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51.
Corals, like many other coelenterates, contain fluorescent pigments that show considerable homology with the well known green fluorescent protein of the jellyfish Aequoria. In corals, unlike jellyfish, multiple proteins are present and the range of excitations and emissions suggest the possibility of energy transfer. The occurrence of F?rster resonant energy transfer (FRET) between fluorescent proteins in corals has already been reported and time-resolved spectra have shown the effect on fluorescent lifetime, but without any spatial resolution. Lifetime confocal microscopy offers lower time resolution but excellent spatial resolution. Lifetimes of the isolated A. millepora pigments amilFP490, amilFP504, and amilFP593 (names indicate emission peaks) were 2.8, 2.9, and 2.9 ns, respectively. In the coral sample, imaging the entire emission spectrum from 420 nm, the mean lifetime was reduced to 1.5 ns, implying that FRET was occurring. Looking just at the fluorescence from FRET donors the lifetime was even shorter, at 1.3 ns, supporting this interpretation. In contrast, no reduction in lifetime is seen in the coral Euphyllia ancora, where the pigment distribution also suggests that the pigments are unlikely to be involved in photoprotection. This study set out to determine the extent of FRET between pigments in two corals, Acropora millepora and Euphyllia, ancora which differ in the arrangement of their pigments and hence possibly in pigment function. 相似文献
52.
The character of transient electric fields induced in spherical homogeneous tissue models of man and animals by incident electromagnetic fields similar to that originating from lightning and EMP generators has been theoretically determined. The magnitude of the induced fields is found to be approximately proportional to the time rate of change of the incident field and independent of the pulsewidth. It was also found that maximum EMP induced field in spherical shaped bodies is nearly proportional to the radius of the body. Therefore, for EMP safety standards, more consideration should be given to the time rate of change of the incident field rather than to the pulsewidth, and when laboratory animal data are used as a guide they should be extrapolated to account for body size. 相似文献
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Multicast multi-layered communications must implement efficient control algorithms to address undesirable network behaviors. This paper proposes two multi-metric algorithms for computing the rates of the video layers and improve the global video quality of a multicast session. In fact, we show that a single-metric approach may degrade some network parameters without obtaining any substantial improvements. Our first algorithm combines three metrics and a set of weights in such a way that one metric can be prioritized. This leads to an improved quality of multicast sessions, as we show through a number of experiments. In networks where the available resources are highly variable, however, the stability of the video quality is compromised if absolute values of the metrics are adopted. We then propose a second algorithm that uses the relative values of the metrics on a per-entry basis. Computation of the global quality of the multicast session is based on a differential matrix that stores the metrics for each receiver. This scheme takes into account the dynamics of the available resources and the heterogeneity of receivers. The great benefit of this approach is that the global video quality is always improved for every loop of the algorithm. 相似文献
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Higher-Order and Symbolic Computation - 相似文献
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Grimandi G Soueidan A Anjrini AA Badran Z Pilet P Daculsi G Faucheux C Bouler JM Guicheux J 《Microscopy research and technique》2006,69(8):606-612
The increased generation and up-regulated activity of bone resorbing cells (osteoclasts) play a part in the impairment of bone remodeling in many bone diseases. Numerous drugs (bisphosphonates, calcitonin, selective estrogen receptor modulators) have been proposed to inhibit this increased osteoclastic activity. In this report, we describe a pit resorption assay quantified by scanning electron microscopy coupled with image analysis. Total rabbit bone cells with large numbers of osteoclasts were cultured on dentin slices. The whole surface of the dentin slice was scanned and both the number of resorption pits and the total resorbed surface area were measured. Resorption pits appeared at 48 h and increased gradually up to 96 h. Despite the observation of a strong correlation between the total resorption area and the number of pits, we suggest that area measurement is the most relevant marker for osteoclastic activity. Osteotropic factors stimulating or inhibiting osteoclastic activity were used to test the variations in resorption activity as measured with our method. This reproducible and sensitive quantitative method is a valuable tool for screening for osteoclastic inhibitors and, more generally, for investigating bone modulators. 相似文献
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Bernard Charra Guillaume Jean Charles Chazot Thierry Vanel Jean-Claude Terrat Guy Laurent 《Hemodialysis international. International Symposium on Home Hemodialysis》1999,3(1):16-22
Long, slow hemodialysis (3 × 8 hours/week) has been used without significant modification in Tassin, France, for 30 years with excellent morbidity and mortality rates. A long dialysis session easily provides high Kt/Vurea and allows for good control of nutrition and correction of anemia with a limited need for erythropoietin (EPO). Control of serum phosphate and potassium is usually achieved with low-dose medication. The good survival achieved by long hemodialysis sessions is essentially due to lower cardiovascular morbidity and mortality than in short dialysis sessions. This, in turn, is mainly explained by good blood pressure (BP) control without the need for antihypertensive medication. Normotension in this setting is due to the gentle but powerful ultrafiltration provided by the long sessions, associated with a low salt diet and moderate interdialytic weight gains. These allow for adequate control of extracellular volume (dry weight) in most patients without important intradialytic morbidity. Therefore, increasing the length of the dialysis session seems to be the best way of achieving satisfactory long-term clinical results. 相似文献
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