Induction of neutralizing antibody in mice by immunization with recombinant 56 kDa protein of Orientia tsutsugamushi

Anti-oriential antibody inhibits Orientia tsutsugamushi attachment to, and penetration of, host cells. However, O. tsutsugamushi antigens that induce the production of a neutralizing antibody have not been identified. The authors immunized mice and rabbits with the recombinant 56 kDa protein of O. tsutsugamushi fused to the maltose binding protein of Escherichia coli (MBP-Bor56) and analysed their effect on O. tsutsugamushi attachment to or penetration of L929 cells. O. tsutsugamushi attachment and penetration were measured by using an indirect immunofluorescent antibody assay (IFA). O. tsutsugamushi growth in L929 cells was determined by [3H]thymidine uptake assay. By IFA, we observed a 96% reduction of attachment or penetration of O. tsutsugamushi treated with rabbit anti-MBP-Bor56 sera. [3H]thymidine uptake showed that mouse anti-MBP-Bor56 sera caused a 91% reduction in O. tsutsugamushi growth, when compared to mouse anti-MBP sera. These results suggest that the 56 kDa protein of O. tsutsugamushi plays an important role in O. tsutsugamushi attachment to or penetration of cells.     

Electrochemical detection of ascorbic acid (vitamin C) using a glassy carbon electrode

A method for the detection of ascorbic acid (AA) was developed using square wave anodic stripping voltammetric (SWASV) analysis with a glassy carbon electrode (GCE). The results indicated a sensitive oxidation peak current of AA on the GCE. A linear curve was obtained within a concentration range of 1-130.0 microg/L with a preconcentration time of 270 s. The relative standard deviation of 30.0 microg/L observed was 0.1016% (n = 12) under optimum conditions. The low detection limit (S/N) was pegged at 0.30 microg/L. Results showed that the method developed can be used to assay biological and pharmaceutical samples, and food samples, as well as other materials requiring AA analyses.     

Characterization of the chemical conversion films that form on Mg−Al alloy in colloidal silica solution

Chemical conversion treatment of Mg−Al alloy (AZ91) using colloidal silica as an alternative to chromate conversion was investigated as a function of solution pH, temperature, solution conditions, and treatment time. The solution used for the colloidal silica coating consisted of colloidal silica, titanium sulfate, and cobalt ions to maintain good anti-corrosion and adhesion properties. Adding CoSO₄ to the colloidal silica solution enhanced the adhesion force between the silica film and magnesium substrate. The optimum conditions for the chemical conversion treatment solution were pH 2, 90-sec treatment, and 25°C.     

Block-based neural networks for personalized ECG signal classification.

This paper presents evolvable block-based neural networks (BbNNs) for personalized ECG heartbeat pattern classification. A BbNN consists of a 2-D array of modular component NNs with flexible structures and internal configurations that can be implemented using reconfigurable digital hardware such as field-programmable gate arrays (FPGAs). Signal flow between the blocks determines the internal configuration of a block as well as the overall structure of the BbNN. Network structure and the weights are optimized using local gradient-based search and evolutionary operators with the rates changing adaptively according to their effectiveness in the previous evolution period. Such adaptive operator rate update scheme ensures higher fitness on average compared to predetermined fixed operator rates. The Hermite transform coefficients and the time interval between two neighboring R-peaks of ECG signals are used as inputs to the BbNN. A BbNN optimized with the proposed evolutionary algorithm (EA) makes a personalized heartbeat pattern classifier that copes with changing operating environments caused by individual difference and time-varying characteristics of ECG signals. Simulation results using the Massachusetts Institute of Technology/Beth Israel Hospital (MIT-BIH) arrhythmia database demonstrate high average detection accuracies of ventricular ectopic beats (98.1%) and supraventricular ectopic beats (96.6%) patterns for heartbeat monitoring, being a significant improvement over previously reported electrocardiogram (ECG) classification results.     

A fast-running core prediction model based on neural networks for load-following operations in a soluble boron-free reactor

A fast prediction model for load-following operations in a soluble boron-free reactor has been proposed, which can predict the core status when three or more control rod groups are moved at a time. This prediction model consists of two multilayer feedforward neural network models to retrieve the axial offset and the reactivity, and compensation models to compensate for the reactivity and axial offset arising from the xenon transient. The neural network training data were generated by taking various overlaps among the control rod groups into consideration for training the neural network models, and the accuracy of the constructed neural network models was verified. Validation results of predicting load following operations for a soluble boron-free reactor show that this model has a good capability to predict the positions of the control rods for sustaining the criticality of a core during load-following operations to ensure that the tolerable axial offset band is not exceeded and it can provide enough corresponding time for the operators to take the necessary actions to prevent a deviation from the tolerable operating band.     

Study of the Reliability Impact of Chlorine Precursor Residues in Thin Atomic-Layer-Deposited HfO2 Layers

Atomic layer deposition (ALD) with HfCl₄ as a precursor is widely used for HfO₂ fabrication. Due to the nature of the precursor under study, i.e., HfCl₄ and H₂O, the presence of chlorine residues in the film due to insufficient hydrolysis is eminent. Obviously, the chlorine residue in the HfO₂ film is suspected to affect the quality of the HfO₂ film. In this paper, The authors reduced the concentration of chlorine residues by increasing the H₂O oxidant pulse time in between the deposition cycles from 0.3 to 10 and 90 s. Time-of-flight secondary ion mass spectrometry analysis shows that this decreases the chlorine concentration in the HfO₂ film by more than one order of magnitude. However, time-dependent dielectric breakdown analysis shows that the lifetime remains quasi unaffected (within identical error bars) for the different injection cycles. Charge pumping analysis was done by varying both pulse frequency and amplitude to investigate the creation of defects, but negligible differences were observed. Therefore, the presence of chlorine residues has no significant impact on the trap generation and reliability of ALD HfO₂ layers, and this result corresponded with the mobility result. The experimental picture is confirmed with first-principle calculations that show that the presence of chlorine residues does not induce defect levels in the bandgap of HfO₂     

Consideration of the Actual Current-Spreading Length of GaN-Based Light-Emitting Diodes for High-Efficiency Design

Based on the proposed experimental method, the current spreading length of GaN-based light-emitting diodes (LEDs) was measured and analyzed for practical device design. In this study, Thompson's and Guo's models, which are categorized according to vertical series resistance (in particular, p-type contact resistance), were used to extract device parameters. It was shown that the measured current spreading length strongly depends on the injected current density. For LEDs fabricated with low-resistance p-type contacts, this behavior could be explained in terms of the accelerated current crowding with higher current densities occurring as a result of the reduced voltage drop across the junction, which is in good agreement with Thompson's relation. However, for LEDs fabricated with high-resistance p-contacts, unlike Guo's prediction, the measured current spreading length also showed a strong dependence on the injected current density. This was attributed to thermal heating at the p-contact, resulting in the reduction of the voltage drop across the p-contact and so junction voltage, which is also in agreement with Thompson's model. Based on the measured parameters and the design rule, efficient p-type reflectors, namely, hybrid reflectors were designed. Compared with conventional ones, LEDs fabricated with the hybrid reflectors exhibited better output power at a reasonable forward voltage, indicating that the proposed method is effective in understanding the actual current spreading and hence the practical design of high-efficiency LEDs.     

Binder MIMO Channels

This paper introduces a multiple-input multiple-output channel model for the characterization of a binder of telephone lines. This model is based on multiconductor transmission line theory, and uses parameters that can be obtained from electromagnetic theory or measured data. The model generates frequency-dependent channel/binder transfer function matrices as a function of cable type, geometric line-spacing and twist-length parameters, and source--load configurations. The model allows the extraction of the magnitude and the phase of individual near end crosstalk, far end crosstalk, split-pair, and phantom transfer functions from the transfer function matrix of the binder. These individual crosstalk transfer functions are often found to be very sensitive to small imperfections in the binder. Examples of category 3 twisted pair American telephone lines and ldquoquadrdquo telephone cables are also presented.     

TD‐CFIE Formulation for Transient Electromagnetic Scattering from 3‐D Dielectric Objects

In this paper, we present a time domain combined field integral equation formulation (TD‐CFIE) to analyze the transient electromagnetic response from dielectric objects. The solution method is based on the method of moments which involves separate spatial and temporal testing procedures. A set of the RWG functions is used for spatial expansion of the equivalent electric and magnetic current densities, and a combination of RWG and its orthogonal component is used for spatial testing. The time domain unknowns are approximated by a set of orthonormal basis functions derived from the Laguerre polynomials. These basis functions are also used for temporal testing. Use of this temporal expansion function characterizing the time variable makes it possible to handle the time derivative terms in the integral equation and decouples the space‐time continuum in an analytic fashion. Numerical results computed by the proposed formulation are compared with the solutions of the frequency domain combined field integral equation.