An augmented model of brief psychodynamic interpersonal therapy for patients with nonepileptic seizures.

Nonepileptic seizures (NES) are one of the most common functional (medically unexplained) symptoms seen by neurologists. Although most experts consider psychotherapy the treatment of choice, few therapeutic approaches have been described in detail. Given that NES occur in the context of many different psychopathologies, it remains uncertain whether there is 1 intervention that can benefit all comers or whether it is necessary to offer individualized psychotherapy. This article describes an approach grounded in psychodynamic interpersonal therapy but augmented with elements of cognitive–behavioral therapy, somatic trauma therapy, and the involvement of caregivers and family members. The approach was developed in the setting of a specialist psychotherapy service for patients with functional neurological disorders presenting to British hospital-based neurologists. The authors have previously shown that it is associated with significant improvements in psychological functioning, health-related functioning, and a symptom count. Three case reports illustrate how the treatment can be adapted to meet different patients' needs. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Electrochemical reactivity of trihexyl(tetradecyl)phosphonium bis(2,4,4-trimethylpentyl)phosphinate ionic liquid on glassy carbon and AZ31 magnesium alloy

The electrochemical behaviour of the ionic liquid trihexyl(tetradecyl)phosphonium bis(2,4,4-trimethylpentyl)phosphinate ([P_6,6,6,14][(ⁱC₈)₂PO₂]) over different potential ranges, on both an inert substrate (glassy carbon), and AZ31 magnesium alloy has been investigated. On the glassy carbon electrode the ionic liquid exhibits an electrochemical window of 4 V in an argon environment. Anodic cycling of AZ31 in the ionic liquid resulted in a reduction in current density of over two orders of magnitude after one cycle and also stifled ionic liquid oxidation at approximately 2.0 V, indicating a passive response in the ionic liquid as a result of the deposition of a surface film. In contrast, cathodic cycling of AZ31 led to an increasing current density response with progressive cycles. The understanding of this electrochemical behaviour will allow for future optimisation of electrochemical parameters to obtain a robust and corrosion protective film on AZ31 alloy.     

The amino acid composition of the proteinaceous component of gum tragacanth (Asiatic Astragalus spp.)

Six Iranian and seven Turkish samples of commercial gum tragacanth, and a sample of Turkish 'gum traganton', have been studied. Their nitrogen content varied from 0.17 to 0.58%. Their amino acid compositions are characterized by the presence of very large but variable proportions of hydroxyproline and substantial proportions of serine, proline and valine. The data presented may be useful for extending the current specifications for identity and purity, at present based solely on polysaccharide parameters, for gum tragacanth (E413).     

Neuroprotective effects of lamotrigine in global ischemia in gerbils. A histological, in vivo microdialysis and behavioral study

A sudden surge in the release of glutamate is currently believed to be an important initiating step in neuronal damage due to an ischemic insult. In this experiment, we tested the efficacy of neuroprotection with lamotrigine, a novel antiepileptic drug that blocks voltage gated sodium channels and inhibits the ischemia-induced release of glutamate in the gerbil forebrain model of cerebral ischemia. The medication was administered 30 min before and 30 min after the insult in two groups of animals. Histological assessment of neuronal damage was evaluated at 7 and 28 days after the ischemic insult. Animals evaluated at 28 days also underwent behavioral testing. Microdialysis was used in the same model to study the response of ischemia-induced glutamate in saline treated controls versus animals treated with lamotrigine 20 min before the insult. There was highly significant neuronal protection in animals who were treated with lamotrigine either before or after the insult. Protection was seen both at 7 and 28 days after the insult. Behavioral testing also showed significantly better recovery in both sets of animals in comparison to the saline-treated group. Microdialysis confirmed a significant attenuation of the ischemia-induced glutamate surge when compared to the saline-treated animals. Our morphological, behavioral and microdialysis experiments show that lamotrigine offers significant neuroprotection from the effects of transient forebrain ischemia in gerbils. Neuroprotection with post-ischemic therapy probably depends on preserving the capacity of the sodium/calcium exchanger to reduce intracellular calcium concentrations or persistent 'toxicity' of glutamate in the reperfusion period on the already 'primed' injured neurons. These concepts need further study.     

Quantification of the bone-related mRNAs at the bone/prosthetic interface

Aseptic loosening is one of the major reasons for failure of joint prostheses. The periprosthetic tissue has previously been described microscopically; however, little work has been devoted towards quantitating genes expressed by cells at the materials/tissue interface. This study aims to characterize the phenotypic expression of osteoblasts and test the feasibility of quantifying the level of gene expression in periprosthetic tissue sections by combining in situ hybridization and image analysis techniques. There are many factors to consider when quantifying mRNA, in that comparing labeling between different cDNA probes, these should have comparable length and base comparison. The probes should be labeled with the same specific activity, that is the amount of probe to label added is the same, both between different probes and between batches of the same probe. Chromagen color reactions are variable in that the color development is not always linear and more likely follows a sigmoidal curve. Samples should only be compared when it is known that the reaction has been in the linear range. The image analysis of such staining introduces further factors which should be considered and controlled. Color analysis is a very complex problem with respect to reproducibly analyzing histological sections. The brightness component of the image should be independent of the colors within the image, in conventional RGB (red, green and blue) signalling mode this is not possible, while when using HSI (hue saturation and intensity) mode this becomes possible, and factors like staining intensity and brightness of the image become much more accountable and controllable. With these factors identified, we consider that the quantitative image analysis approach does allow comparison of patterns of bone-related mRNAs and demonstrates differences in expression in these osteogenic factors depending on distance from the prosthesis, tissue type, patient and device. © 1998 Kluwer Academic Publishers     

Ionic liquids in electrochemical devices and processes: managing interfacial electrochemistry

Many ionic liquids offer a range of properties that make them attractive to the field of electrochemistry; indeed it was electrochemical research and applications that ushered in the modern era of interest in ionic liquids. In parallel with this, a variety of electrochemical devices including solar cells, high energy density batteries, fuel cells, and supercapacitors have become of intense interest as part of various proposed solutions to improve sustainability of energy supply in our societies. Much of our work over the last ten years has been motivated by such applications. Here we summarize the role of ionic liquids in these devices and the insights that the research provides for the broader field of interest of these fascinating liquids.     

Synthesis and physical property characterisation of phosphonium ionic liquids based on P(O)2(OR)2 and P(O)2(R)2 anions with potential application for corrosion mitigation of magnesium alloys

Phosphonium cation based ionic liquids (ILs) have become of interest due to their unique chemical and electrochemical stability as well as their promising tribological properties. At the same time, interest has also grown in the use of phosphate and phosphinate based ionic liquids for corrosion protection of reactive metals. In this work we describe the synthesis and characterization of six novel ionic liquids based on the tetraalkylphosponium cation coupled with organophosphate and organophosphinate anions and their sulfur analogues. The conductivity and viscosity of these ILs has been measured and discussed in terms of the nature of the interactions, effect of anion basicity and the extent of ionic character. The reaction of the IL with a ZE41 magnesium aerospace alloy surface is also demonstrated.     

Solution structure of human neuropeptide Y

The three-dimensional structure of synthetic human neuropeptide Y in aqueous solution at pH 3.2 and 37 degrees C was determined from two-dimensional 1H NMR data recorded at 600 MHz. A restraint set consisting of 440 interproton distance restraints inferred from NOEs and 11 backbone and 4 side-chain dihedral angle restraints derived from spin-spin coupling constants was used as input for distance geometry calculations on DIANA and simulated annealing and restrained energy minimization in X-PLOR. The final set of 26 structures is well defined in the region of residues 11-36, with a mean pairwise rmsd of 0.51 A for the backbone heavy atoms (N, C alpha and C) and 1.34 A for all heavy atoms. Residues 13-36 form an amphipathic alpha-helix. The N-terminal 10 residues are poorly defined relative to the helical region, although some elements of local structure are apparent. At least one of the three prolines in the N-terminal region co-exists in both cis and trans conformations. An additional set of 24 distances was interpreted as intermolecular distances within a dimer. A combination of distance geometry and restrained simulated annealing yielded a model of the dimer having antiparallel packing of two helical units, whose hydrophobic faces form a well-defined core. Sedimentation equilibrium experiments confirm the observation that neuropeptide Y associates to form dimers and higher aggregates under the conditions of the NMR experiments. Our results therefore support the structural features reported for porcine neuropeptide Y [Cowley, D.J. et al. (1992) Eur. J. Biochem., 205, 1099-1106] rather than the 'aPP' fold described previously for human neuropeptide Y [Darbon, H. et al. (1992) Eur. J. Biochem., 209, 765-771].     

Polyterthiophene/CNT composite as a cathode material for lithium batteries employing an ionic liquid electrolyte

A polyterthiophene (PTTh)/multi-walled carbon nanotube (CNT) composite was synthesised by in situ chemical polymerisation and used as an active cathode material in lithium cells assembled with an ionic liquid (IL) or conventional liquid electrolyte, LiBF₄/EC-DMC-DEC. The IL electrolyte consisted of 1-ethyl-3-methylimidazolium tetrafluoroborate (EMIBF₄) containing LiBF₄ and a small amount of vinylene carbonate (VC). The lithium cells were characterised by cyclic voltammetry (CV) and galvanostatic charge/discharge cycling. The specific capacity of the cells with IL and conventional liquid electrolytes after the 1st cycle was 50 and 47 mAh g⁻¹ (based on PTTh weight), respectively at the C/5 rate. The capacity retention after the 100th cycle was 78% and 53%, respectively. The lithium cell assembled with a PTTh/CNT composite cathode and a non-flammable IL electrolyte exhibited a mean discharge voltage of 3.8 V vs Li⁺/Li and is a promising candidate for high-voltage power sources with enhanced safety.     

Lithium Sulfonate Functionalization of Carbon Cathodes as a Substitute for Lithium Nitrate in the Electrolyte of Lithium–Sulfur Batteries

A method for grafting lithium sulfonate (LiSO₃) groups to carbon surfaces is developed and the resulting carbons are evaluated for their potential to reduce the lithium polysulfide (LiPS) shuttle in lithium–sulfur (Li–S) batteries, replacing the common electrolyte additive lithium nitrate (LiNO₃). The LiSO₃ groups are attached to the ordered mesoporous carbon (CMK3) surface via a three‐step procedure to synthesize LiSO₃‐CMK3 by bromomethylation, sodium sulfite (Na₂SO₃) substitution, and cation exchange. As a comparison, ethylenediamine (EN)‐substituted CMK3, EN‐CMK3, is also synthesized and tested. When used as a cathode in Li–S batteries, the unfunctionalized CMK3 suffers from strong LiPS shuttling as evidenced by its low initial Coulombic efficiencies (ICEs, <10%) compared to its functionalized derivatives EN‐CMK3 and LiSO₃‐CMK3 (ICEs >75%). Postcycling analysis reveals the benefits of cathode surface functionalization on the lithium anode via an attenuated LiPS shuttle. When monitored at open circuit, the functionalized cathodes maintain their cell voltages much better than the CMK3 control and concurrent electrochemical impedance spectroscopy reveals their higher total cell resistance, which provides evidence for a reduced LiPS shuttle in the vicinity of both electrodes. Overall, such surface groups show promise as cathode‐immobilized “lithium nitrate mimics.”