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131.
132.
Projection of transmembrane helices using a Uniform B-spline Algorithm is a tool for the visualization of interactions between helices in membrane proteins. It allows the user to generate projections of 3D helices, no matter what their deviations from a canonical helix might be. When associated with adapted coloring schemes it facilitates the comprehension of helix-helix interactions. Examples of transmembrane proteins were chosen to illustrate the advantages that this method provides. In the glycophorin A dimer we can easily appreciate the structural features behind homodimerisation. Using the structure of the fumarate reductase we analyze the contact surfaces inside a helical bundle and thanks to structures from a molecular dynamics simulation we see how modifications in structure and electrostatics relate to their interaction. We propose the use of this tool as an aid to the visualization and analysis of transmembrane helix surfaces and properties. 相似文献
133.
Jean-Michel Lucas Philippe Berticat Bernard Chabert Jean-Pierre Soulier Louis Gavet Noëlle Valentin 《大分子材料与工程》1977,65(1):103-120
Sizing is generally employed to protect yarns when submitted to mechanical stress during weaving. Two commercial acrylic copolymers used for sizing of polyester yarns are studied: an acrylonitril-acrylic acid copolymer (A) as a bad sizing product and an acrylonitril-acrylic acid-ethyl acrylate copolymer (B) as a good one. A relationship has been established between physico-chemical parameters and sizing efficiency. Interactions between polyester yarn and size (and samples used as models) are studied both in solution and in dry state. It must be pointed out that the action of water is basically due to its plasticizing properties. The presence of ethyl acrylate favorizes the polyester yarn impregnation with size B in aqueous solution. Adhesion in the dry state is mainly caused by acrylic acid. Finally the action of neutralizing agents and imbeding oils is discussed. 相似文献
134.
Laurencia T. Songré-Ouattara Claire Mouquet-Rivier Christèle Humblot Isabelle Rochette Bréhima Diawara Jean-Pierre Guyot 《Journal of food science》2010,75(5):M261-M269
Abstract: To assess the ability of lactic acid bacteria to improve some nutritional characteristics of the pearl millet–soybean slurry to prepare complementary foods for young children in African countries, inoculation was performed using strains previously selected for their ability to hydrolyse starch, phytate, or α-galactooligosaccharides (α-GOS). For the sake of comparison with the action of a natural microflora, fermentation was also performed by back slopping inoculation, that is, with a sample obtained from spontaneously fermented traditional pearl millet slurry obtained from a small scale processing unit in Burkina Faso (Ouagadougou). Starter cultures thrived on the slurry as shown by counts on MRS agar, TTGE fingerprints, and fermentation patterns. The fermentation of precooked slurries inoculated by back slopping or with mixed cultures containing the amylolytic strain Lb. plantarum A6 enabled partial starch hydrolysis. Corresponding gruels had a suitable consistency for young child feeding at high dry matter content, and a high energy density: 88.7 ± 4.2 and 75.8 ± 5.1 kcal/100 g of sweetened gruel, for the gruels inoculated by back slopping or with Lb. plantarum A6, respectively. Unexpectedly, no decrease in phytates was observed in any of the experiments, suggesting the presence of one or more inhibitory compounds in soybean. Furthermore, preprocessing conditions before fermentation affect the carbohydrate composition of slurry and have a more profound effect than fermentation on the reduction of the α-GOS content. Practical Application: This research aims to investigate the capacity of selected lactic acid bacteria to produce in a bioprocess high energy density and nutritious gruels for complementary feeding of young children from an African cereal (pearl millet) mixed with soybean. 相似文献
135.
Msayer is a dry salted lemon used as an aromatising condiment in many North-African food recipes. This preparation is obtained by adding coarse salt (10 g/100 g fresh lemons) to fresh cut lemons in containers kept under dark conditions away from air for around a month and a half. A survey of 52 days showed low initial uptake of citric acid and sugars from the formed liquid phase. Nevertheless, there were still sugars detected even at the end of the survey attesting for the low microbial activity as also shown by carbon dioxide production and microbial viable counts. Indeed, the microbial counts showed a steady decrease reaching 2 log units at the 52nd day of curing. Moulds were detected only at the beginning but at low numbers. No viable counts of lactic acid bacteria were observed on MRS medium despite detection of lactic acid (8 g/l). In contrast, yeasts seem to dominate in the msayer preparation. They count for almost all the microbial population especially after the first week. Six of these acid and halotolerant yeast isolates were identified, by sequence analysis of the amplified 5.8S rRNA and the two internal transcribed spacer regions, as Candida parapsislosis (2 isolates) and unclassified Saccharomycetales (4 isolates). 相似文献
136.
Bahi Manoubi Auguste Fremont Hlne Landesman Jean-Pierre Gentil Annabelle Lecuyer Pascal 《Microelectronics Reliability》2009,49(9-11):1273-1277
We describe a new methodology for the “in situ” identification of wire-bond degradation at early stages during high-temperature aging tests on devices with standard plastic packages. This methodology is based on the measurement of the changes in wire bond resistance, which is deduced from the I(V) characteristics of the ESD protection diodes on each contact pad of the circuit. In a first stage, the measurement procedure is described, with emphasis on the initial temperature calibration. This procedure allows for an “in situ” measurement sequence, where the packages stay in the aging chamber, at elevated temperature, during the electrical tests on the pad connections performed at different aging durations. By following accurately the package temperature, using a thermocouple, it is possible to correct for slight changes and thus get a reliable I–V measurement for each interconnection. In the second stage, the aging test results are described, showing the evolution of each individual interconnection. We were able to identify the onset of wire-bond degradation through the progressive increase of their resistance. To allow for better determination of the degradation process, once an increase in wire bond resistance was detected, complete I(V) curves were recorded at the pin(s) of interest. For each pin of a TQFP64 package, the tests were performed at least twice a day, with increased density when initial failure is detected (one complete measurement every 3 h). This strategy allowed for the detection of different behaviors on the wire bonds: good ball bonds (i.e. ball bonds with no change in their resistance), ball bond with intermittent opens (these ball bonds are in the process of degradation, and thermo-mechanical stresses induced in the resin by very small temperature changes are sufficient to open or close the circuits) and completely destroyed ball bonds, for which the resistance stays in an “high” level. This approach to wire-bond degradation in plastic packages is very powerful in terms of the number of interconnections which can be followed “in real time” and especially has the advantage, over other classical approaches, that the devices under test stay operational, contrary to what occurs with other types of destructive testing. These electrical test results are compared with metallographic investigations performed after a series of mechanical tests on the ball bonds (wire pull/ball shear tests) on a set of identical devices which undergone exactly the same High Temperature Storage (HTS) aging for 2000 h at 165 °C. 相似文献
137.
Chi-Woo Lee Dimitri Lederer Aryan Afzalian Ran Yan Nima Dehdashti Akhavan Jean-Pierre Colinge 《Microelectronic Engineering》2009,86(10):2067-2071
As MuGFETs are promising contenders for the end of the silicon Roadmap, their high-temperature behaviour needs to be addressed. In this work we investigate the variations of the subthreshold slope (SS) of double-gate devices and MuGFETs with intrinsic doping as a function of the temperature and fin width. Focus is placed on the superlinear behaviour of SS occurring above a certain temperature threshold. Numerical simulations are performed using Comsol Multiphysics™ and a 1D analytical model is developed. The model, which includes the effect of film and gate oxide thickness, is shown to accurately fit the numerical data. A new definition for the subthreshold slope under high-temperature operation is proposed. The high-temperature subthreshold slope degradation is shown to increase with fin width. 相似文献
138.
Caroline Celle Clément Suspène Jean-Pierre Simonato Stéphane Lenfant Marc Ternisien Dominique Vuillaume 《Organic Electronics》2009,10(1):119-126
In this paper we show that thiolated self-assembled monolayers (SAMs) can be used to anchor source–drain gold electrodes on the substrate, leading to excellent electrical performances of the organic field-effect transistor (OFET) on a par with those using a standard electrode process. Using an amorphous semiconductor and a gate dielectric functionalized with SAMs bearing different dipole moments, we demonstrate that we can tune the threshold voltage alone, while keeping nearly unchanged the other electrical properties (hole carrier mobility, Ion/Ioff ratio, subthreshold swing). This differs from previous studies for which SAMs functionalization induced significant changes in all the OFET electrical performances. This result opens doors to design organic circuits using reproducible amorphous semiconductor based OFETs for which only the threshold voltage can be tuned on demand. 相似文献
139.
Jose Manuel López-Cepero Sheldon M. Wiederhorn David Black Jean-Pierre Guin Antonio R. de Arellano-López Julian Martínez-Fernández 《Journal of the American Ceramic Society》2009,92(4):845-849
Single-crystal sapphire exhibits a highly anisotropic fracture behavior. The surfaces of specimens fractured along prismatic planes are wavy, with fractographic features appearing as small areas of contrast under an optical microscope. Optical microscopy, atomic force microscopy, X-ray topography, and confocal microscopy are used to demonstrate a correlation between the areas of contrast and dislocations penetrating the sample surface. The surface features are argued to be a consequence of the stress field surrounding the dislocations, which deflect the crack approximately 10 nm normal to the surface as the crack cuts the dislocations. The lateral extent of measurable surface deflection is of the order of 5 μm. These images can be compared directly to show the equivalence of the position of the dislocations observed by X-ray topography and the surface contrast observed optically. 相似文献
140.