The influence of summer seasonal extremes on dissolved organic carbon export from a boreal peatland catchment: evidence from one dry and one wet growing season

A small peatland catchment in eastern Finland was monitored for runoff and export of dissolved organic carbon. To exemplify the response of a peatland under years of different meteorological conditions, data from one very dry and one wet growing season are presented. Runoff was 194 and 387 mm, and DOC export was 4.2 and 11.3 gC m^− 2 in the dry and wet year respectively. Analysis of the hydrographs showed that in both years most runoff and DOC export was generated during peak flow events, with low baseflow in the intermediate periods. Runoff response to rain events was strongest under a high water table in the spring and autumn, with a summer runoff minimum. The effect of drought on runoff occurred only after mid June when the effects of snowmelt had dissipated. Snowmelt therefore dominated DOC export in the dry year (61%) but contributed much less (29%) in the wet year. The relationship between runoff and the water table was highly similar in both years. Any variation that was observed herein was potentially related to a superficial subsidence of the peat surface under drier summer conditions and to raised spring water levels, causing restricted drainage due to flooding. The observed variation of DOC concentrations in both years generally had a limited impact on DOC export. During peak runoff events, concentrations dropped proportionally to the magnitude of the events. This decrease in available DOC is argued to be caused by a dominance of leaching over supply and production and by the specific hydraulic characteristics of the peatland surface. DOC concentrations were some 25% higher under drought conditions. These elevated concentrations were dispelled by spring snowmelt of the next year, demonstrating that a winter snowpack in boreal regions may be instrumental in limiting the longer term hydrological and biochemical effects of a drought.     

A dual-band direct-conversion RF front-end for WiMedia UWB receiver

This paper describes a direct-conversion RF front-end designed for a dual-band WiMedia UWB receiver. The front-end operates in band group BG1 and BG3 frequencies. It includes multi-stage LNAs, down-conversion mixers, a polyphase filter for quadrature local oscillator (LO) signal generation, and LO buffers. The UWB receiver is targeted for a mobile handset, where several other radios can be simultaneously on. Therefore, special attention was paid on minimizing the interference from different wireless systems. The front-end achieves approximately 26-dB gain and 4.9–5.6-dB noise figure (NF) across three sub-bands of BG1. In BG3 mode it obtains 23–26-dB gain and 6.9–7.7-dB NF. The front-end consumes 48.1 and 42.7 mA from a 1.2-V supply voltage in BG1 and BG3 operation modes, respectively. The chip was implemented in a 0.13-μm CMOS.     

Carbon nanotubes as electrodes for dielectrophoresis of DNA

Dielectrophoresis can potentially be used as an efficient trapping tool in the fabrication of molecular devices. For nanoscale objects, however, the Brownian motion poses a challenge. We show that the use of carbon nanotube electrodes makes it possible to apply relatively low trapping voltages and still achieve high enough field gradients for trapping nanoscale objects, e.g., single molecules. We compare the efficiency and other characteristics of dielectrophoresis between carbon nanotube electrodes and lithographically fabricated metallic electrodes, in the case of trapping nanoscale DNA molecules. The results are analyzed using finite element method simulations and reveal information about the frequency-dependent polarizability of DNA.     

Electromagnetic and mechanical design aspects of a high-speed solid-rotor induction machine with no separate copper electric circuit in the megawatt range

The most crucial electro-magnetic and mechanical design aspects of an integrated electrical-motor–gas-compressor system in high speed and high power operation are presented. The electric motor type considered is a solid-rotor induction motor with properties of which are particularly well suited in high-speed operation. The effect of the electro-magnetic material properties of the solid rotor core material on the performance of the machine is discussed. Guidelines to improve the performance of the solid-rotor induction motor are given. Thermal design aspects of a solid-rotor induction motor are presented. The mechanical properties of a solid rotor are discussed. Bearing arrangements as well as the rotor dynamics of an integrated system are presented.     

High-performance real-time quantitative RT-PCR using lanthanide probes and a dual-temperature hybridization assay

We report a time-resolved fluorescence-based, homogeneous approach for multiplex, real-time or end-point detection of PCR products. Signal generation consists of PCR associated digestion of a 5'-labeled oligonucleotide probe, rapid cooling of the reaction mixture, and hybridization of undigested probe oligonucleotides with a complementary, shorter probe that incorporates a quencher at its 3' end. The signal coming from intact fluorescent probe molecules is, thus, quenched. The fluorophores we have used are environmentally sensitive lanthanide chelates. Their signals can be measured in a time-resolved manner that eliminates most of the unspecific fluorescent background. Signal-to-noise ratios are further enhanced by the environmental sensitivity of these chelates; they exhibit a higher fluorescence intensity when free in solution than when coupled to intact probe molecules. Because of the minimal background fluorescence, the signal-to-noise ratios are higher and threshold cycles are lower than those obtained using conventional TaqMan probes. The multiplexing capacity of the assay chemistry is demonstrated through simultaneous amplification and detection of prostate specific antigen (PSA) cDNA and an internal standard mRNA (mmPSA) using probes labeled with terbium and europium. The applicability of the assay chemistry to routine clinical diagnostics is demonstrated through absolute quantification of PSA mRNA in peripheral blood.     

Functional inactivation of the conserved Sem1p in yeast by intrabodies

Intrabody technology was applied to characterize the function and intracellular localization of a highly conserved Saccharomyces cerevisiae Sem1 protein. DSS1, the mammalian homologue of Sem1p, is functionally conserved between yeast and mammalian cells, and in mammalian cells physically interacts with the strong tumour supressor BRCA2. Yeast and the generated intrabodies are thus expected to offer a useful system for studies on Sem1p/DSS1 function. Sem1p-specific antibody isolated from a phage display library was expressed intracellularily and targeted to either the cytosol or the nucleus of yeast cells. Analysis of the applicability of different antibody fragments as intrabodies showed that the Fab intrabody was expressed most efficiently. Expression of nuclear-targeted anti-Sem1p Fab intrabodies inhibited the growth of the sigma1278b yeast strain in a manner similar to deletion of the SEM1 gene. This indicates that the Fab intrabodies interact in vivo with Sem1p and result in inactivation of Sem1p. Localization of the Fab intrabody with or without the nuclear localization signal to the nucleus in Sem1p-dependent manner suggests that Sem1p mediates the nuclear transport of the intrabody without any targeting signal. Our results suggest that Sem1p function in yeast cells is in part manifested in the nucleus.     

Embryo cryopreservation and transfer in Mustelidae: Approaches to ex situ conservation of the endangered European mink

Successful cryopreservation of Mustelidae embryos was proved by development of frozen–thawed farmed European polecat (model species) embryos both in vitro and in vivo. Intra- and interspecies embryo transfer was performed between the endangered European mink (Mustela lutreola) and related Mustelidae species: European polecats (Mustela putorius) and domestic ferrets (Mustela putorius furo). Intraspecies embryo transfer was successful if more than two embryos were transferred. However, straightforward interspecies embryo transfers between different Mustelidae species have not resulted in term kits. Nonetheless, this paper demonstrates the possibility to overcome interspecies pregnancy failure by using European polecat/European mink hybrid females (called honoriks or nohoriks) as recipients for European mink embryos. Integrated package of reproductive technologies, i.e. production of polecat/mink hybrids, cryopreservation and subsequent transfer of European mink embryos into uterine horns of hybrid females is recommended as an option for ex situ conservation of the European mink.     

Bioavailability of sediment-associated PCDD/Fs and PCDEs: relative importance of contaminant and sediment characteristics and biological factors

Factors that determine accumulation of sediment-associated polychlorinated dibenzo-p-dioxins and furans and polychlorinated diphenyl ethers into semipermeable membrane devices (SPMDs) and benthic oligochaete worms (Lumbriculus variegatus) were examined. These factors included both physical-chemical and structural characteristics of the contaminants (water solubility, lipophilicity, dipole moment, molecular size, and conformation) and sediment characteristics (organic carbon content, particle size, aromaticity, and polarity of organic carbon). The results of partial least squares regression analysis indicated that lipophilicity alone is not a sufficient predictor for contaminant bioaccumulation potential, even though it is a significant contributor. It was shown that contaminant molecular size and conformation (specifically planarity/nonplanarity) as well as sediment characteristics also have a significant role. The studied factors contributed up to 63-88% of the variation in accumulation data for SPMDs and 50-65% for oligochaetes. Comparison of (bio)accumulation factors (BAF28d for oligochaetes and AF28d for SPMDs) revealed that accumulation of contaminants in oligochaetes is largely influenced by biological factors (e.g., feeding habits), while the physical-chemical nature of the process is emphasized for SPMDs.     

Density estimation with stagewise optimization of the empirical risk

We consider multivariate density estimation with identically distributed observations. We study a density estimator which is a convex combination of functions in a dictionary and the convex combination is chosen by minimizing the L ₂ empirical risk in a stagewise manner. We derive the convergence rates of the estimator when the estimated density belongs to the L ₂ closure of the convex hull of a class of functions which satisfies entropy conditions. The L ₂ closure of a convex hull is a large non-parametric class but under suitable entropy conditions the convergence rates of the estimator do not depend on the dimension, and density estimation is feasible also in high dimensional cases. The variance of the estimator does not increase when the number of components of the estimator increases. Instead, we control the bias-variance trade-off by the choice of the dictionary from which the components are chosen. Editor: Nicolo Cesa-Bianchi     

Buddy tracking — efficient proximity detection among mobile friends

Global positioning systems (GPS) and mobile phone networks make it possible to track individual users with an increasing accuracy. It is natural to ask whether this information can be used to maintain social networks. In such a network each user wishes to be informed whenever one of a list of other users, called the user’s friends, appears in the user’s vicinity. In contrast to more traditional positioning based algorithms, the computation here depends not only on the user’s own position on a static map, but also on the dynamic position of the user’s friends. Hence it requires both communication and computation resources. The computation can be carried out either between the individual users in a peer-to-peer fashion or by centralized servers where computation and data can be collected at one central location. In the peer-to-peer model, a novel algorithm for minimizing the number of location update messages between pairs of friends is presented. We also present an efficient algorithm for the centralized model, based on region hierarchy and quadtrees. The paper provides an analysis of the two algorithms, compares them with a naive approach, and evaluates them on user motions generated by the IBM City Simulator system.