多重PCR-液相芯片技术检测13个品系转基因玉米

为建立多重PCR-液相芯片检测13个品系的转基因玉米的方法,针对BT11,BT176,Mon810,Mon863,T25,GA21,NK603,TC1507,Mon89034,Mir162,Mon88017,MIR604和BVLA430101这13种玉米品系的侧翼序列,设计合成了品系特异性引物/探针。在生物素标记的PCR扩增产物与固定在荧光编码微球上的品系特异探针杂交后,对微球进行流式检测。试验结果显示,13种转基因玉米的引物和探针具有较高的特异性,引物/探针相互之间无交叉扩增和非特异杂交,大部分转基因玉米品系的检测灵敏度达到0.1%水平,符合欧盟和其他国家有关转基因产品标识的要求。本方法的检测效率和准确性均高于传统方法,可作为进出口转基因产品和国内转基因检测的有效方法。     

花生功能成分及其综合利用

介绍了世界和我国的花生种植和生产情况,综述了花生的功能成分及其特性.对花生油、花生蛋白、花生种皮和花生壳的开发现状进行了较全面的总结,对花生中的磷脂、维生素E、植物甾醇、蛋白质、维生素K、白藜芦醇和粗纤维等功能成分在食品、医药、饲料和化工等行业中的应用前景进行了较详尽的讨论,可为合理有效地综合利用花生资源提供有价值的参考.     

Aggregation kinetics of alginate-coated hematite nanoparticles in monovalent and divalent electrolytes

The early stage aggregation kinetics of bare and alginate-coated hematite nanoparticles are acquired through time-resolved dynamic light scattering (DLS). Varying concentrations of monovalent (NaCl) and divalent (MgCl2 and CaCl2) electrolytes are employed to induce aggregation. In the presence of NaCl and MgCl2, the alginate-coated hematite nanoparticles undergo aggregation through electrostatic destabilization as described by the classic Derjaguin-Landau-Verwey-Overbeek (DLVO) theory. This is ascertained through examination of the favorable and unfavorable regimes of the stability curves depicting the attachment efficiency as a function of salt concentration. Additional evidence may be found in the aggregation kinetics of alginate-coated particles, which, under favorable aggregation conditions, are reasonably close to that of bare hematite nanoparticles. However, in the presence of CaCl2, the aggregate growth rate of alginate-coated hematite nanoparticles is much higher than that which conventional diffusive aggregation predicts. Dispersed hematite primary particles and lower-order aggregates enmeshed within extended alginate gel networks were observed under transmission electron microscope (TEM). The proposed mechanism for enhanced aggregation suggests an apparent increase in the collision radii of alginate-coated hematite nanoparticles through alginate gel network formation from the particle surface. Additionally, cross-linking between unadsorbed (suspended) alginate macromolecules may form bridges between hematite-alginate gel clusters. It is further established that the presence of background electrolyte NaCl in solution is detrimental to the calcium-induced enhanced aggregation.     

精炼渣改性后的成块和熔化性能测试

将作为固体废弃物的精炼渣,经过适当地调整成分,造块后返回转炉炼钢冶炼过程,可以实现精炼渣少排放或零排放.本试验通过正交试验对精炼渣和改性后的合成渣的抗压性能、熔化性能进行了测试.试验结果表明,合成渣的成块性能和熔化性能可以满足转炉炼钢的要求.     

利用VC++/C编程实现Windows 与Linux的网络通信

结合VC++和C语言在某火灾监控系统中的实际应用,讨论了在嵌入式操作系统Linux和Windows间实现网络通信的关键技术,并给出了通信软件的基本原理和开发步骤.     

基于PIC16F913的手持编程器的系统设计与实现

介绍基于单片机PIC16F913的手持编程器的系统设计与实现。广泛应用于液晶控制、驱动和显示的芯片PIC16F913为设计小体积、低功耗、高性能的手持编程器提供了极大的方便。     

Influence of Hot Rolling on Cube Texture of Ni Substrate for Coated Conductor

Pure Ni and Ni alloy tapes with sharp cube texture and low-angle grain boundaries prepared by thermomechanical process were extensively used as substrates for coated conductor. The thermomechanical process usually includes hot forging and cold rolling. In this study, a hot-rolling process between hot forging and cold rolling was induced. The influence of hot rolling on the cube texture of pure Ni was discussed. Sharp cube texture on pure Ni was obtained by suitable hot rolling, cold rolling, and recrystallization treatment. This deformation texture of tape was studied using orientation distribution function （ODF）. Orientation mapping, content of the cube texture, and grain boundary distribution were performed using an EBSD system mounted on LEO-1450 SEM. The results show that the substrates that are hot rolled have a sharp cube texture and low-angle grain boundaries.     

高精度超声波测距系统

介绍了利用超声波传感器实现无接触式空气测距的原理和硬件组成,并充分考虑到测量环境温度对超声波传递速度的影响,通过温度补偿的方法对传递速度予以校正,而且可以马上检测声波在现场的传播速度并被采纳,因此具有非常高的测量精度,能实时地将测量数据上传给PC机,具有硬件结构简单、工作可靠、测量误差小等特点,现该产品已经被上海造纸机械电控技术研究所用于造纸设备复卷机的旋转辊位移检测上,实践证明达到了各项指标的要求。     

矿山水害分类与断层防水煤柱存在问题分析

根据涌水水源和渗透通道的匹配关系,地下矿山水害有透地表水和断层透水等7种可能的透水形式,可归纳为顶部透水、同层位透水和底板突水等3种透水类型。在采掘工作空间和涌水水源之间留设一定宽度的隔水煤岩柱来防止水害的出现和蔓延,是目前国内外最常用的防治水措施之一。结合断层防水煤柱的推导,对现阶段断层防水煤柱存在的主要问题进行了全面分析,指出隔水煤柱的设计应充分考虑煤体软化、压力水与煤体的相互作用以及矿山压力作用下的煤柱分区等相关问题。     

新型CeO2-Fe2O3联合催化剂用于催化CO还原NO

采用3种不同的方法合成新型的以Fe₂O₃颗粒为载体的Ce-Fe复合氧化物催化剂,探究不同制备方式的复合氧化物催化剂的催化CO还原NO性能,并通过X射线衍射(XRD)、拉曼光谱(Raman)、氢气-程序升温还原(H2-TPR)、X射线光电子能谱(XPS)表征来对催化剂进行探究。结果证明Ce-Fe复合氧化物具有较高的催化活性,硝酸水热法Ce-Fe复合氧化物在900℃时脱硝率可达到99%以上。XRD中显示硝酸水热样品中CeO₂峰向高角度偏移,并且晶格常数变小。Raman结果说明硝酸水热样品CeO₂的振动峰向左大幅度偏移,结合XRD说明硝酸水热法可以形成Ce-O-Fe固溶体。H2-TPR说明氧化还原性:硝酸水热法>硝酸浸渍法>普通浸渍法。XPS结果说明硝酸水热法形成的Ce-O-Fe固溶体可以促进Fe³⁺向Fe²⁺、晶格氧向吸附氧的转换,这体现出复合催化剂Ce、Fe之间的联合作用。在整个反应过程中Fe₂O₃作为载体可以提供大量晶格氧,Ce-O-Fe固溶体的存在决定了复合催化剂的高催化活性。