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Ivan Rehor Jitka Slegerova Jan Kucka Vladimir Proks Vladimira Petrakova Marie‐Pierre Adam François Treussart Stuart Turner Sara Bals Pavel Sacha Miroslav Ledvina Amy M. Wen Nicole F. Steinmetz Petr Cigler 《Small (Weinheim an der Bergstrasse, Germany)》2014,10(6):1106-1115
High pressure high temperature (HPHT) nanodiamonds (NDs) represent extremely promising materials for construction of fluorescent nanoprobes and nanosensors. However, some properties of bare NDs limit their direct use in these applications: they precipitate in biological solutions, only a limited set of bio‐orthogonal conjugation techniques is available and the accessible material is greatly polydisperse in shape. In this work, we encapsulate bright 30‐nm fluorescent nanodiamonds (FNDs) in 10–20‐nm thick translucent (i.e., not altering FND fluorescence) silica shells, yielding monodisperse near‐spherical particles of mean diameter 66 nm. High yield modification of the shells with PEG chains stabilizes the particles in ionic solutions, making them applicable in biological environments. We further modify the opposite ends of PEG chains with fluorescent dyes or vectoring peptide using click chemistry. High conversion of this bio‐orthogonal coupling yielded circa 2000 dye or peptide molecules on a single FND. We demonstrate the superior properties of these particles by in vitro interaction with human prostate cancer cells: while bare nanodiamonds strongly aggregate in the buffer and adsorb onto the cell membrane, the shell encapsulated NDs do not adsorb nonspecifically and they penetrate inside the cells. 相似文献
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Identification of Multiple Dityrosine Bonds in Materials Composed of the Drosophila Protein Ultrabithorax 下载免费PDF全文
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Jan Berger Evan J. Spadafora Pingo Mutombo Pavel Jelínek Martin Švec 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(30):3686-3693
A single atomic manipulation on the delta‐doped B:Si(111)‐()R30° surface using a low temperature dynamic atomic force microscopy based on the Kolibri sensor is investigated. Through a controlled vertical displacement of the probe, a single Si adatom in order to open a vacancy is removed. It is shown that this process is completely reversible, by accurately placing a Si atom back into the vacancy site. In addition, density functional theory simulations are carried out to understand the underlying mechanism of the atomic manipulation in detail. This process also rearranges the atoms at the tip apex, which can be effectively sharpened in this way. Such sharper tips allow for a deeper look into the Si adatom vacancy site. Namely, high‐resolution images of the vacancy showing subsurface Si dangling bond triplets, which surround the substitutional B dopant atom in the first bilayer, are achieved. 相似文献
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Jan Herrmann Jürgen KoreckMatthias Maess Lothar Gaul 《Mechanical Systems and Signal Processing》2011,25(3):981-990
The integration of a model for longitudinal hydroacoustic fluid damping in thin hydraulic pipes in 3D finite element models is presented in this paper. In order to perform quantitative prediction of the vibroacoustic behavior and resulting noise levels of such fluid-structure coupled system due to hydraulic excitation, an accurate frequency-dependent fluid damping model including friction effects near the pipe wall is required. This step is achieved by matching complex wave numbers from analytical derivation into a parameterized damped wave equation and consecutive translation into finite element modeling.Since the friction effect close to the pipe wall changes locally with the inner pipe radius, the fluid damping model is applied segment-wise in order to model the influence of cross-sectional discontinuity, such as orifices, on the oscillating pressure pulsations. A component synthesis approach, which uses pipe segments as substructures, allows a simple model generation and fast computation times.The numerical harmonic results are compared to experimental frequency response functions, which are performed on a hydraulic test bench driven by a dynamic pressure source in the kHz-range. 相似文献
958.
Marek Vochozka Anna Maroušková Jan Váchal Jarmila Straková 《Energy Sources, Part A: Recovery, Utilization, and Environmental Effects》2016,38(22):3332-3337
Steam-explosion pretreatment allows lowering of the natural resistance of plant material to subsequent biodegrading processes, such as anaerobic fermentation. This is achieved by a complex of cavitation forces that disintegrate rigid lignocellulose structures and liberate labile organic matter during a quick release of phytomass from a steam-pressurized reactor back to the atmospheric pressure. Hydrolyzing reactants or catalysts may increase the effect; however, the management of chemicals raises financial and environmental concerns. The current state of knowledge was reviewed to identify promising designs and reengineering that would enable subsequent transfer into the commercial scale in order to reverse the current trend of phytomass waste management – landfilling. In an effort to achieve profitability, it has been concluded that further development should focus on a deeper interconnection of the apparatus with associated technology units, reuse of waste heat from the subsequent biogas combustion, widening the range of processing parameters, reuse of nutrients, and utilization of the ballast organic matter. 相似文献
959.
ABSTRACTSuperheaters are high-temperature cross-flow heat exchangers. Steam flows inside the tubes, and the flue gas outside in a direction perpendicular to the axes of the tubes. However, they differ very substantially from the other heat exchangers operating at low temperatures. Superheaters are characterized by complex flow system and high tube walls temperature. Superheaters are among the most exposed to damaging pressure elements of steam boilers. Damage to the superheater causes about 40% of emergency shutdowns of boilers. The paper presents the boiler superheater model with distributed parameters, which is used to determine on-line the degree of superheater fouling by ash. 相似文献
960.
Pierre Pinson Henrik Aa. Nielsen Jan K. Møller Henrik Madsen George N. Kariniotakis 《风能》2007,10(6):497-516
Predictions of wind power production for horizons up to 48–72 h ahead comprise a highly valuable input to the methods for the daily management or trading of wind generation. Today, users of wind power predictions are not only provided with point predictions, which are estimates of the conditional expectation of the wind generation for each look‐ahead time, but also with uncertainty estimates given by probabilistic forecasts. In order to avoid assumptions on the shape of predictive distributions, these probabilistic predictions are produced from non‐parametric methods, and then take the form of a single or a set of quantile forecasts. The required and desirable properties of such probabilistic forecasts are defined and a framework for their evaluation is proposed. This framework is applied for evaluating the quality of two statistical methods producing full predictive distributions from point predictions of wind power. These distributions are defined by a number of quantile forecasts with nominal proportions spanning the unit interval. The relevance and interest of the introduced evaluation framework are discussed. Copyright © 2007 John Wiley & Sons, Ltd. 相似文献