal enzyme activity for beta-glucuronidase and azoreductase, which are commonly considered as markers for procarcinogenic activity, was significantly reduced during the 5 weeks of "probiotic" supplementation. During the period of Lactobacillus administration, the cell counts for total anaerobes increased, whereas the total number of aerobes showed no change.     

High-gain observers in the presence of measurement noise: A switched-gain approach

This paper considers output feedback control using high-gain observers in the presence of measurement noise for a class of nonlinear systems. We study stability in the presence of measurement noise and illustrate the tradeoff when selecting the observer gain between state reconstruction speed and robustness to model uncertainty on the one hand versus amplification of noise on the other. Based on this tradeoff we propose a high-gain observer that switches between two gain values. This scheme is able to quickly recover the system states during large estimation error and reduce the effect of measurement noise in a neighborhood of the origin of the estimation error. We argue boundedness and ultimate boundedness of the closed-loop system under switched-gain output feedback.     

The Estimation of the Molecular Weight of Polytetrafluoroethylene Based on the Heat of Crystallisation. A Comment on Suwa's Equation

Summary: The influence of number‐average molecular weight ranging from 1.4 · 10⁴ to 1.2 · 10⁶ g/mol on the thermal behaviour of polytetrafluoroethylene (PTFE) has been studied. Samples have been prepared by radiation‐induced degradation of commercial PTFE. The molecular weight has been calculated using end‐group concentration determined by infrared (IR) spectroscopy and ¹⁹F solid‐state nuclear magnetic resonance (NMR) spectroscopy. Melting and crystallisation heats were investigated by differential scanning calorimetry (DSC). The results have been discussed with respect to quantitative relationships between number‐average molecular weight and heat of crystallisation of PTFE described in the literature. The molecular weight calculated from Suwa's equation, which is often used in the literature, has been found to be too low.

Concentration of end‐groups and the molecular weight of PTFE versus the irradiation dose.     

CuFe2O4 Nanoparticles: A Magnetically Recoverable Catalyst for Selective Deacetylation of Carbohydrate Derivatives

An efficient protocol for the rapid room temperature deacetylation of carbohydrate derivatives using CuFe₂O₄ nanoparticles as an inexpensive and reusable catalyst is presented. After separation of the catalyst with an external magnet, the reaction products are easily obtained in good purity and excellent yields.     

Differences between the isostructural IRMOF-1 and MOCP-L porous adsorbents

Several discrepancies have emerged about the preparation methods of MOF-5 material, one of the adsorbents more studied for hydrogen storage, because different synthesis procedures give rise to apparently isostructural materials but actually having very different textural properties and also showing different hydrogen adsorption capacities. In this work, MOF-5 material has been successfully synthesized according to the two most extended methods, and the respective resulting materials, IRMOF-1 and MOCP-L, have been widely characterized. Powder X-ray diffraction confirmed that MOF-5 is the main crystallized phase obtained in both cases, and that the MOCP-L product contains some ZnO impurities, mainly in the form of segregated crystals that appear preferentially outside the micropores. The presence of ZnO in the MOCP-L material has been also confirmed by other characterization techniques. On the other hand, interpenetrated networks in the MOCP-L material are not significant, appearing in any case at a similar extent than in IRMOF-1; consequently it cannot be considered for explaining the differences observed in the adsorption capacity of both materials. These differences cannot be completely explained simply by the segregated ZnO impurities, so the presence of some ZnO nanocrystals partially blocking the micropores of MOCP-L must be admitted.     

Simultaneous determination of daidzein, equol, genistein and bisphenol A in human urine by a fast and simple method using SPE and GC-MS

Human diet contains weakly estrogenic compounds such as daidzein (DAI) and genistein (GEN), phytoestrogens present in soy and many vegetables as well as bisphenol A (BPA), a contaminant from packing materials and plastic containers for foods and beverages. In light of concerns about hormonally active agents, biomonitoring methods are needed to assess human exposure to such compounds. A method for simultaneous determination of DAI, its metabolite equol (EQ), GEN, and BPA by GC-MS analysis was established, validated and applied to measure concentrations in human urine. Sample preparation involves enzymatic conjugate cleavage, SPE and derivatization by silylation. For GC/MS analysis, deuterated DAI and GEN and( 13)C-BPA are used as internal standards. LOD are 4, 4, 5 and 3 ng/mL urine for DAI, EQ, GEN and BPA, respectively. Interassay variations were 9% for DAI, 15% for EQ, 18% for GEN and 10% for BPA. Simple workup and accuracy of the method are suited for biomonitoring. An analysis of urine samples from 15 adults consuming typical German food revealed dietary exposure to phytoestrogens in all samples: GEN concentrations ranged between 13 and 238 ng/mL, those for DAI ranged from 12 to 356 ng/mL. More than half of the individuals excreted also the more estrogenic metabolite EQ, at levels of 8-128 ng/mL. Higher concentrations (GEN: 820, DAI: 960 and EQ: 1740 ng/mL) were measured in a 24 h urine sample upon ingestion of soy protein (50 g with 12.9 mg DAI and 25.2 mg GEN). Only urine collected after some days on strict phytoestrogen-free diet had undetectable isoflavone levels. BPA was detected in 9 of 15 urine samples ranging from 3 to 11 ng/mL, and at 55 ng/mL in one sample. In conclusion, a reliable method to determine BPA and isoflavones in urine was established and applied in a pilot study: Biomonitoring results show much higher dietary exposure to phytoestrogens than to BPA in German adults.     

Estimation and test for the parameters of the arrhenius model

Estimation and test procedures are given for the parameters of the Arrhenius model. The assumed failure distribution is of exponential or Weibull type with fixed and temperature independent shape parameter. The approach is based on failure terminated experiments on at least two different tempera-ture levels with pre-assigned and equal number of failures for each temperature level. Estimation consists of two steps, modified maximum likelihood estimation followed by ordinary least squares regression. Statistical tests are defined on acceptance regions given in the particular parameter space by circles or ellipses depending only on the experimental design.     

Sidegating in a GaAs MBE-grown HFET structure

The sidegating effect in a MBE-grown HFET GaAs structure is considerable and persists to large distances. On the other hand, the leakage current is very low. A model that takes into account the unusual features of sidegating in this structure and is consistent with other studies of similar structures is proposed. On the basis of the model, a change in the isolation process was implemented. This resulted in a large reduction of the sidegating effect and confirmed the main features of the model