Virtual Private Ad Hoc Networking

The fact that a lot of applications require secure communication to take place only between a dynamic subset of distributed devices sharing a common context, is, from a network point of view, very challenging and demanding. Existing technologies such as VPN, P2P overlays or VLANs can only partially respond to these requirements. This observation is the key factor that has driven the proposal of the virtual private ad hoc network concept. Virtual private ad hoc networks (VPAN) are secure and self-organizing overlay networks on top of existing IP infrastructure that use ad hoc networking techniques to enable network connectivity. The underlying IP infrastructure can be the Internet, cellular networks, ad hoc networks, mesh networks … or combinations thereof. A virtual private ad hoc overlay network creates a transparent, shielded and trusted environment for the applications and services running on the participants' devices. The overlay uses internal addressing and ad hoc routing, thereby forming a virtual network on top of the physical infrastructure. In addition, the overlay must be self-organizing and self-maintaining upon member mobility or membership changes. This paper gives an overview of the potential applications, a high-level network architecture and the network challenges emerging from the novel concept of virtual private ad hoc networking. Jeroen Hoebeke was born in Ghent, Belgium in 1979. In 2002 he received the Masters degree in engineering (Computer Science) from the University of Ghent. In August 2002, he joined the Broadband Communications Networks Group. His PhD research includes the development of adaptive routing protocol techniques for mobile ad hoc networks. His main research interests are in ad hoc wireless communications and, more generally, in broadband wireless communications. Within the European MAGNET project, he is actively involved in the development of a network architecture and demonstrator for Personal Networks, with a prime focus on routing and connectivity. Gerry Holderbeke was born in Zottegem, Belgium in 1982. He graduated in Informatics at the University of Ghent in 2004. In August 2004 he joined the Broadband Communications Networks Group where he is currently working as a project developer. His research currently includes the development of an emulator for mobile ad hoc networks. His main research interests are in ad hoc networks and broadband wireless communications and involve routing, addressing and more generally, communication within mobile ad hoc networks and infrastructured networks. Within the European MAGNET project, he is actively involved in the development of a network architecture for Personal Networks, with a prime focus on the implementation of the routing architecture. Ingrid Moerman was born in Gent, Belgium in 1965. She received the degree in Electro-technical Engineering and the Ph.D degree from the Ghent University, Gent, Belgium in 1987 and 1992, respectively. Since 1987, she has been with the Interuniversity Micro-Electronics Centre (IMEC) at the Department of Information Technology (INTEC) of the Ghent University, where she conducted research in the field of optoelectronics. In 1997, she became a permanent member of the Research Staff at IMEC. Since 2000 she is part-time professor at the Ghent University. Since 2001 she has switched her research domain to broadband communication networks. She is currently involved in the research and education on broadband mobile & wireless communication networks and on multimedia over IP. The main research topics related to mobile & wireless communication networks are: wireless access to vehicles (high bandwidth & driving speed), adaptive QoS routing in wireless ad hoc networks, body area networks, protocol boosting on wireless links, design of fixed access/metro part, traffic engineering and QoS support in the wireless access network. Ingrid Moerman is author or co-author of more than 300 publications in the field of optoelectronics and communication networks. Bart Dhoedt received a degree in Engineering from the Ghent University in 1990. In September 1990, he joined the Department of Information Technology of the Faculty of Applied Sciences, University of Ghent. His research, addressing the use of micro-optics to realize parallel free space optical interconnects, resulted in a PhD degree in 1995. After a 2 year post-doc in opto-electronics, he became professor at the Faculty of Applied Sciences, Department of Information Technology. Since then, he is responsible for several courses on algorithms, programming and software development. His research interests are software engineering and mobile & wireless communications. Bart Dhoedt is author or co-author of approximately 70 papers published in international journals or in the proceedings of international conferences. His current research addresses software technologies for communication networks, peer-to-peer networks, mobile networks and active networks. Piet Demeester received the Masters degree in Electro-technical engineering and the Ph.D degree from the Ghent University, Gent, Belgium in 1984 and 1988, respectively. In 1992 he started a new research activity on broadband communication networks resulting in the IBCN-group (INTEC Broadband communications network research group). Since 1993 he became professor at the Ghent University where he is responsible for the research and education on communication networks. The research activities cover various communication networks (IP, ATM, SDH, WDM, access, active, mobile), including network planning, network and service management, telecom software, internetworking, network protocols for QoS support, etc. Piet Demeester is author of more than 300 publications in the area of network design, optimization and management. He is member of the editorial board of several international journals and has been member of several technical program committees (ECOC, OFC, DRCN, ICCCN, IZS, &).     

Drug-induced reinstatement of heroin- and cocaine-seeking behaviour following long-term extinction is associated with expression of behavioural sensitization

The present study was designed to evaluate the relationship between reinstatement of drug-seeking behaviour following long-term extinction of intravenous (i.v.) drug self-administration (an animal model for craving) and long-term behavioural sensitization. Rats were allowed to self-administer heroin (50 microg/kg per inj., 14 daily sessions), cocaine (500 microg/kg per inj., 10 daily sessions) or saline. Following a 3-week extinction period, reinstatement tests were performed to evaluate priming effects of amphetamine, cocaine and heroin on nonreinforced drug-seeking behaviour. In addition, the occurrence of long-term behavioural sensitization in rats with a history of heroin or cocaine self-administration was determined. Heroin-seeking behaviour was reinstated by heroin (0.25 mg/kg), amphetamine (1.0 mg/kg) and cocaine (10 mg/kg). In addition, animals with a history of heroin self-administration displayed locomotor sensitization to both heroin and amphetamine. Cocaine-seeking behaviour was reinstated by cocaine and amphetamine, but not by heroin. Interestingly, locomotor sensitization to amphetamine, but not heroin, was observed in animals with a history of cocaine self-administration. In other words, the induction of drug-seeking behaviour following a prolonged drug-free period was found to be associated with the expression of long-term behavioural sensitization. These data provide experimental evidence for a role of behavioural sensitization in the incentive motivation underlying drug-seeking behaviour. If drug hyperresponsiveness would indeed be a crucial factor in drug-induced craving in human addicts, pharmacological readjustment of the neuroadaptations underlying drug sensitization may prevent relapse to drug use long after detoxification.     

Aggravated decrease in the activity of nucleus basalis neurons in Alzheimer's disease is apolipoprotein E-type dependent

As reported before, the metabolic activity of nucleus basalis neurons is reduced significantly in Alzheimer patients. Because the apolipoprotein E (ApoE) epsilon4 genotype is a major risk factor for Alzheimer's disease (AD), we determined whether the decrease in metabolic activity in nucleus basalis neurons in AD is ApoE-type dependent. The size of the Golgi apparatus (GA) was determined as a measure of neuronal metabolic activity in 30 controls and 41 AD patients with a known ApoE genotype by using an image analysis system in the nucleus basalis of Meynert. A polyclonal antibody directed against MG-160, a sialoglycoprotein of the GA, was used to visualize this organelle. There was a very strong reduction in the size of the GA in the nucleus basalis of AD patients. Furthermore, a strong and significant extra reduction in the size of the GA was found in the nucleus basalis neurons of AD patients with either one or two ApoE epsilon4 alleles compared with Alzheimer patients without ApoE epsilon4 alleles. Our data show that the decreased activity of nucleus basalis neurons in AD is ApoE epsilon4 dependent and suggest that ApoE epsilon4 participates in the pathogenesis of AD by decreasing neuronal metabolism.     

Modeling the Potential Impacts of Different Radon Policies for the U.S. Housing Stock

According to the Environmental Protection Agency (EPA) and other public health agencies in the United States, radon may be the leading cause (along with passive smoking) of lung cancer deaths among nonsmokers. Radon is estimated to be the second leading cause of lung cancer death in smokers behind smoking-related lung cancer. EPA estimates that 7,000 to 30,000 lung cancer deaths each year are due to radon exposure. (It is implied that radon-related lung cancer deaths can be prevented by reducing radon levels below EPA's guideline levels.) Current EPA radon policy is based on a strategy of education, the transfer of testing and remediation technologies to the public and private sectors, and recently proposed radon-resistant construction standards for new homes. This paper models the effectiveness of current, proposed, and alternative policies for reducing radon risks in U.S. residential construction. The results of our analysis suggest that EPA's projections of 2,200 “lives saved annually” as a result of its current action level of 4 pCi/l will not be achieved with its current policy in the near future. Overall, the response of radon-related mortality to most policy options is delayed and flat due in pan to the large number of houses with low radon levels and the long latency period between radon exposure and the development of cancer. The modeling results suggest that more aggressive smoking reduction programs may yield greater benefits in overall lung cancer mortality (but not reduced radon exposure) than most radon-related policies.     

Continuous Determination of the Oxidation State in a Soda-Lime-Silica Glassmelt During Refining

Influences on the oxidation state of glassmelts can be studied by using an electrochemical oxygen sensor which allows in-situ measurements at melting temperatures. The action of fining agents such as arsenic and sodium sulfate was investigated. The behavior of arsenic (i.e. release and uptake of oxygen) and the decomposition of sodium sulfate with and without carbon additions can be seen electrochemically.     

Selenium biotransformations in an insect ecosystem: effects of insects on phytoremediation

Phytoremediation of selenium-contaminated soils may be influenced by higher trophic levels including insects. We examined how selenium affects the behavior, survival, and development of the wasp parasitoid Cotesia marginiventris, parasitizing its natural host, the beet armyworm Spodoptera exigua, feeding on alfalfa, Medicago sativa, irrigated with water containing selenate. X-ray absorption spectroscopy was used to quantify the selenium chemical forms in each trophic level. Alfalfa partially transformed selenate to organoselenium. S. exigua contained only organoselenium, both directly absorbed from M. sativa and transformed from selenate. C. marginiventris cocoons collected shortly after larval emergence contained only organoselenium derived from the host. The surprising finding of trimethylselenonium-like species in adult parasitoids and the cocoons from which they emerged suggests that adults and pharates can detoxify excess selenium through methylation and volatilization. Adult parasitoids do not discriminate against selenium-containing alfalfa, even though alfalfa generates selenium volatiles. Parasitoids raised on selenium-fed larvae emerged later and pupae weighed less than their selenium-free counterparts. We conclude therefore that C. marginiventris can be used to control S. exigua damage to M. sativa being used to remove selenium from soils. Moreover, the presence of such insects may improve phytoremediation by increasing biotransformation of inorganic selenium and release of volatile selenium species.     

Characterization of the binding site for inhibitors of the HPV11 E1-E2 protein interaction on the E2 transactivation domain by photoaffinity labeling and mass spectrometry

An indandione-containing class of inhibitors abrogates DNA replication of human papillomavirus (HPV) types 6 and 11 by binding reversibly to the transactivation domain (TAD) of the viral E2 protein and inhibiting its interaction with the viral E1 helicase. To locate the binding site of this class of protein-protein interaction inhibitors, a benzophenone derivative was used to generate an irreversibly labeled E2-TAD polypeptide. The single site of covalent modification of the E2-TAD was identified by proteolytic digestions using trypsin, LysC, and V8 proteases and characterization of the resulting peptides by LC-MS procedures. Through this methodology, the benzophenone attachment point was located at the terminal methyl of residue Met101. Evidence further pinpointed the site of photoaffinity attachment to the terminal carbon atom, which is significant in providing a definitive example of the ability to locate photoinduced cross-linking to a polypeptide with atomic resolution using solely mass spectrometric detection. The location of the inhibitor binding site vis-à-vis the Glu39 and Glu100 residues sensitive to mutation for HPV 11 E2-TAD is discussed in relation to the crystal structure of the E2-TAD from the related HPV type 16.