Concentration of DNA in a flowing stream for high-sensitivity capillary electrophoresis

A novel sample pretreatment device is described, and its application to the concentration and purification of crude DNA samples in a flowing stream for subsequent capillary electrophoresis is demonstrated. The device consists of two gap junctions, each covered with a conductive membrane material and built upon a flow channel made of PEEK tubing. Upon the application of an electric field between the junctions, the negatively charged DNA fragments can resist the hydrodynamic flow stream and are trapped between the junctions. DNA fragments dissolved in microliter volumes are captured in a nanoliter-sized band by simply pushing the sample solution through the device. Depending on their electrophoretic mobility, other interfering materials in a crude sample can be removed from the trapped DNA fragments by washing. The selective permeability of the membrane to small ions allows efficient desalting. The concentrated and purified DNA fragments are released by simply turning off or reversing the electric field. Recovery is up to 95%. Performance of the device was evaluated using crude products of fluorescent dye-primer cycle-sequencing reactions. Compared to these crude reaction products, samples purified in the capture device and subsequently collected showed dramatically enhanced signal and resolution when run on a conventional capillary-electrophoresis instrument. Furthermore, the device could be connected in-line to a capillary system for direct injection. The device has great potential for enabling lab-on-a-chip systems to be used with real-world samples.     

Fluidic preconcentrator device for capillary electrophoresis of proteins

A new preconcentration device was developed for analysis of proteins by capillary electrophoresis (CE). The microfluidic device uses an electric field to capture proteins that pass through the system. The capture zone is maintained in the flow stream by the interaction between hydrodynamic and electrical forces. The device consists of a flow channel made of PEEK tubing with two electrical junctions, each of which is covered with a conductive membrane. A syringe pump provides the flow stream and also allows the injection of up to 13.5 microL of a dilute sample. The system can be easily connected to a CE device postcapture for off-line preconcentration of proteins. For the proteins used in this study, preconcentration factors up to 40-fold can be achieved. CE detection limits for bovine carbonic anhydrase, alpha-lactalbumin and beta-lactoglobulins A and B were in the nanomolar range using UV detection at 200 nm. Preconcentration is dependent on both time and initial protein concentration. We show the possibility of using an off-line fluidic preconcentrator device employing counterflow capillary electrophoresis with minimum sample manipulation, achieving detection limits similar to on-line approaches.     

Holocaust trauma reconstructed: Individual, familial, and social trauma.

Psychic trauma is characterized by ego damage, regression, and relative helplessness. Following a historic catastrophe, psychic trauma with ego damage is frequently encountered as cumulative trauma. Reconstruction was essential to the understanding of an adult patient initially unaware of his holocaust trauma. When the patient was a child, he and his parents were simultaneously traumatized. Intrapsychic intergenerational psychosocial and neurobiological factors were adverse codeterminants of his cumulative trauma. Isolated affects and fragmented memories required sequential connection in the context of family, identity, and society. Reconstruction importantly contributed to ego reintegration of a lost, lacerated childhood, and to adaptive mastery of trauma and conflict. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A systems analysis of the energy environment in buildings

The author has derived a flow diagram of fire development in order to point out areas where current technology can be applied to the fire problem as well as areas where further research is required.     

Development of antipredator responses in snakes: II. Effects of recent feeding on defensive behaviors of juvenile garter snakes (Thamnophis sirtalis).

The effect of having eaten a large meal (50% of body weight) on the defensive behavior of juvenile garter snakes (Thamnophis sirtalis) was investigated. Twenty-five 10-wk-old snakes were tested for antipredator responses (strikes) directed toward a threatening stimulus (moving and nonmoving human hand) before and after having eaten a large worm segment. Snakes directed significantly (p?     

Central bottleneck influences on the processing stages of word production.

Does producing a word slow performance of a concurrent, unrelated task? In 2 experiments, 108 participants named pictures and discriminated tones. In Experiment 1, pictures were named after cloze sentences; the durations of the word-production stages of lemma and phonological word-form selection were manipulated with high- and low-constraint cloze sentences and high- and low-frequency-name pictures, respectively. In Experiment 2, pictures were presented with simultaneous distractor words; the durations of lemma and phoneme selection were manipulated with conceptually and phonologically related distractors. All manipulations, except the phoneme-selection manipulation, delayed tone-discrimination responses as much as picture-naming responses. These results suggest that early word-production stages-lemma and phonological word-form selection-are subject to a central processing bottleneck, whereas the later stage-phoneme selection-is not. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Understanding action beyond imitation: Reversed compatibility effects of action observation in imitation and joint action.

A robust finding in imitation literature is that people perform their actions more readily if they are congruent with the behavior of another person. These action congruency effects are typically explained by the idea that the observation of someone else acting automatically activates our motor system in a directly matching way. In the present study action congruency effects were investigated between an imitation task and a complementary action task. Subjects imitated or complemented a virtual actor's grasp on a manipulandum. In both tasks, a color-cue could be presented forcing subjects to ignore the task rule and execute a predefined grasp. Reaction times revealed a reversal of congruency effects in the complementary action task, suggesting that subjects were able to circumvent the automatic tendency to copy actions or postures of another person. In 2 additional control experiments, congruency effects were replicated, and a Simon effect was identified to underlie faster responses in the imitation task. These results make a case against current theoretical views on imitation and direct matching in favor of more flexible models of perception-action coupling. (PsycINFO Database Record (c) 2010 APA, all rights reserved)