全文获取类型
收费全文 | 83篇 |
免费 | 6篇 |
专业分类
化学工业 | 11篇 |
金属工艺 | 4篇 |
建筑科学 | 1篇 |
轻工业 | 2篇 |
无线电 | 15篇 |
一般工业技术 | 9篇 |
冶金工业 | 25篇 |
自动化技术 | 22篇 |
出版年
2022年 | 1篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2016年 | 3篇 |
2015年 | 1篇 |
2014年 | 9篇 |
2013年 | 5篇 |
2012年 | 3篇 |
2011年 | 1篇 |
2010年 | 5篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 2篇 |
2006年 | 1篇 |
2005年 | 1篇 |
2004年 | 1篇 |
2003年 | 3篇 |
2002年 | 1篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 8篇 |
1997年 | 8篇 |
1996年 | 2篇 |
1995年 | 6篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有89条查询结果,搜索用时 31 毫秒
51.
N Jourdan M Maurice D Delautier AM Quero AL Servin G Trugnan 《Canadian Metallurgical Quarterly》1997,71(11):8268-8278
Rotaviruses are nonenveloped viruses that infect enterocytes of the small intestine and cause severe infantile gastroenteritis. It was previously thought that rotavirus exits cells by lysis, but this behavior does not match the local pathogenesis of the virus. In this study, we have investigated the release of the simian rotavirus strain (RRV) from the polarized intestinal Caco-2 cells. We found that RRV is released almost exclusively from the apical pole of Caco-2 cells before any cells lyse. Using confocal laser scanning microscopy and drugs that inhibit vesicular transport, we studied the RRV transport route from the endoplasmic reticulum (ER) to the apical side of intestinal cells. We demonstrated that RRV exits from the ER through a carbonyl cyanide m-chlorophenylhydrazone-sensitive vesicular transport. RRV staining was never found within the Golgi apparatus or lysosomes, suggesting that the RRV intracellular pathway does not involve these organelles. This finding was confirmed by treatment with monensin or NH4Cl, which do not affect release of RRV. Electron microscopic analysis revealed RRV containing small smooth vesicles in the apical area and free virions outside the cell in the brush border, consistent with a vesicular vectorial transport of virus. These results may provide, for the first time, a cellular explanation of the pathogenesis of rotavirus. 相似文献
52.
Ratovelomanana F. Vodjdani N. Enard A. Glastre G. Rondi D. Blondeau R. Joergensen C. Durhuus T. Mikkelsen B. Stubkjaer K.E. Jourdan A. Soulage G. 《Photonics Technology Letters, IEEE》1995,7(9):992-994
An all-optical wavelength-converter based on monolithic integration of a Mach-Zehnder interferometer and integrated semiconductor optical amplifiers was demonstrated. This device exhibited high stability, penalty-free conversion at 5 Gbit/s (at a BER of 109), no-excess penalty after a transmission over 60 km on standard fiber, and low signal-polarization dependency. The device operated in a 26-nm-wide optical window 相似文献
53.
54.
Influence of Pre‐organised Architecture on Cell Adhesion by Using Multivalent RGD Compounds
下载免费PDF全文
![点击此处可从《Chembiochem : a European journal of chemical biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Dr. Adrien Grassin Dr. Muriel Jourdan Prof. Pascal Dumy Dr. Didier Boturyn 《Chembiochem : a European journal of chemical biology》2016,17(6):515-520
Multimeric RGD‐containing compounds were designed to study the influence of ligand architecture on binding avidity. We report the synthesis of a series of tetrameric RGD compounds and their in vitro biological evaluation. The further application of molecular dynamic simulations for structural studies of RGD derivatives reveals that the observed affinities correlate with the accessibility of segregated RGD motif. 相似文献
55.
Piezoresistance of top-down suspended Si nanowires 总被引:1,自引:0,他引:1
Koumela A Mercier D Dupré C Jourdan G Marcoux C Ollier E Purcell ST Duraffourg L 《Nanotechnology》2011,22(39):395701
Measurements of the gauge factor of suspended, top-down silicon nanowires are presented. The nanowires are fabricated with a CMOS compatible process and with doping concentrations ranging from 2 × 10(20) down to 5 × 10(17) cm(-3). The extracted gauge factors are compared with results on identical non-suspended nanowires and with state-of-the-art results. An increase of the gauge factor after suspension is demonstrated. For the low doped nanowires a value of 235 is measured. Particular attention was paid throughout the experiments to distinguishing real resistance change due to strain modulation from resistance fluctuations due to charge trapping. Furthermore, a numerical model correlating surface charge density with the gauge factor is presented. Comparison of the simulations with experimental measurements shows the validity of this approach. These results contribute to a deeper understanding of the piezoresistive effect in Si nanowires. 相似文献
56.
N Jourdan JP Brunet C Sapin A Blais J Cotte-Laffitte F Forestier AM Quero G Trugnan AL Servin 《Canadian Metallurgical Quarterly》1998,72(9):7228-7236
Rotavirus infection is the most common cause of severe infantile gastroenteritis worldwide. These viruses infect mature enterocytes of the small intestine and cause structural and functional damage, including a reduction in disaccharidase activity. It was previously hypothesized that reduced disaccharidase activity resulted from the destruction of rotavirus-infected enterocytes at the villus tips. However, this pathophysiological model cannot explain situations in which low disaccharidase activity is observed when rotavirus-infected intestine exhibits few, if any, histopathologic changes. In a previous study, we demonstrated that the simian rotavirus strain RRV replicated in and was released from human enterocyte-like Caco-2 cells without cell destruction (N. Jourdan, M. Maurice, D. Delautier, A. M. Quero, A. L. Servin, and G. Trugnan, J. Virol. 71:8268-8278, 1997). In the present study, to reinvestigate disaccharidase expression during rotavirus infection, we studied sucrase-isomaltase (SI) in RRV-infected Caco-2 cells. We showed that SI activity and apical expression were specifically and selectively decreased by RRV infection without apparent cell destruction. Using pulse-chase experiments and cell surface biotinylation, we demonstrated that RRV infection did not affect SI biosynthesis, maturation, or stability but induced the blockade of SI transport to the brush border. Using confocal laser scanning microscopy, we showed that RRV infection induces important alterations of the cytoskeleton that correlate with decreased SI apical surface expression. These results lead us to propose an alternate model to explain the pathophysiology associated with rotavirus infection. 相似文献
57.
Chiaroni D. Lavigne B. Jourdan A. Sotom M. Hamon L. Chauzat C. Jacquinot J.-C. Barroso A. Zami T. Dorgeuille F. Janz C. Emery J.-Y. Grard E. Renaud M. 《Lightwave Technology, Journal of》1998,16(12):2255-2264
The large growth of telecommunication traffic demand generated by multiple new applications and expected to last at least for the next decade will force telecom operators to consider offering more flexible transport services. All-optical packet switching is a powerful technique to provide this flexibility and to support in a cost-efficient way a wide range of bandwidth consuming applications. After a very brief introduction about the packet-switched network architecture studied in the framework of the ACTS KEOPS project, we describe the structure of the packet-switching node we have defined. We then move into physical and logical analysis of the network including more than 40 network sections based on 160 Gb/s throughput optical packet switching nodes could operate error free. In addition, logical simulations have proved that such networks could provide a quality of service (packet loss rate and packet transfer delay per node) compatible with a large variety of service classes. Both results validate the feasibility of the network concept and pace the way toward a flexible network based on all-optical switching techniques 相似文献
58.
Synthesis,Anti‐tubulin and Antiproliferative SAR of Steroidomimetic Dihydroisoquinolinones
下载免费PDF全文
![点击此处可从《ChemMedChem》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Dr. Mathew P. Leese Dr. Fabrice L. Jourdan Dr. Meriel R. Major Dr. Wolfgang Dohle Dr. Mark P. Thomas Dr. Ernest Hamel Dr. Eric Ferrandis Dr. Mary F. Mahon Dr. Simon P. Newman Dr. Atul Purohit Prof. Dr. Barry V. L. Potter 《ChemMedChem》2014,9(4):798-812
A SAR translation strategy adopted for the discovery of tetrahydroisoquinolinone (THIQ)‐based steroidomimetic microtubule disruptors has been extended to dihydroisoquinolinone (DHIQ)‐based compounds. A steroid A,B‐ring‐mimicking DHIQ core was connected to methoxyaryl D‐ring mimics through methylene, carbonyl, and sulfonyl linkers, and the resulting compounds were evaluated against two cancer cell lines. The carbonyl‐linked DHIQs in particular exhibit significant in vitro antiproliferative activities (e.g., 6‐hydroxy‐7‐methoxy‐2‐(3,4,5‐trimethoxybenzoyl)‐3,4‐dihydroisoquinolin‐1(2H)‐one ( 16 g ): GI50 51 nM in DU‐145 cells). The broad anticancer activity of DHIQ 16 g was confirmed in the NCI 60‐cell line assay giving a mean activity of 33 nM . Furthermore, 6‐hydroxy‐2‐(3,5‐dimethoxybenzoyl)‐7‐methoxy‐3,4‐dihydroisoquinolin‐1(2H)‐one ( 16 f ) and 16 g and their sulfamate derivatives 17 f and 17 g (2‐(3,5‐dimethoxybenzoyl)‐7‐methoxy‐6‐sulfamoyloxy‐3,4‐dihydroisoquinolin‐1(2H)‐one and 7‐methoxy‐2‐(3,4,5‐trimethoxybenzoyl)‐6‐sulfamoyloxy‐3,4‐dihydroisoquinolin‐1(2H)‐one, respectively) show excellent activity against the polymerization of tubulin, close to that of the clinical combretastatin A‐4, and bind competitively at the colchicine binding site of tubulin. Compounds 16 f and 17 f were also shown to demonstrate in vitro anti‐angiogenic activity. Additionally, X‐ray and computational analyses of 17 f reveal that electrostatic repulsion between the two adjacent carbonyl groups, through conformational biasing, dictates the adoption of a “steroid‐like” conformation that may partially explain the excellent in vitro activities. 相似文献
59.
ML Jourdan E Stephanov G Body A Goudeau F Fetissof 《Canadian Metallurgical Quarterly》1993,41(3-4):149-158
Seventy seven biopsy samples of cervical mucosa were tested for the presence of human papillomavirus (HPV) by immunohistochemistry and in situ hybridization. From the 38 samples identified as condyloma or cervical intraepithelial neoplasia (CIN), 31 were positive after in situ hybridization and 14 after immunochemical analysis. HPV 6 was found exclusively in condyloma acuminata (2 samples) whereas the HPV 16 probe essentially hybridized with high grade intraepithelial lesions (CIN II, CIN III). Low grade intraepithelial lesions (flat condyloma, CIN I) demonstrated a larger diversity of HPV types (HPV 16, 18, 31, 33). A close correlation was demonstrated between the histologic features of lesions and their HPV 6 or HPV 31 content but not for other HPV types. HPV 31 containing lesions showed a peculiar architecture with numerous, elongated papillae resulting in a spiked appearance. 相似文献
60.