Isomeric diacylglycerols – Determination of 1,2‐ and 1,3‐diacylglycerols in virgin olive oil

The “Joint Committee for the Analysis of Fats, Oils, Fatty Products, Related Products and Raw Materials (GA Fett)” has developed the following method for the determination of isomeric diacylglycerols in virgin olive oils to detect the freshness of oils. It is intended to include this method in Section C, Chapter VI of the German Standard Methods.*     

Towards Automatic Creation of Realistic Anthropomorphic Models for Realtime 3D Telecommunication

This contribution describes the creation of highly realistic 3D models of participants for distributed 3D videoconferencing systems. These models consist of a flexible triangular mesh surrounding an interior skeleton structure, which is based on a simplified human skeleton. The vertices of the predefined mesh template are arranged in rigid rings along the bones of the skeleton. Using 3D data obtained by a stereoscopic approach the size and shape of this initial mesh is adapted to the real person. Adaptation allows to texture the model from real images leading to a natural impression. The mesh organization in rigid rings gives an efficient way for surface deformation according to the skeleton movements. The resulting model is transmitted once and subsequently animated using the simple parameter set of the interior skeleton structure.     

Using 15N‐Ammonium to Characterise and Map Potassium Binding Sites in Proteins by NMR Spectroscopy

A variety of enzymes are activated by the binding of potassium ions. The potassium binding sites of these enzymes are very specific, but ammonium ions can often replace potassium ions in vitro because of their similar ionic radii. In these cases, ammonium can be used as a proxy for potassium to characterise potassium binding sites in enzymes: the ¹H,¹⁵N spin‐pair of enzyme‐bound ¹⁵NH₄⁺ can be probed by ¹⁵N‐edited heteronuclear NMR experiments. Here, we demonstrate the use of NMR spectroscopy to characterise binding of ammonium ions to two different enzymes: human histone deacetylase 8 (HDAC8), which is activated allosterically by potassium, and the bacterial Hsp70 homologue DnaK, for which potassium is an integral part of the active site. Ammonium activates both enzymes in a similar way to potassium, thus supporting this non‐invasive approach. Furthermore, we present an approach to map the observed binding site onto the structure of HDAC8. Our method for mapping the binding site is general and does not require chemical shift assignment of the enzyme resonances.     

Floc volume effects in suspensions and its relevance for wastewater engineering

The aim of this paper is to better understand oxygen transfer reduction caused by floc suspensions. We demonstrate that the overall floc volume significantly influences oxygen transfer depletion. Submerged fine bubble and coarse bubble diffusers are affected in the same way by this phenomenon. The mixed liquor suspended solids concentration (MLSS concentration) is not an appropriate parameter for describing or relating phenomena that are caused by the overall floc volume in activated sludge (e.g., oxygen transfer depression and sludge sedimentation characteristics). A better correlation is achieved by using the mixed liquor volatile suspended solids concentration (MLVSS concentration). To characterize the effects of the overall floc volume in suspensions whose MLVSS concentration cannot be determined (e.g., inorganic iron hydroxide flocs), a new method-the hydrostatic floc volume (HFV)-that approximates the overall floc volume in floc suspensions is introduced. Application of this method demonstrates that oxygen transfer depression caused by iron hydroxide flocs and activated sludge flocs is similar.     

Triggering the volume phase transition of core-shell Au nanorod-microgel nanocomposites with light

We have coated gold nanorods (NRs) with thermoresponsive microgel shells based on poly(N-isopropylacrylamide) (pNIPAM). We demonstrate by simultaneous laser-heating and optical extinction measurements that the Au NR cores can be simultaneously used as fast optothermal manipulators (switchers) and sensitive optical reporters of the microgel state in a fully externally controlled and reversible manner. We support our results with optical modeling based on the boundary element method and 3D numerical analysis on the temperature distribution. Briefly, we show that due to the sharp increase in refractive index resulting from the optothermally triggered microgel collapse, the longitudinal plasmon band of the coated Au NRs is significantly red-shifted. The optothermal control over the pNIPAM shell, and thereby over the optical response of the nanocomposite, is fully reversible and can be simply controlled by switching on and off a NIR heating laser. In contrast to bulk solution heating, we demonstrate that light-triggering does not compromise colloidal stability, which is of primary importance for the ultimate utilization of these types of nanocomposites as remotely controlled optomechanical actuators, for applications spanning from drug delivery to photonic crystals and nanoscale motion.     

Reduction of a detailed reaction mechanism for hydrogen combustion under gas turbine conditions

The aim of this study is to find a reduced mechanism that accurately represents chemical kinetics for lean hydrogen combustion at elevated pressures, as present in a typical gas turbine combustor. Calculations of autoignition, extinction, and laminar premixed flames are used to identify the most relevant species and reactions and to compare the results of several reduced mechanisms with those of a detailed reaction mechanism. The investigations show that the species OH and H are generally the radicals with the highest concentrations, followed by the O radical. However, the accumulation of the radical pool in autoignition is dominated by HO₂ for temperatures above, and by H₂O₂ below the crossover temperature. The influence of H₂O₂ reactions is negligible for laminar flames and extinction, but becomes significant for autoignition. At least 11 elementary reactions are necessary for a satisfactory prediction of the processes of ignition, extinction, and laminar flame propagation under gas turbine conditions. A 4-step reduced mechanism using steady-state approximations for HO₂ and H₂O₂ yields good results for laminar flame speed and extinction limits, but fails to predict ignition delay at low temperatures. A further reduction to three steps using a steady-state approximation for O leads to significant errors in the prediction of the laminar flame speed and extinction limit.     

Evaluation of image-based modeling and laser scanning accuracy for emerging automated performance monitoring techniques

Accurate and rapid assessment of the as-built status on any construction site provides the opportunity to understand the current performance of a project easily and quickly. Rapid project assessment further identifies discrepancies between the as-built and as-planned progress, and facilitates decision making on the necessary remedial actions. Currently, manual visual observations and surveying are the most dominant data capturing techniques but they are time-consuming, error-prone, and infrequent, making quick and reliable decision-making difficult. Therefore, research on new approaches that allow automatic recognition of as-built performance and visualization of construction progress is essential. This paper presents and compares two methods for obtaining point cloud models for detection and visualization of as-built status for construction projects: (1) A new method of automated image-based reconstruction and modeling of the as-built project status using unordered daily construction photo collections through analysis of Structure from Motion (SfM); (2) 3D laser scanning and analysis of the as-built dense point cloud models. These approaches provide robust means for recognition of progress, productivity, and quality on a construction site. In this paper, an overview of the newly developed automated image-based reconstruction approach and exclusive features which distinct it from other image-based or conventional photogrammetric techniques is presented. Subsequently the terrestrial laser scanning approach carried out for reconstruction and comparison of as-built scenes is presented. Finally the accuracy and usability of both of these techniques for metric reconstruction, automated production of point cloud models, 3D CAD shape modeling, and as-built visualizations is evaluated and compared on eight different case studies. It is shown that for precise defect detection or alignment tasks, image-based point cloud models may not be as accurate and dense as laser scanners' point cloud models. Nonetheless image-based point cloud models provide an opportunity to extract as-built semantic information (i.e., progress, productivity, quality and safety) through the content of the images, are easy to use, and do not need add burden on the project management teams by requiring expertise for data collection or analysis. Finally image-based reconstruction automatically provides photo alignment with point cloud models and enables image-based renderings which can remarkably impact automated performance monitoring and as-built visualizations.     

Leaching of a zinc ore and concentrate using the Geocoat™ technology

In this study, the Geocoat™ technology was used for the extraction of zinc from a mineral concentrate obtained from the Kooshk mine (Yazd, Iran) by a culture dominated by the mesophilic bacterium Acidithiobacillusferrooxidans in a packed column bioreactor. A low grade sphalerite ore was used as support for the concentrate coating. During the 100 days of leaching pH, Fe³⁺, Fe^total, microbial population density and zinc extraction were measured. The final zinc extraction from concentrate and low grade support was 97% and 78%, respectively, and it was found that leaching from the support does not proceed significantly before leaching from the coating is completed.