Upregulation of TLRs and IL-6 as a Marker in Human Colorectal Cancer

Toll-like receptors (TLRs) not only form an important part of the innate immune system but also serve to activate the adaptive immune system in response to cancer. Real-time PCR; immunohistochemical stain and Western blotting analyses were performed to clarify molecular alterations in colorectal cancer (CRC) patients. We identified Toll-like receptor 1 (TLR1), TLR2, TLR4 and TLR8 gene expression levels and downstream gene, i.e., interleukin-6 (IL-6), IL-8, interferon-α (IFN-α) and myeloid differentiation primary-response protein-88 (MyD88), expression levels in CRC patients and in cancer cell lines. CRC tissues have higher TLR1, TLR2, TLR4, TLR8, IL-6 and IL-8 gene expression levels than do the normal colon mucosa (p < 0.05). TLR2 expression varied in different cell types (mucosa and lymphocytes). There was no difference in the MyD88 and IFN-α gene expression levels between cancerous and normal colon mucosa. CRC patients had higher levels of IL-6 (p = 0.002) and IL-8 (p = 0.038) expression than healthy volunteers did; and higher IL-6 and IL-8 expression was also found to signify a higher risk of recurrence. CL075 (3M002) treatments can reduce the production of IL-8 in different cancer cell lines. The signaling pathway of TLRs in cancer tissue is different from that in normal cells; and is MyD88-independent. Higher expression levels of TLR1, TLR2, TLR 4 and TLR 8 mRNA were related to upregulation inflammatory cytokines IL-6 and IL-8 gene expression in tissue and to the upregulation of IL-6 in blood. The concentration of IL-6 in serum can be used as an indicator of the possibility of CRC recurrence. Treatment with 3M002 can reduce IL-6 production in vitro and may prevent CRC recurrence. Our findings provide evidence that TLR1, TLR2, TLR4 and TLR8 gene expression induce downstream IL-6 and IL-8 gene expression; detection of these expression levels can serve as a CRC marker.     

Heat and Mass Transfer Measurements for Tray-Fermented Fungal Products

In this study, heat and mass transfer in static tray fermentation, which is widely used in solid-state fermentation (SSF) to produce fungal products, such as enzymes or koji, is investigated. Specifically, kinetic models of transport phenomena in the whole-tray chamber are emphasized. The effects of temperature, moisture, and humidity on microbial growth in large-scale static tray fermentation are essential to scale-up SSF and achieve uniform fermentation. In addition, heat and mass transfer of static tray fermentation of Trichoderma fungi with two tray setups??traditional linen coverings and stacks in a temperature?Chumidity chamber is examined. In both these setups, the following factors of fermentation were measured: air velocity, air temperature, illumination, pH, carbon dioxide (CO₂) concentration, and substrate temperature, and the effects of bed height, moisture of substrate, and relative humidity of air are studied. A thin (1 cm) bed at 28 °C and 95 % relative humidity is found to be optimum. Furthermore, mixing was essential for achieving uniform fermentation of Trichoderma fungi. This study has important applications in large-scale static tray fermentation of fungi.     

Polymer electrolyte membranes composed of an electrospun poly(vinylidene fluoride) fibrous mat in a poly(4‐vinylpyridine) matrix

Newly proposed polymer electrolyte membranes (PEMs) composed of an electrospun poly(vinylidene fluoride) (PVDF) fibrous mat embedded in a poly(4‐vinylpyridine) (P4VP) matrix were successfully fabricated in order to improve the mechanical and dimensional stabilities and ionic conductivity of membranes in lithium rechargeable batteries. Fourier transform infrared spectroscopic analysis showed that as a result of the use of a high voltage during electrospinning the crystalline structure of PVDF changed partially from α‐phase to β‐phase. Energy‐dispersive X‐ray spectroscopy confirmed the existence of crosslinked P4VP in the PVDF fibrous mat. The electrolyte uptakes of PVDF and PVDF/P4VP composite mats were higher than that of PVDF cast film. The tensile properties of PVDF/P4VP composite mat were considerably improved compared to those of the pristine PVDF fibrous mat under both dry and wet (soaked with electrolyte) conditions. In addition, the mechanical and dimensional stabilities of the PVDF/P4VP composite PEM were further enhanced due to crosslinking between the P4VP chains. Furthermore, the PVDF/P4VP composite PEM exhibited an ionic conductivity that was an order of magnitude higher than that of traditional PVDF film. © 2012 Society of Chemical Industry     

A MODIFIED FINITE-ELEMENT SMEARING TECHNIQUE FOR FREE-EDGE STRESS ANALYSIS

A new finite-element smearing scheme is presented in the present study to provide a practical yet accurate free-edge stress analysis. It is proposed that, in the area away from the free edge of the laminate, all layers are to be smeared into one layers of finite elements, while near the free edge each layer is to be modeled as one layer of finite elements. Compatibility between the smeared elements andthe regular elements is enforcedby the penalty method. The present technique can be applied to general laminates with various geometries, loadings, etc. The results of two example problems can show that the present approach is simple, accurate, and economical in computing free edge stresses of laminates.     

Analyses of distortions and cross modulations in erbium-doped fiberamplifiers

The closed-form expressions of nonlinear Fourier transfer functions are presented for the analyzes of distortions and cross modulations in erbium-doped fiber amplifier (EDFA) systems. Based upon Volterra series and the time-dependent perturbation theory, the transfer functions are obtained after taking account of both the EDFA nonlinearity and chirping effect. Their applications to an AM-SCM and an eight-channel wavelength-division-multiplexed systems are demonstrated     

Effect of XeF laser treatment on structure of nanocrystalline diamond films

Nanocrystalline diamond (NCD) films were deposited on Si substrates by microwave plasma-enhanced chemical vapor deposition (MPECVD) using methane/hydrogen/oxygen (30/169/0.2 sccm) as process gases. Subsequently a thin (0.33 μm) and a thick (1.01 μm) NCD films were irradiated with XeF excimer laser (λ = 351 nm) with 300 and 600 mJ cm^? 2 of energy densities in air. The NCD films became rougher after laser irradiations. Fraction of graphitic clusters decreased but oxygen content increased in the thin NCD film after laser irradiation. Opposite phenomena were observed for the thick NCD films. Effect of laser irradiation to oxygenation and graphitization of NCD films was correlated with structural properties of free surface and grain boundaries of the thin and thick NCD films.     

Programmable splice-free quasi-monolithic 2 Gbit/s optical fibre pulse code generator

A mechanically programmable optical fibre lattice pulse code generator, implemented in a quasi-monolithic, splice-free polished coupler technology, has been designed, constructed and characterised. The prototype system permits the generation of reconfigurable four bit pulse sequences with a pulse repetition rate of 2 GHz, i.e. 500 ps interval within the pulses in the output train.<>     

An efficient VLSI switch-box router

A tool for switch-box routing that can route regions with cyclic constraints and with terminals on three or four sides is presented. A divide-and-conquer algorithm is used to explore the greedy channel routing idea, using techniques such as routing area partitioning, dynamic routing strategies, and sweeping concurrent bidirectional columns. The routing area is decomposed into three parts by two special lines cut parallel, making routing easier. The algorithm completely routes Burstein's switch-box problem and with an extension also routes the Deutsch channel example in 19 tracks     

Studies of suppression of the reflected wave and beam-scanning features of the antenna arrays

This paper describes a two-directional linear scanned design by integrating a short leaky-wave antenna (LWA) with aperture-coupled patch antenna arrays. This architecture proposes a technique not only having the advantage of suppressing the back-lobe due to the reflected wave in the short LWA but also producing two separate linearly scanned beams, each of them radiating in a different region of space (in both the front side and backside of the LWA). In this design, most of the reflected wave of the short LWA is coupled to the patch antenna arrays on the backside of the substrate. The phase of this coupled signal to each antenna element is adjusted by tuning the individual phase shifter in order to control electronically the patch antenna main beam in the cross plane (x<0). Meanwhile, on the front side, the main beam of the short LWA can be simultaneously scanned in the elevation plane (x>0) by changing the operating frequency. Hence, the two linear beam-scanning radiation patterns of individual direction can be created independently, including a narrow beam in the elevation plane (xy plane at x>0) at the front side and a broadside beam in the cross plane (xz plane at x<0) on the backside. The measured results show that the reflected wave of the short LWA in the proposed design is suppressed 8 dB as compared with a traditional short LWA without the aperture-coupled antenna arrays at 10.5 GHz. As a result, this novel architecture provides more flexibility both in the upward elevation plane (H plane) and the downward cross plane (backside-E plane) for possible beam-scanning applications in microwave communications and remote identification.     

Efficient weighted least-squares algorithm for the design of FIRfilters

The weighted least-squares (WLS) technique has been widely used for the design of digital FIR filters. In the conventional WLS, the filter coefficients are obtained by performing a matrix inverse operation, which needs computation of O(N³). The authors present a new WLS algorithm that introduces an extra frequency response including implicitly the weight function. In the new algorithm, the filter coefficients can be solved just by a matrix vector multiplication. It reduces the computational complexity from O(N³ ) to O(N²)