Biocompatible electrospun tactic poly(methyl methacrylate) blend fibers

Uniform and beads free fibers of pristine syndiotactic PMMA (s-PMMA), isotactic PMMA (i-PMMA), and their blends in the ratio of s:i = 3:1, 1:1 and 1:3 were successfully prepared using the electrospinning technique. The tactic PMMA blend fibers showed unique thermal stability and glass transition temperatures compared to their pristine counterparts. An interesting endotherm peak was observed for the s:i = 1:3 electrospun fibers, which might indicate a complex formation between the two tactic PMMAs. Systematic surface functionalities study by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) revealed the interactions between these two tactic PMMAs. Biocompatibility of tactic PMMA and their blend fibers was first time comparably investigated using HeLa as the model mammalian cell line; an intriguing observance was first revealed that the blend fibers showed better biocompatibility than both pristine ones, though the behind mechanism is not well understood yet.     

Sintering Behavior and Dielectric Properties of Ultra‐Low Temperature Fired Silver Molybdate Ceramics

Ag₂MoO₄ ceramic was prepared by using the solid‐state reaction method, which could be sintered at 450°C for 2 h, having a relative permittivity of 8.08, a Qf value of 17 000 GHz, and a temperature coefficient of resonance frequency about ?133 ppm/°C. Ag₂MoO₄ ceramic was chemically compatible with silver but reacted seriously with aluminum to form (Ag_0.5Al_0.5)MoO₄ during the sintering. The fitting of infrared spectra and the Shannon's additive rule were employed to study intrinsic dielectric behaviors of the ceramics at microwave region. Ionic displacive polarization and the electronic polarization contributed almost equally to the dielectric permittivity of the ceramic at microwave region. The Ag₂MoO₄ ceramics could be a good candidate for ultra‐low temperature co‐fired microwave devices.     

环境美学视域下的黔阳古城“居住”解读

环境美学的基本问题是人与自然的关系问题。然对于人具有两种意义：一是资源,二是家园。环境就其本质来说,是人的家园,是生命之本,更是居住之所。在环境美学的视域中,黔阳与人类的“居住”本质,在大地上最为原初的居住之思息息相关。黔阳作为古老的,“曾在的”居住来阐明居住如何才能够建造。“居住”比“筑造/建筑”更为本源,筑造/建筑是为了居住,但居住不是一般意义上的居住,而是“诗意地居住”,与人们的居住所依靠的一切建筑物有关。居住的本质是自然环境的完美性与人居环境的完美性是统一的。     

内陆核电厂液态流出物液转气排放可行性初探

本文针对我国缺乏受纳水体区域核电厂选址和建设的瓶颈问题,以冀东核电厂址和国内某典型内陆厂址为例,对液态流出物液转气排放的源项、工艺及环境影响开展了一些研究工作,从公众剂量评价的角度,初步论证了液转气排放技术路线的可行性,为我国缺乏受纳水体区域核电厂的选址、建设和监管提供参考。     

西石门铁矿尾矿管道压力增高的原因分析及建议

针对西石门铁矿2006年6月选矿生产遇到的难题,从影响尾矿管道压力的因素——入选原矿质量、入选原矿处理量、管道内径变化、尾矿粒度出发,采取现场采样、选矿实验以及理论计算的方法,分析造成西石门铁矿尾矿管道压力增高的原因。以此次实验数据及相关经济技术指标为依据,对实际生产提出合理的建议和措施,进一步指导西石门铁矿选矿系统正常生产。     

青养蛋白抗氧化肽的制备及其胃肠道消化性的研究

采用碱性蛋白酶、木瓜蛋白酶、胰酶、风味酶四种酶水解青养蛋白,探讨了各种酶对青养蛋白的酶解特性、清除DPPH·能力和ORAC值的差异。结果表明,木瓜蛋白酶和风味酶复配酶解青养蛋白可以得到较好的抗氧化活性肽,且水解度、蛋白回收率均较好;酶解产物的氨基酸组成中EAA/TAA为45.06%,EAA/NEAA为82.01%均高于FAO/WHO的推荐值,表明青养蛋白酶解物是较优的营养补充剂,且富含抗氧化性氨基酸;分子量分布表明,大于10ku的组分含量为3%,5~10ku肽段的含量为9%,低于3ku的组分含量达到73%。构建模拟了胃肠道消化,青养多肽的抗氧化活性在0~0.5h之间都有一个显著增加的过程(p<0.05),在2.5~4.0h消化的过程中得到的肽的抗氧化活性均较低,且不同时间段之间的活性差异不显著。      

内容中心网络中能效感知的概率性缓存机制

内置缓存技术是内容中心网络(Content Centric Networking, CCN)的核心技术之一。现有的研究大多主要针对网络资源利用率的优化,而忽略了网络能耗的问题。该文首先建立了一个能耗模型对CCN的网络能耗进行分析,并设计了一个能效判决条件来优化缓存过程的能效性。进而,在此基础上综合考虑内容流行度和节点中心性等因素提出一种能效感知的概率性缓存机制(E2APC)。仿真结果表明,该机制能在保证较高的缓存命中率和较小的平均响应跳数的同时有效地降低网络的整体能耗。     

The Role of TKS5 in Chromosome Stability and Bladder Cancer Progression

TKS5 promotes invasion and migration through the formation of invadopodia in some tumour cells, and it also has an important physiological function in cell migration through podosome formation in various nontumour cells. To date, the role of TKS5 in urothelial cells, and its potential role in BC initiation and progression, has not yet been addressed. Moreover, the contribution of TKS5 to ploidy control and chromosome stability has not been reported in previous studies. Therefore, in the present study, we wished to address the following questions: (i) Is TKS5 involved in the ploidy control of urothelial cells? (ii) What is the mechanism that leads to aneuploidy in response to TKS5 knockdown? (iii) Is TKS5 an oncogene or tumour-suppressor gene in the context of BC? (iv) Does TKS5 affect the proliferation, migration and invasion of BC cells? We assessed the gene and protein expressions via qPCR and Western blot analyses in a set of nontumour cell strains (Y235T, HBLAK and UROtsa) and a set of BC cell lines (RT4, T24, UMUC3 and J82). Following the shRNA knockdown in the TKS5-proficient cells and the ectopic TKS5 expression in the cell lines with low/absent TKS5 expression, we performed functional experiments, such as metaphase, invadopodia and gelatine degradation assays. Moreover, we determined the invasion and migration abilities of these genetically modified cells by using the Boyden chamber and wound-healing assays. The TKS5 expression was lower in the bladder cancer cell lines with higher invasive capacities (T24, UMUC3 and J82) compared to the nontumour cell lines from human ureter (Y235T, HBLAK and UROtsa) and the noninvasive BC cell line RT4. The reduced TKS5 expression in the Y235T cells resulted in augmented aneuploidy and impaired cell division. According to the Boyden chamber and wound-healing assays, TKS5 promotes the invasion and migration of bladder cancer cells. According to the present study, TKS5 regulates the migration and invasion processes of bladder cancer (BC) cell lines and plays an important role in genome stability.     

The role of Mitochondrial Fission Proteins in Mitochondrial Dynamics in Kidney Disease

Mitochondria have many forms and can change their shape through fusion and fission of the outer and inner membranes, called “mitochondrial dynamics”. Mitochondrial outer membrane proteins, such as mitochondrial fission protein 1 (FIS1), mitochondrial fission factor (MFF), mitochondrial 98 dynamics proteins of 49 kDa (MiD49), and mitochondrial dynamics proteins of 51 kDa (MiD51), can aggregate at the outer mitochondrial membrane and thus attract Dynamin-related protein 1 (DRP1) from the cytoplasm to the outer mitochondrial membrane, where DRP1 can perform a scissor-like function to cut a complete mitochondrion into two separate mitochondria. Other organelles can promote mitochondrial fission alongside mitochondria. FIS1 plays an important role in mitochondrial–lysosomal contacts, differentiating itself from other mitochondrial-fission-associated proteins. The contact between the two can also induce asymmetric mitochondrial fission. The kidney is a mitochondria-rich organ, requiring large amounts of mitochondria to produce energy for blood circulation and waste elimination. Pathological increases in mitochondrial fission can lead to kidney damage that can be ameliorated by suppressing their excessive fission. This article reviews the current knowledge on the key role of mitochondrial-fission-associated proteins in the pathogenesis of kidney injury and the role of their various post-translational modifications in activation or degradation of fission-associated proteins and targeted drug therapy.