Vulnerability to aging in the rat serotonergic system

Distributional changes of serotonergic fibers associated with aging were demonstrated immunohistochemically. Old rat brains were morphologically characterized by the presence of peculiar features of serotonergic fibers not found in the young adult brain. In 24-month-old rats, these aberrant serotonergic fibers were subdivided into two groups according to morphological alterations: type 1 fibers consisting of thin fibers with moderately enlarged varicosities, and type 2 fibers consisting of much thicker fibers that have even larger varicosities and a tortuous course. These two types of fibers were distributed differentially in the forebrain. Type 1 fibers were found mainly in the striatum and frontoparietal cortex, whereas type 2 fibers were found in the posterior cingulate cortex and dentate gyrus of the hippocampal formation. Both types of aberrant fibers were seen in amygdala, frontoparietal cortex, hypothalamus, and thalamus. In 36-month-old rats, more highly degenerating arborizations were detected, and these aberrant ramifications were classified as follows based on shape as: type 3 fibers consisting of highly arborized thin fibers with a larger number of larger varicosities, and type 4 fibers consisting of thick fibers with abundant larger varicosities. Distributional difference indicated that type 1 fibers progress into type 3 fibers, whereas type 2 develop into type 4 fibers. These findings suggest the possibility that one set of pathological fibers emanate from the dorsal raphe nucleus and the other from the median raphe. Moreover, both two sets of serotonergic fibers show age-related aberrations in their morphology over same time course.     

Local secretion of corticotropin-releasing hormone by enterochromaffin cells in human colon

BACKGROUND/AIMS: Corticotropin-releasing hormone (CRH) is a regulator of the hypothalamic-pituitary-adrenal axis and a coordinator of the gastrointestinal response to stress. In addition to its central effects, CRH has peripheral effects on the immune system. CRH is present in several human tissues, such as the brain, spinal cord, adrenal medulla, lung, liver, peripheral blood leukocytes, as well as the gastrointestinal tract. The current study examined the local production of CRH in the normal human colon. METHODS: Normal human colonic tissues obtained by endoscopic biopsy were immunostained with anti-CRH and anti-5-hydroxytryptamine antibody and analyzed for CRH messenger (m)RNA by a reverse-transcribed polymerase chain reaction method and by in situ hybridization. RESULTS: Immunoreactive CRH and CRH mRNA were detected in the colonic mucosal cells in the neighborhood of the base of the crypts. The mucosal cells that expressed CRH mRNA also immunostained with anti-5-hydroxytryptamine antibody. CONCLUSIONS: Normal human colonic mucosal enterochromaffin cells produce CRH. CRH in the colonic mucosa may play a role in the modulation of the intestinal immune system and/or other gastrointestinal functions basally during stressful conditions.     

Interaction between Ca2+, K+, carbamazepine and zonisamide on hippocampal extracellular glutamate monitored with a microdialysis electrode

1. Multiple components of hippocampal glutamate release were examined by study of Ca2+- and K+-evoked hippocampal extracellular glutamate release using an in vivo microdialysis glutamate biosensor in urethane-anaesthetized rats. In addition, the effects of the antiepileptic drugs, carbamazepine (CBZ) and zonisamide (ZNS) perfused through the probe on glutamate release were assessed. 2. Basal glutamate levels were below detection limits (approximately 0.1 microM). An increase in extracellular KCl (from 2.7 to 50 and 100 mM) increased extracellular hippocampal glutamate levels to 9.2+/-1.4 and 20.0+/-2.6 microM, respectively, calculated from the area under curve (AUC) for 60 min. 3. This KCl-evoked glutamate release consisted of three components: an initial transient rise, a late gentle rise, and late multiple phasic transient rises. 4. An increase in or removal of extracellular CaCl2 levels respectively enhanced and reduced the 50 mM KCl-evoked hippocampal glutamate release (AUC for 60 min) from 9.2+/-1.4 to 12.4+/-2.1 and 5.8+/-0.9 microM. 5. Perfusion with 100 microM CBZ or 1 mM ZNS inhibited both the 50 mM KCl-evoked hippocampal glutamate release (AUC for 60 min) from 9.2+/-1.4 to 5.5+/-1.1 and to 5.8+/-1.3 microM, respectively, as well as the stimulatory effects of Ca2+ on KCl-evoked hippocampal glutamate release. 6. These results suggest that both CBZ and ZNS may reduce epileptiform events by inhibiting excitatory glutamatergic transmission.     

Estimating power-frequency characteristics in power systems by means of spectral analysis techniques

This paper shows that normal power system operating data may be used to determine the power-frequency characteristics in power systems. Data are processed as random signals using spectral analysis techniques, and the results estimate the generator and load power-frequency characteristics as transfer functions. The generator characteristics K_g are determined as a transfer function of the system in which input signals are frequency fluctuation ΔF and generator power setting signal ΔP_g, and output signal is generator power output ΔP_e, To estimate the load characteristics K_l, a difficulty arises. There is a relation ΔP_/ = ΔL_o + K_lΔF between actual load ΔP_l, ideal load under the state of nominal frequency ΔL_o, and fluctuation of system frequency ΔF. Because it is impossible to measure ΔL_o directly, ΔL_o and K_lΔF cannot be separated. With regard to a partial system, when its capacity is small enough compared with the total system capacity, the contribution of ΔL_o in a partial system to ΔF of the total system may be ignored, that is, ΔF and ΔL_o are considered as independent phenomena. Thus the cross spectrum of ΔF and ΔL_o decreases to zero according to the increase of data in number, and K_l of the partial system can be estimated.     

Characterization of the hemorrhagic reaction caused by Vibrio vulnificus metalloprotease, a member of the thermolysin family

Vibrio vulnificus is an opportunistic human pathogen causing wound infections and septicemia, characterized by hemorrhagic and edematous damage to the skin. This human pathogen secretes a metalloprotease (V. vulnificus protease [VVP]) as an important virulence determinant. When several bacterial metalloproteases including VVP were injected intradermally into dorsal skin, VVP showed the greatest hemorrhagic activity. The level of the in vivo hemorrhagic activity of the bacterial metalloproteases was significantly correlated with that of the in vitro proteolytic activity for the reconstituted basement membrane gel. Of two major basement membrane components (laminin and type IV collagen), only type IV collagen was easily digested by VVP. Additionally, the immunoglobulin G antibody against type IV collagen, but not against laminin, showed sufficient protection against the hemorrhagic reaction caused by VVP. Capillary vessels are known to be stabilized by binding of the basal surface of vascular endothelial cells to the basement membrane. Therefore, specific degradation of type IV collagen may cause destruction of the basement membrane, breakdown of capillary vessels, and leakage of blood components including erythrocytes.     

A splicing and inspection technique for single-mode fibers using direct core monitoring

A basic study has been made of single-mode fiber splicing techniques using direct core monitoring, which includes a highly accurate axis alignment and a post-inspection technique to guarantee the splice-loss standards. The standard conformance reliability, taking into account the core detection uncertainty, was studied by statistical theory. A method for setting acceptance criteria and necessary core detection accuracy has been established. Splice loss due to parameter mismatch, which cannot be estimated by core monitoring, was also examined.     

Temperature dependence of gate forward turn-on voltage (Vf) of i-Al0.3Ga0.7As/n-GaAs HIGFET's

Forward gate current-voltage characteristics and their temperature dependence are investigated for i-Al_0.3Ga_0.7As/n-GaAs doped-channel HIGFET's (DC-HIGFET's) with the gate length of 0.3 μm. The temperature coefficient of the gate forward turn-on voltage (Vf) varies with the thickness (t_U) of an i-AlGaAs layer, and shows a minimum value of -0.8~-0.9 mV/deg at t_u=10 nm     

Rhabdomyosarcoma in the inferior vena cava with secondary Budd-Chiari syndrome

Abdominoplasty is a common procedure in plastic surgery. Reviewing 150 patients who underwent abdominoplasty, it has been observed that 72% of the patients already had an abdominal scar. How to deal with abdominoplasty in an abdomen with a previous scar is discussed in this article.     

Three-dimensional optical memory with a photorefractive crystal

We propose a three-dimensional optical-memory device in which refractive dot data are recorded directly into a photorefractive crystal. To record a single bit of datum, one focuses a laser beam with an objective lens onto a specific spot in a crystal, thereby changing its refractive index locally as a result of photorefraction. To record in three dimensions, one keeps the objective lens stationary while the crystal is translated. The beam-spot intensity is modulated with a beam shutter according to the logic state of the data point. The recorded data points are read with a phase-contrast microscope objective lens. We present experimental results of three-dimensional recording and reading with a LiNbO(3) crystal. The distribution of the refractive index formed by a focused beam is also analyzed with the charge-transport model.