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11.
A 50-year-old woman ASA 2 underwent carbon dioxide hysteroscopy under general anaesthesia. Monitoring showed a sudden and rapid fall in end-tidal carbon dioxide followed by oxygen desaturation. She became pulseless and cyanosed. Resuscitation with oxygen, intravenous adrenaline and head-down tilt restored her to haemodynamic stability. Hyperbaric therapy was also administered as air embolism could not be excluded. 相似文献
12.
Cumulative step-size adaptation (CSA) based on path length control is regarded as a robust alternative to the standard mutative self-adaptation technique in evolution strategies (ES), guaranteeing an almost optimal control of the mutation operator. This paper shows that the underlying basic assumption in CSA--the perpendicularity of expected consecutive steps--does not necessarily guarantee optimal progress performance for (mu/mu(I), lambda) intermediate recombinative ES. 相似文献
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Carina A. Lämmle Adam Varady Thorsten G. Müller Dr. Caterina Sturtzel Michael Riepl Bettina Mathes Jenny Eichhorst Dr. Anje Sporbert Dr. Martin Lehmann Prof. Dr. Hans-Georg Kräusslich Dr. Martin Distel Dr. Johannes Broichhagen 《Chembiochem : a European journal of chemical biology》2021,22(3):548-556
Selective targeting of DNA by means of fluorescent labeling has become a mainstay in the life sciences. While genetic engineering serves as a powerful technique and allows the visualization of nucleic acid by using DNA-targeting fluorescent fusion proteins in a cell-type- and subcellular-specific manner, it relies on the introduction of foreign genes. On the other hand, DNA-binding small fluorescent molecules can be used without genetic engineering, but they are not spatially restricted. Herein, we report a photocaged version of the DNA dye Hoechst33342 (pcHoechst), which can be uncaged by using UV to blue light for the selective staining of chromosomal DNA in subnuclear regions of live cells. Expanding its application to a vertebrate model organism, we demonstrate uncaging in epithelial cells and short-term cell tracking in vivo in zebrafish. We envision pcHoechst as a valuable tool for targeting and interrogating DNA with precise spatiotemporal resolution in living cells and wild-type organisms. 相似文献
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Albert Albers Tobias Deigendesch Hans-Georg Enkler Stefan Hauser Pablo Leslabay Jochen Oerding 《Microsystem Technologies》2008,14(12):1781-1787
Miniaturization of macroscopic mechanical systems enables the opening of new areas of application for micro technological
systems. Because of technological restrictions, especially when applying miniaturized conventional manufacturing techniques,
shape and material deviations cannot be scaled down in the same dimension like micro parts. Thus, the long-term objective
is to ensure the functioning by appropriate design measures. In doing so, determination of the transfer behavior by modeling
and simulation is required. This work presents two ways for ensuring the required properties of micro gears and describes
how the results do correlate. The experimental way uses the radial composite inspection as it is used in the macroscopic world.
The simulative way deploys a rolling simulation by utilization of finite element analysis. The virtual prototypes are originated
on measured real test gears. When comparing experiment and simulation of the rolling behavior, in some short and long wave
areas deviations can be recognized. These can be ascribed to the reduction from three to two dimensions when modeling. Other
deviations might be based on inaccuracies when mounting into the test rig. In other areas quite good correlations of test
and simulation could be ascertained. 相似文献
17.
Die durch Walzen oder Schmieden im Bereich von 700 bis 1050°C thermomechanisch behandelten Manganhartstähle X 120 Mn 12 und X 120 MnCrSiV 12 werden unter Einbeziehung einer anschließenden Haltedauer bei Umformtemperatur bzw. bei 440°C bezüglich ihres Gefüges und ihrer Eigenschaften vergleichend untersucht. Es zeigt sich, daß die Legierungszusätze an Chrom, Silicium und Vanadin eine höhere Umformtemperatur zur Erzielung einer gewünschten Verfestigung zulassen und damit die thermomechanische Behandlung erleichtern. Der Verschleißwiderstand wird nicht beeinträchtigt. 相似文献
18.
Bryan D. Paulsen Ruiheng Wu Christopher J. Takacs Hans-Georg Steinrück Joseph Strzalka Qingteng Zhang Michael F. Toney Jonathan Rivnay 《Advanced materials (Deerfield Beach, Fla.)》2020,32(40):2003404
The structure and packing of organic mixed ionic–electronic conductors have an especially significant effect on transport properties. In operating devices, this structure is not fixed but is responsive to changes in electrochemical potential, ion intercalation, and solvent swelling. Toward this end, the steady-state and transient structure of the model organic mixed conductor, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), is characterized using multimodal time-resolved operando techniques. Steady-state operando X-ray scattering reveals a doping-induced lamellar expansion of 1.6 Å followed by 0.4 Å relaxation at high doping levels. Time-resolved operando X-ray scattering reveals asymmetric rates of lamellar structural change during doping and dedoping that do not directly depend on potential or charging transients. Time-resolved spectroscopy establishes a link between structural transients and the complex kinetics of electronic charge carrier subpopulations, in particular the polaron–bipolaron equilibrium. These findings provide insight into the factors limiting the response time of organic mixed-conductor-based devices, and present the first real-time observation of the structural changes during doping and dedoping of a conjugated polymer system via X-ray scattering. 相似文献
19.
We investigate the effect of the geometry of granular heaps on the pressure distribution. For given pressure distributions under cones we compute the pressure distribution under wedges using linear superposition. For cones with a pressure minimum, the pressure minimum for the corresponding wedge vanishes. Comparisons with experimental data gives good qualitative aggreement, but the total pressure is overestimated. 相似文献
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