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排序方式: 共有583条查询结果,搜索用时 15 毫秒
191.
Jeonghyun Kim Anthony Banks Huanyu Cheng Zhaoqian Xie Sheng Xu Kyung‐In Jang Jung Woo Lee Zhuangjian Liu Philipp Gutruf Xian Huang Pinghung Wei Fei Liu Kan Li Mitul Dalal Roozbeh Ghaffari Xue Feng Yonggang Huang Sanjay Gupta Ungyu Paik John A. Rogers 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(8):906-912
192.
Myeongwoo Kang Woohyun Park Sangcheol Na Sang‐Min Paik Hyunjae Lee Jae Woo Park Ho‐Young Kim Noo Li Jeon 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(23):2789-2797
Soft lithography and other techniques have been developed to investigate biological and chemical phenomena as an alternative to photolithography‐based patterning methods that have compatibility problems. Here, a simple approach for nonlithographic patterning of liquids and gels inside microchannels is described. Using a design that incorporates strategically placed microstructures inside the channel, microliquids or gels can be spontaneously trapped and patterned when the channel is drained. The ability to form microscale patterns inside microfluidic channels using simple fluid drain motion offers many advantages. This method is geometrically analyzed based on hydrodynamics and verified with simulation and experiments. Various materials (i.e., water, hydrogels, and other liquids) are successfully patterned with complex shapes that are isolated from each other. Multiple cell types are patterned within the gels. Capillarity guided patterning (CGP) is fast, simple, and robust. It is not limited by pattern shape, size, cell type, and material. In a simple three‐step process, a 3D cancer model that mimics cell–cell and cell–extracellular matrix interactions is engineered. The simplicity and robustness of the CGP will be attractive for developing novel in vitro models of organ‐on‐a‐chip and other biological experimental platforms amenable to long‐term observation of dynamic events using advanced imaging and analytical techniques. 相似文献
193.
Choi SU Paik HD Lee SC Nihira T Hwang YI 《Journal of Bioscience and Bioengineering》2004,98(2):132-135
Human lysozyme (HLY) was successfully produced by Saccharomyces cerevisiae with a plasmid (pHK501) containing a synthetic HLY gene connected with a native secretion-signal sequence under control of the promoter for the glyceraldehydes-3-phosphate dehydrogenase gene. For large-scale production, batch fermentation was adopted with 1 l SD (Leu(-)) minimal medium in a 2-l jar fermentor. While pH-uncontrolled fermentation gave 42.3 units/ml of HLY over a period of 72-h cultivation, HLY production increased to 74.5 units/ml by performing pH-controlled fermentation, although a longer fermentation time was needed for maximal HLY production. A pH-shift strategy was designed in this study, resulting in higher HLY production and a shorter fermentation time. 相似文献
194.
195.
C Richard Hofstetter Melbourne F Hovell Jooeun Lee Joy Zakarian Haeryun Park Hee-Young Paik Veronica Irvin 《Nicotine & tobacco research》2004,6(3):481-489
This study presents population estimates of cigarette use among adults of Korean descent residing in California. Data were drawn from telephone interviews with adults (N=2,830) developed from a random sampling of listed persons in California with Korean surnames. A total of 86% of attempted interviews were completed, and 85% of the interviews were conducted in Korean. Less acculturated men and more acculturated women reported higher present and predicted future rates of smoking after multivariate statistical controls were applied. Sharply divergent rates of cigarette use were found between the genders. Although men did not smoke their first cigarette or smoke cigarettes regularly earlier than women, both groups began smoking later than adults born in the United States. To be effective, tobacco intervention efforts must be tailored specifically to cultures of each minority. Results suggest that acculturation processes influence tobacco use differentially by gender, and future research is needed to identify the implications of processes in tobacco initiation and cessation. 相似文献
196.
Yongwon Choi Jiwon Shin Kyung-lim Suk Young Soon Kim Il Kim Kyung-Wook Paik 《Journal of Electronic Materials》2014,43(11):4214-4223
Much research has been carried out to realize through-silicon via (TSV) technology for three-dimensional (3D) chip stacking packaging. A vertical chip interconnection method using Cu/Sn-Ag bumps and nonconductive films (NCFs) is one of the most promising approaches for 3D TSV vertical interconnection. In this work, the relationship between the viscosity of pre-applied NCFs and loading forces was investigated to predict the gap change between a TSV chip and a substrate chip. Existing theories of squeeze flow are adapted to predict the gap change of a real TSV chip and a substrate chip during TSV bonding using a simplified model. The real gaps measured during bonding of test dies were matched to check the validity of the prediction model. Considering the thixotropy of NCFs, the prediction well matched the real gap changes between bumped TSV chips and substrate chips during bonding. 相似文献
197.
File entropy is one of the major indicators of crypto-ransomware because the encryption by ransomware increases the randomness of file contents. However, entropy-based ransomware detection has certain limitations; for example, when distinguishing ransomware-encrypted files from normal files with inherently high-level entropy, misclassification is very possible. In addition, the entropy evaluation cost for an entire file renders entropy-based detection impractical for large files. In this paper, we propose two indicators based on byte frequency for use in ransomware detection; these are termed EntropySA and DistSA, and both consider the interesting characteristics of certain file subareas termed "sample areas' (SAs). For an encrypted file, both the sampled area and the whole file exhibit high-level randomness, but for a plain file, the sampled area embeds informative structures such as a file header and thus exhibits relatively low-level randomness even though the entire file exhibits high-level randomness. EntropySA and DistSA use "byte frequency" and a variation of byte frequency, respectively, derived from sampled areas. Both indicators cause less overhead than other entropy-based detection methods, as experimentally proven using realistic ransomware samples. To evaluate the effectiveness and feasibility of our indicators, we also employ three expensive but elaborate classification models (neural network, support vector machine and threshold-based approaches). Using these models, our experimental indicators yielded an average F1-measure of 0.994 and an average detection rate of 99.46% for file encryption attacks by realistic ransomware samples. 相似文献
198.
RL Sacco M Elkind B Boden-Albala IF Lin DE Kargman WA Hauser S Shea MC Paik 《Canadian Metallurgical Quarterly》1999,281(1):53-60
Experiments were designed to investigate the influence of estrous cycle and gender of the rat on the effects of a gamma-aminobutyric acid type A (GABA(A)) receptor active neurosteroid, 3alpha-hydroxy-5alpha-pregnan-20-one (allopregnanolone), the benzodiazepine, triazolam, and a GABA(A) receptor antagonistic neurosteroid, delta5-androsten-3beta-ol-17-one sulfate (dehydroepiandrosterone sulfate), on food intake and elevated plus-maze learning behaviors. Allopregnanolone (0.25 mg/kg, s.c.) and triazolam (0.25 mg/kg, i.p.) produced a hyperphagic effect, while dehydroepiandrosterone sulfate (5 mg/kg, s.c.) elicited an anorectic effect. However, allopregnanolone was more potent in diestrous females, whereas triazolam exhibited significantly higher hyperphagic potency in estrus females. The extent of anorexia following dehydroepiandrosterone sulfate was alike in male and female rats. The triazolam- and allopregnanolone-induced hyperphagic effect was blocked by bicuculline (1 mg/kg, i.p.), a selective GABA(A) receptor antagonist. In contrast to triazolam, the hyperphagic effect of allopregnanolone was insensitive to flumazenil (5 mg/kg, i.p.), a benzodiazepine antagonist. Vehicle-treated diestrous rats displayed moderately higher latencies in the elevated plus-maze learning task than estrus or proestrus females. Although allopregnanolone and triazolam elicited equipotent learning deficits in plus-maze learning in male and female rats, the magnitude of impairment-induced by triazolam was significantly higher in diestrous females than proestrus females. Dehydroepiandrosterone sulfate enhanced memory performance only in male rats. Although the use of the elevated plus-maze as a learning paradigm with benzodiazepines and neurosteroids may be sensitive to changes in anxiety, the differential data suggest that neurosteroid-induced effects are at least partly specific to learning behavior. These results confirm the role of estrous cycle and sex of rats in modifying the potency of neurosteroids and benzodiazepines on food consumption and learning and memory processes. 相似文献
199.
All plastics can be divided into two groups called thermoplastics and thermosets, and each group exhibits very different behaviors. Unlike thermosets, thermoplastics are empirically known by the viscoelastic behavior of the thermoplastic layers; the stress generated by building subsequent layers can be significantly relieved in a multilayer structure. In this study, we quantitatively investigated the stress relaxation effect of thermoplastics in lamination-based multilayer substrates. As an increasing number of layers were laminated using either thermoplastic or thermoset adhesives, the maximum bow values were measured layer-by-layer using a laser profilometry during thermal cycling. When analyzed through an analytical model, the measured thermal behavior of thermoplastics exhibited as much as 70% stress relaxation in the multilayer structures, and highly contrasted with that of thermoset adhesives. The result provides an important design guideline that thermoplastics can be used as a stress relaxation layer in lamination-based multilayer substrates 相似文献
200.
For design and strength assessment of various types of structures such as ships, offshore platforms, land-based structures, and aerospace structures, it has been realized that ultimate limit states (or ultimate strength) are much better basis than the allowable working stresses. Within the framework of ultimate limit state design and strength assessment, the primary task is to determine the level of imposed actions which causes the ultimate limit states. The aim of the present study is to develop some useful insights on nonlinear finite element method application for ultimate limit state assessment of steel stiffened-plate structures subject to combined biaxial compression and lateral pressure actions. As an illustrative example, outer bottom stiffened-plate structures of 100,000 ton class double-hull oil tankers are considered. The present study consists of two parts; Part I deals with plate elements surrounded by stiffeners or support members, and Part II treats stiffened panels, where some important factors of influence such as structural dimensions, initial imperfections, loading types and computational techniques in association with ultimate limit states are studied. For ultimate limit state computations, ANSYS nonlinear finite element method together with ALPS/ULSAP semi-analytical method is employed. Important insights developed from the present study are documented. 相似文献