Preparation of Lung-Targeting,Emodin-Loaded Polylactic Acid Microspheres and Their Properties

Emodin (1,3,8-trihydroxy-6-methylanthraquinone) has been identified to have the potential to improve lung fibrosis and lung cancer. To avoid the liver and kidney toxicities and the fast metabolism of emodin, emodin-loaded polylactic acid microspheres (ED-PLA-MS) were prepared and their characteristics were studied. ED-PLA-MS were prepared by the organic phase dispersion-solvent diffusion method. By applying an orthogonal design, our results indicated that the optimal formulation was 12 mg/mL PLA, 0.5% gelatin, and an organic phase:glycerol ratio of 1:20. Using the optimal experimental conditions, the drug loading and encapsulation efficiencies were (19.0 ± 1.8)% and (62.2 ± 2.6)%, respectively. The average particle size was 9.7 ± 0.7 μm. In vitro studies indicated that the ED-PLA-MS demonstrated a well-sustained release efficacy. The microspheres delivered emodin, primarily to the lungs of mice, upon intravenous injection. It was also detected by microscopy that partial lung inflammation was observed in lung tissues and no pathological changes were found in other tissues of the ED-PLA-MS-treated animals. These results suggested that ED-PLA-MS are of potential value in treating lung diseases in animals.     

解析防排烟设计技术在高层建筑中的应用

随着经济社会的发展,高层建筑日益增多,高层建筑防排烟设计问题逐渐引起人们的关注。文章通过分析高层防排烟设计中存在的问题,对高层建筑防排烟系统的设计方法进行了深入地探讨。     

Functional Characterization of Ubiquitination Genes in the Interaction of Soybean—Heterodera glycines

Ubiquitination is a kind of post-translational modification of proteins that plays an important role in plant response to biotic and abiotic stress. The response of soybean GmPUB genes to soybean cyst nematode (SCN, Heterodera glycines) infection is largely unknown. In this study, quantitative real-time PCR (qRT-PCR) was performed to detect the relative expression of 49 GmPUB genes in susceptible cultivar William 82 and resistant cultivar Huipizhi after SCN inoculation. The results show that GmPUB genes responded to cyst nematode infection at 1 day post-inoculation (dpi), 5 dpi, 10 dpi and 15 dpi. The expression levels of GmPUB16A, GmPUB20A, GmCHIPA, GmPUB33A, GmPUB23A and GmPUB24A were dramatically changed during SCN infection. Furthermore, functional analysis of these GmPUB genes by overexpression and RNAi showed that GmPUB20A, GmPUB33A and GmPUB24A negatively regulated soybean resistance under SCN stress. The results from our present study provide insights into the complicated molecular mechanism of the interaction between soybean and SCN.     

Mitochondrial Respiratory Chain Supercomplexes: From Structure to Function

Mitochondrial oxidative phospho rylation, the center of cellular metabolism, is pivotal for the energy production in eukaryotes. Mitochondrial oxidative phosphorylation relies on the mitochondrial respiratory chain, which consists of four main enzyme complexes and two mobile electron carriers. Mitochondrial enzyme complexes also assemble into respiratory chain supercomplexes (SCs) through specific interactions. The SCs not only have respiratory functions but also improve the efficiency of electron transfer and reduce the production of reactive oxygen species (ROS). Impaired assembly of SCs is closely related to various diseases, especially neurodegenerative diseases. Therefore, SCs play important roles in improving the efficiency of the mitochondrial respiratory chain, as well as maintaining the homeostasis of cellular metabolism. Here, we review the structure, assembly, and functions of SCs, as well as the relationship between mitochondrial SCs and diseases.     

基于迭代线性约束最小方差的稳健自适应脉冲压缩方法

针对常规自适应脉冲压缩方法在目标散射点与采样中心失配时旁瓣抑制性能下降的问题,该文提出一种基于迭代线性约束最小方差(RLCMV)的自适应脉冲压缩方法。该方法首先将自适应波束形成器算法引入到自适应脉冲压缩滤波器设计中。其次对目标及干扰单元进行线性约束,并用对角加载技术避免矩阵出现病态。最后构造了迭代运算方法,依次抑制不同大小目标的距离旁瓣。仿真结果表明,该算法可以有效抑制散射点随机分布目标的距离旁瓣,对散射点与采样中心失配情况具有较好的稳健性,在多目标及距离扩展目标场景中达到较好的旁瓣抑制性能,并在一定程度上提高了多普勒容性。     

非开挖拖管技术在电缆保护管施工中的应用

在城市道路地下电缆保护管下施工中,非开挖拖管施工技术是地下电缆保护管施工的一种比较新型的施工方法.以玉溪市棚户区改造玉交集团10 kV永久性供电工程中电缆保护管在穿越龙马路施工时采用的非开挖拖管技术为例,浅谈该技术在本项目中的应用.     

探测激光超声用的激光外差干涉仪

述了用外差干涉法探测固体表面激光超声的原理和实验结果,讨论了信号光、参考光的准直角对信噪比S/N的影响和信号光在分束器上的入射角对外差信号强度的影响。理论和实验结果表明:当选择人射角时,可以得到约2倍的外差信号强度。为提高检测信号的强度,提出并讨论了外差干涉的共轭输出法。     

二次熔盐锂电池用二硫化铁的合成及性能测试

利用加压水溶液法合成了黄铁矿型二硫化铁,研究了反应压力和溶液pH对产物中二硫化铁含量的影响,给出了合成反应的可能机理,测定了二硫化铁活性材料的利用率。     

环境友好催化剂催化合成硬脂酸正丁酯的一些方法

评述了氨基磺酸、离子交换树脂、十二水硫酸铁铵、硫酸钛、一水硫酸氢钠和固体超强酸催化合成硬脂酸正丁酯的方法。结果表明,它们是合成硬脂酸正丁酯的良好环境友好催化剂。