Synthesis,characterisation and properties of α,β-poly(N-2-hydroxyethyl)-dl-aspartamide-graft-maleic anhydride precursors and their stimuli-responsive hydrogels

A family of poly(amino acid)-maleic anhydride hydrogels were designed and synthesized. Water soluble polymeric precursors were prepared by partially substituting the hydroxyl groups of the α,β-poly(N-2-hydroxyethyl)-dl-aspartamide backbone with maleic anhydride, so as to provide double bonds for crosslinking and carboxylic acid groups for pH and electric field responsiveness. Reaction conditions (reactive mixture composition and catalysis) were systematically varied in order to obtain PHEA–MA precursors with different and reliable graft-maleic anhydride levels. PHEA–MA precursors were characterised by titration, Nuclear Magnetic Resonance (¹H NMR), Fourier-Transformed Infrared Spectroscopy (FTIR) and Size Exclusion Chromatography (SEC) for structural and molecular determination. Aqueous solutions of selected PHEA–MA precursors were subjected to gamma-irradiation at different irradiation doses and polymer to water concentrations in order to induce chemical crosslinking without the addition of crosslinking agents. The yield of crosslinking reactions was evaluated by solubility tests as well as the effect of ammonium persulphate, as assistant radical initiator for gamma crosslinking. Selected hydrogels were tested through swelling measurements to prove their pH and electric field responsiveness. Structural features of the different variants produced were related to the swelling behaviour.     

Fast three-dimensional nanoscale metrology in dual-beam FIB–SEM instrumentation

A quantitative surface reconstruction technique has been developed for the geometric characterization of three-dimensional structures by using a combined focused ion beam—scanning electron microscopy (FIB–SEM) instrument. A regular pattern of lines is milled at normal incidence on the sample to be characterized and an image is acquired at a large tilt angle. By analyzing the pattern under the tilted view, a quantitative estimation of surface heights is obtained. The technique has been applied to a test sample and nanoscale resolution has been achieved. The reported results are validated by a comparison with atomic force microscopy measurements.     

Multi-objective optimization by genetic algorithm of structural systems subject to random vibrations

This paper deals with a multi-objective optimization criterion for linear viscous-elastic device utilised for decreasing vibrations induced in mechanical and structural systems by random loads. The proposed criterion for the optimum design is the minimization of a vector objective function. The multi-objective optimization is carried out by means of a stochastic approach. The design variables are the device frequency and the damping ratio. As cases of study, two different problems are analysed: the base isolation of a rigid mass and the tuned mass damper positioned on a multi degree of freedom structural system subject to a base acceleration. The non-dominated sorting genetic algorithm in its second version (NSGA-II) is adopted to obtain the Pareto sets and the corresponding optima for different characterizations of the system and input.     

Minimum reinforcement in concrete structures and material/structural instability

The problem of the assessment of minimum reinforcement in concrete members has been examined both theoretically and experimentally by the bridged crack model. The model has been demonstrated to be an efficient numerical tool for investigating the behavior of structural elements in bending, and allowed to show the minimum reinforcement percentage depends on the structural element size, and decreases with increasing beam depths. In the model, Linear Elastic Fracture Mechanics concepts are used to determine the equilibrium and the compatibility equations of a beam segment subjected to bending in presence of a mode I crack. Recently, the model has been extended to include the presence of closing stresses as a function of the crack opening in addition to steel reinforcement closing traction. This allows to characterize the mechanical behavior of fiber reinforced structural elements. A criterion for accounting for crushing in compression has been introduced as well, to bound from below (minimum reinforcement) and from above (maximum reinforcement) a region of stable and ductile mechanical behavior as a function of the mechanical properties as well as of the size of the structural element. Some experimental results are commented under this light.     

Wireless LANs Systems: An Advanced Resource Assignement Algorithm

The paper describes the design and simulation of a radio modem architecture, which provides wireless access to Internet in a single-hop, ad hoc network.The main emphasis is on the Medium Access Control (MAC) and Dynamic Link Control (DLC) layer’s design, and in particular on the adopted innovative scheduling algorithm, which has been developed to satisfy the system requirements of the WIND-FLEX radio modem. The algorithm is presented and compared to the Earliest Deadline First (EDF) solution.Several software simulation tests have been executed on the network, to test the performance of the system, in order to verify the capability of the scheduler algorithm to satisfy the expected requirements and the efficiency of the implemented solutions. Giuseppe Razzano was born in Roma, Italy, in 1974. He has graduated in Electronic Engineering “summa cum laude” and has received PhD in Communication Systems and Computer Science, in 2004 from University of Rome “La Sapienza”. From 2000 to 2001 he worked as research assistant at VTT electronics, in Finland. From 2004 to 2005 he worked as senior researcher at Forschungzentrum Telekommunikation Wien (Telecommunication Research Centre Vienna) in Austria. Currently, he works as System Engineer for Vitrociset S.p.A., working in a project funded by European Space Agency (ESA), for the development of a new generation space launcher vehicle (VEGA). In the past years, he worked in several projects funded by EC within the IST (Information Societies Technology) program, being also involved in projects in collaboration with Italian companies. His research is focused on resource management algorithms for wireless LANs and cellular networks. He is also interested in object-oriented programming and development methodologies. In these fields, he is author of several papers published in international journals and conferences. Francesco Delli Priscoli was born in Rome in 1962. He graduated in Electronic Engineering “summa cum laude” from the University of Rome “La Sapienza” in 1986. He received the Ph.D. in system engineering from the University of Rome “La Sapienza” in 1991. From 1986 to 1991 he worked in the “Studies and Experimentation” Department of Telespazio (Rome). Since 1991 he is working for the University of Rome “La Sapienza” where, at present, he is “Full Professor” and holds the courses “Automatic Controls”, “System Theory” and “Network Control and Management I and II”. In the framework of his activity, he researches in the nonlinear control theory and in the area of control-based resource management procedures for the third and forth generation of mobile systems. He is the author of about 150 technical papers on the above topics appeared on major international reviews (about 50) and conferences (about 100). In 2000 he has been scientific consultant for the Italian Council of Ministers in the framework of the auction for the assignment of the Italian Universal Mobile Telecommunication System (UMTS) licensees. He is an associate editor of Control Engineering Practice and a member of the IFAC Technical Committee on “Networked Systems”. He is/has been scientific responsible, for the University of Rome “La Sapienza”, of 17 projects financed by the European Union (fourth, fifth and sixth framework programmes) or by the European Space Agency (ESA), dealing with resource management for UMTS and broadband terrestrial and satellite wireless systems. He is also a project evaluator for the European Commission. Roberto Cusani received the “laurea” degree in Electronic Engineering (cum laude) and the Ph.D. in Communication Systems and Computer Science from the University of Rome “La Sapienza”. From 1986 to 1990 he was research engineer at the University of Rome “Tor Vergata”, teaching Digital Signal Processing. In 1991 he joined the University of Rome “La Sapienza” as Associate Professor of Signal Theory. In 2000 he becomes Full Professor and teaches Information Theory and Coding, and Mobile Communications. His former research activities concern transmission and coding of signals and images, with emphasis on random processes, spectral estimation and image coding. Since 1992 he focused his activities in the field of the digital communication systems, with emphasis on channel equalisation and coding for HF and radio-mobile (GSM) links, on the design of CDMA receivers for UMTS and, in general, on the use of digital techniques within telecommunication equipments. More recently his interests also includes the study of MAC (Multiple Access Control) protocols with application to wireless area networks (WLANs), reconfigurable ad-hoc networks and satellite links. He is author of more than 100 publications in international journals and conferences, of the text-book “Teoria dei Segnali” and of five patents regarding telecommunication applications. He was involved in many research programs, both national and international, and in projects with the industrie.     

Mechanical Damping Arising from Dislocation Motion in Sapphire and Ruby Crystals

Internal friction was measured up to the melting point (i.e., T_m≈ 2050°C) in sapphire and two ruby single crystals (chromium content of 0.1 and 0.5 wt%), as a function of strain amplitude, temperature, and composition. The frequency range of the measurements was 6.7–17 Hz. Static (torsional creep) experiments also were performed up to very high temperatures, to provide further phenomenological insight about the mechanism of crystal deformation. Results of dynamic and static experiments were analyzed and discussed in terms of dislocation motion along the basal (0001) plane, perpendicular to which the torsion axes of the crystals were oriented. Particular emphasis was placed on isolating the role of chromium concentration, in regard to impeding dislocation motion and multiplication. An equivalent spring-dashpot model, which attempts to represent the damping results quantitatively, also has been given.     

A simple and rapid assay based on hot water extraction and liquid chromatography–tandem mass spectrometry for monitoring quinolone residues in bovine milk

A rapid, specific and sensitive procedure for determining residues of eight widespread used quinolone antimicrobials in bovine milk is presented. The method is based on the matrix solid-phase dispersion technique with hot water as extractant followed by LC/MS/MS. The entire sample treatment did not take more than 40 min. Hot water appeared to be an efficient extracting medium, since absolute recoveries of the analytes in milk were 77–90%. The method proved to be robust as matrix effects did not affect significantly the accuracy of the method, as evidenced by analyzing six different batches of milk. Using norfloxacin as surrogate analyte, the accuracy of the method at three different spike levels of the analytes in milk was 93–110% with RSDs not larger than 10%. On the basis of a S/N of 10, estimated LOQs of this method range from 0.3 to 1.5 ng/ml, well below the tolerance levels of quinolones in milk set by the European Union.     

Controlling Cellular Uptake by Surface Chemistry,Size, and Surface Topology at the Nanoscale

Cell cytosol and the different subcellular organelles house the most important biochemical processes that control cell functions. Effective delivery of bioactive agents within cells is expected to have an enormous impact on both gene therapy and the future development of new therapeutic and/or diagnostic strategies based on single‐cell–bioactive‐agent interactions. Herein a biomimetic nanovector is reported that is able to enter cells, escape from the complex endocytic pathway, and efficiently deliver actives within clinically relevant cells without perturbing their metabolic activity. This nanovector is based on the pH‐controlled self‐assembly of amphiphilic copolymers into nanometer‐sized vesicles (or polymersomes). The cellular‐uptake kinetics can be regulated by controlling the surface chemistry, the polymersome size, and the polymersome surface topology. The latter is controlled by the extent of polymer–polymer phase separation within the external envelope of the polymersome.