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31.
The production of zeolite granules varies in the amounts of binder content and different drying/activation temperatures. Here, the influence of moisture content and cyclic moisture loading and unloading on the mechanical properties like the strength of three different types of zeolite granules has been studied by uniaxial compression tests. The force‐displacement curves and scanning electron microscopy pictures of the granule and fracture surfaces show that with increasing number of moistening‐drying cycles, a continuous decrease in the strength of granules take place due to non‐regenerative damage of the structure. 相似文献
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Christopher D. Sinigalliano Jared S. Ervin Laurie C. Van De Werfhorst Brian D. Badgley Elisenda Ballesté Jakob Bartkowiak Alexandria B. Boehm Muruleedhara Byappanahalli Kelly D. Goodwin Michèle Gourmelon John Griffith Patricia A. Holden Jenny Jay Blythe Layton Cheonghoon Lee Jiyoung Lee Wim G. Meijer Rachel Noble Meredith Raith Hodon Ryu Michael J. Sadowsky Alexander Schriewer Dan Wang David Wanless Richard Whitman Stefan Wuertz Jorge W. Santo Domingo 《Water research》2013
Here we report results from a multi-laboratory (n = 11) evaluation of four different PCR methods targeting the 16S rRNA gene of Catellicoccus marimammalium originally developed to detect gull fecal contamination in coastal environments. The methods included a conventional end-point PCR method, a SYBR® Green qPCR method, and two TaqMan® qPCR methods. Different techniques for data normalization and analysis were tested. Data analysis methods had a pronounced impact on assay sensitivity and specificity calculations. Across-laboratory standardization of metrics including the lower limit of quantification (LLOQ), target detected but not quantifiable (DNQ), and target not detected (ND) significantly improved results compared to results submitted by individual laboratories prior to definition standardization. The unit of measure used for data normalization also had a pronounced effect on measured assay performance. Data normalization to DNA mass improved quantitative method performance as compared to enterococcus normalization. The MST methods tested here were originally designed for gulls but were found in this study to also detect feces from other birds, particularly feces composited from pigeons. Sequencing efforts showed that some pigeon feces from California contained sequences similar to C. marimammalium found in gull feces. These data suggest that the prevalence, geographic scope, and ecology of C. marimammalium in host birds other than gulls require further investigation. This study represents an important first step in the multi-laboratory assessment of these methods and highlights the need to broaden and standardize additional evaluations, including environmentally relevant target concentrations in ambient waters from diverse geographic regions. 相似文献
35.
Steven C. Cermak Jakob W. Bredsguard Robert O. Dunn Travis Thompson Kati A. Feken Katie L. Roth James A. Kenar Terry A. Isbell Rex E. Murray 《Journal of the American Oil Chemists' Society》2014,91(12):2101-2109
A series of 26 different antioxidants and commercial antioxidant packages designed for petroleum‐based materials, containing both natural and synthetic‐based materials, were evaluated with dimeric coconut‐oleic estolide 2‐ethylhexyl ester (2‐EH), a bio‐based material. The different antioxidants were categorized into different classes of phenolic, aminic, and blended/others materials. The oxidation onset temperatures (OT) using non‐isothermal pressurized differential scanning calorimetry (PDSC) were measured and recorded under previously reported standard conditions. The aminic series gave the best resistance to oxidation as defined by the PDSC method with OT of 246.6 and 244.7 °C for the best two performers, which was a 38 °C improvement over the uninhibited or unformulated dimer estolide material. The phenolic series, containing most of the naturally occurring antioxidants, was the least successful formulation package for the dimer estolide. The blended/other materials, which were specifically designed for petroleum‐based lubricants, did not have the best OT, since the estolides and other bio‐based materials interact differently than their petroleum counterparts. A number of potential antioxidants have been identified as useful additives for the estolides esters. The OT of the estolide and formulated materials correlated well with other bio‐based materials such as biodiesel. 相似文献
36.
Hill Benjamin Santner Jakob Spiegel Heide Puschenreiter Markus Wenzel Walter W. 《Nutrient Cycling in Agroecosystems》2021,121(2-3):227-240
Nutrient Cycling in Agroecosystems - Soil P testing has been widely used to predict crop yields, P uptake, and fertilizer demands in agriculture. Diffusive gradients in thin films (DGT) provides a... 相似文献
37.
Alexander Schriewer Kelly D. Goodwin Christopher D. Sinigalliano Annie M. Cox David Wanless Jakob Bartkowiak Darcy L. Ebentier Kaitlyn T. Hanley Jared Ervin Louise A. Deering Orin C. Shanks Lindsay A. Peed Wim G. Meijer John F. Griffith Jorge SantoDomingo Jennifer A. Jay Patricia A. Holden Stefan Wuertz 《Water research》2013
The contribution of fecal pollution from dogs in urbanized areas can be significant and is an often underestimated problem. Microbial source tracking methods (MST) utilizing quantitative PCR of dog-associated gene sequences encoding 16S rRNA of Bacteroidales are a useful tool to estimate these contributions. However, data about the performance of available assays are scarce. The results of a multi-laboratory study testing two assays for the determination of dog-associated Bacteroidales (DogBact and BacCan-UCD) on 64 single and mixed fecal source samples created from pooled fecal samples collected in California are presented here. Standardization of qPCR data treatment lowered inter-laboratory variability of sensitivity and specificity results. Both assays exhibited 100% sensitivity. Normalization methods are presented that eliminated random and confirmed non-target responses. The combination of standardized qPCR data treatment, use of normalization via a non-target specific Bacteroidales assay (GenBac3), and application of threshold criteria improved the calculated specificity significantly for both assays. Such measures would reasonably improve MST data interpretation not only for canine-associated assays, but for all qPCR assays used in identifying and monitoring fecal pollution in the environment. 相似文献
38.
Efficient removal of phthalate esters (PE) in wastewater treatment plants (WWTP) is becoming an increasing priority in many countries. In this study, we examined the fate of dimethyl phthalate (DMP), dibutyl phthalate (DBP), butylbenzyl phthalate (BBP), and di-(2-ethylhexyl) phthalate (DEHP) in a full scale activated sludge WWTP with biological removal of nitrogen and phosphorus. The mean concentrations of DMP, DBP, BBP, and DEHP at the WWTP inlet were 1.9, 20.5, 37.9, and 71.9 μg/L, respectively. Less than 0.1%, 42%, 35%, and 96% of DMP, DBP, BBP, and DEHP was associated with suspended solids, respectively. The overall microbial degradation of DMP, DBP, BBP, and DEHP in the WWTP was estimated to be 93%, 91%, 90%, and 81%, respectively. Seven to nine percent of the incoming PE were recovered in the WWTP effluent. Factors affecting microbial degradation of DEHP in activated sludge were studied using [U-14C-ring] DEHP as tracer. First order rate coefficients for aerobic DEHP degradation were 1.0×10−2, 1.4×10−2, and 1.3×10−3 at 20, 32, and 43 °C, respectively. Aerobic degradation rates decreased dramatically under aerobic thermophilic conditions (<0.1×10−2 h−1 at 60 °C). The degradation rate under anoxic denitrifying conditions was 0.3×10−2 h−1, whereas the rate under alternating conditions (aerobic-anoxic) was 0.8×10−2 h−1. Aerobic DEHP degradation in activated sludge samples was stimulated 5-9 times by addition of a phthalate degrading bacterium. The phthalate degrading bacterium was isolated from activated sludge, and maintained a capacity for DEHP degradation while growing on vegetable oil. Collectively, the results of the study identified several controls of microbial PE degradation in activated sludge. These controls may be considered to enhance PE degradation in activated sludge WWTP with biological removal of nitrogen and phosphorus. 相似文献
39.
Composition of bacterial and archaeal communities in freshwater sediments with different contamination levels (Lake Geneva, Switzerland) 总被引:6,自引:0,他引:6
The aim of this study was to compare the composition of bacterial and archaeal communities in contaminated sediments (Vidy Bay) with uncontaminated sediments (Ouchy area) of Lake Geneva using 16S rRNA clone libraries. Sediments of both sites were analysed for physicochemical characteristics including porewater composition, organic carbon, and heavy metals. Results show high concentrations of contaminants in sediments from Vidy. Particularly, high contents of fresh organic matter and nutrients led to intense mineralisation, which was dominated by sulphate-reduction and methanogenesis. The bacterial diversity in Vidy sediments was significantly different from the communities in the uncontaminated sediments. Phylogenetic analysis revealed a large proportion of Betaproteobacteria clones in Vidy sediments related to Dechloromonas sp., a group of dechlorinating and contaminant degrading bacteria. Deltaproteobacteria, including clones related to sulphate-reducing bacteria and Fe(III)-reducing bacteria (Geobacter sp.) were also more abundant in the contaminated sediments. The archaeal communities consisted essentially of methanogenic Euryarchaeota, mainly found in the contaminated sediments rich in organic matter. Multiple factor analysis revealed that the microbial community composition and the environmental variables were correlated at the two sites, which suggests that in addition to environmental parameters, pollution may be one of the factors affecting microbial community structure. 相似文献
40.
Tingting Gu Jesper Just Katrine Tang Stenz Yan Yan Peter Sieljacks Jakob Wang Thomas Skjaerlund Groennebaek Jesper Emil Jakobsgaard Emil Rindom Jon Herskind Anders Gravholt Thomas Ravn Lassen Mathias Jrgensen Rikke Bk Eugenio Gutirrez-Jimnez Nina Kerting Iversen Peter Mondrup Rasmussen Jens Randel Nyengaard Malene Mller Jrgensen Frank de Paoli Hans Erik Btker Jrgen Kjems Kristian Vissing Kim Ryun Drasbek 《International journal of molecular sciences》2022,23(6)
Ischemic conditioning and exercise have been suggested for protecting against brain ischemia-reperfusion injury. However, the endogenous protective mechanisms stimulated by these interventions remain unclear. Here, in a comprehensive translational study, we investigated the protective role of extracellular vesicles (EVs) released after remote ischemic conditioning (RIC), blood flow restricted resistance exercise (BFRRE), or high-load resistance exercise (HLRE). Blood samples were collected from human participants before and at serial time points after intervention. RIC and BFRRE plasma EVs released early after stimulation improved viability of endothelial cells subjected to oxygen-glucose deprivation. Furthermore, post-RIC EVs accumulated in the ischemic area of a stroke mouse model, and a mean decrease in infarct volume was observed for post-RIC EVs, although not reaching statistical significance. Thus, circulating EVs induced by RIC and BFRRE can mediate protection, but the in vivo and translational effects of conditioned EVs require further experimental verification. 相似文献