Monitoring in real time with a microelectrode the release of reactive oxygen and nitrogen species by a single macrophage stimulated by its membrane mechanical depolarization

Macrophages are key cells of the immune system. During phagocytosis, the macrophage engulfs a foreign bacterium, virus, or particle into a vacuole, the phagosome, wherein oxidants are produced to neutralize and decompose the threatening element. These oxidants derive from in situ production of superoxide and nitric oxide by specific enzymes. However, the chemical nature and sequence of release of these compounds is far from being completely determined. The aim of the present work was to study the fundamental mechanism of oxidant release by macrophages at the level of a single cell, in real time and quantitatively. The tip of a microelectrode was positioned at a micrometric distance from a macrophage in a culture to measure oxidative-burst release by the cell when it was submitted to physical stimulation. The ensuing release of electroactive reactive oxygen and nitrogen species was detected by amperometry and the exact nature of the compounds was characterized through comparison with in vitro electrochemical oxidation of H2O2, ONOO-, NO*, and NO2(-) solutions. These results enabled the calculation of time variations of emission flux for each species and the reconstruction of the original flux of production of primary species, O2*- and NO*, by the macrophage.     

The fluorimetric FAST method, a simple tool for the optimization of microwave pasteurization of milk

The effect of microwave pasteurization of cow's milk on its nutritional quality was examined by the FAST method (Fluorescence of Advanced Maillard products and Soluble Tryptophan). Raw milk samples were submitted to different laboratory scale in batch microwave treatments using a central composite experimental design based on specific power and treatment time. The FAST index and bacterial count were monitored to assess protein denaturation, modification by the Maillard reaction and pasteurization efficiency, respectively. High discrimination between samples indicated that the FAST method is a potent tool for estimating the deterioration of the milk quality during experimental microwave treatment. Thus, the FAST index can be effectively used as the continuous response in experimental designs set up and to maximize information economically. In short, the FAST method allows us to retain the rapidity of experimental design while providing the advantages of convenience and low cost.     

Estimating the demand for public open space: Evidence from North Carolina municipalities*

This paper empirically identifies socio‐economic, physical, and geographic factors of the demand for open space in the state of North Carolina in the United States. Estimated coefficients suggest that spatial dependency exists in open space demand and that open space is a normal good. The paper provides the first empirical investigation of how open space demand is affected by local weather conditions. It is found that the demand has a statistically significant substitution relationship with nice weather. We discuss the socio‐economic rationale for the estimated demand models within the regional context of North Carolina and point to some relevant policy implications. Resumen. Este artículo identifica empíricamente los factores socioeconómicos, físicos y geográficos de la demanda de espacios al aire libre en el estado de Carolina del Norte en los Estados Unidos. Los coeficientes estimados sugieren que existe una dependencia espacial en la demanda de espacios al aire libre y que los espacios al aire libre son un bien normal. El artículo ofrece la primera investigación empírica sobre como se ve afectada la demanda de espacios al aire libre por las condiciones meteorológicas locales. Se ha encontrado que la demanda tiene una relación de substitución estadísticamente significativa con una meteorología benigna. Discutimos el fundamento socioeconómico de los modelos de demanda estimados dentro del contexto regional de Carolina del Norte e indicamos varias implicaciones relevantes para la formulación de políticas.      

Prevention of volatile fatty acids production and limitation of odours from winery wastewaters by denitrification

The effect of the addition of nitrate to winery wastewaters to control the formation of VFA in order to prevent odours during storage and treatment was studied in batch bioreactors at different NO(3)/chemical oxygen demand (COD) ratios and at full scale in natural evaporation ponds (2 x 7000 m(2)) by measuring olfactory intensity. In the absence of nitrate, butyric acid (2304 mgL(-1)), acetic acid (1633 mgL(-1)), propionic acid (1558 mgL(-1)), caproic acid (499 mgL(-1)) and valeric acid (298 mgL(-1)) were produced from reconstituted winery wastewater. For a ratio of NO(3)/COD=0.4 gg(-1), caproic and valeric acids were not formed. The production of butyric and propionic acids was reduced by 93.3% and 72.5%, respectively, at a ratio of NO(3)/COD=0.8, and by 97.4% and 100% at a ratio of NO(3)/COD=1.2 gg(-1). Nitrate delayed and decreased butyric acid formation in relation to the oxidoreduction potential. Studies in ponds showed that the addition of concentrated calcium nitrate (NITCAL) to winery wastewaters (3526 m(3)) in a ratio of NO(3)/COD=0.8 inhibited VFA production, with COD elimination (94%) and total nitrate degradation, and no final nitrite accumulation. On the contrary, in ponds not treated with nitrate, malodorous VFA (from propionic to heptano?c acids) represented up to 60% of the COD. Olfactory intensity measurements in relation to the butanol scale of VFA solutions and the ponds revealed the pervasive role of VFA in the odour of the untreated pond as well as the clear decrease in the intensity and not unpleasant odour of the winery wastewater pond enriched in nitrates. The results obtained at full scale underscored the feasibility and safety of the calcium nitrate treatment as opposed to concentrated nitric acid.     

Influence of a stormwater sediment deposit on microbial and biogeochemical processes in infiltration porous media

The purpose of this study was to test the relative influence of organic matter quantity and quality and the pollutant content of a stormwater sediment deposit on mineralization processes, microbial characteristics, and the release of solutes in infiltration sediment systems. In microcosm experiments, two other natural sediment deposits (one low and one rich particulate organic matter deposits) were studied to compare their effects with those of the stormwater deposit. The results showed that the biogeochemical processes (aerobic respiration, denitrification, fermentative processes), the microbial metabolism (enzymatic activities), and the releases of several solutes (NH(4)(+) and DOC) were stimulated in presence of the stormwater deposit and the natural particulate organic matter (POM)-rich deposit because of the quantity of the POM in these deposits. In the stormwater deposit, the high availability of the POM (indicated by its low C/N ratio and its high P content) produced a higher stimulation of the microbial metabolism than in presence of the POM-rich deposit (with a high C/N ratio). Pollutant (hydrocarbon and heavy metal) contents of the stormwater deposit did not have a significant effect on microbial processes. Thus, main effects of the stormwater sedimentary deposit on infiltration system were due to its organic matter characteristics (quantity and quality). Such organic matter characteristics need to be considered in future studies to determine the contamination potential of stormwater management practices.     

Aerobic Biodegradation of Acetylated Starch

Some acetylated starch samples with degrees of substitution varying from 0.03 to 1.6 are submitted to aerobic degradation by Bacillus amyloliquefaciens. The degradation is expressed by the concentration of reducing sugars evolved from the substrate, by the amylolytic activity and by the assessment of the CO₂ release resulting from the mineralization of the acetylated starch. With formation of exclusively glucose and maltose as control substrates, the hydrolysis is complete. For the samples with a low acetylation degree, the hydrolysis is slowed down as a result of inhibition by the acetyl groups. When the degree of acetylation is high, it becomes necessary to add inducers of acetylesterase activity. So, for acetylated starch with a degree of substitution of 1.6, the mineralization percentage increases up to 30% upon addition of isopropyl-β-D -thiogalactopyranoside.