Investigation of the biopharmaceutical behavior of theophylline hydrophilic matrix tablets using USP methods and an artificial digestive system

This work aimed to investigate the biopharmaceutical behavior of hydrophilic matrix tablets of theophylline using different in vitro methods: USP II, USP IV, and a novel in vitro system simulating the gastrointestinal tract in man called the artificial digestive system (ADS). The potentiality of each method was evaluated by establishing in vitro/in vivo correlation. Using USP methods, the drug release was pH-independent and dependent on agitation intensity. Level A IVIVCs could be established using the different in vitro methods but one to one correlation was established only when the ADS method was used. For the prediction of in vivo drug dosage form behavior based on in vitro methods, the ADS showed a high predictability when compared to USP in vitro methods.     

Photopatterning of polybutadiene substrates by interferometric ultraviolet lithography: fabrication of phospholipid microarrays

Gratings are written holographically with low power (10 mW/cm(2)) 244 nm UV light on thin films of polybutadiene rubber polymer. The increase of hydrophilicity-wettability of polybutadiene films is measured over UV-exposed regions. Sequential fabrication of two orthogonal gratings results in hydrophilic microarrays having applications as functionalized substrates for immobilizing biomolecules. This is demonstrated by immobilizing a phospholipid in a microarray pattern.     

Authenticating white grape must variety with classification models based on aroma sensors, FT-IR and UV spectrometry

This paper aims at assessing the capability of high-speed analytical devices, such as aroma sensors (“electronic noses”), Fourier Transform InfraRed (FT-IR) and ultraviolet spectrometers to classify white grape musts (grape juices before fermentation) in variety categories. Due to the complexity of the signal generated, specific data processing techniques have been developed and are described here. First, a pre-processing technique, based on Genetic Algorithms, is applied to spectra to improve spectrometer efficiency without expert knowledge in spectrometry; by selecting the most discriminant subsets of wavelengths, this stochastic method tends to reduce over-fitting and improves classification results. Secondly, the Partial Least Squares Regression technique is adapted to a pattern recognition problem, using Partial Least Squares-Discriminant Analysis, a multivariate classification technique. These devices and data processing techniques are applied to more than 100 must samples. FT-IR spectrometry is the most satisfactory technique with a 9.6% classification error level. Finally, outputs of the three individual sensors are combined in a “low-level” fusion method, by concatenating the individual sensor signals. This straightforward fusion method does not significantly improve results.     

Structural Characterization of Highly Conducting AlGaN (<Emphasis Type="Italic">x</Emphasis> > 50%) for Deep-Ultraviolet Light-Emitting Diode

Off-axis reciprocal-space mapping was performed on aluminum gallium nitride deep-ultraviolet light-emitting diode base layers. The results indicate that aluminum gallium nitride films growing on aluminum nitride-on-sapphire templates initially grow in compression, nearly lattice matched to the relaxed aluminum nitride buffer layer with approximately 0.5% biaxial strain. This compressive strain may be partially relieved over the course of the thick aluminum gallium nitride growth when a high-quality aluminum nitride and superlattice layer is used. Additionally, a growth interruption appears to allow growth of an unstrained aluminum gallium nitride layer without a gradual release of compressive strain. Growth on a bulk aluminum nitride substrate appears to yield an aluminum gallium nitride layer in tension rather than compression.     

Ductile tearing predictions with Wellman’s failure model

Predictions for the Sandia National Laboratories fracture challenge (Boyce et al. in Int J Fract 2013) were generated using a transient dynamic finite element code with a multi-linear elastic plastic failure model developed by Wellman (Simple approach to modeling ductile failure. Sandia National Laboratories, Albuquerque 2012). This model is a conventional, rate independent, von Mises plasticity model for metals with user-prescribed hardening as a function of equivalent plastic strain. In addition to conventional plasticity, this model has empirical criteria for crack initiation and growth. Ductile tearing predictions generated with this model were found to be in good agreement with experimental measurements and observations.     

Influence of manufacturing conditions on the conjugated linoleic acid content and the isomer composition in ripened French Emmental cheese

In a study of the evolution of conjugated linoleic acid (CLA) during cheese production, the influence of Emmental cheese processing on the CLA content and the CLA isomer composition was evaluated. The use of raw and thermised milk, changes of processing temperature and the effect of propionic acid bacteria (PAB) were investigated. The content of CLA in raw milk was 8.6 +/- 1.9 mg/g fat and in the ripened cheese at 70 d was 8.6 +/- 1.6 mg/g fat, under normal processing conditions. No changes in the CLA content and CLA isomer composition were observed during Emmental cheese manufacturing process. Changes in cooking and moulding temperatures did not influence the CLA content. CLA content of cheese made from microfiltered milk with two different Propionibacterium freudenreichii strains was very close to cheeses made without PAB. CLA levels seem to be stable in this type of dairy product under the conditions examined.     

Roles of structural phenylpropanoids in forage cell wall digestion

Phenolic constituents (lignins and phenolic acids) and carbohydrates are assembled in a tight architecture which differs according to the plant species. During cell wall digestion, the hydrolysis kinetics differ between carbohydrates and seem to depend chiefly on the content and organisation of tissue phenolics. Among the phenylpropanoids, ferulic acid is released more quickly than p-coumaric acid. Lignins remain largely in the cell walls. They also undergo transformations, chiefly solubilisation as lignin-carbohydrate complexes. The limiting effect of lignins on cell wall degradation increases with increasing content. However, their effect on degradation might also depend on qualitative factors such as lignin structure and polymer organisation in walls and tissues. When various grasses (normal and selected genotypes), or grasses and legumes are compared, correlations between certain factors such as lignin uncondensed fraction, syringyl units or phenolic acids contents and cell wall degradation emerge but not clear causal relationship has been shown. Nonetheless, other structural characteristics, related to the alkali reactivity of lignins, seem to have a stronger influence on cell wall degradation. Phenylpropanoids seem to act mainly as a physical and (bio)chemical barrier to the action of the microbial enzymes. In addition, their reactivity as phenolic compounds and their hydrophobicity seem to play a role. Digestion is not limited only by phenolics. The factors that limit glycanolysis—the accessibility, crystallinity and capillary structure of cellulose and the branching of hemicelluloses—seem to have little or no effect on cell wall degradation in vivo. In contrast, other antiquality substances (tannins, cutin and silica), plant antomy, environmental factors, factors modulating microbial growth and animal physiology influence cell wall utilisation. Future research in this field should focus on the effects of phenolic structure and of cell wall and tissue organisation on carbohydrate degradation.     

A case of behavioral heterosis in mice: Quantitative and qualitative aspects of performance in a water-escape task.

1,404 mice from 2 inbred strains (BALB/c and C57BL/6), F?, F?, and backcrosses were subjected to 4 trials in a water-escape task and a swimming test. Detailed analysis of Ss' behavior in these situations showed that the "F? hybrid vigor" affected behavioral characters not directly related to physical vigor but of potential adaptive value. Their superiority was mainly due to more frequent adoption of an efficient behavioral tactic (direct or edge escape paths toward the exit) and more rapid progress with experience in this respect than other generations exhibited. Results show that heterosis is not limited to physical vigor but may extend to behavioral and even psychological characters. (19 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)