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11.
Critical assessment of extracellular polymeric substances extraction methods from mixed culture biomass 总被引:3,自引:0,他引:3
Extracellular polymeric substances (EPS) have a presumed determinant role in the structure, architecture, strength, filterability, and settling behaviour of microbial solids in biological wastewater treatment processes. Consequently, numerous EPS extraction protocols have recently been published that aim to optimize the trade off between high EPS recovery and low cell lysis. Despite extensive efforts, the obtained results are often contradictory, even when analysing similar biomass samples and using similar experimental conditions, which greatly complicates the selection of an extraction protocol. This study presents a rigorous and critical assessment of existing physical and chemical EPS extraction methods applied to mixed-culture biomass samples (nitrifying, nitritation-anammox, and activated sludge biomass). A novel fluorescence-based method was developed and calibrated to quantify the lysis potential of different EPS extraction protocols. We concluded that commonly used methods to assess cell lysis (DNA concentrations or G6PDH activities in EPS extracts) do not correlate with cell viability. Furthermore, we discovered that the presence of certain chemicals in EPS extracts results in severe underestimation of protein and carbohydrate concentrations by using standard analytical methods. Keeping both maximum EPS extraction yields and minimal biomass lysis as criteria, it was identified a sonication-based extraction method as the best to determine and compare tightly-bound EPS fractions in different biomass samples. Protein was consistently the main EPS component in all analysed samples. However, EPS from nitrifying enrichments was richer in DNA, the activated sludge EPS had a higher content in humic acids and carbohydrates, and the nitritation-anammox EPS, while similar in composition to the nitrifier EPS, had a lower fraction of hydrophobic biopolymers. In general, the easily-extractable EPS fraction was more abundant in carbohydrates and humic substances, while DNA could only be found in tightly bound EPS fractions. In conclusion, the methodology presented herein supports the rational selection of analytical tools and EPS extraction protocols in further EPS characterization studies. 相似文献
12.
Gizem Ozbuyukkaya Robert S. Parker Goetz Veser 《American Institute of Chemical Engineers》2022,68(3):e17538
Accurate chemical kinetics are essential for reactor design and operation. However, despite recent advances in “big data” approaches, availability of kinetic data is often limited in industrial practice. Herein, we present a comparative proof-of-concept study for kinetic parameter estimation from limited data. Cross-validation (CV) is implemented to nonlinear least-squares (LS) fitting and evaluated against Markov chain Monte Carlo (MCMC) and genetic algorithm (GA) routines using synthetic data generated from a simple model reaction. As expected, conventional LS is fastest but least accurate in predicting true kinetics. MCMC and GA are effective for larger data sets but tend to overfit to noise for limited data. LS-CV strongly outperforms these methods at much reduced computational cost, especially for significant noise. Our findings suggest that implementation of CV with conventional regression provides an efficient approach to kinetic parameter estimation with high accuracy, robustness against noise, and only minimal increase in complexity. 相似文献
13.
Focusing on a process where a designer embeds herself in craftspeople’s workshops with the intention of ‘learning from inside’ and ‘making together’; this paper proposes pathways for considering collaboration between designers and craftspeople via digital fabrication and weaving. The tension of forces that make weaving possible, act as an analogy for this collaboration as well as providing the title of the project as ‘One Over, One Under’. The project proposes a mode of working and a spectrum of outcomes where the designer has a first-hand experience of production techniques, engages in a serious and continuous dialogue through making, and develops an intervention that also brings forth her own skill set (introducing digital fabrication and structural variations), thus transforming the conventions and boundaries between established roles and manufacturing techniques. An experiment that resulted in a series of objects considering both the technologies of production and the input of the designer, this process not only increases the potentials that crafts hold for the field of design, but it also offers possibilities of collaboration and a further articulation of the design act. 相似文献
14.
Betul Gizem Acan Omer Said Toker Tugba Aktar Faruk Tamturk Ibrahim Palabiyik Nevzat Konar 《International Journal of Food Science & Technology》2020,55(3):1298-1310
In this study, it is aimed to investigate and evaluate the use of molasses which is a by-product of sugar production as a novel bulking agent in ice cream as a sugar replacer. Sugar beet molasses (75%) and 12 DE maltodextrin (25%) were converted into powder form by spray drying. Spray-dried sugar beet molasses (SDSM) was then used as a substitute of sugar in different ratios (0:100, 25:75, 50:50, 75:25 and 100:0) in the ice cream products. The increased amount of SDSM decreased the overrun, L*, whiteness index (WI) and melting behaviours and increased a*, b*, total phenolic content, consistency and viscosity. Meanwhile, thermal properties have not been affected by the use of SDSM (P < 0.05). The sensory findings have been particularly interesting especially when replacing above 50% sugar with SDSM, aroma, flavour and general acceptability decrease. According to the results of this study, substituting 25% of total sugar with SDSM as a bulking agent can decrease cost of the product and improve total phenolic content and some quality parameters without compromising sensorial properties. 相似文献
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Gizem Korkmaz Chris J. Kuhlman S. S. Ravi Fernando Vega-Redondo 《World Wide Web》2018,21(5):1187-1221
Contagion models have been used to study the spread of social behavior among agents of a networked population. Examples include information diffusion, social influence, and participation in collective action (e.g., protests). Key players, which are typically agents characterized by structural properties of the underlying network (e.g., high degree, high core number or high centrality) are considered important for spreading social contagions. In this paper, we ask whether contagions can propagate through a population that is devoid of key players. We justify the use of Erd?s-Rényi random graphs as a representation of unstructured populations that lack key players, and investigate whether complex contagions—those requiring reinforcement—can spread on them. We demonstrate that two game-theoretic contagion models that utilize common knowledge for collective action can readily spread such contagions, thus differing significantly from classic complex contagion models. We compare contagion dynamics results on unstructured networks to those on more typically-studied, structured social networks to understand the role of network structure. We test the classic complex contagion and the two game-theoretic models with a total of 18 networks that range over five orders of magnitude in size and have different structural properties. The two common knowledge models are also contrasted to understand the effects of different modeling assumptions on dynamics. We show that under a wide range of conditions, these two models produce markedly different results. Finally, we demonstrate that the disparity between classic complex contagion and common knowledge models persists as network size increases. 相似文献
17.
Hadi Nozari Gizem Karaca Onur Tuncer Arif Karabeyoglu 《International Journal of Hydrogen Energy》2017,42(21):14775-14785
Due to its high hydrogen density and extensive experience base, ammonia (NH3) has been gaining special attention as a potential green energy carrier. This study focuses on premixed ammonia–hydrogen–air flames under standard temperature and pressure conditions using an inert silicon-carbide (SiC) porous block as a practical and effective medium for flame stabilization. Combustion experiments conducted using a lab scale burner resulted in stable combustion and high combustion efficiencies at very high ammonia concentration levels over a wide range of equivalence ratios. Noticeable power output densities have also been achieved. Preliminary results of NOx emission measurements indicate NOx concentrations as low as 35 ppm under rich conditions. The remarkable capability of this specific burner to operate efficiently and cleanly at high ammonia concentration levels, which can easily be achieved by partial cracking of NH3, is believed to be a key accomplishment in the development of ammonia fired power generation systems. 相似文献
18.
Gizem Yanalak Abdalaziz Aljabour Emre Aslan Faruk Ozel Imren Hatay Patir 《International Journal of Hydrogen Energy》2018,43(36):17185-17194
The efficiencies of a series of hydrogen evolving catalysts based on metal oxide nanofibers (NiO, Co3O4, Mn3O4) are investigated for the photocatalytic hydrogen evolution from water without using any co-catalyst under the visible light irradiation by using triethanolamine (TEOA) as an electron donor and Eosin-Y (EY) dye as a photosensitizer. It is found that the photocatalytic hydrogen evolution activities follow the order as: Mn3O4<Co3O4<NiO (196 μmolg?1h?1, 5552 μmolg?1h?1, 7757 μmolg?1h?1, respectively). Moreover, the catalytic behavior of these nanofibers on the hydrogen production has been also compared to bulk forms of NiO, Co3O4 and Mn3O4 by producing hydrogen 937 μmolg?1h?1, 901 μmolg?1h?1 and 135 μmolg?1h?1, respectively. The nanofiber structures demonstrated much higher photocatalytic activity than bulk forms due to the effect of the increased surface to volume ratio deduced from the fibrous character. The photocatalytic plausible pathway for the hydrogen production is also discussed. 相似文献
19.
Therapeutic Potential of Myrtus communis Subsp.communis Extract Against Acetic ACID‐Induced Colonic Inflammation in Rats
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Yüzüak Gizem Durak Yüzüak Ercüment Teichert Niclas Hütten Andreas Elerman Yalçın 《Metallurgical and Materials Transactions A》2017,48(5):2654-2659
Metallurgical and Materials Transactions A - Zr-Co is one of the essential magnetic materials due to its interesting magnetic and structural properties. In this work, we studied the magnetic and... 相似文献