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排序方式: 共有602条查询结果,搜索用时 78 毫秒
91.
Buters JT Schober W Gutermuth J Jakob T Aguilar-Pimentel A Huss-Marp J Traidl-Hoffmann C Mair S Mair S Mayer F Breuer K Behrendt H 《Environmental science & technology》2007,41(7):2622-2629
The interior of motor vehicles is made of a wide variety of synthetic materials, which emit volatile organic compounds (VOC). We tested the health effects of emissions from vehicles exposed to "parked in sunshine" conditions. A new and a 3 year old vehicle with identical interior were exposed to 14 000 W of light. Indoor air was analyzed by GC-MS. Toxicity of extracts of indoor air was assayed in human primary keratinocytes, human lung epithelial A549 cell line, and Chinese hamster V79 lung fibroblasts. In addition, toxicity after metabolic activation by CYP1A1, CYP1A2, CYP1B1, CYP2A6, CYP2B6, and CYP2E1 was assayed. The effect on type I allergic reaction (IgE-mediated immune response), type IV allergic reaction (T-cell mediated immune response), and irritative potential was evaluated also. A total of 10.9 and 1.2 mg/m(3) VOC were found in new and used motor vehicle indoor air, respectively. The major compounds in the new vehicle were o,m,p-xylenes, C3 and C4-alkylbenzenes, dodecane, tridecane, and methylpyrrolidinone. In the used vehicle they were acetone, methylpyrrolidinone, methylcyclohexane, acetaldehyde, o,m,p-xylenes, ethylhexanol, and toluene. No toxicity was observed in any cell line with or without metabolic activation. Neither did we find an effect on type IV sensitization or an irritative potential. A slight but statistically significant aggravating effect on IgE-mediated immune response of only the new vehicle indoor air was determined (p < 0.05). The IgE-response modulating effect of indoor air might be relevant for atopic individuals. Else no direct toxicity, no toxicity after metabolic activation by cytochrome P450, and no irritative or type IV sensitizing potential of motor vehicle indoor air were found, neither from the new nor used vehicle. Our investigations indicated no apparent health hazard of parked motor vehicle indoor air. 相似文献
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93.
Comellas-Aragonès M Engelkamp H Claessen VI Sommerdijk NA Rowan AE Christianen PC Maan JC Verduin BJ Cornelissen JJ Nolte RJ 《Nature nanotechnology》2007,2(10):635-639
Most enzyme studies are carried out in bulk aqueous solution, at the so-called ensemble level, but more recently studies have appeared in which enzyme activity is measured at the level of a single molecule, revealing previously unseen properties. To this end, enzymes have been chemically or physically anchored to a surface, which is often disadvantageous because it may lead to denaturation. In a natural environment, enzymes are present in a confined reaction space, which inspired us to develop a generic method to carry out single-enzyme experiments in the restricted spatial environment of a virus capsid. We report here the incorporation of individual horseradish peroxidase enzymes in the inner cavity of a virus, and describe single-molecule studies on their enzymatic behaviour. These show that the virus capsid is permeable for substrate and product and that this permeability can be altered by changing pH. 相似文献
94.
95.
Linqing Li Megan E. Griebel Marina Uroz Saniya Yesmin Bubli Keith A. Gagnon Britta Trappmann Brendon M. Baker Jeroen Eyckmans Christopher S. Chen 《Advanced functional materials》2024,34(17):2309567
Although tissue culture plastic has been widely employed for cell culture, the rigidity of plastic is not physiologic. Softer hydrogels used to culture cells have not been widely adopted in part because coupling chemistries are required to covalently capture extracellular matrix (ECM) proteins and support cell adhesion. To create an in vitro system with tunable stiffnesses that readily adsorbs ECM proteins for cell culture, a novel hydrophobic hydrogel system is presented via chemically converting hydroxyl residues on the dextran backbone to methacrylate groups, thereby transforming non-protein adhesive, hydrophilic dextran to highly protein adsorbent substrates. Increasing methacrylate functionality increases the hydrophobicity in the resulting hydrogels and enhances ECM protein adsorption without additional chemical reactions. These hydrophobic hydrogels permit facile and tunable modulation of substrate stiffness independent of hydrophobicity or ECM coatings. Using this approach, it is shown that substrate stiffness and ECM adsorption work together to affect cell morphology and proliferation, but the strengths of these effects vary in different cell types. Furthermore, it is revealed that stiffness-mediated differentiation of dermal fibroblasts into myofibroblasts is modulated by the substrate ECM. The material system demonstrates remarkable simplicity and flexibility to tune ECM coatings and substrate stiffness and study their effects on cell function. 相似文献
96.
Starch plastic sheets were prepared by compression molding of starch‐based plastic granulates. The granulates were prepared by extrusion processing of mixtures of granular potato starch and several maltodextrins (5% w/w) in the presence of glycerol and water as plasticizers and lecithin as melt flow accelerator. The materials were semicrystalline, containing B‐type, Vh‐type, and Eh‐type crystallinity. The properties were dependent on water content. For the materials, a brittle‐to‐ductile transition occurred at a water content in the range of 11–12%, which was in accordance with the observed glass transition temperature. The structural and mechanical properties were a function of starch composition and maltodextrin source as well as molding temperature. The amount of granular remnants and residual B‐type crystallinity decreased with increasing processing temperature. The amount of recrystallized single‐helical amylose increased with increasing temperature. At molding temperatures in the range of 180–200°C, a sharp decrease in starch molecular mass occurred. The influence of molding temperature was reflected in a sharp increase in elongation at molding temperature above 160°C and a gradual decrease in elastic modulus. The tensile strength showed an initial small increase up to 160°C and a sharp decrease at higher molding temperatures. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 74: 2207–2219, 1999 相似文献
97.
98.
Ingrid A.E. Spanjers Pieter WoutersTamara van Gog Jeroen J.G. van Merriënboer 《Computers in human behavior》2011,27(1):46-52
Many animations impose a high cognitive load due to the transience of information, which often hampers learning. Segmentation, that is presenting animations in pieces (i.e., segments), has been proposed as a means to reduce this high cognitive load. The expertise reversal effect shows, however, that design measures that have a positive effect on cognitive load and learning for students with lower levels of prior knowledge, might not be effective, or might even have a negative effect on cognitive load and learning for students with higher levels of prior knowledge. This experiment with animated worked-out examples showed an expertise reversal effect of segmentation: segmented animations were more efficient than continuous animations (i.e., equal test performance with lower investment of mental effort during learning) for students with lower levels of prior knowledge, but not for students with higher levels of prior knowledge. 相似文献
99.
We conducted a quantitative meta-analysis of previous research on the technology acceptance model (TAM) in an attempt to make well-grounded statements on the role of subjective norm. Furthermore, we compared TAM results by taking into account moderating effects of one individual-related factor (type of respondents), one technology-related factor (type of technology), and one contingent factor (culture). Results indicated a significant influence of subjective norm on perceived usefulness and behavioral intention to use. Moderating effects were found for all three factors. The findings yielded managerial implications for both intra-company and market-based settings. 相似文献
100.
Carlos Raniery Paula dos Santos Jeroen Famaey Jürgen Schönwälder Lisandro Zambenedetti Granville Aiko Pras Filip De Turck 《Journal of Network and Systems Management》2016,24(3):764-787
Network and service management has established itself as a research field in the general area of computer networks. However, up to now, no appropriate organization of the field has been carried out in terms of a comprehensive list of terms and topics. In this paper, we introduce a taxonomy for network and service management. With such a taxonomy, it is possible to better understand the landscape of research as well as to reason about possible future challenges and opportunities. As such, in addition to the taxonomy itself, we also present an initial analysis of the field’s past, present, and future, based on the records of papers submitted and accepted in major conferences in the area, as well as a site survey performed through a questionnaire answered by experts from both industry and academia. 相似文献