A Bayesian model for implicit effects in perceptual identification.

Retrieving effectively from memory (REM; R. M. Shiffrin & M. Steyvers, 1997), an episodic model of memory, is extended to implicit memory phenomena, namely the perceptual identification studies reported in R. Ratcliff and G. McKoon (1997). In those studies, the influence of prior study was greatest when words were presented most briefly and when forced-choice targets and foils were most similar. R. Ratcliff and G. McKoon use these data to argue against models in which prior study changes a word's representation. A model in which prior study changes a word's representation by adding context information is fit to their data; at test, the model uses a Bayesian decision process to compare the perceptual and context features associated with the test flash to stored traces. The effects of prior study are due to matching extra context information and are larger when alternatives share many features, thereby reducing noise that attenuates these effects. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The role of item strength in retrieval-induced forgetting.

In 3 experiments, the role of item strength in the retrieval-induced forgetting paradigm was tested. According to the inhibition theory of forgetting proposed by M. C. Anderson, R. A. Bjork, and E. L. Bjork (1994), retrieval-induced forgetting should be larger for items that are more strongly associated with the category cue. In the present experiments, the authors varied item strength on the study list by manipulating the position of an item within its category (Experiments 1 and 2) and by the number of presentations in the study phase (Experiment 3). Contrary to the predictions from inhibition theory, in all 3 experiments, stronger items did not show more retrieval-induced forgetting than weaker items. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The Common Operational Picture as Collective Sensemaking

The common operational picture is used to overcome coordination and information management problems during emergency response. Increasingly, this approach is incorporated in more advanced information systems. This is rooted in an ‘information warehouse’ perspective, which implies information can be collected, sorted and exchanged in an accessible and univocal form. In practice, however, professionals interpret similar information differently. Therefore, we focus on how emergency responders develop collective sensemaking from information. We employ a ‘trading zone’ perspective, in which information is negotiated, to study information management in an ethnographic study of disaster exercises in the Netherlands. Our analysis shows how professionals attribute different meanings to information that distorts the coordination process. We end by stressing the importance of actionable knowledge and reflexivity.     

Sequential enzymatic quantification of two sugars in a single microchannel

This paper presents the implementation of a multiple analyte enzyme assay, based on the sequential injection of the different enzyme solutions, in an electrokinetic driven microfluidic chip. The assay methodology for the simultaneous quantification of d-glucose and d-fructose was reported in previous publications but here the real integration of both enzyme assays was achieved. When assays were executed separately, good reproducibility was observed with average CV values of 5.2% and 4.5% for the d-glucose and d-fructose assay, respectively. Next, the assays for the quantification of d-glucose and d-fructose were integrated simultaneously on chip, where each assay was executed consecutively in the same microreactor by applying a specific sequence of potentials at the reservoirs. This article proves the integration of a sequential based quantification approach in continuous microfluidic chips with electrokinetic actuation.     

Researching instructional use and the technology acceptation of learning management systems by secondary school teachers

The aim of this large-scale study was to understand the technology acceptation of learning management systems (LMS) by secondary school teachers and to investigate the instructional use of LMS, distinguishing between informational use and communicational use. The predictive model further includes: perceived usefulness, perceived ease of use, subjective norm, personal innovativeness in the domain of information technology, experience and internal ICT support at school level. Data were collected from 505 Flemish secondary school teachers. After performing satisfactory reliability and validity checks, the study was able to support all relationships among the 9 variables. Informational use was found to be a precursor for communicational use, perceived ease of use of the LMS is the strongest predictor in LMS-acceptation. Internal ICT support has a direct effect on the informational use of the LMS and on subjective norm. Implications stress that secondary school managers in education should take into account the importance of a teachers’ efforts and performance perceptions and the direct and indirect impact of internal ICT support on LMS adoption.     

Controlling droplet size variability of a digital lab-on-a-chip for improved bio-assay performance

Although several applications of electrowetting on dielectric digital lab-on-a-chips are reported in literature, there is still a lack of knowledge about the influence of operational and design parameters on the performance of an analytical assay. This paper investigates how droplet size variability, introduced by droplet dispensing and splitting, influences the assay performance with respect to repeatability and accuracy and presents a novel method to reduce this variability. Both a theoretical and experimental approach were followed. Monte Carlo simulations were applied to study the cumulative effect of the variability caused by different droplet manipulations on the final assay performance. It is shown that a highly controllable droplet generation and manipulation is achieved with respect to droplet size variability through an accurate control of actuation voltage, activation time, relaxation time, and electrode size. As a case study, it is illustrated that through optimization of these parameters a complete on-chip calibration curve is obtained for a d-glucose assay with an average CV-value of 2%. These new insights aim to bring the digital lab-on-a-chip technology closer to researchers in the field of diagnostics offering them a valuable and accessible alternative to standard analysis platforms.     

Connect & Drive: design and evaluation of cooperative adaptive cruise control for congestion reduction

Road throughput can be increased by driving at small inter-vehicle time gaps. The amplification of velocity disturbances in upstream direction, however, poses limitations to the minimum feasible time gap. This effect is covered by the notion of string stability. String-stable behavior is thus considered an essential requirement for the design of automatic distance control systems, which are needed to allow for safe driving at time gaps well below 1 s. Using wireless inter-vehicle communications to provide real-time information of the preceding vehicle, in addition to the information obtained by common Adaptive Cruise Control (ACC) sensors, appears to significantly decrease the feasible time gap, which is shown by practical experiments with a test fleet consisting of six passenger vehicles. The large-scale deployment of this system, known as Cooperative ACC (CACC), however, poses challenges with respect to the reliability of the wireless communication system. A solution for this scalability problem can be found in decreasing the transmission power and/or beaconing rate, or adapting the communications protocol. Although the main CACC objective is to increase road throughput, the first commercial application of CACC is foreseen to be in truck platooning, since short distance following is expected to yield significant fuel savings in this case.

     

An efficient method for the synthesis of peptide aldehyde libraries employed in the discovery of reversible SARS coronavirus main protease (SARS-CoV Mpro) inhibitors

A method for the parallel solid-phase synthesis of peptide aldehydes has been developed. Protected amino acid aldehydes obtained by the racemization-free oxidation of amino alcohols with Dess-Martin periodinane were immobilized on threonyl resins as oxazolidines. Following Boc protection of the ring nitrogen to yield the N-protected oxazolidine linker, peptide synthesis was performed efficiently on this resin. A peptide aldehyde library was designed for targeting the SARS coronavirus main protease, SARS-CoV M(pro)(also known as 3CL(pro)), on the basis of three different reported binding modes and supported by virtual screening. A set of 25 peptide aldehydes was prepared by this method and investigated in inhibition assays against SARS-CoV M(pro). Several potent inhibitors were found with IC(50) values in the low micromolar range. An IC(50) of 7.5 muM was found for AcNSTSQ-H and AcESTLQ-H. Interestingly, the most potent inhibitors seem to bind to SARS-CoV M(pro) in a noncanonical binding mode.     

A Nanochannel Platform for Single DNA Studies: From Crowding,Protein DNA Interaction,to Sequencing of Genomic Information

The study of nanochannel-confined DNA is important from biotechnological and biophysical points of view. We produce nanochannels in elastomer with soft lithography and proton beam writing. Issues concerning DNA confined in such quasi one-dimensional channels are discussed. We describe DNA stretching via the control of channel diameter and buffer conditions and how the extension can be interpreted with theory and computer simulation. We then discuss the conformation of nano-confined DNA crowded by neutral polymers and like-charged proteins. As an example of a protein that has an affinity to DNA, the effect of heat-stable nucleoid-structuring protein, H-NS, on the folding and compaction of DNA is reviewed. Compaction of DNA by eukaryotic protamine and unpacking of pre-compacted DNA through an increase in salt concentration are discussed. We review results obtained with a novel, cross-channel device that allows the monitoring of the dynamic, conformational response of DNA after exposure to a ligand or protein and/or a change in buffer conditions in situ. As a biotechnological application, linearization of DNA by bottlebrush coating with a polypeptide copolymer is discussed. It is demonstrated that large-scale genomic organization can be sequenced using single DNA molecules on an array of elastomeric nanochannels. Overall, our results show that the effects of ligands and proteins on the conformation, folding, and condensation of DNA are not only related to classical controlling factors, such as osmotic pressure, charge, and binding, but that the interplay with confinement in a nanospace is of paramount importance.     

An Improved Technique for the Rapid Chemical Characterisation of Bacterial Terpene Cyclases

A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli. The vector was used for the expression and chemical characterisation of three bacterial terpene cyclases.