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71.
The correlation functions of the fluctuations of vibrational frequencies of azide ions and carbon monoxide in proteins are determined directly from stimulated photon echoes generated with femtosecond infrared pulses. The asymmetric stretching vibration of azide bound to carbonic anhydrase II exhibits a pronounced evolution of its vibrational frequency distribution on the time scale of a few picoseconds, which is attributed to modifications of the ligand structure through interactions with the nearby Thr-199. When azide is bound in hemoglobin, a more complex evolution of the protein structure is required to interchange the different ligand configurations, as evidenced by the much slower relaxation of the frequency distribution in this case. The time evolution of the distribution of frequencies of carbon monoxide bound in hemoglobin occurs on the approximately 10-ps time scale and is very nonexponential. The correlation functions of the frequency fluctuations determine the evolution of the protein structure local to the probe and the extent to which the probe can navigate those parts of the energy landscape where the structural configurations are able to modify the local potential energy function of the probe. 相似文献
72.
This paper reports two cases of neotropical echinococcosis caused by Echinococcus oligarthrus and E. vogeli, neither of which has been reported from Suriname. Case 1, a six-year-old boy, presented a 15 x 25 mm retro-ocular cystic tumor (observed by ultrasound, computed tomography scan, and magnetic resonance imaging) causing exophthalmia, chemosis, palpebral ptosis, and blindness of the left eye. Of two tentative diagnoses, Echinococcus cyst or dermoid tumor, the former was shown to be correct at surgery when a clear liquid and detached protoscoleces were aspirated. Rostellar hooks of the protoscolex were characteristic of E. oligarthrus. Case 2, a 41-year-old man, had polycystic masses excised from the liver and abdomen. A presurgery diagnosis of E. vogeli infection was made due to calcifications seen in the lesions, positive serology, residence of the patient in the tropical forest, and later by the size and shape of rostellar hooks. The presence of these two parasites in one of the former Guianas is not surprising; both species are endemic in tropical forest in Central and South America wherever people have not exterminated wild canids, especially the bush dog, (Speothos venaticus), and felids (wild cats of several species), along with pacas, agoutis, and other rodents that serve as intermediate host of these two cestodes. Eighty-six cases of polycystic echinococcosis are known in people from 11 countries from Nicaragua to Argentina: 32 due to E. vogeli, three to E. oligarthrus, and 51 for which determination of the species was not possible because the hooks of the protoscolex were not found or described. Research to elucidate aspects of transmission of E. vogeli and E. oligarthrus is of practical importance for defining measures for preventing the severe and frequently fatal illnesses caused by these two cestodes. 相似文献
73.
A micellar electrokinetic capillary chromatographic (MECC) method has been developed and optimised for the separation of 5-aminolaevulinic acid (ALA) and porphobilinogen (PBG). The running buffer consisted of a mixture of 20 mM sodium phosphate and 20 mM sodium borate containing 50 mM sodium dodecyl sulphate (SDS) adjusted to pH 9.5 with 1 M NaOH. The running voltage and temperature were 20-25 kV and 30 degrees C, respectively. The MECC method for the analysis of PBG is fast and simple and is useful for the screening of PBG in the urine of patients suspected to have acute intermittent porphyria (AIP), and for the confirmation of lead exposure by measuring red-cell ALA-dehydratase (ALA-D) activity with ALA as the enzyme substrate. 相似文献
74.
75.
Current applications of electronic performance monitoring based on job design theories that consider worker performance rather than stress issues are likely to generate unsatisfying and stressful jobs (Smith et al, 1986). This study examines critical job design elements that could influence worker stress responses in an electronic monitoring context. A questionnaire survey of employees in telecommunications companies representative of each region in the United States examined job stress in directory assistance, service representative and clerical jobs with specific emphasis on the influence of electronic monitoring of job performance, satisfaction and employee health. Useable surveys were received from 745 employees representing seven operating companies and AT & T; a response rate of about 25%. The results of this survey indicated that employees who had their performance electronically monitored perceived their working conditions as more stressful, and reported higher levels of job boredom, psychological tension, anxiety, depression, anger, health complaints and fatigue. It is postulated that these effects may be related to changes in job design due to electronic performance monitoring. 相似文献
76.
77.
Yeonsil Moon Changmok Lim Yeahoon Kim Won-Jin Moon 《International journal of molecular sciences》2021,22(6)
The role of the blood–brain barrier (BBB) breakdown has been recognized as being important in Alzheimer’s disease pathogenesis. We aimed to evaluate whether regional BBB integrity differed according to sex and whether differences in BBB integrity changed as a consequence of aging or cognitive decline, using dynamic contrast-enhanced (DCE)-magnetic resonance imaging (MRI). In total, 75 participants with normal cognition (NC) or mild cognitive impairment (MCI) underwent cognitive assessments and MRI examination including DCE-MRI. Regional Ktrans was calculated in cortical regions and the Patlak permeability model was used to calculate BBB permeability (Ktrans, min−1). Females had a lower median Ktrans in the cingulate and occipital cortices. In the “older old” group, sex differences in Ktrans were only observed in the occipital cortex. In the MCI group, sex differences in Ktrans were only observed in the occipital cortex. Age was the only predictor of cognitive assessment scores in the male MCI group; however, educational years and Ktrans in the occipital cortex could predict cognitive scores in the female MCI group. Our study revealed that females may have better BBB integrity in cingulate and occipital cortices. We also found that sex-related differences in BBB integrity are attenuated with aging or cognitive decline. 相似文献
78.
Kin Kit Fong Inn Shi Tan Henry Chee Yew Foo Man Kee Lam Adrian Chiong Yuh Tiong Steven Lim 《中国化学工程学报》2021,33(5):297-305
The rising production of produced water from oilfields had been proven to bring detrimental environmental effects. In this study, an efficient, recyclable, and environmental-friendly reduced graphene oxide immobilized κ-Carrageenan hydrogel composite(κCa GO) was fabricated as an alternative sorbent for crude oil-in-water demulsification. Polyethyleneimine(PEI) was employed to form a stable hydrogel composite. The conditions for the immobilization of graphene oxide(GO) on PEI-modified κ-Carrageenan(κC) beads were optimized appropriately. An immobilization yield of 77% was attained at 2% PEI, 2 h immobilization activation time, and p H6.5. Moreover, the synthesized κCa GO is capable of demulsification with an average demulsification efficiency of 70%. It was found that the demulsification efficiency increases with salinity and κCa GO dosage, and it deteriorates under alkaline condition. These phenomena can be attributed to the interfacial interactions between κCa GO and the emulsion. Furthermore, the κCa GO can be recycled to use for up to six cycles without significant leaching and degradation. As such, the synthesized κCa GO could be further developed as a potential sorbent substitute for the separation of crude oil from produced water. 相似文献
79.
80.
Jeung -Soo Huh Jae -Chang Kim Jeong -Ok Lim K. F. Jensen 《Metals and Materials International》1997,3(2):103-107
The growth of high quality ZnSe by organometallic vapor phase epitaxy (OMVPE) has been investigated fortertiary-butyl allyl selenide (tBASe), combined with dimethylzinc-triethylamine (DMZn : NEt3). Single crystalline ZnSe films were grown on GaAs at temperature as low as 350°C with a reasonable growth rate (~1 µm/h). Secondary ion mass spectrometry (SIMS) spectra show a negligible carbon incorporation in ZnSe films from tBASe even at high VI/II ratio, in contrast the carbon concentration of 1021 cm-3 in ZnSe films grown from diallyl selenide (DASe)and methylallylselenide (MASe). Good surface morphology, crystalline and interface quality of ZnSe on (001) GaAs are confirmed by scanning electron microscopy, double crystal diffractometry (DCD) and Rutherford backscattering spectrometry (RBS). Photoluminescence at 10K shows sharp near-band-edge excitonic spectra. 相似文献