Discovery of a Short‐Chain Dehydrogenase from Catharanthus roseus that Produces a New Monoterpene Indole Alkaloid

Plant monoterpene indole alkaloids, a large class of natural products, derive from the biosynthetic intermediate strictosidine aglycone. Strictosidine aglycone, which can exist as a variety of isomers, can be reduced to form numerous different structures. We have discovered a short‐chain alcohol dehydrogenase (SDR) from plant producers of monoterpene indole alkaloids (Catharanthus roseus and Rauvolfia serpentina) that reduce strictosidine aglycone and produce an alkaloid that does not correspond to any previously reported compound. Here we report the structural characterization of this product, which we have named vitrosamine, as well as the crystal structure of the SDR. This discovery highlights the structural versatility of the strictosidine aglycone biosynthetic intermediate and expands the range of enzymatic reactions that SDRs can catalyse. This discovery further highlights how a sequence‐based gene mining discovery approach in plants can reveal cryptic chemistry that would not be uncovered by classical natural product chemistry approaches.     

Effects of chloride and sulfate on the rate of oxidation of ferrous ion by H2O2

The rates of oxidation of Fe(II) by H(2)O(2) in the presence of sodium perchlorate, sodium nitrate, sodium chloride and sodium sulfate salts (0-1M) have been compared in the study. Experiments were carried out in a batch reactor, in the dark, at pH <3, 25+/-0.5 degrees C and at controlled ionic strength (< or =1M). The experimental results showed that the rates of oxidation of Fe(II) in the presence of chloride, nitrate and perchlorate were identical. In the presence of sulfate, the rate of oxidation of Fe(II) was faster and depended on the pH and the concentration of sulfate. The pseudo second-order rate constants for the reaction of H(2)O(2) with Fe(2+), FeCl(+) and FeSO(4) were determined as 55+/-1, 55+/-1 and 78+/-3 M(-1) s(-1), respectively.     

A Review on the Separation of Niobium and Tantalum by Solvent Extraction

Niobium and tantalum are found together in natural minerals. Leaching with concentrated acid solution or alkaline roasting followed by water/dilute acid leaching results in a solution containing these two metals. In this work, we reviewed the extraction and separation of Nb(V) and Ta(V) from leach liquors in the absence and presence of hydrofluoric acid by acidic, neutral extractants and amines. Several solvent extraction systems were compared in the light of the requirement of hazardous chemicals, extraction/separation efficiency, and stability/solubility of extractants. Since the cationic species of Nb(V) and Ta(V) are unstable in the aqueous solution, the application of acidic extractants for extraction and separation of these metals is limited. Amines can extract Nb(V) as well as Ta(V) irrespective of the presence of fluoride ion but the separation of these metals from the loaded amines should be carried out in stripping process, resulting in process complexity. Another disadvantage encountered for amine is related to high viscosity in extraction process. Neutral extractants are considered as promising extractants which are effective to separate Ta(V) and Nb(V) from either non-fluoride or to moderate fluoride ion concentration in terms of extraction, separation and stripping efficiency. Compared to the traditional methyl iso-butyl ketone (MIBK) based system, methyl isoamyl ketone (MIAK) is found to be a promising extractant to separate Nb(V) and Ta(V).     

Biochemical characterisation of a glycogen branching enzyme from Streptococcus mutans: Enzymatic modification of starch

A gene encoding a putative glycogen branching enzyme (SmGBE) in Streptococcus mutans was expressed in Escherichia coli and purified. The biochemical properties of the purified enzyme were examined relative to its branching specificity for amylose and starch. The activity of the approximately 75 kDa enzyme was optimal at pH 5.0, and stable up to 40 °C. The enzyme predominantly transferred short maltooligosyl chains with a degree of polymerization (dp) of 6 and 7 throughout the branching process for amylose. When incubated with rice starch, the enzyme modified its optimal branch chain-length from dp 12 to 6 with large reductions in the longer chains, and simultaneously increased its branching points. The results indicate that SmGBE can make a modified starch with much shorter branches and a more branched structure than to native starch. In addition, starch retrogradation due to low temperature storage was significantly retarded along with the enzyme reaction.     

Effect of Storage Conditions on 2–Acetyl-1–pyrroline Content in Aromatic Rice Variety, Khao Dawk Mali 105

ABSTRACT: The effect of package and temperature on 2–acetyl-1–pyrroline content in milled aromatic rice during storage was investigated. 2 -Acetyl-1 -pyrroline content was decreased faster at higher storage temperature. However, fat acidity of rice was increased during storage and inversely correlated with 2–acetyl-1–pyrroline content at an early stage of storage. The difference in 2–acetyl-1 -pyrroline recovery from the samples, which were extracted with ethanol at 40 °C and 75 °C, revealed that the starch bound and free forms of 2–acetyl-1–pyrroline may occur in aromatic rice. These results suggested that the biosynthesis of 2–acetyl-1 -pyrroline before starch structure formation in rice kernel could play a key role in the aroma quality of aromatic rice.     

D0C: A code to calculate scalar one-loop four-point integrals with complex masses

We present a new Fortran code to calculate the scalar one-loop four-point integral with complex internal masses, based on the method of 't Hooft and Veltman. The code is applicable when the external momenta fulfill a certain physical condition. In particular it holds if one of the external momenta or a sum of them is timelike or lightlike and therefore covers all physical processes at colliders. All the special cases related to massless external particles are treated separately. Some technical issues related to numerical evaluation and Landau singularities are discussed.     

Two channel digital watermarking for music based on exponential time-spread echo kernel

To robustly and imperceptibly embed copyright information as a watermark into music clips, a two channel digital watermarking system based on the least significant bit (LSB) method and echo hiding (EH) with a new exponential time-spread echo kernel (ETEK) is proposed. By spreading the echo so that it seems like natural room reverberation, as well as the use of two channels to separately watermark by the LSB method and the EH method which have complementary characteristics, the system provides satisfactory sound quality and exhibits both efficient retrieval capacity and robustness against attacks. Experiments show that the proposed system retrieves watermarks with 100% accuracy for all clips and is almost imperceptible. With a set of 42 attacks supplied by the StirMark Benchmark for Audio (SMBA), the proposed system has an average robustness of 0.98 where the ideal value is 1.00, and higher than any conventional methods. With these results, the proposed system is shown to be possible for the application to music distribution over the Internet.     

Magnetoelectric sensor for microtesla magnetic-fields based on (Fe80Co20)78Si12B10/PZT laminates

The magnetoelectric sensor based on (Fe₈₀Co₂₀)₇₈Si₁₂B₁₀/PZT laminates is designed, fabricated and characterized for determining dc and ac magnetic-field strengths as well as field orientations. At low dc magnetic-fields, a ME-voltage response (dV_ME/dH) as high as 2 mV/Oe is achieved. The linear relation V_ME(h_ac) with a slope of dV_ME/dh_ac of 17 mV/Oe shows a great ability to self-powered detecting low ac magnetic-fields. The field orientation can be detected by using the sinusoidal dependence of the magnetoelectric voltage. The sensor is promising not only for microtesla magnetic-field sensing but also for magnetic biosensor applications.     

A comparison of historical land-use change patterns and recommendations for flood plain developments in three delta regions in Southeast Asia

Historical land-cover changes in three delta regions in Southeast Asia are assessed by using selected Landsat images from 1990 to 2005. Potential inundation areas of the delta regions for different return periods are estimated by using digital elevation model data and extreme-value distribution of annual maximum river discharges. The results indicate significant changes of natural land cover within the potential inundation areas due to agricultural exploitation and industrialization. Implications for flood-mitigation policies are discussed. There is a clear need for sustainable land-use management strategies in all three areas.