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31.
New labels and assay techniques are needed to improve the sensitivity and quantitativeness of point-of-care immunotesting while sustaining the rapidity and ease of use of the assays. We synthesized a novel, intrinsically fluorescent nonadentate europium chelate with two chromophores and hydrophilic alpha-galactose side groups. The chelate is highly fluorescent, soluble in water, and provides effective shielding of Eu from water. The performance of the nonadentate chelate was compared with a heptadentate chelate in a dry reagent immunoassay for human chorionic gonadotropin (hCG). After 15-min incubation and washing, time-resolved fluorescence was measured directly from a wet or dried well surface. Contrary to the heptadentate label, the effect of aqueous quenching on the nonadentate label was found to be insignificant, with calculated analytical detection limits (background + 3 SD) of 0.9 and 0.7 IU/L hCG for wet and dry measurements, respectively, and a linear range up to 5000 IU/L. The CVs for the new label were <8% at the cutoff of 25 IU/L and above in both whole blood and plasma. The novel nonadentate label facilitates short turnaround times and simple instrumentation due to the absence of all signal development steps, at the same time retaining an excellent immunoassay performance. 相似文献
32.
Tarvainen MP Hiltunen JK Ranta-aho PO Karjalainen PA 《IEEE transactions on bio-medical engineering》2004,51(3):516-524
An adaptive spectrum estimation method for nonstationary electroencephalogram by means of time-varying autoregressive moving average modeling is presented. The time-varying parameter estimation problem is solved by Kalman filtering along with a fixed-interval smoothing procedure. Kalman filter is an optimal filter in the mean square sense and it is a generalization of other adaptive filters such as recursive least squares or least mean square. Furthermore, by using the smoother the unavoidable tracking lag of adaptive filters can be avoided. Due to the properties of Kalman filter and benefits of the smoothing the time-frequency resolution of the presented Kalman smoother spectra is extremely high. The presented approach is applied to estimation of event-related synchronization/desynchronization (ERS/ERD) dynamics of occipital alpha rhythm measured from three healthy subjects. With the Kalman smoother approach detailed spectral information can be extracted from single ERS/ERD samples. 相似文献
33.
Jaana Pinnick Andrew Riddick Robert McLaverty Garry Baker 《New Review of Information Networking》2018,23(1-2):99-110
The British Geological Survey (BGS) is responsible for managing a major geoscience data archive on behalf of the UK Nuclear Decommissioning Authority (NDA). Much of this geological data was captured during the 1990s and early 2000s using now obsolete software and data formats. This data asset remains an important resource for the NDA and the wider scientific community. The NDA wishes to ensure the data remain accessible and usable for many decades into the future. BGS has been working closely with Radioactive Waste Management (RWM), a wholly owned subsidiary of the NDA, on a program of data management and digital continuity measures to ensure the long-term usability of the data. This article describes some of the challenges and outlines the approaches we have taken to address these issues. 相似文献
34.
V??n?nen Ari; Buunk Bram P.; Kivim?ki Mika; Pentti Jaana; Vahtera Jussi 《Canadian Metallurgical Quarterly》2005,89(2):176
The authors examined esteem enhancement theory and equity theory in explaining the long-term health status of men (n = 178) and women (n = 575). Baseline characteristics were measured in 1990-1993, intimate reciprocity in 1993, and recorded sickness absences for a 9-year period during 1993-2001. Among women, giving more support than receiving in intimate relationships positively affected subsequent health status, as indicated by fewer sickness absences. Among men, receiving more support than giving predicted fewer absences. These effects remained even after significant covariates were included in the model. Thus, women seem to benefit from giving support in intimate relationships, and men seem to benefit from receiving support in intimate relationships. The findings on women are in line with the esteem enhancement theory. No support was obtained for the equity theory. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
35.
Jaana Jarva Timo Tarvainen Mikael Eklund 《The Science of the total environment》2010,408(20):4385-4395
In Finland, a Government Decree on the Assessment of Soil Contamination and Remediation Needs has generated a need for reliable and readily accessible data on geochemical baseline concentrations in Finnish soils. According to the Decree, baseline concentrations, referring both to the natural geological background concentrations and the diffuse anthropogenic input of substances, shall be taken into account in the soil contamination assessment process. This baseline information is provided in a national geochemical baseline database, TAPIR, that is publicly available via the Internet.Geochemical provinces with elevated baseline concentrations were delineated to provide regional geochemical baseline values. The nationwide geochemical datasets were used to divide Finland into geochemical provinces. Several metals (Co, Cr, Cu, Ni, V, and Zn) showed anomalous concentrations in seven regions that were defined as metal provinces. Arsenic did not follow a similar distribution to any other elements, and four arsenic provinces were separately determined. Nationwide geochemical datasets were not available for some other important elements such as Cd and Pb. Although these elements are included in the TAPIR system, their distribution does not necessarily follow the ones pre-defined for metal and arsenic provinces.Regional geochemical baseline values, presented as upper limit of geochemical variation within the region, can be used as trigger values to assess potential soil contamination. Baseline values have also been used to determine upper and lower guideline values that must be taken into account as a tool in basic risk assessment. If regional geochemical baseline values are available, the national guideline values prescribed in the Decree based on ecological risks can be modified accordingly.The national geochemical baseline database provides scientifically sound, easily accessible and generally accepted information on the baseline values, and it can be used in various environmental applications. 相似文献
36.
The SEB1/SBH1 and the SSO genes encode components of the protein secretory machinery functioning at the opposite ends, ER translocation and exocytosis, respectively, of the secretory pathway in Saccharomyces cerevisiae. Overexpression of these genes can rescue temperature-sensitive (ts) growth defect of many sec mutants impaired in protein secretion. Furthermore, their overexpression in wild-type yeast enhances production of secreted proteins in S. cerevisiae, which suggests that they may be rate-limiting factors in this process. Here we report isolation of Kluyveromyces lactis homologues of these genes. KlSSO1 and KlSEB1 were isolated as clones capable of rescuing growth of ts sso2-1 and seb1Delta seb2Delta sem1Delta strains, respectively, at the restrictive temperature. The encoded Kluyveromyces proteins are up to 70% identical with the S. cerevisiae homologues at the amino acid level and can functionally replace them. Interestingly, KlSSO1 and KlSEB1 show similar enhancing effect on production of a secreted protein as the SSO and SEB1 genes of S. cerevisiae when overexpressed. In accordance with the high homology level of the secretory pathway proteins in different yeast species, the polyclonal antibodies raised against S. cerevisiae Seb1p, Sso2p and Sec4p can detect homologous proteins in cell lysates of K. lactis and Pichia pastoris, the latter also in Candida utilis. The GenBank Accession Nos are AF307983 (K. lactis SSO1) and AF318314 (K. lactis SEB1). 相似文献
37.
38.
While virtual community research has contributed to the understanding of the virtual community sustainability, a need for a systematic model exists. In this paper, we propose a model of sustainable virtual communities based on the sustainability properties of animal colonies in nature. The premise of our model is that if we manage to replicate the sustainability properties of colonial systems in virtual communities, they can become longer lasting. We also present the results of an exploratory case study. The study shows that colonial properties form a construct that predicts member perceptions of their community being sustainable. While our primary objective is the conceptual introduction of the colonial principles of sustainability, the case study provides some early indications that these principles may help design systems that can bind virtual communities more permanently.
相似文献
Michael S. ParksEmail: Phone: +1-713-7344729 |
39.
Jaana M. Kusnetsov Pertti J. Martikainen Hannele R. Jousimies-Somer Marja-Liisa Visnen Arto I. Tulkki Henry E. Ahonen Aino I. Nevalainen 《Water research》1993,27(1)
The occurrence of Legionella in water of 30 cooling tower systems was studied, as well as the relationship of its occurrence with the physical, chemical and microbiological characteristics of the waters. The samples were concentrated by membrane filtration and one part of the concentrate was acid washed. Three types of culture media, MWY, CCVC and BCYEα were used. Fourteen (47%) of the cooling systems were Legionella-positive. Numbers of Legionella in the positive systems varied between 50 and 490,000 cfu/l. Legionella pneumophila was the dominant isolate, and the serogroups 6 and 1 were the commonest ones. There was no statistically significant difference in mean water temperature between the Legionella-positive (27°C) and -negative (24°C) systems. The total number of bacteria (AODC) was lower in the Legionella-positive than in the negative systems. Also the nutrient concentrations were generally lower in the Legionella-positive cooling systems. The results suggest that different factors regulate the occurrence of Legionella than that of overall bacterial populations in the cooling tower systems. 相似文献
40.
Tirri T Rich J Wolke J Seppälä J Yli-Urpo A Närhi TO 《Journal of materials science. Materials in medicine》2008,19(8):2919-2923
The aim of this study was to investigate in vitro bioactivity of different thermoplastic biodegradable barrier membranes. Three experimental GBR membranes were fabricated using Poly(epsilon-caprolactone-co-D: ,L-lactide) P(CL/DL-LA) and particulate bioactive glass S53P4 (BAG; granule size 90-315 microm): (A) composite membrane with 60-wt.% of BAG, (B) membrane coated with BAG; and (C) copolymer membrane without BAG. Membranes were immersed in simulated body fluid (SBF), and their surfaces were characterized with SEM, XRD and EDS after 6 and 12 h and after 1, 3, 5, 7, and 14 days. Calcium phosphate (Ca-P) surface formation was observed on both composite membranes (A and B) but not on the copolymer membrane without bioactive glass (C). The Ca-P precipitation appeared to be initiated on the bioactive glass followed by growth of the layer along the polymer surface. In 6-12 h ion dissolution of the bioactive glass led to formation of the silica rich layer on the surface of the exposed glass granules on composite membrane B whereas only small amounts of silica was observed on the polymer surface of the composite membrane A. At 24 h nucleation of Ca-P precipitation was observed, and by 3-5 days membrane surface was covered with a uniform Ca-P layer transforming from amorphous to low crystalline structure. At 7 days composition and structure of the apatite surface resembled the apatite in bone. Once nucleated, the surface topography seemed to have significant effect on the growth of the apatite layer. 相似文献