The Use of Distinctive Monoclonal Antibodies in FMD VLP- and P1-Based Blocking ELISA for the Seromonitoring of Vaccinated Swine

The serum neutralization (SN) test has been regarded as the “gold standard” for seroconversion following foot-and-mouth disease virus (FMDV) vaccination, although a high-level biosafety laboratory is necessary. ELISA is one alternative, and its format is constantly being improved. For instance, standard polyclonal antisera have been replaced by monoclonal antibodies (MAbs) for catching and detecting antibodies, and inactive viruses have been replaced by virus-like particles (VLPs). To the best of current knowledge, however, no researchers have evaluated the performances of different MAbs as tracers. In previous studies, we successfully identified site 1 and site 2 MAbs Q10E and P11A. In this study, following the established screening platform, the VLPs of putative escape mutants from sites 1 to 5 were expressed and used to demonstrate that S11B is a site 3 MAb. Additionally, the vulnerability of VLPs prompted us to assess another diagnostic antigen: unprocessed polyprotein P1. Therefore, we established and evaluated the performance of blocking ELISA (bELISA) systems based on VLPs and P1, pairing them with Q10E, P11A, S11B, and the non-neutralizing TSG MAb as tracers. The results indicated that the VLP paired with S11B demonstrated the highest correlation with the SN titers (R² = 0.8071, n = 63). Excluding weakly positive serum samples (SN = 16–32, n = 14), the sensitivity and specificity were 95.65% and 96.15% (kappa = 0.92), respectively. Additionally, the P1 pairing with Q10E also demonstrated a high correlation (R² = 0.768). We also discovered that these four antibodies had steric effects on one another to varying degrees, despite recognizing distinct antigenic sites. This finding indicated that MAbs as tracers could not accurately detect specific antibodies, possibly because MAbs are bulky compared to a protomeric unit. However, our results still provide convincing support for the application of two pairs of bELISA systems: VLP:S11B-HRP and P1:Q10E-HRP.     

分布式实时多媒体数据库的研究与应用

讨论了多媒体数据库的结构 ,对多媒体数据库的实时传输与分布式特性问题分别进行了研究 ,并具体给出了 1个实际的应用实例 .     

循环流化床锅炉点火系统的数值模拟分析

循环流化床(CFB)锅炉点火系统主要以风道燃烧器为主。风道燃烧器在实际运行时,燃油着火和燃尽性能不是很理想,会出现火焰贴壁、壁面结焦等现象,因此对CFB锅炉点火系统进行数值模拟研究。针对内蒙古某电厂CFB锅炉风道燃烧器实际燃烧工况,选用非结构化有限元体积网格模型进行数值模拟。通过对点火系统的数值模拟和实测结果比较分析,验证了针对内蒙古某电厂CFB锅炉点火系统进行理论模拟所设置的物理模型和边界条件是符合实际的,理论模拟和实测结果的相对误差在10%范围内,结果较为理想。说明利用FLUENT软件对点火系统内温度场及压力场的情况进行模拟分析是可行的,对同类电厂具有一定的参考价值。     

数字图像传感器OV6630应用系统中的抖动问题及解决办法

本文介绍了以图像传感器OV6630、CPLD和C8051F单片机等为核心的图像采集系统,通过实验验证了在CPLD与C8051F单片机接口间存在的信号抖动问题.针对这一问题,根据微分电路原理.采用Verilog-HDL语言设计了去抖动模块,解决了图像采集系统的图像失真问题.     

配电自动化系统分析及改进方案

配电网是电力网络中直接面向电力用户的一个环节,是检测供电可靠性的重要部分。配电自动化的广泛应用,为调度运行控制能力、故障处理能力和配网检修能力的提高奠定了基础,也对社会保电活动等提供可信的技术手段和技术措施,极大减少停电次数和停电时间。本文结合西咸新区供电公司成立后的配电网规划,对配电网的发展现状、存在的问题进行分析,并从主站信息和馈电线路方面提出了切实有效的改进方案。     

A new optimal digital halftoning technique based on the discrete cosine transform

Digital halftoning is a technique to display a gray-level image with a bilevel device. Conventionally, most halftoning techniques are done in the spatial domain. A new halftoning technique based on the discrete cosine transform is proposed. The method chooses an optimal bilevel image to display the original gray-level image and minimize the weighted mean square error based on the discrete cosine transform domain. The simulation results indicate that our algorithm can produce very good halftoned images without false contours.     

A 0.7-2-GHz self-calibrated multiphase delay-locked loop

A 0.7-2-GHz precise multiphase delay-locked loop (DLL) using a digital calibration circuit is presented. Incorporating with the proposed digital calibration circuit, the mismatch-induced timing error among multiphase clocks in the proposed DLL can be self-calibrated. When the calibration procedure is finished, the digital calibration circuit can be turned off automatically to save power dissipations and reduce noise generations. A start controlled circuit is proposed to enlarge the operating frequency range of the DLL. Both the start-controlled circuit and the calibration circuit require an external reset signal to ensure the correctness of the calibration after temperature,operating frequency, and power supply voltage are settled. This DLL with the digital calibration circuit has been fabricated in a 0.18-/spl mu/m CMOS process. The measured results show the DLL exhibits a lock range of 0.7-2 GHz while the peak-to-peak jitter and rms jitter is 18.9ps and 2.5 ps at 2 GHz, respectively. When the calibration procedure is completed and the DLL operates at 1 GHz, the maximum mismatch-induced timing error among multiphase clocks is reduced from 20.4 ps (7.34 degree) to 3.5 ps (1.26 degree).     

Field Emission and Cathodoluminescence of ZnS Hexagonal Pyramids of Zinc Blende Structured Single Crystals

Single‐crystal hexagonal pyramids of zinc blende ZnS are fabricated by facile thermal evaporation in an ammonia atmosphere at 1150 °C. It is found that ZnS pyramids grow along the [111] crystal axis and possess a sharp tip with a diameter of ～10 nm and a micrometer‐sized base. The structural model and growth mechanism are proposed based on crystallographic characteristics. This unique ZnS pyramid structure exhibits a low turn‐on field (2.81 V µm^?1), a high field‐enhancement factor (over 3000), a large field‐emission current density (20 mA cm^?2), and good stability with very small fluctuation (0.9%). These superior field‐emission properties are clearly attributed to the pyramid morphology, with micrometer‐sized bases and nanotips, and high crystallinity. Moreover, a stable UV emission of 337 nm at room temperature is observed and can be ascribed to the band emission of the zinc blende phase. These results suggest that the ZnS hexagonal pyramids can be expected to find promising applications as field emitters and optoelectronic devices.     

Rational Design of Charge‐Neutral,Near‐Infrared‐Emitting Osmium(II) Complexes and OLED Fabrication

A new series of charge neutral Os(II) isoquinolyl triazolate complexes ( 1 – 4 ) with both trans and cis arrangement of phosphine donors are synthesized, and their structural, electrochemical and photophysical properties are established. In sharp contrast to the cis‐arranged complexes 2 – 4 , the trans derivative 1 , which shows a planar arrangement of chromophoric N‐substituted chelates, offers the most effective extended π‐delocalization and hence the lowest excited state energy gap. These complexes exhibit phosphorescence with peak wavelengths ranging from 692–805 nm in degassed CH₂Cl₂ at room temperature. Near‐infrared (NIR)‐emitting electroluminescent devices employing 6 wt % of 1 (or 4 ) doped in Alq₃ host material are successfully fabricated. The devices incorporating 1 as NIR phosphor exhibit fairly intense emission with a peak wavelength at 814 nm. Forward radiant emittance reaches as high as 65.02 µW cm^?2, and a peak EQE of ～1.5% with devices employing Alq₃, TPBi and/or TAZ as electron‐transporting/exciton‐blocking layers. Upon switching to phosphor 4 , the electroluminescence blue shifts to 718 nm, while the maximum EQE and radiance increase to 2.7% and 93.26 (μW cm^?2) respectively. Their performances are optimized upon using TAZ as the electron transporting and exciton‐blocking material. The OLEDs characterized represent the only NIR‐emitting devices fabricated using charge‐neutral and volatile Os(II) phosphors via thermal vacuum deposition.     

Discrimination of breast cancer by measuring prostate-specific antigen levels in women's serum

Prostate-specific antigen (PSA) has been reported to be a potential biomarker of breast cancer. Serum PSA of normal women is around 1 pg/mL, which is usually undetectable by current assay methods; thus an ultrasensitive measurement of PSA expression in women's serum is necessary to distinguish normal from malignant breast diseases. To enhance the sensitivity of conventional immunoassay technology for the detection of PSA in sera, we adopted a localized surface plasmon coupled fluorescence fiber-optic biosensor, which combines a sandwich immunoassay with the localized surface plasmon technique. The concentration of total PSA (t-PSA) (from 0.1 to 1000 pg/mL) in phosphate-buffered saline solution and the normalized fluorescence signal exhibit a linear relationship where the correlation coefficient is 0.9574. In addition, the concentration of additional t-PSA in 10-fold-diluted healthly women's serum across a similar range was measured. The correlation coefficient for this measurement is 0.9142. In clinical serum samples, moreover, the experimental results of t-PSA detection show that both the mean value and median of normalized fluorescence signals in the breast cancer group (155.2 and 145.7, respectively) are higher than those in the noncancer group (46.6 and 37.1, respectively). We also examined the receiver operating characteristic curve for t-PSA, and the area under the curve (AUC) is estimated to be 0.9063, the AUC being used to measure the performance of a test to correctly identify diseased and nondiseased subjects.