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排序方式: 共有156条查询结果,搜索用时 11 毫秒
31.
Fukuyama Y Yasuda N Kim J Murayama H Ohshima T Tanaka Y Kimura S Kamioka H Moritomo Y Toriumi K Tanaka H Kato K Ishikawa T Takata M 《The Review of scientific instruments》2008,79(4):045107
An ultra-high-precision clock system for long time delay has been developed for picosecond time-resolved x-ray diffraction measurements using synchrotron radiation (SR) pulses and synchronized femtosecond laser pulses. The time delay control between pump laser pulse and the probe SR pulse was achieved by combining an in-phase quadrature modulator and a synchronous counter. This method allowed us to change the delay time by a nearly infinite amount while maintaining the precision of +/-8.40 ps. Time-resolved diffraction measurements using the delay control system were demonstrated for precise measurement of an acoustic velocity in a single crystal of gallium arsenide. 相似文献
32.
Fluorescent imaging of endothelial glycocalyx layer with wheat germ agglutinin using intravital microscopy
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Hanae Kataoka Akira Ushiyama Hayato Kawakami Yoshihiro Akimoto Sachie Matsubara Takehiko Iijima 《Microscopy research and technique》2016,79(1):31-37
Endothelial glycocalyx (GCX) is located on the apical surface of vascular endothelial cells and is composed of a negatively‐charged network of proteoglycans and glycoproteins. The GCX plays an important role in maintaining the integrity of vascular walls and preventing leakage of plasma. Therefore, degradation of the GCX is believed to lead to pathological leakage of plasma. Because the GCX is a very thin layer, its ultrastructural image has been demonstrated on electron microscope. To explore the function of the GCX, it should be visualized by a microscope in vivo. Thus, we developed in vivo visualization technique of the GCX under fluorescence microscopy using a mouse dorsal skinfold chamber (DSC) model. To label and visualize the GCX, we used fluorescein isothiocyanate (FITC)‐labeled lectin, which has a high specificity for sugar moieties. We examined the affinity of the different lectins to epivascular regions under an intravital fluorescent microscope. Among seven different lectins we examined, FITC labeled Triticum vulgaris (wheat germ) agglutinin (WGA) delineated the GCX most clearly. Binding of WGA to the GCX was inhibited by chitin hydrolysate, which contained WGA‐binding polysaccharide chains. Furthermore, the septic condition attenuated this structure, suggesting structural degradation of endothelial GCX layer. In conclusion, FITC‐labeled WGA lectin enabled visualization of endothelial GCX under in vivo fluorescence microscopy. Microsc. Res. Tech. 79:31–37, 2016. © 2015 Wiley Periodicals, Inc. 相似文献
33.
Dietary effects of medium-chain triacylglycerols (MCT) and fucoxanthin (Fc) on abdominal fat weight were determined using KK-Ay obese mouse. Experimental diet contained MCT(0.9%), Fc (0.1%), or MCT (0.9%) +Fc (0.1%). The abdominal fat weight of mice fed with Fc was significantly lower than that of mice fed with MCT. Uncoupling protein 1 (UCP1), a key molecule for metabolic thermogenesis, was clearly expressed in the white adipose tissue (WAT) of mice fed Fc, but little expression in that of the mice fed MCT. The anti-obesity effect of Fc was increased by mixing Fc with MCT. This increase would be due to the increase in the absorption rate of Fc by MCT. 相似文献
34.
35.
Multifaceted Analysis of IL-23A- and/or EBI3-Including Cytokines Produced by Psoriatic Keratinocytes
Kota Tachibana Nina Tang Hitoshi Urakami Ai Kajita Mina Kobashi Hayato Nomura Minori Sasakura Satoru Sugihara Fan Jiang Nahoko Tomonobu Masakiyo Sakaguchi Mamoru Ouchida Shin Morizane 《International journal of molecular sciences》2021,22(23)
Interleukin (IL) 23 (p19/p40) plays a critical role in the pathogenesis of psoriasis and is upregulated in psoriasis skin lesions. In clinical practice, anti-IL-23Ap19 antibodies are highly effective against psoriasis. IL-39 (p19/ Epstein-Barr virus-induced (EBI) 3), a newly discovered cytokine in 2015, shares the p19 subunit with IL-23. Anti-IL-23Ap19 antibodies may bind to IL-39; also, the cytokine may contribute to the pathogenesis of psoriasis. To investigate IL23Ap19- and/or EBI3-including cytokines in psoriatic keratinocytes, we analyzed IL-23Ap19 and EBI3 expressions in psoriasis skin lesions, using immunohistochemistry and normal human epidermal keratinocytes (NHEKs) stimulated with inflammatory cytokines, using quantitative real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and liquid chromatography-electrospray tandem mass spectrometry (LC-Ms/Ms). Immunohistochemical analysis showed that IL-23Ap19 and EBI3 expressions were upregulated in the psoriasis skin lesions. In vitro, these expressions were synergistically induced by the triple combination of tumor necrosis factor (TNF)-α, IL-17A, and interferon (IFN)-γ, and suppressed by dexamethasone, vitamin D3, and acitretin. In ELISA and LC-Ms/Ms analyses, keratinocyte-derived IL-23Ap19 and EBI3, but not heterodimeric forms, were detected with humanized anti-IL-23Ap19 monoclonal antibodies, tildrakizumab, and anti-EBI3 antibodies, respectively. Psoriatic keratinocytes may express IL-23Ap19 and EBI3 proteins in a monomer or homopolymer, such as homodimer or homotrimer. 相似文献
36.
Tohmyoh H Saka M Hirayanma H 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2006,53(12):2481-2483
The potential of the water-immersion and dry-contact acoustic imaging techniques for detecting nanometer gaps embedded in silicon is studied. The sensitivity for detecting gaps of over 10 nm in height is governed only by the lateral resolution of the imaging and is independent of the height of the gap. 相似文献
37.
Techniques for patterned modification of substrate surfaces are important for the formation of microarrays on protein chips. One strategy is based on partial plasma polymerization to create protein adhesive/non-adhesive regions of several tens of micrometers in size. Protein immobilization on a plasma functionalized surface occurs by physical adsorption of a protein solution. Distinct 80 × 80 μm2 square spots of fluorescently labeled protein, immunoglobulin G, surrounded by a non-fluorescent 80 μm wide grid were observed. The monomer tetraethylene glycol diethyl ether was the best candidate for plasma polymerization to produce a protein-repellent surface. However, the choice of monomer for the protein adhesive surface was strongly dependent on the type of protein. Binding assays were performed by protein immobilization on the patterned substrate and subsequent reaction with fluorescently labeled counterpart proteins (secondary antibodies). Fluorescent patterning similar to the original pattern was observed. In contrast, patterning was not observed when a fluorescently labeled non-counterpart protein was reacted with the surface. This indicated that the proteins were selectively adsorbed onto the target patterned surface and retained their biofunctional activity in addition to having a suitable orientation of the molecule. Moreover, the protein non-adhesive layer plays a role for suppression of the background signal and enhancement of the signal to noise (S/N) ratio. The proposed technique provides a simple and robust method for protein patterning. 相似文献
38.
Dietary Tuna Dark Muscle Protein Attenuates Hepatic Steatosis and Increases Serum High‐Density Lipoprotein Cholesterol in Obese Type‐2 Diabetic/Obese KK‐Ay Mice
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Hayato Maeda Ryota Hosomi Mari Fukuda Yuki Ikeda Munehiro Yoshida Kenji Fukunaga 《Journal of food science》2017,82(5):1231-1238
Tuna muscle consists of light and dark muscle in approximately equal proportions. However, besides for the light muscle of tuna, cod, sardine, and salmon, few researches have assessed the health‐promoting functions of fish protein. Therefore, we evaluated the mechanisms underlying the alteration of lipid storage and cholesterol metabolism following the intake of tuna dark muscle protein (TDMP) by obese type‐2 diabetic/obese mice. Four‐week‐old male KK‐Ay mice were separated into 2 dietary groups, with one group receiving a casein‐based diet and the other receiving a diet with the substitution of part of the protein (50%, w/w) by TDMP (TDMP diet) for 4 wk. The TDMP diet significantly increased the content of serum high‐density lipoprotein cholesterol, partly due to the reduction of the expression of scavenger receptor class B member 1 in epididymal white adipose tissue. In addition, dietary TDMP decreased the content of hepatic triacylglycerol, which could be due to the enhancement of carnitine palmitoyltransferase‐2 activity through the activation of the expression of the peroxisome proliferative activated receptor‐α in the liver. These results suggest that TDMP could have the potential to prevent the development of obesity‐related diseases by suppressing the storage of hepatic triacylglycerol and cholesterol. 相似文献
39.
Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis,and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor
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40.
Yanagida H Matsuura T Kazuta Y Yomo T 《Chembiochem : a European journal of chemical biology》2011,12(6):962-969
There is a great deal of interest in proteins that can bind covalently to target molecules, as they allow unambiguous experiments by tight binding to molecules of interest. Here, we report the generation of proteins that undergo covalent labeling with small molecules through in vitro selection by using ribosome display. Selection was performed from a mutant library of the WW domain with a biotinylated peptide as its binding target, in which the biotin and the peptide are connected by a disulfide bond. After five rounds of selection, we identified mutants carrying a particular cysteine mutation. The binding target reacted specifically with the selected mutant, even in the presence of other proteins, and resulted in the generation of biotin- or peptide-labeled WW domains by thiol-disulfide exchange. When the mutant was fused to a protein of interest, the fusion protein was also labeled with biotin. Thus, the characteristics of the selected mutant should be suitable as a tag sequence that can be covalently labeled with small synthetic molecules. These results indicate that the rapid and efficient generation of such proteins is possible by ribosome display. 相似文献