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71.
Guldenmund F Hale A Goossens L Betten J Duijm NJ 《Journal of hazardous materials》2006,130(3):575-241
This paper describes the development of a management model to control barriers devised to prevent major hazard scenarios. Additionally, an audit technique is explained that assesses the quality of such a management system. The final purpose of the audit technique is to quantify those aspects of the management system that have a direct impact on the reliability and effectiveness of the barriers and, hence, the probability of the scenarios involved.
First, an outline of the management model is given and its elements are explained. Then, the development of the audit technique is described. Because the audit technique uses actual major hazard scenarios and barriers within these as its focus, the technique achieves a concreteness and clarity that many other techniques often lack. However, this strength is also its limitation, since the full safety management system is not covered with the technique. Finally, some preliminary experiences obtained from several test sites are compiled and discussed. 相似文献
72.
基于先进科技对准规格的减缩,对准仪量测数据的应用也变得更为重要。不同对准策略的选择所引起的结果,对全部对准预算有很重要的影响。介绍两个主要科技研发项目:先进对准配套和对准标的设计。 相似文献
73.
Validation of the performance of a GMO multiplex screening assay based on microarray detection 总被引:1,自引:1,他引:0
Serge Leimanis Sandrine Hamels Florence Nazé Guillaume Mbongolo Mbella Myriam Sneyers Rupert Hochegger Hermann Broll Lillian Roth Klára Dallmann Adrienn Micsinai José Luis La Paz Maria Pla Claudia Brünen-Nieweler Nina Papazova Isabel Taverniers Norbert Hess Britta Kirschneit Yves Bertheau Colette Audeon Valérie Laval Ulrich Busch Sven Pecoraro Katrin Neumann Sibylle Rösel Jeroen van Dijk Esther Kok Gianni Bellocchi Nicoletta Foti Marco Mazzara William Moens José Remacle Guy Van Den Eede 《European Food Research and Technology》2008,227(6):1621-1632
A new screening method for the detection and identification of GMO, based on the use of multiplex PCR followed by microarray, has been developed and is presented. The technology is based on the identification of quite ubiquitous GMO genetic target elements first amplified by PCR, followed by direct hybridisation of the amplicons on a predefined microarray (DualChip® GMO, Eppendorf, Germany). The validation was performed within the framework of a European project (Co-Extra, contract no 007158) and in collaboration with 12 laboratories specialised in GMO detection. The present study reports the strategy and the results of an ISO complying validation of the method carried out through an inter-laboratory study. Sets of blind samples were provided consisting of DNA reference materials covering all the elements detectable by specific probes present on the array. The GMO concentrations varied from 1% down to 0.045%. In addition, a mixture of two GMO events (0.1% RRS diluted in 100% TOPAS19/2) was incorporated in the study to test the robustness of the assay in extreme conditions. Data were processed according to ISO 5725 standard. The method was evaluated with predefined performance criteria with respect to the EC CRL method acceptance criteria. The overall method performance met the acceptance criteria; in particular, the results showed that the method is suitable for the detection of the different target elements at 0.1% concentration of GMO with a 95% accuracy rate. This collaborative trial showed that the method can be considered as fit for the purpose of screening with respect to its intra- and inter-laboratory accuracy. The results demonstrated the validity of combining multiplex PCR with array detection as provided by the DualChip® GMO (Eppendorf, Germany) for the screening of GMO. The results showed that the technology is robust, practical and suitable as a screening tool. 相似文献
74.
Lai J Ru G Ingravalle F Choiseul J Jørgensen JS Millan MB Vancutsem J Wernitznig F Marchis D Decastelli L 《Journal of food protection》2008,71(3):578-583
The bovine spongiform encephalopathy epidemic is thought to have occurred as a consequence of feeding prion-infected material to cattle. To avoid the risk of bovine spongiform encephalopathy diffusion, the European Commission (Directive 2003/126/EC) established an official method to detect the presence of animal-derived constituents in feedstuffs, using microscopic examination. This method allows easy identification of bone fragments among other animal constituents. The analysis is based on morphological conformation of the fragments and their characterization (mainly of the shape of lacunae) to discriminate among mammalian, poultry, and fish tissues. The aim of this study was to assess the performances of nine European laboratories through a ring trial of the official microscopic method, and to calculate accuracy and reproducibility of the method. In general the reproducibility of the microscopic method was very good (kappa overall = 0.83), with a high sensitivity for all laboratories. Concerning the analysis on the different animal-derived constituents, the results show values of sensitivity with large variability between fish and poultry or mammal. It was generally more difficult to discriminate between mammalian and poultry tissues than fish tissue. 相似文献
75.
Antimicrobial resistance profiles of 5 common bovine mastitis pathogens in large Chinese dairy herds
Jia Cheng Weijie Qu Herman W. Barkema Diego B. Nobrega Jian Gao Gang Liu Jeroen De Buck John P. Kastelic Hong Sun Bo Han 《Journal of dairy science》2019,102(3):2416-2426
The prevalence of antimicrobial resistance (AMR) is increasing in human and animal pathogens, becoming a concern worldwide. However, prevalence and characteristics of AMR of bovine mastitis pathogens in large Chinese dairy herds are still unclear. Therefore, our objective was to determine the AMR profile of bacteria isolated from clinical mastitis in large (>500 cows) Chinese dairy herds. A total of 541 isolates of the 5 most common species, Staphylococcus aureus (n = 103), non-aureus staphylococci (NAS; n = 107), Streptococcus species (n = 101), Klebsiella species (n = 130), and Escherichia coli (n = 100), isolated from bovine clinical mastitis on 45 dairy farms located in 10 provinces of China were included. Presence of AMR was determined by minimum inhibitory concentrations using the microdilution method. Prevalence of multidrug resistance (resistance to >2 antimicrobials) was 27% (148/541). A very wide distribution of minimum inhibitory concentrations was screened in all isolates, including Staph. aureus isolates, which were resistant to penicillin (66%). In addition, NAS (30%) were more resistant than Staph. aureus to oxacillin (84%), penicillin (62%), tetracycline (34%), and clindamycin (33%). Prevalence of resistance to tetracycline was high (59%) in Streptococcus spp. Additionally, prevalence of resistance of both E. coli and Klebsiella spp. was high to amoxicillin/clavulanate potassium (81 and 38%, respectively), followed by tetracycline (only Klebsiella spp. 32%). A high proportion (27%) of isolates were multidrug resistant; the most frequent combinations were clindamycin-cefalexin-tetracycline or enrofloxacin-cefalexin-penicillin patterns for Staph. aureus; enrofloxacin-oxacillin-penicillin-tetracycline patterns for NAS; clindamycin-enrofloxacin-tetracycline patterns for Streptococcus spp.; amoxicillin/clavulanate potassium-ceftiofur-polymyxin B patterns for Klebsiella spp.; and amoxicillin/clavulanate potassium-ceftiofur-polymyxin B patterns for E. coli. Resistance for 4 kinds of antimicrobials highly critical for human medicine, including daptomycin, vancomycin, imipenem, and polymyxin B, ranged from 0 to 24%. In conclusion, prevalence of AMR in mastitis pathogens was high on large Chinese dairy farms, potentially jeopardizing both antimicrobial efficacy and public health. Results of this study highlighted the need for improvements in antimicrobial stewardship and infection control programs in large Chinese dairy farms to reduce emergence of AMR. 相似文献
76.
Innovations in Systems and Software Engineering - In black-box checking (BBC) incremental hypotheses on the behavior of a system are learned in the form of finite automata, using information from a... 相似文献
77.
Jeroen van Ameijde Chun Yu Ma Garvin Goepel Clive Kirsten Jeff Wong 《建筑学研究前沿(英文版)》2022,11(2):308-323
In the context of ongoing densification of cities and aging urban populations, public spaces are a crucial infrastructure to support the physical and mental wellbeing of urban residents. The design of public space furniture elements is often standardised, and not considered in relation to environmental conditions and mechanisms of social interaction. This article presents a digital workflow to generate site-specific designs for shaded public seating, considering the relationships of local public places to their surroundings. A strategy for customised and site-specific design is developed through the use of multiple software tools, employing evolutionary algorithms and multi-objective optimisation. The method is applied to a small public space canopy prototype installed within a public housing estate in Hong Kong, incorporating additional criteria to achieve a low-cost and light-weight structure. Through multiple stages of refinement and optimisation, a material, structural and social performance-driven outcome was achieved that creates a shaded space for public seating, people watching and social interaction. As part of a larger research agenda exploring architectural form-finding and environmental psychology, the project represents potential new applications in the emerging field of socially driven computational design. 相似文献
78.
79.
Jan Bekkering Kor Zwart Gerard Martinus Jort Langerak Jeroen Tideman Tineke van der Meij Kees Alberts Machiel van Steenis Jan-Peter Nap 《国际能源研究杂志》2020,44(3):2264-2277
Power-to-gas technologies are considered to be part of the future energy system, but their viability and applicability need to be assessed. Therefore, models for the viability of farm-scale bio-power-to-methane supply chains to produce green gas were analysed in terms of levelised cost of energy, energy efficiency and saving of greenhouse gas emission. In bio-power-to-methane, hydrogen from electrolysis driven by surplus renewable electricity and carbon dioxide from biogas are converted to methane by microbes in an ex situ trickle-bed reactor. Such bio-methanation could replace the current upgrading of biogas to green gas with membrane technology. Four scenarios were compared: a reference scenario without bio-methanation (A), bio-methanation (B), bio-methanation combined with membrane upgrading (C) and the latter with use of renewable energy only (all-green; D). The reference scenario (A) has the lowest costs for green gas production, but the bio-methanation scenarios (B-D) have higher energy efficiencies and environmental benefits. The higher costs of the bio-methanation scenarios are largely due to electrolysis, whereas the environmental benefits are due to the use of renewable electricity. Only the all-green scenario (D) meets the 2026 EU goal of 80% reduction of greenhouse gas emissions, but it would require a CO2 price of 200 € t−1 to achieve the levelised cost of energy of 65 €ct Nm−3 of the reference scenario. Inclusion of the intermittency of renewable energy in the scenarios substantially increases the costs. Further greening of the bio-methanation supply chain and how intermittency is best taken into account need further investigation. 相似文献
80.
Jan Rinkel Prof. Dr. Jeroen S. Dickschat 《Chembiochem : a European journal of chemical biology》2020,21(6):807-810
Isotopic labeling experiments performed with a newly identified bacterial trichoacorenol synthase established a 1,5-hydride shift occurring in the cyclization mechanism. During EI-MS analysis, major fragments of the sesquiterpenoid were shown to arise via cryptic hydrogen movements. Therefore, the interpretation of earlier results regarding the cyclization mechanism obtained by feeding experiments in Trichoderma is revised. 相似文献