N ‐acetylcysteine–PLGA nano‐conjugate: effects on cellular toxicity and uptake of gadopentate dimeglumine

Gadolinium as a contrast agent in MRI technique combined with DTPA causes contrast induced nephropathy (CIN) and nephrogenic systemic fibrosis (NSF) which can reduce by usage of antioxidants such as N‐acetyl cysteine by increasing the membrane''s permeability leads to lower cytotoxicity. In this study, N ‐acetyl cysteine‐PLGA Nano‐conjugate was synthesized according to stoichiometric rules of molar ratios andafter assessment by FTIR, NMR spectroscopy and Atomic Force Microscopy (AFM) imaging was combined with Magnevist® (gadopentetate dimeglumine) and its effects on the renal cells were evaluated. MTT [3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide] and cellular uptake assays have indicated relatively significant toxicity of magnevist (P  < 0.05) on three cell lines including HEK293, MCF7 and L929 compared to other synthesized ligands that shown no toxicity. Moreover, systemic evaluation has shown no notable changes of blood urea nitrogen (BUN) and creatinine in kidney of mice. In consequence, antioxidant effect was increased as well as the renal toxicity of the contrast agent reduced at the cell level. As a result, PLGA‐NAC nano‐conjugate can be a promising choice for decreasing the magnevist toxicity for treatment and prevention of CIN and will be able to open a new horizon to research on reduction of toxicity of contrast agents by using nanoparticles.Inspec keywords: blood, toxicology, nanofabrication, cellular biophysics, biomedical materials, nanoparticles, chromatography, cancer, biodegradable materials, biomedical MRI, kidney, pH, nanomedicine, patient treatment, diseases, atomic force microscopy, Fourier transform infrared spectraOther keywords: cellular toxicity, gadopentate dimeglumine, contrast agent, magnetic resonance imaging technique, diethylenetriamine pentaacetate, contrast‐induced nephropathy, nephrogenic systemic fibrosis, stoichiometric rules, molar ratios, dimethyl sulphoxide solution, chromatography techniques, nuclear magnetic resonance spectroscopy, atomic force microscopy imaging, Magnevist®, gadopentetate dimeglumine, renal cells, MTT cytotoxicity, human embryonic kidney‐293, L929 cell lines, in vitro conditions, cellular uptake assays, Magnevist uptake, antioxidant effect, renal toxicity, cell level, PLGA nanocarrier, acetylcysteine nanoconjugate, Magnevist toxicity, N‐acetylcysteine–PLGA nano‐conjugate, N‐acetyl cysteine‐poly‐lactic‐co‐glycolic acid nanoconjugate     

Study of oil uptake and some quality attributes of potato chips affected by hydrocolloids

The use of coating agents is one effective way to reduce oil absorption in fried products. Reducing the fat content of fried foods by application of coatings is an alternative solution to comply with both health concerns and consumer preferences. The aim of this study was to analyze the effect of hydrocolloids as coating agent on the quantity of oil uptake and on sensory attributes of potato chips. The effect of the coating composition showed that the minimum fat content was related to 1% carboxymethyl cellulose (CMC), 0.5% xanthan, 0.3% guar and 1% xanthan with 21.2, 21.7, 22.4 and 24.8%, respectively, and the highest of fat content was related to blank sample (non-coated), 2% tragacanth, 0.5% guar gum with 49.4, 41.7 and 33.2% of oil content, respectively (p <0.05). The most effective coating agent reduced the oil uptake by 57.03, 55.94, 54.67 and 49.71%, respectively (p <0.05). Sensory evaluation showed that the best color was related to 1% CMC, 0.3% guar and 2% tragacanth, and with respect to flavor evaluation the best flavor was observed in tragacanth 2%, CMC 0.5% and CMC 0.1%, and the best texture referred to tragacanth 2%, CMC 0.5% and CMC 1%. In sensory evaluation, all coated chips got high scores compared with blank (non-coated chips) samples (p <0.05).     

A comparative study between gravel and rubber drainage columns for mitigation of liquefaction hazards

Liquefaction is one of the most destructive natural hazards that cause damage to engineering structures during an earthquake. This study aims to examine the effect of rubber and gravel drainage columns on the reduction of liquefaction potential of saturated sandy soils using a shaking table. Experiments were carried out in various conditions such as construction materials, different arrangements and diameters of drainage columns. Effects of the relative density and the input motion on the base test were investigated as well. The results demonstrate that rubber drainage columns have slightly better performance compared to gravel drainage columns at high relative density and high input acceleration. Soil improvement using gravel drainage columns, which leads to reduction in liquefaction effects at moderate input acceleration and low relative density, is a more effective method than that using rubber drainage columns. By increasing the number and diameter of gravel and rubber drainage columns, deformations due to liquefaction are reduced. The drainage rate of gravel drains is higher than that of rubber drains after shaking. Totally, the outcomes indicate that densification is the most important factor controlling liquefaction.     

钢筋与钢纤维混凝土的黏结滑移性能及其关系模型

基于内贴应变片的钢筋与钢纤维混凝土局部黏结试件的拉拔试验,研究钢筋与不同强度钢纤维混凝土的黏结性能。通过对实测钢筋应变的分析,建立了以三次多项式表达的黏结应力分布函数,得到了各级荷载作用下钢筋与钢纤维混凝土黏结应力和相对滑移沿黏结区段的分布,进一步分析了钢纤维和混凝土强度对黏结性能的影响。结果表明,随钢纤维的体积率和混凝土强度的增大,黏结试件加载端附近的黏结应力提高,黏结应力极值总体向加载端靠拢;同时,加载端与自由端的滑移减小。最后,提出了能够较好反映钢筋与钢纤维混凝土受力过程的黏结-滑移关系模型。     

Acoustic emission characterization of damage in short hemp‐fiber‐reinforced polypropylene composites

This article aims at investigating the effects of hygrothermal aging on the damage mechanisms of short white Hemp Fiber Reinforced Polypropylene (HFRP) composites with various fiber contents (10, 20, 30, and 40 wt%). Injected molded specimens were subjected to hygrothermal aging with a relative humidity of 80% and two temperatures, 25 and 50°C. The water absorption and its effect on tensile properties of HFRP composites were investigated. The Acoustic Emission (AE) technique combined with scanning electron microscopy observations was used to identify microstructural damage events leading to overall failure of the HFRP composites. This identification according to hemp‐fiber content and hygrothermal aging was made with an unsupervised method based on a statistical multi‐variable analysis (k‐means algorithm). The AE results indicate that the quality of fiber‐matrix interface plays a major role in the damage process of HFRP composites, shown by the number of AE signals induced by the interface failure and their amplitude ranges. POLYM. COMPOS. 37:1101–1112, 2016. © 2014 Society of Plastics Engineers     

Investigation of Crosstalk and Coupling Effects of Incident Plane Waves in Orthogonal Microstrips in High-Speed Integrated Circuits Using Full-Wave and Quasi-Static Methods

The crosstalk and coupling of the external fields on orthogonal microstrip transmission lines in different layers have significant effects on signal quality in MMIC and PCBs. In this paper the crosstalk is analyzed in detail using both full-wave and quasi-static methods. The used full wave method is mixed potential integral equation method of moment (MPIEMoM). Because of the weak coupling between lines, the effect of the incident plane-wave is studied by applying transmission line theory in a scattered voltage formulation uses quasi-TEM propagation model for each interconnection and the exact distribution of the incident electric field within the layers. Afterward, by using the predetermined lumped circuit model of the cross-region, the effect of coupling between two lines is calculated and then applied to terminal voltages in 1–20 GHz frequency range which results in the final terminal voltages.     

Simultaneous Voltammetric Determination of Synthetic Colorants in Foods Using a Magnetic Core–Shell Fe<Subscript>3</Subscript>O<Subscript>4</Subscript>@SiO<Subscript>2</Subscript>/MWCNTs Nanocomposite Modified Carbon Paste Electrode

This paper reports the production of a novel magnetic nanocomposite based on multi-walled carbon nanotubes (MWCNTs) decorated with magnetic core–shell Fe₃O₄@SiO₂ nanoparticles which were used to fabricate a modified carbon paste electrode (Fe₃O₄@SiO₂/MWCNT-CPE). The Fe₃O₄@SiO₂/MWCNT-CPE was investigated for the simultaneous determination of sunset yellow (SY) and tartrazine (TT) in 0.1 mol/L phosphate buffer solution (pH 6.0) using square wave voltammetry (SWV). The synthesized nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, energy dispersive spectroscopy and Fourier transform infrared spectroscopy. The proposed electrode exhibits linear ranges of 0.5–100 μmol/L SY and TT with detection limits of 0.05 and 0.04 μmol/L for SY and TT, respectively. The novel proposed voltammetric method was successfully applied in the simultaneous determination of SY and TT in food products, with results similar to those obtained using a HPLC method at 95 % confidence level.

Open image in new window

Graphical Abstract A magnetic nanocomposite based on MWCNTs decorated with core–shell Fe₃O₄@SiO₂ was prepared and showed good ability to distinguish the response of SY and TT

     

Constrained grinding optimization for time,cost, and surface roughness using NSGA-II

Selection of parameters in machining process significantly affects quality, productivity, and cost of a component. This paper presents an optimization procedure to determine the optimal values of wheel speed, workpiece speed, and depth of cut in a grinding process considering certain grinding conditions. Experimental studies have been carried out to obtain optimum conditions. Mathematical models have also been developed for estimating the surface roughness based on experimental investigations. A non-dominated sorting genetic algorithm (NSGA II) is then used to solve this multi-objective optimization problem. The objectives under investigation in this study are surface finish, total grinding time, and production cost subjected to the constraints of production rate and wheel wear parameters. The Pareto-optimal fronts provide a wide range of trade-off operating conditions which an appropriate operating point can be selected by a decision maker. The results show the proposed algorithm demonstrates applicability of machining optimization considering conflicting objectives.     

Power Sharing Improvement in Standalone Microgrids with Decentralized Control Strategy

Abstract—This article proposes a method that enables ideal sharing of reactive power among converter-based micro-sources in a microgrid with a decentralized control strategy. Power sharing must be properly performed among micro-sources to avoid circulating currents or overloading. Using conventional droop characteristics to achieve power sharing is not satisfactory, as system asymmetry will greatly impact the quality of sharing. In the proposed method, droop characteristic parameters are modified upon a change in system operating point such that ideal sharing takes place after the modification process. Simulation results are presented to show the validity of analysis.     

Control Optimization of a Slotted Switched Reluctance Generator for High-torque Applications

This article describes the control optimization of a slotted switched reluctance generator for low-speed applications. The machine is simulated with the finite-element method. The turn-off and turn-on angles are optimized by means of a genetic algorithm to maximize the output power and minimize the torque ripple. First, these two criteria are optimized separately with a mono-objective genetic algorithm, and then a multi-objective Pareto genetic algorithm is used. An analysis of optimized parameters and resulting current waveforms is also performed. Experimental results based on a 6/4 switched reluctance generator are also presented.