3D-Druck für Hochvakuumanwendungen

To mark our anniversary, we are presenting articles that have been particularly appreciated by readers of our online edition once again in print. Here: 3D-Printing for High Vacuum Applications 3D printing technology has made the leap from a home-based private practice to industrial manufacturing. Due to the increasing reliability of printers and increasing material diversity, especially in the metal sector, double-digit percentage growth rates are possible in the coming years. This thesis deals with the manufacture of parts made by 3D printing for high vacuum application. Different components are printed and examined for their vacuum compatibility. As shown furthermore, conventionally made standard components can be vacuum sealed to printed parts, which enables cost-effective production of more complex components, such as e.g. a vacuum chamber allows. In addition, functional components can already be realized in the manufacturing process. The integration of a system of flow channels directly into the wall of a chamber is just one example. Thus, such a chamber can be heated during evacuation and effectively cool in later operation.     

Cardiovascular function and brain metabolites in normal weight and intrauterine growth restricted newborn piglets--effect of mild hypoxia

In order to clarify the influence of intrauterine growth restriction on systemic hemodynamics, catecholamine response, and regional distribution of brain energy metabolites per se and during mild hypoxic episodes a study was performed in thirty newborns with a well-characterized state of intrauterine and intra-natal development. Thirty animals were divided into fifteen normal weight piglets (NW) and fifteen intrauterine growth restricted (IUGR) piglets according to their birth weight. Category "NW" covered animals with a birth weight of > 40th percentile; IUGR category covered animals with a birth weight of > 5th and < 10th percentiles. Animals were anesthetized with halothane in 70% nitrous oxide and 30% oxygen and after immobilization artificially ventilated. The acid-base balance and blood gas values at baseline conditions were similar within the different groups investigated and consistent with other data obtained from anesthetized and artificially ventilated newborn piglets. Mild hypoxic hypoxia which was induced by lowering the FiO2 from 0.35 to 0.15 resulted in reduced arterial pO2 (NW: from 115 +/- 37 mmHg to 39 +/- 7 mmHg; IUGR: from 117 +/- 23 mmHg to 39 +/- 3 mmHg; p < 0.05), but arterial pH and pCO2 remained unchanged. Under baseline conditions arterial blood pressure, cardiac output, and myocardial contractility, expressed as dp/dt(max) and plasma catecholamine values were similar in all groups studied. Heart rate was slightly increased in IUGR (p < 0.05). Mild hypoxia led to a strong increase of myocardial contractility in NW as well as IUGR piglets to 2.4 and 2.7 fold and remained increased during recovery (p < 0.05). Moreover, total peripheral resistance was enhanced at the end of recovery period in IUGR animals (p < 0.05). There was a significant increase of epinephrine (E) in NW animals in comparison to sham-operated animals (p < 0.05). Interestingly, during reoxygenation the further increase in E and norepinephrine (NE) levels were enhanced in the animals which suffered from mild hypoxia (p < 0.05). Regional distribution of brain tissue metabolites was partly affected by intrauterine growth restriction. In particular, brain tissue glucose content was strongly reduced by 65 to 72 per cent in all brain regions investigated. Mild hypoxia led to an increase of about 30 percent in NW animals (p < 0.05). In IUGR piglets the percentage increase of brain glucose content was on an average more pronounced but with considerably higher variance. Also, a strong increase of brain lactate content appeared here (p < 0.05). In contrast, brain tissue ATP was quite similar in all groups studied, but brain creatine phosphate was significantly reduced in some forebrain structures of IUGR piglets after mild hypoxia (figure 2, p < 0.05). In summary, this investigation provides information on cardiovascular functions and brain metabolites of normal weight and naturally occurring growth restricted newborn piglets. Mild hypoxemia was well-tolerated from both animal groups. It is suggested that lactate may play a significant role as a source for brain energy production in the newborn IUGR piglets.     

Formal testing for separation assurance

In order to address the rapidly increasing load of air traffic operations, innovative algorithms and software systems must be developed for the next generation air traffic control. Extensive verification of such novel algorithms is key for their adoption by industry. Separation assurance algorithms aim at predicting if two aircraft will get closer to each other than a minimum safe distance; if loss of separation is predicted, they also propose a change of course for the aircraft to resolve this potential conflict. In this paper, we report on our work towards developing an advanced testing framework for separation assurance. Our framework supports automated test case generation and testing, and defines test oracles that capture algorithm requirements. We discuss three different approaches to test-case generation, their application to a separation assurance prototype, and their respective strengths and weaknesses. We also present an approach for statistical analysis of the large numbers of test results obtained from our framework.     

On pseudo maximum likelihood estimation for multivariate time series models with conditional heteroskedasticity

We consider a general multivariate conditional heteroskedastic model under a conditional distribution that is not necessarily normal. This model contains autoregressive conditional heteroskedastic (ARCH) models as a special class. We use the pseudo maximum likelihood estimation method and derive a new estimator of the asymptotic variance matrix for the pseudo maximum likelihood estimator. We also study four special cases in this class, which are conditional heteroskedastic autoregressive moving-average models, regression models with ARCH errors, models with constant conditional correlations, and ARCH in mean models.     

Influence of the measurement conditions on the sensitivity of SnO2 gas sensors operated thermo-cyclically

The conductance of several tin oxide gas sensitive layers was simultaneously measured in thermo-cyclic and isothermal operation mode at various concentrations of CO and propene in air, respectively. Different measurement conditions were set by the cycle time, the gas flow rate, the humidity and the thickness of the sensitive layer. The sensor response is expressed by the conductance-over-time profiles (CTPs), the gas sensitivity or by the sum of the CTP sampling points. The resultant sensitivities from the CTPs were found to be higher than those of the isothermal measurements. The CTPs considerably change with the kind of gas as well as with the variation of the measurement conditions. This is discussed for both types of target gases with respect to the temperature, the adsorbates assumed and the reaction kinetics in relation to the gas transport conditions in the porous gas sensitive layer.     

Detection of colorectal adenoma and cancer based on transthyretin and C3a-desArg serum levels

Colorectal cancer is the second leading cause of cancer death, and it develops from benign colorectal adenomas in over 95% of patients. Early detection of these cancer precursors by screening tests and their removal can potentially eradicate more than 95% of colorectal cancers before they develop. To discover sensitive and specific biomarkers for improvement of pre‐clinical diagnosis of colorectal adenoma and cancer, we analysed in two independent studies (n = 87 and n = 83 patients) serum samples from colorectal cancer (stage III), colorectal adenoma and control patients using SELDI‐TOF‐MS. Extensive statistical analysis was performed to establish homogeneous patient groups based on their clinical data. Two biomarkers that were each able to distinguish control patients from either colorectal adenoma or colorectal cancer patients (p<0.001) were identified as transthyretin (pre‐albumin) and C3a‐desArg by MS/MS and were further validated by antibody‐based assays (radial immunodiffusion, ELISA). A combination of both proteins clearly indicated the presence of colorectal adenoma or carcinoma. Using a cut‐off of <0.225 g/L for transthyretin and >1974 ng/mL for C3a‐desArg, we found a sensitivity and specificity for colorectal adenoma of 96% and 70%, respectively.     

Configuration Maintenance for Distributed Applications Management

The MANDAS project has defined a layered architecture for the management of distributed applications. In this paper we examine a vertical slice of this architecture, namely the management applications and services related to configuration management. We introduce an information model which captures the configuration information for distributed applications and discuss a repository service based on the model. We define a set of services and management applications to support maintenance of configuration information, and describe how the different types of configuration information are collected. Finally, we present two management applications that use configuration information.     

S-phase fractions of breast carcinomas. Relationships to morphology, estrogen and progesterone receptors, and early recurrence

Analysis of the S-phase fractions (SPF) measured by in vitro thymidine labeling, morphological appearances, and estrogen receptor (ER) assays of primary invasive breast carcinomas demonstrated several interrelationships. Lobular, mucinous, tubular, and adenocystic carcinomas consistently had low SPF and were usually positive for ER. The same was true for the carcinomas of no special histologic type [the not otherwise specified (NOS) group of E. R. Fisher including "infiltrating ductal" and undifferentiated carcinomas] with minimal anaplasia. Medullary, atypical medullary, and morphologically unclassifiable carcinomas with marked nuclear anaplasia nearly always had high SPF and were usually negative for ER. High SPF was associated with advanced stages of carcinoma initially or with early recurrence following mastectomy.