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971.
Electroactive polymers have shown promising applications as transducers that can mimic biological muscle. The modulus or the compliance of many of these devices can change significantly as they are actuated making these materials attractive for applications that require tunable stiffness. We have developed a dynamic mechanical analyzer that is capable of making in situ measurements of the dynamic compliance transfer function of conducting polymers as a function of an electrochemical stimulus. We do this by simultaneously applying a stochastic stress waveform over a potential waveform and calculating the compliance as it changes over the course of electrochemical excitation. Using these signals we can calculate the compliance transfer function between 0.1 and 100 Hz and the impulse response function with up to 3% variation in its parameters. These functions are then computed as charge is injected into the polymer and it is shown that the low frequency gain of the transfer function can change by 30%-40% in the electrochemical system tested. 相似文献
972.
Gerneke DA Sands GB Ganesalingam R Joshi P Caldwell BJ Smaill BH Legrice IJ 《Microscopy research and technique》2007,70(10):886-894
Three-dimensional reconstruction of large tissue volumes using histological thin sections poses difficulties because of registration of sections, section distortion, and the possibility of incomplete data set collection due to section loss. We have constructed an integrated surface imaging system that successfully addresses these problems. Embedded tissue is mounted on a high precision XYZ stage and the upper surface is iteratively: (i) stained to provide an effective optical section, (ii) imaged using a digital camera, and (iii) removed with an ultramiller. This approach provides for the reconstruction of high-quality 3D images by inherently preserving image registration, eliminates section distortion, thus removing the need for complex realignment and correction, and also ensures full capture of all image planes. The system has the capacity to acquire images of tissue structure with voxel sizes from 0.5 to 50 mum over dimensions ranging from micrometers to tens of millimeters. The ultramiller enables large samples to be imaged by reliably removing tissue over their full extent. The ability to visualize key features of 3D tissue structure across such a range of scale and resolution will facilitate the development of a greater understanding of the relationship between structure and function. This understanding is essential for better analyses of the structural changes associated with different disease states, and the development of structure-based computer models of biological function. 相似文献
973.
Applications of accelerator mass spectrometry for pharmacological and toxicological research 总被引:2,自引:0,他引:2
The technique of accelerator mass spectrometry (AMS), known for radiocarbon dating of archeological specimens, has revolutionized high-sensitivity isotope detection in pharmacology and toxicology by allowing the direct determination of the amount of isotope in a sample rather than measuring its decay. It can quantify many isotopes, including 26Al, 14C, 41Ca, and 3H with detection down to attomole (10(-18)) amounts. Pharmacokinetic data in humans have been achieved with ultra-low levels of radiolabel. One of the most exciting biomedical applications of AMS with 14C-labeled potential carcinogens is the detection of modified proteins or DNA in tissues. The relationship between low-level exposure and covalent binding of genotoxic chemicals has been compared in rodents and humans. Such compounds include heterocyclic amines, benzene, and tamoxifen. Other applications range from measuring the absorption of 26Al to monitoring 41Ca turnover in bone. In epoxy-embedded tissue sections, high-resolution imaging of 14C label in cells is possible. The uses of AMS are becoming more widespread with the availability of instrumentation dedicated to the analysis of biomedical samples. 相似文献
974.
De Beule P Owen DM Manning HB Talbot CB Requejo-Isidro J Dunsby C McGinty J Benninger RK Elson DS Munro I John Lever M Anand P Neil MA French PM 《Microscopy research and technique》2007,70(5):481-484
We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high-speed FLIM technology in a line-scanning microscope. We demonstrate the acquisition of whole-field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue. 相似文献
975.
The moisture sorption response of commercial OSB panels at room temperature were examined as a function of humidity. The OSB panels were pine OSB—southern pine (Pinus spp.) mixed with 10–20% of hardwood, usually maple (Acer spp.); aspen OSB—nearly 100% aspen (Populas tremuloides Michx.); and, poplar OSB—40% yellow-poplar (Liriodendron tulipifera L.), 20% maple (Acer spp.), 10% pine and 30% unknown mixture. Two panels from each species group of OSB were chosen based on resin of phenol formaldehyde (PF) face and methyl di-isocyanate core or PF face and PF core. The multi-step sorption isotherm was modeled with the GAB isotherm model and the A/D ratio was determined from the data. Using the model, it was found that the heats of sorption were 6–8 times increased in OSB compared to solid wood. Also, based on the model analysis, the unimolecular layer was smaller compared to solid wood. The average A/D ratio, which is related to the hysteresis, was smaller compared to solid wood. 相似文献
976.
Ian D. Watson Peter S. Brandon rew Basden 《Computer-Aided Civil and Infrastructure Engineering》1991,6(4):303-310
Abstract: This paper describes the development of a knowledge Based System for predicting strategic maintenance in residential property. An initial feasiility study identified a need for the long term strategic planning of building maintence in Housing Association. However, conventional Maintenance Management programs require too much data input, are too inflexible, do not explain their results, and not permit their users to explore potential alternatives. The application of knowledge Based Systems's technology to this area directly addresses these problems. This paper examines the need for such systems, and describes the implementation of one such system using a Client Centered Mehodology. The paper concludes by discussing some of the problems that were encourtered during the systems's development, how they were overcome, and what lessons may be learned for future systems developmets. 相似文献
977.
The influence of soil remediation on lead in house dust 总被引:5,自引:0,他引:5
von Lindern IH Spalinger SM Bero BN Petrosyan V von Braun MC 《The Science of the total environment》2003,303(1-2):59-78
Lead in house dust has long been recognized as a principal source of excess lead absorption among children at the Bunker Hill Superfund Site (BHSS) in northern Idaho. House dust lead concentration from homeowner's vacuum cleaner bags has been monitored since the epidemic of childhood lead poisoning in 1974. Geometric mean house dust lead concentrations decreased from >10000 mg/kg in 1974 to approximately 4000 mg/kg in 1975, in response to air pollution control initiatives at the defective primary lead smelter. After smelter closure, 1983 mean dust lead concentrations were near 3000 mg/kg and were most dependent on soil sources. Following emergency soil removals from public areas and roadsides and fugitive dust control efforts in the mid-1980s, house dust lead decreased by approximately 40-60% to 1200-1500 mg/kg. In 1992, a cleanup goal of 500 mg/kg dust lead community average, with no individual home exceeding 1000 mg/kg, was adopted. This goal was to be achieved by a combination of contaminated soil removals and fugitive dust control efforts throughout the 21 square mile BHSS. Continual reductions in house dust lead concentrations have been noted throughout the residential area soil cleanup. Geometric mean house dust lead concentrations averaged approximately 500-600 mg/kg from 1996 to 1999 and dropped below 500 mg/kg in 2000. Analysis of these data indicates that approximately 20% of the variance in dust lead concentrations is attributed to yard, neighborhood, and community soil lead concentrations. Since 1996, dust lead concentrations and dust and lead loading rates have also been measured by dust mats placed at entryways into the homes. Neighborhood soil lead concentrations, household hygiene, the number of adults living in the home, and the number of hours a child spends outdoors in summer explain approximately 26% of the variance in mat dust lead loading rates. It is estimated that post-remedial house dust lead concentrations will stabilize at 400-500 mg/kg, as compared to approximately 200 mg/kg in socio-economically similar background communities; the difference possibly attributed to residual soil concentrations (3-6 times background), recontamination of rights-of-way, tracking of non-residential mining district soils and dusts, fugitive dusts associated with the remediation, and residual structural or carpet dusts. 相似文献
978.
Effect of medium-pressure UV irradiation on bromate concentrations in drinking water, a pilot-scale study 总被引:1,自引:0,他引:1
This study investigated the potential for bromate removal from drinking water on irradiation with medium-pressure UV lamps-a technique gaining considerable interest for drinking water disinfection. Waters from two different sources were spiked with 20microg/L of bromate and irradiated with UV fluences up to 718mJ/cm(2) utilizing a pilot-scale reactor (Calgon Carbon Corp.) at a flow of 76L/min (20 gallon/min). Essentially no removal was observed in one of the source waters. Limited bromate removal, up to 19%, was observed in the second source water at high UV fluences (696mJ/cm(2)) and a fluence-response relationship was clearly evident. All removals would be negligible at UV fluences anticipated for drinking water disinfection (< or =40mJ/cm(2)). Different water characteristics, in particular competitive absorption by nitrate and possibly DOC, were most likely responsible for the differences in bromate removal in the waters tested. The source water that did not show any removal had a higher nitrate concentration (4 vs. 0.1mg N/L) and also a higher DOC concentration (4.1 vs. 3.1mg C/L) than the other source water which showed 19% bromate removal. 相似文献
979.
Scrip, or artificial currency, is a useful tool for designing systems that are robust to selfish behavior by users. However, it also introduces problems for a system designer, such as how the amount of money in the system should be set. In this paper, the effect of varying the total amount of money in a scrip system on efficiency (i.e., social welfare—the total utility of all the agents in the system) is analyzed, and it is shown that by maintaining the appropriate ratio between the total amount of money and the number of agents, efficiency is maximized. This ratio can be found by increasing the money supply to just below the point that the system would experience a “monetary crash,” where money is sufficiently devalued that no agent is willing to perform a service. The implications of the presence of altruists, hoarders, sybils, and collusion on the performance of the system are examined. Approaches are discussed to identify the strategies and types of agents. 相似文献
980.
Amrita Bose Muy-Teck Teh Ian C. Mackenzie Ahmad Waseem 《International journal of molecular sciences》2013,14(10):19385-19398
Keratin 15 (K15) is type I keratin protein co-expressed with the K5/K14 pair present in the basal keratinocytes of all stratified epithelia. Although it is a minor component of the cytoskeleton with a variable expression pattern, nonetheless its expression has been reported as a stem cell marker in the bulge of hair follicles. Conversely, suprabasal expression of K15 has also been reported in both normal and diseased tissues, which is inconsistent with its role as a stem cell marker. Our recently published work has given evidence of the molecular pathways that seem to control the expression of K15 in undifferentiated and differentiated cells. In this article, we have critically reviewed the published work to establish the reliability of K15 as an epidermal stem cell marker. 相似文献