Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

Colibacillosis is an acute and chronic avian disease caused by avian pathogenic E. coli (APEC). Previous studies have demonstrated that RIP2 plays a significant role in APEC infection. Moreover, increasing evidence indicates that microRNAs (miRNAs) are involved in host–pathogen interactions and the immune response. However, the role of miRNAs in the host against APEC infection remains unclear. Herein, we attempted to reveal new miRNAs potentially involved in the regulation of the immune and inflammatory response against APEC infection, with a particular focus on those possibly correlated with RIP2 expression, via miRNA-seq, RT-qPCR, Western blotting, dual-luciferase reporter assay, and CCK-8. The results showed that a total of 93 and 148 differentially expressed (DE) miRNAs were identified in the knockdown of RIP2 cells following APEC infection (shRIP2+APEC) vs. knockdown of RIP2 cells (shRIP2) and shRIP2 vs. wild-type cells (WT), respectively. Among those identified DE miRNAs, the biological function of gga-miR-455-5p was investigated. It was found that gga-miR-455-5p regulated by RIP2 was involved in the immune and inflammatory response against APEC infection via targeting of IRF2 to modulate the expression of type I interferons. Additionally, RIP2 could directly regulate the production of the type I interferons. Altogether, these findings highlighted the crucial role of miRNAs, especially gga-miR-455-5p, in host defense against APEC infection.     

Wire-in-Wire TiO2/C Nanofibers Free-Standing Anodes for Li-Ion and K-Ion Batteries with Long Cycling Stability and High Capacity

Wearable and portable mobile phones play a critical role in the market,and one of the key technologies is the flexible electrode with high spe-cific capacity an...     

BOPP薄膜市场现状及发展趋势

介绍了我国双向拉伸聚丙烯薄膜的市场现状,并对其发展趋势进行了展望。     

Collaborative Multiple Access And Energy-Efficient Resource Allocation in Distributed Maritime Wireless Networks

With the rapid increasing of maritime activities, maritime wireless networks(MWNs) with high reliability, high energy efficiency, and low delay are required. However, the centralized networking with fixed resource scheduling is not suitable for MWNs due to the special environment. In this paper,we introduce the collaborative relay communication in distributed MWNs to improve the link reliability, and propose an orthogonal time-frequency resource block reservation based multiple access(RRMA) sche...     

S波段微波照射对大鼠免疫功能的影响

用S波段高功率微波照射大鼠,观察大鼠白细胞计数、淋巴细胞数、T细胞及其亚群和免疫球蛋白的变化,以期了解S波段高功率微波照射对大鼠免疫功能的影响.结果表明,S波段高功率微波照射可以引起大鼠白细胞水平降低,T细胞亚群出现兴奋效应,免疫球蛋白早期出现不同程度的降低,表现出低剂量刺激而高剂量抑制的效应.     

Fabrication and performance of atmospheric plasma sprayed solid oxide fuel cells with liquid antimony anodes

A solid oxide fuel cell (SOFC) with a liquid antimony anode (LAA) is a potential energy conversion technology for the use of impurity-containing fuels.Atmospher...     

Eventual Clusterer: A Modular Approach to Designing Hierarchical Consensus Protocols in MANETs

This paper proposes a modular approach to the design of hierarchical consensus protocols for the mobile ad hoc network with a static and known set of hosts. A two-layer hierarchy is imposed on the network by grouping mobile hosts into clusters, each with a clusterhead. The messages from and to the hosts in the same cluster are merged/unmerged by the clusterhead so as to reduce the message cost and improve the scalability. The proposed modular approach separates the concerns of clustering hosts from achieving consensus. A clustering function, called eventual clusterer (denoted as diamC), is designed for constructing and maintaining the two-layer hierarchy. Similar to unreliable failure detectors, diamC greatly facilitates the design of hierarchical protocols by providing the fault-tolerant clustering function transparently. We propose an implementation of diamC based on the failure detector diamS. Using diamC, we design a new hierarchical consensus protocol. As shown by the performance evaluation results, the proposed consensus protocol can save both message cost and time cost. Our proposed modular design is therefore effective and can lead to efficient solutions to achieving consensus in mobile ad hoc networks.     

双余度无刷直流电动机驱动电流非均衡状况的研究

双余度无刷直流电动机由于具有较高的安全性和容错性,已广泛应用于电力作动器等关键电力系统中.由于器件以及电机制造上的各种因素,两个电气余度的参数不会完全一致,电流出现不均衡的情况,将导致电机铜损的剧增、散热条件的恶化以及输出转矩的较大波动,影响到双余度电机性能的发挥.通过对双余度无刷直流电动机铜损以及转矩波动的分析,得出了电流不均衡情况下电机铜损变化规律和转矩波动规律,并通过样机验证了理论分析结论.     

动态环境下产品组合策略竞争的博弈分析

建立了对称信息条件下的双寡头垄断企业基于价格和知识水平的组合策略选择的博弈模型,通过对模型稳态均衡解的求解和讨论分析了双方的组合策略选择,从而为企业进行产品组合策略的系统规划提供了理沦上的指导.     

基于LCC理论的软件项目多目标权衡

传统的软件项目成本模型只反映出与工作量有关的费用,对于因质量不足而引起的成本无法度量.将全寿命周期成本(LCC)理论应用于软件项目成本计算,按照分部分项方法建立了软件项目的完全成本模型.在软件项目工期-成本二维权衡模型的基础上,通过设置多级指标体系对软件项目质量进行量化,从而将软件质量纳入权衡目标,在此基础上构建了软件项目工期、成本、质量的多目标权衡离散模型,运用改进的禁忌搜索算法求解模型,以可视化三维曲面输出帕累托非劣解,最后分析了项目决策者获得项目最佳实施模式的途径.