A disposable sensor based on immobilization of acetylcholinesterase to multiwall carbon nanotube modified screen-printed electrode for determination of carbaryl

A simple method has been devised for immobilization of acetylcholinesterase (AChE) covalent bonding to a multiwall carbon nanotube (MWNT)-cross-linked cellulose acetate composite on a screen-printed carbon electrode (SPCE) and a sensitive and disposable amperometric sensor for rapid determination of carbaryl pesticide is proposed. The immobilized enzyme was preserved on this film because of the excellent biocompatibility and non-toxicity of cellulose acetate. Based on the inherent conductive properties of the MWNT, the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl. MWNT improved the interface enzymatic hydrolysis reaction and increased the amperometric response of the sensor. Under optimum conditions, the inhibition of carbaryl on AChE increased linearly with the increasing concentration of carbaryl in two ranges, from 0.01 to 0.5 μg mL⁻¹ and from 2 to 20 μg mL⁻¹, with the correlation coefficients of 0.9985 and 0.9977, respectively. The detection limit was 0.004 μg mL⁻¹ taken as the concentration equivalent to 10% decrease in signal. The sensor showed acceptable stability, accuracy and could be fabricated in batches, thus it is economic and portable. This type of disposable enzyme-based amperometric sensor has extensive application potential in environmental monitoring of pesticides.     

考虑塑性铰外移的钢框架梁柱连接设计

介绍美国联邦突发事件管理局 (FEMA)对 1 994年北岭地震中焊接钢框架节点破坏原因的正式解释 ,说明塑性铰外移的必要性。此外 ,还介绍了塑性铰外移的两种基本形式以及狗骨式 (RBS)连接的有关设计规定和适用条件 ,其设计方法可供我国工程设计人员参考。     

旋风燃烧器冷态模型内颗粒运动轨迹计算

在冷态模型流场内，考虑气流对粉煤颗粒的气动阻力，建立其运动微分方程式。通过计算，得到九种粒径的颗粒在流场中的运动轨迹、停留时间、着膜位置和气－固相相对速度与该燃烧器几何参数和气动参数的关系，为燃烧器的深入研究提出了建议。     

自动加矾控制系统设计和运行

介绍了自动加矾控制系统在厦门安兜水厂的应用情况和运行处理效果     

Failure analysis and wear mechanism study of a heavily loaded gear

Failure analysis and a study of the wear mechanism were performed on a heavily loaded carburized gear with some unusual features. The analysis showed that the different failure forms on the tooth surface of the gear were dependent on the sub-surface shearing stress, the lubrication conditions and the surface roughness. Delaminations formed on the addendum surface were the main reason for the gear failure. A suggestion for improvement has been tried, and a desirable result has been obtained.     

Gas Permeability of Wetwood and Normal Wood of Subalpine Fir in Relation to Drying

Subalpine fir (Abies lasiocarpa Hook) lumber containing wetpockets (wetwood) was used for the measurements of gas permeability and the results were then compared to the gas permeability of normal wood, which was free of the wetpockets. The impacts of pretreatment methods were also investigated, including steaming at green condition, steaming at fiber saturation point (FSP), microwave treatment, and radio-frequency treatment. Results from this study demonstrated that both the transverse and the longitudinal gas permeability of subalpine fir wetwood are greater than that of normal wood. There is no significant change in the permeability after 4-h steaming pretreatment at green condition and at FSP. The impacts of microwave and radio-frequency pretreatments were not significant, but the findings cannot be generalized because they may depend on the power intensity applied.     

酞菁光敏剂对S180移植瘤的疗效

研究了GaS_4PC,ZnS_2PC两种酞菁在激光照射下对S180肉瘤的疗效,并讨论了其他酞菁光敏剂对癌细胞的杀伤效果。     

天津市正常人全血和血清微量元素含量分析

人体内微量元素含量平衡与否与人体健康状态有着密切关系已为大量实践所证实。人体血液中微量元素的正常值测定是研究人体健康与微量元素关系的一项基础性工作,本文提供了天津市正常人全血及血清中几种微量元素含量的数据。     

RETRACTED ARTICLE: Research on image classification method based on convolutional neural network

Neural Computing and Applications - Image classification method is currently the more popular image technology, but it still has certain problems in practice. In order to improve the image...     

17-4PH沉淀硬化不锈钢氮离子注入及其复合改性的研究

采用N+注入，N-C-O共渗结合N+注入的表面复合改性方法，探索强化17－4PH钢表面的有效途径，该方法赋予基体表面一定深度的改性范围与低摩擦系数，因而强化效果达到最佳。同时还探索了不同时效温度下，基体心部硬度变化的规律。