Sensing the insulin signaling pathway with an antibody array

The development of insulin resistance and type 2 diabetes is determined by various factors, including defects within the insulin signaling pathway. Mediators of insulin resistance operate through activation of various protein kinase C isoforms, IκB kinase β (IKKβ), and/or c‐Jun N‐terminal kinase, and subsequent inhibition of the proximal insulin signaling pathway via the insulin receptor substrate 1 and Akt. These mechanisms are still largely unresolved because of the complexity of the molecular events. In this study, an expression and activation state profiling of multiple known key signaling biomolecules involved in insulin metabolic and mitogenic signaling pathways was evaluated using a phosphospecific antibody array platform. The results of the arrayed antibodies were verified by the multiplexed bead array assay and conventional Western blot analysis, and confirmed the well‐known inhibitory effects of phorbol esters on insulin signaling pathway activation. Of interest, the increase in protein kinase C signaling responses with phorbol esters was associated with activation of the lipid phosphatase PTEN and a 27 kDa HSP. Thus, this insulin signaling antibody array provides a powerful and effective way to investigate the mechanism of insulin resistance and likely assist the development of innovative therapeutic drugs for type 2 diabetes.     

基于领域工程的构件化可扩展管理信息系统

为使管理信息系统（MJS）适应不断变化的用户需求,在基于领域工程的软件开发方法和构件技术基础上,对MIS的领域分析、领域设计和领域构件的实现进行详细研究;并在基于Struts、Spring和Hibernate三种组合框架支持下,利用领域模型的分层结构思想,提出了一种具有数据抽象与细粒化、松耦合层次风格相结合的软件体系结构,在此结构上结合构件技术和代码自动生成技术解决了MIS因环境或服务变化引起的系统需求变化,为MIS及相关系统因需求变化引起的领域对象及相关业务操作变化提供了一种合理的技术解决方案。     

UMXGIS的设计与实现①

WebGIS地理信息系统随着web应用的发展而发展,传统的基于web服务器端服务与基于桌面的客户端服务相独立的模式已经不能满足现实社会生产生活的需求。因此提出优化的基于Web Services的2N模式,该模式使服务器和客户端两者之间数据交互更便捷。通过遴选,整合,优化相关技术,设计并实现了基于2N模式的UMXGIS系统。     

A method for topological entity correspondence in a replicated collaborative CAD system

In order to utilize the potential advantages of replicated collaborative CAD system to support natural, free, fast and less-constrained multi-user human-to-human interaction, a local-locking mechanism, which can provide fast modeling response, is adopted as concurrency control mechanism for a replicated on-line collaborative CAD system. A human–human interactive modeling is achieved by immediate local execution of modeling operations and exchange of modeling operations across all collaborative sites real-time. In particular, an approach to achieve topological entity correspondence across collaborative sites during modeling procedure, which is critical to guarantee the correctness and consistency of collaborative modeling result, is proposed. A prototype system based on the ACIS geometric modeling kernel is implemented to verify availability of the proposed solution.     

RCCtrust: A Combined Trust Model for Electronic Community

Previous trust models are mainly focused on reputational mechanism based on explicit trust ratings. However, the large amount of user-generated content and community context published on Web is often ignored. Without enough information, there are several problems with previous trust models: first, they cannot determine in which field one user trusts in another, so many models assume that trust exists in all fields. Second some models are not able to delineate the variation of trust scales, therefore they regard each user trusts all his friends to the same extent. Third, since these models only focus on explicit trust ratings, so the trust matrix is very sparse. To solve these problems, we present RCCtrust – a trust model which combines Reputation-, Content- and Context-based mechanisms to provide more accurate, fine-grained and efficient trust management for the electronic community. We extract trust-related information from user-generated content and community context from Web to extend reputation-based trust models. We introduce role-based and behavior-based reasoning functionalities to infer users' interests and category-specific trust relationships. Following the study in sociology, RCCtrust exploits similarities between pairs of users to depict differentiated trust scales. The experimental results show that RCCtrust outperforms pure user similarity method and linear decay trust-aware technique in both accuracy and coverage for a Recommender System.     

高效液相色谱法同时测定保健食品中9种非法添加降脂类药物

目的建立采用高效液相色谱法同时测定调节血脂类保健食品中烟酸、普伐他汀钠、瑞舒伐他汀钙、苯扎贝特、阿托伐他汀钙、氟伐他汀钠、吉非罗齐、洛伐他汀及辛伐他汀9种非法添加药物的方法。方法以甲醇为样品提取溶剂,采用Inertsil ODS-3色谱柱,以0.02 mol/L乙酸铵-甲醇为流动相梯度洗脱;流速为1.0m L/min;检测波长为230 nm,柱温为30℃,利用保留时间定性、外标法定量,分析9种非法添加药物。结果本方法可以在20 min内定性并定量分析烟酸、普伐他汀钠、瑞舒伐他汀钙、苯扎贝特、阿托伐他汀钙、氟伐他汀钠、吉非罗齐、洛伐他汀及辛伐他汀9种化学药物。9种非法添加药物在1.0~100μg/m L之间线性关系良好,相关系数均大于0.999;除阿托伐他汀钙在袋泡茶中回收率较低外,平均加标回收率范围为77.9%~102.9%,相对标准偏差范围为0.42%~5.8%。结论该方法线性关系良好,线性范围广,准确度及精密度高,可同时鉴定是否非法添加上述9种化学药物。     

关于采场测量方法及其准确率之研究

采场测量方法多种多样, 一般以简单快捷、工作量少与准确率高为目的, 对三鑫公司常用的采场测量方法加以总结并予以评述, 在此基础上提出一种新的采场测量方法-射线法。     

Effect of easily degradable substrate on anaerobic degradation of pentachlorophenol in an upflow anaerobic sludge blanket (UASB) reactor

The effect of microbial easily degradable substrate (MEDS) on the anaerobic degradation of pentachlorophenol (PCP) in two upflow anaerobic sludge blanket (UASB) reactors was investigated. The results indicated that glucose-utilizing activity decreased with the increase of PCP concentration in the mixed culture, and MEDS promoted PCP-dechlorination and degrading activities. The concentration of MEDS increased from 917 to 4580 mg L(-1) with the increase of PCP concentration from 100 to 181 mg L(-1)in influent, which was necessary for maintenance of steady operation of the experimental reactors, the removal rate of PCP and COD ranged up to 99.5 and 90.0% and the concentration of PCP in the effluent was less than 0.5 mg L(-1). The concentration of PCP in effluent was linearly or logarithmically related to sucrose concentration in the influent while PCP was less than the maximum permissible PCP concentration. The activity of anaerobic sludge in the reactor decreased as the concentration of PCP increased, but it could be recovered step by step as time progressed. In the lowest layer of the reactor, the activity of sludge was the highest. So it is feasible to accelerate the degradation of the organic toxic compounds like PCP, by adding suitable quantities of microbial easily degradable substrate to the system.     

装饰工程中厨卫渗漏防治措施探讨

对建筑中的厨房和卫生间的渗漏现象及其渗漏原因进行了简要的概述及分析,指出了建筑中进行厨卫间装修防水的必要性.同时针对厨卫中所出现的渗漏问题,从装饰工程的设计、材料选择和施工等方面提出了解决渗漏问题的措施与方法,指出在防水设计时应注意的问题,以提高住宅建筑的防水性能和耐久性.     

Revealing Atomic Configuration and Synergistic Interaction of Single-Atom-Based Zn-Co-Fe Trimetallic Sites for Enhancing Oxygen Reduction and Evolution Reactions

Anchoring single metal atom to carbon supports represents an exceptionally effective strategy to maximize the efficiency of catalysts. Recently, dual-atom catalysts (DACs) emerge as an intriguing candidate for atomic catalysts, which perform better than single-atom catalysts (SACs). However, the clarification of the polynary single-atom structures and their beneficial effects remains a daunting challenge. Here, atomically dispersed triple Zn-Co-Fe sites anchored to nitrogen-doped carbon (ZnCoFe-N-C) prepared by one-step pyrolysis of a designed metal-organic framework precursor are reported. The atomically isolated trimetallic configuration in ZnCoFe-N-C is identified by annular dark-field scanning transmission electron microscopy and spectroscopic techniques. Benefiting from the synergistic effect of trimetallic single atoms, nitrogen, and carbon, ZnCoFe-N-C exhibits excellent catalytic performance in bifunctional oxygen reduction/evolution reactions in an alkaline medium, outperforming other SACs and DACs. The ZnCoFe-N-C-based Zn-air battery exhibits a high specific capacity (liquid state: 931.8 Wh kg_Zn⁻¹), power density (liquid state: 137.8 mW cm⁻²; all-solid-state: 107.9 mW cm⁻²), and good cycling stability. Furthermore, density-functional theory calculations rationalize the excellent performance by demonstrating that the ZnCoFe-N-C catalyst has upshifted d-band center that enhances the adsorption of the reaction intermediates.