Morphometric and some immunohistochemical characteristics of human choroids plexus stroma and psammoma bodies

Psammoma bodies (PBs) are one of many choroids plexus aging changes. The aim of our research was to perform the quantification of PBs' presence in human choroids plexus stroma, as well as to evaluate the characteristics of choroids plexus stroma in cases in which PBs were present. Afterwards, the observations of the histochemical analysis would be confirmed by immunohistochemical analysis. Choroid plexuses of 30 cadavers were used for the histochemical and, choroids plexuses of 15 cadavers in which PBs' presence was confirmed during the histochemical analysis, were used as material for the immunohistochemical analysis. Light microscopy, histochemical, immunohistochemical, and morphometric method were applied during the study. Classification of the cases was performed by cluster analysis. We observed increase of choroids plexus PBs' presence during the aging process. But this increase is not linear. Their presence is the largest in the second cluster that is younger than the third and older than the first. Nuclear morphometric parameters of the stroma in these cases showed that the cellular composition in this cluster is different than in other two and, that contain larger number of lymphoid cells. Immunohistochemical analysis showed PBs' positive reaction on vimentin, CD45R0, and LCA markers, while in their vicinity, as well as inside them, numerous T-cells were observed. So, the presence of CD45R0 and LCA-positive T cells, PBs' positive reaction on the same markers, indirectly connect these cells with PBs' formation process.     

Effective dimensionality of cuprate superconductors

A novel optical method for accurate monitoring of the carrier densityN in layered conductors is introduced. It is applied to several cuprate superconductors, and it is found thatT _c doesnot scale withN. However, if the closely spaced pairs or triplets of CuO₂ planes were coupled together to act as distinct metallic slabs, the relevant variable could ben, theslab carrier density. Indeed, we found thatT _c does scale nicely withn. This suggests that high-T _c superconductivity is effectively a two-dimensional phenomenon; it can occur in a cuprate slab which is only one unit cell thick. Finally, estimates are obtained for the length scales of localization of charge carriers:d 8 Å andd 2 Å in all the cuprates under study.     

The Kosko Subsethood Fuzzy Associative Memory (KS-FAM): Mathematical Background and Applications in Computer Vision

Many well-known fuzzy associative memory (FAM) models can be viewed as (fuzzy) morphological neural networks (MNNs) because they perform an operation of (fuzzy) mathematical morphology at every node, possibly followed by the application of an activation function. The vast majority of these FAMs represent distributive models given by single-layer matrix memories. Although the Kosko subsethood FAM (KS-FAM) can also be classified as a fuzzy morphological associative memory (FMAM), the KS-FAM constitutes a two-layer non-distributive model. In this paper, we prove several theorems concerning the conditions of perfect recall, the absolute storage capacity, and the output patterns produced by the KS-FAM. In addition, we propose a normalization strategy for the training and recall phases of the KS-FAM. We employ this strategy to compare the error correction capabilities of the KS-FAM and other fuzzy and gray-scale associative memories in terms of some experimental results concerning gray-scale image reconstruction. Finally, we apply the KS-FAM to the task of vision-based self-localization in robotics.     

Influence of oxygen content on some properties of Nb3Sn tape used for superconducting transmission lines

The influence of the presence of oxygen in the Nb-Zr substrate upon some physical and mechanical properties of the Nb-ZrO₂ substrate and/or Nb₃Sn tape superconductor prepared from such a substrate has been studied. The results have shown that the Nb₃Sn grains are much finer in the case of Nb-ZrO₂, than those obtained on Nb-Zr substrate. As a result, both the electrical and mechanical properties have been substantially improved. The critical current at a field of 5 T increased by between 100 and 200 %, the alternating current losses decreased by about one order of magnitude, the tensile strength increased by about a factor of three and the microhardness value also increased by about a factor of three for oxygen contents in the range 0.03 to 0.70 wt%. The specific resistance of this substrate increased at the same time from 17 to 22 cm and the relative elongation decreased from 5 to 3 %.     

A rough set approach to design concept analysis in a design chain

The inherent dynamic relationships among design tasks performed concurrently at different organizations characterize the complexities of a design chain where designers with diverse expertise need to collaborate across organizational boundaries. To ensure timely completion of inter-related design tasks, metrics to facilitate the early evaluation of design concepts are crucial. The ability to evaluate and select suitable design concepts at an early stage will ensure better solutions and greater savings in time and effort further downstream. This paper proposes a new approach based on the rough set theory to design concept analysis. The approach aims at early detection of design inadequacy. A so-called information system is constructed using the information gleaned from design concepts and design capabilities, and analyzed using the rough set theory to derive a set of design rules for design concept analysis. The approach embodies a technique for handling attributes with unavailable information, which is a frequent occurrence in design. This paper presents details of the proposed approach, the novel technique, and a case study.     

PWR annulus thermal hydraulics important to analysis of pressurized thermal shock

Analysis of plume mixing in the annulus of a pressurized water reactor (PWR) are presented. The plume mixing analysis is based on a simple two-dimensional model that accounts for the surrounding flow and confinement. A correlation for entrainment is presented and comparison with experiment is made.Mixed convection resulting from downflow between parallel heated plates is studied experimentally. The experimental system used to obtain the data is described with the scaling rationale for choosing the working fluid. Heat transfer results are presented in terms of a Nusselt number and a correlation is given. Results show an enhancement in heat transfer with increasing Gr_Dh/Re_Dh² due to an increase in turbulence intensity associated with the buoyant wall layer. The correlation obtained for Gr_Dh/Re_Dh² < 2.29 was found to be Nu_Dh/Nu_Dh,0 = 1 + 2.93 (Gr_Dh/Re_Dh²)^0.54, where where Nu_Dh,0 is given by the Dittus-Boelter correlation. Use of this correlation for Gr_Dh/Re_Dh² > 2.29 is not recommended due to an observed flow bifurcation in this neighborhood.     

CTLA4-Linked Autoimmunity in the Pathogenesis of Endometriosis and Related Infertility: A Systematic Review

Several studies, although with conflicting results, have sought to determine the concentration of soluble CTLA4 antigens in peripheral blood plasma and peritoneal fluid in patients with endometriosis-related infertility. A systematic review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) through a search of the following databases: MEDLINE, EMBASE, Global Health, The Cochrane Library, Health Technology Assessment Database and Web of Science, and Clinical Trials research register. We included observational or prospective human and animal studies with any features related to endometriosis and/or infertility studies involving CTLA4-related pathogenesis published in English. The results of studies in which the size and characteristics of the observed groups were not stated were excluded. From the initial pool of 73 publications identified and screened, we finally included 5 articles to summarize the most recent knowledge about CTLA4-linked autoimmunity in the pathogenesis of endometriosis and related infertility. Evidence from clinical studies shows that CTLA4-based autoimmunity is involved in the maintenance of chronic inflammation in the peritoneal environment, with pre-clinical evidence of anti-CTLA antibodies as a potential novel target therapy for endometriosis. However, CTLA4 gene analyses do not support findings of CTLA4-linked autoimmunity as a primary determinant of the pathogenesis of endometriosis. These findings underlie the role of complex interactions within the family of immune checkpoint molecules involved. Further studies are needed to investigate the clinical relevance of anti-CTLA target therapy, taking into account the potential adverse events and repercussions of novel immunologic therapy modalities. However, with the general scarcity of studies investigating this topic, the clinical importance of CTLA4 autoimmunity still remains unclear.     

Red and Blue Light Differently Influence Actinidia chinensis Performance and Its Interaction with Pseudomonas syringae pv. Actinidiae

Light composition modulates plant growth and defenses, thus influencing plant–pathogen interactions. We investigated the effects of different light-emitting diode (LED) red (R) (665 nm) and blue (B) (470 nm) light combinations on Actinidia chinensis performance by evaluating biometric parameters, chlorophyll a fluorescence, gas exchange and photosynthesis-related gene expression. Moreover, the influence of light on the infection by Pseudomonas syringae pv. actinidiae (Psa), the etiological agent of bacterial canker of kiwifruit, was investigated. Our study shows that 50%R–50%B (50R) and 25%R–75%B (25R) lead to the highest PSII efficiency and photosynthetic rate, but are the least effective in controlling the endophytic colonization of the host by Psa. Monochromatic red light severely reduced ΦPSII, ETR, Pn, TSS and photosynthesis-related genes expression, and both monochromatic lights lead to a reduction of DW and pigments content. Monochromatic blue light was the only treatment significantly reducing disease symptoms but did not reduce bacterial endophytic population. Our results suggest that monochromatic blue light reduces infection primarily by modulating Psa virulence more than host plant defenses.     

Differences in Synovial Cytokine Profile Associated with Long-Term Clinical Outcomes in Patients with Knee Osteoarthritis Undergoing Corrective Osteotomy with Platelet-Rich Plasma or Stromal Vascular Fraction Post-Treatments

Functional outcomes and synovial fluid (SF) cytokine concentrations in response to platelet-rich plasma (PRP) or stromal vascular fraction (SVF) post-treatments following open wedge high tibial osteotomy (HTO) in 20 patients with knee osteoarthritis (OA) were examined. Six weeks after surgery, the knees of 10 patients were injected with autologous PRP (PRP subgroup), while another 10 patients were injected with autologous SVF (SVF subgroup) and monitored for 1.5 years. Pain assessment (VAS score) and functional activity (KOOS, KSS, Outerbridge, and Koshino scores) were applied. PRP subgroup performed better compared with the SVF subgroup according to KOOS, KSS, and VAS scores, while the SVF subgroup demonstrated better results according to Outerbridge and Koshino testing and produced more pronounced cartilage regeneration in the medial condyle and slowed down cartilage destruction in its lateral counterpart. SF was collected before and one week after PRP or SVF injections and tested for concentrations of 41 cytokines (Multiplex Assay). In the PRP subgroup, a significant decrease in IL-6 and CXCL10 synovial concentrations was accompanied by an increase in IL-15, sCD40L, and PDGF-AB/BB amounts. The SVF subgroup demonstrated a significant decrease in synovial TNFα, FLT-3L, MIP-1β, RANTES, and VEGF concentrations while SF concentrations of MCP-1 and FGF2 increased. Both post-treatments have a potential for increased tissue regeneration, presumably due to the downregulation of inflammation and augmentation of synovial growth factor concentrations.     

Fenbendazole Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice via Suppression of Fibroblast-to-Myofibroblast Differentiation

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and fatal interstitial lung disease with unknown etiology. Despite substantial progress in understanding the pathogenesis of pulmonary fibrosis and drug development, there is still no cure for this devastating disease. Fenbendazole (FBZ) is a benzimidazole compound that is widely used as an anthelmintic agent and recent studies have expanded the scope of its pharmacological effects and application prospect. This study demonstrated that FBZ treatment blunted bleomycin-induced lung fibrosis in mice. In vitro studies showed that FBZ inhibited the proliferation and migration of human embryo lung fibroblasts. Further studies showed that FBZ significantly inhibited glucose consumption, moderated glycolytic metabolism in fibroblasts, thus activated adenosine monophosphate-activated protein kinase (AMPK), and reduced the activation of the mammalian target of rapamycin (mTOR) pathway, thereby inhibiting transforming growth factor-β (TGF-β1)-induced fibroblast-to-myofibroblast differentiation and collagen synthesis. In summary, our data suggested that FBZ has potential as a novel treatment for pulmonary fibrosis.