Significance of Hypermethylation of Tumor-Suppressor Genes PTGER4 and ZNF43 at CpG Sites in the Prognosis of Colorectal Cancer

The status of DNA methylation in primary tumor tissue and adjacent tumor-free tissue is associated with the occurrence of aggressive colorectal cancer (CRC) and can aid personalized cancer treatments at early stages. Tumor tissue and matched adjacent nontumorous tissue were extracted from 208 patients with CRC, and the correlation between the methylation levels of PTGER4 and ZNF43 at certain CpG loci and the prognostic factors of CRC was determined using the MassARRAY System testing platform. The Wilcoxon signed-rank test, a Chi-square test, and McNemar’s test were used for group comparisons, and Kaplan–Meier curves and a log-rank test were used for prediction. The hypermethylation of PTGER4 at the CpG_4, CpG_5, CpG_15, and CpG_17 tumor tissue sites was strongly correlated with shorter recurrence-free survival (RFS), progression-free survival (PFS), and overall survival (OS) [hazard ratio (HR) = 3.26, 95% confidence interval (CI) = 1.38–7.73 for RFS, HR = 2.35 and 95% CI = 1.17–4.71 for PFS, HR = 4.32 and 95% CI = 1.8–10.5 for OS]. By contrast, RFS and PFS were significantly longer in the case of increased methylation of ZNF43 at the CpG_5 site of normal tissue [HR = 2.33, 95% CI = 1.07–5.08 for RFS, HR = 2.42 and 95% CI = 1.19–4.91 for PFS]. Aberrant methylation at specific CpG sites indicates tissue with aggressive behavior. Therefore, the differential methylation of PTGER4 and ZNF43 at specific loci can be employed for the prognosis of patients with CRC.     

浅议日本小学开放式教学空间的设计思想

文章简要回顾了日本小学教育的发展历史,并且根据日本小学教育的情况,分析了其教学空间设计的几个特点。     

基于Windows2000的开放式数控系统设计

介绍了一种在Windows2000环境下利用Visual C 6.0实现基于PC的数控系统(PCNC)的方法.论述了系统的硬、软件结构及其开发方法.该系统硬件采用了工控机和运动控制器相结合的结构;软件采用多线程编程.将该系统应用于车床,成功地实现了轴类零件的加工.     

Physapruin A Enhances DNA Damage and Inhibits DNA Repair to Suppress Oral Cancer Cell Proliferation

The selective antiproliferation to oral cancer cells of Physalis peruviana-derived physapruin A (PHA) is rarely reported. Either drug-induced apoptosis and DNA damage or DNA repair suppression may effectively inhibit cancer cell proliferation. This study examined the selective antiproliferation ability of PHA and explored detailed mechanisms of apoptosis, DNA damage, and repair. During an ATP assay, PHA provided high cytotoxicity to two oral cancer cell lines (CAL 27 and Ca9-22) but no cytotoxicity to two non-malignant oral cells (HGF-1 and SG). This selective antiproliferation of PHA was associated with the selective generation of reactive oxygen species (ROS) in oral cancer cells rather than in non-malignant oral cells, as detected by flow cytometry. Moreover, PHA induced other oxidative stresses in oral cancer cells, such as mitochondrial superoxide generation and mitochondrial membrane potential depletion. PHA also demonstrated selective apoptosis in oral cancer cells rather than non-malignant cells in annexin V/7-aminoactinmycin D and caspase 3/7 activity assays. In flow cytometry and immunofluorescence assays, PHA induced γH2AX expressions and increased the γH2AX foci number of DNA damages in oral cancer cells. In contrast, the mRNA expressions for DNA repair signaling, including homologous recombination (HR) and non-homologous end joining (NHEJ)-associated genes, were inhibited by PHA in oral cancer cells. Moreover, the PHA-induced changes were alleviated by the oxidative stress inhibitor N-acetylcysteine. Therefore, PHA generates selective antiproliferation, oxidative stress, and apoptosis associated with DNA damage induction and DNA repair suppression in oral cancer cells.     

磨机负荷检测系统无线数据采集装置的研制

针对球磨机振动信号采集及数据传输,研制了一套低功耗无线采集传输系统。通过对电路系统的降噪设计,提高了处理精度,使其完全满足数据采集的需要,解决了在线检测球磨机桶壁振动装置的连续使用问题。     

塔河油田底水砂岩油藏水平井剩余油主控因素分析

针对塔河油田一区、九区等强底水砂岩油藏高含水期油水关系复杂、影响因素多、剩余油认识不清造成措施难度大的特点,分析了不同主控因素下水淹模式及剩余油的形成和分布特征。从水平井轨迹与夹层不同的配置关系入手,将轨迹与夹层的配置关系分为全遮挡型、穿行夹层型、穿过夹层型和半遮挡型4种配置关系。根据渗透率组合形态将水平段渗透率组合分为4种形态:两段式、锯齿式、台阶式和均匀式,并结合油井生产动态研究了不同配置关系及渗透率形态所对应的水平段水淹模式,描述其对剩余油不同控制作用,并结合矿场试验给出各种剩余油赋存方式适合的挖潜手段,为底水油藏剩余油精细挖潜提供依据。     

从超振荡透镜到超临界透镜：超越衍射极限的光场调制

以超振荡透镜和超临界透镜为典型代表的平面超透镜是一种利用光场调控方式实现远场超衍射极限聚焦和成像的光学元件。通过精密调控各衍射结构单元之间的干涉效应,可以在焦平面上局部区域内获得高于系统最高空间频率的电场振荡,从而实现对衍射焦斑横向和轴向尺寸的可控调节。与传统的光学透镜相比,平面超透镜具有聚焦能力强,结构紧凑,设计自由度大,利于集成等优点。因其远场超衍射极限的光场调控能力,受到衍射光学和纳米光子学领域人员的广泛关注和研究。本文介绍了平面超衍射极限透镜光场调控的原理和设计方法。对超振荡透镜和超临界透镜的研究现状及其在远场光学超分辨成像领域的应用进行了分析和讨论,最后对该领域面临的问题及其拓展方向作了展望。

     

A portable optical sensing system for rapid detection of fluorescence spectra

Development of a prototype of a portable optical sensing system is presented for fast detecting of samples' fluorescence spectra. A compact configuration is achieved by integrating a small spectrometer, a microcontroller, a Universal Serial Bus (USB) Host Shield, a network module, and a web server. The fluorescence spectra of a tested sample can be obtained. Then the test data are sent through network communication to our Cloud Server which can store the data for further analyses. With this configuration, test results can be revealed in a short time and downloaded by users to their laptops, tablets or cellphones anytime and anywhere.     

BIM技术在深基坑支护预应力锚索施工中的应用

利用BIM技术,建立了湖南广电节目生产基地项目深基坑支护桩锚三维信息模型,实现了预应力锚索的预建造,辅助工程师提前发现了图纸中存在的问题,并采取相应措施进行了处理。通过BIM技术的应用,重点发现并解决了原设计图纸中预应力锚索的碰撞问题,将碰撞报告提交设计院进行了图纸优化设计,并制作了三维可视化交底动画,为项目深基坑支护工程按期、保质完成提供了强有力的技术支持,取得了良好的经济效益与社会效益。