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11.
Johnson J 《Environmental science & technology》1996,30(11):481A-482A
Society. 相似文献
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Calmodulin-dependent protein kinase II (Cam kinase II) is known to desensitise epidermal growth factor receptor (HER-1) tyrosine kinase activity by a process involving phosphorylation at serines 1046/47 in the cytoplasmic tail. We have developed an experimental system to investigate phosphorylation of the related HER-2/c-erbB2 proto-oncogene utilising purified Cam kinase II and recombinant glutathione-S-transferase (GST) fusion proteins. The cDNA for rat Cam kinase II-alpha was transfected into human embryonic kidney (HEK) 293 fibroblasts and the expressed protein purified to homogeneity by calmodulin-agarose affinity chromatography. A GST fusion protein comprising residues 1126-1255 of HER-2 was phosphorylated by purified Cam kinase II, in contrast to a GST protein comprising residues 1005-1125. Phosphoamino-acid analysis and site-directed mutagenesis indicated that HER-2 was phosphorylated on a single site at threonine-1172 which resides within a consensus Cam kinase II phosphorylation site (RAKT). HER-2 (threonine-1172-alanine), in the form of a ligand-inducible chimaera HER-1/2, was co-transfected into HEK-293 fibroblasts with a constitutively active form of Cam kinase II, followed by in vivo labelling of these cells with 32 P-orthophosphate. Immunoprecipitation of ligand-activated receptors followed by two-dimensional phosphopeptide mapping indicated that threonine-1172 in HER-2 is a newly identified in vivo site which can be hyper-phosphorylated by constitutively active Cam kinase II. In addition, when over-expressed in HEK-293 fibroblasts, HER-1/2 (threonine-1172-alanine) showed a defect in desensitisation and underwent a more sustained EGF-induced receptor autophosphorylation compared to wild-type HER-1/2. 相似文献
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A microbubble-powered bioparticle actuator 总被引:4,自引:0,他引:4
Maxwell R.B. Gerhardt A.L. Toner M. Gray M.L. Schmidt M.A. 《Journal of microelectromechanical systems》2003,12(5):630-640
We present the results of a device that uses controllable microbubble actuation to manipulate bioparticles. In order to create a useful device for controlling the position of bioparticles, predictable microfluidic actuation is crucial. The goal of this project was to develop fundamental technology that can be used to manipulate single bioparticles (e.g., cells). We use a thermal bubble actuation method to accomplish this goal. Microbubbles have the advantages of relatively simple electronics and fabrication but can be difficult to control. In this paper, we describe two specific accomplishments: the use of micromachined nucleation cavities to precisely localize thermal bubbles and to achieve controllable bubble formation temperatures and bubble dissipation and the demonstration of controllable microbubbles in a new device for particle sorting. 相似文献
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Jeff Munch 《通讯世界》2003,9(6):102
~~CompactPCI系统管理@Jeff Munch$凌华科技!技术长暨国际PICMG 3.0委员会主席 相似文献
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Xing Gu Shariar Sabuktagin Ali Teke Daniel Johnstone Hadis Morkoç Bill Nemeth Jeff Nause 《Journal of Materials Science: Materials in Electronics》2004,15(6):373-378
ZnO is a highly efficient photon emitter, and has optical and piezoelectric properties that are attractive for a variety of applications in sensors and potentially optoelectronic devices such as emitters. Due to its identical stacking order and close lattice match to GaN, it is also being developed as a substrate material for GaN epitaxy. However, the surface finish of the ZnO is such that much of the damage induced by sawing and follow up mechanical polishing remains. We developed a thermal treatment method to eliminate surface damage on the 0 face of ZnO (0 0 0 1) to prepare it for epitaxial growth. Atomic force microscopy images of ZnO (0 0 0 1) annealed at 1050 °C for 3 h etc. show that residual scratches from mechanical polishing are removed and atomically flat, terrace-like surfaces are attained. In addition, low-temperature photoluminescence and high-resolution X-ray diffraction measurements have been employed to investigate the effect of annealing on ZnO substrates. 相似文献
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基于电子设备的处理器系统设计人员经常在选择最佳电源架构时遇到困难。有时最佳的解决方案是插入式电源,而有时采用分立的元件组成的电源才是最佳解决方案。选择插入式电源解决方案相对来说比较直接,但对于缺乏电源设计经验的数字设计人员来说,设计一个分立电源解决方案可能会使他望而却步。大多 DC/DC 电源控制 IC 供应商均可提供有助于电路设计的详细辅助材料。但是,在开始电源设计之前,设计人员必须选择正确的拓扑。本文将介绍以下方法,来帮助为某些用于MCU、DSP及基于FPGA 的电子产品的最常用结构选择正确的电源拓扑。线性调节… 相似文献
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