Proteomics of plaques and novel sources of potential biomarkers for atherosclerosis

Cardiovascular disease (CVD) is the leading cause of death and loss of productive life years in the world. The underlying syndrome of CVD, atherosclerosis, is a complex disease process, which involves lipid metabolism, inflammation, innate and adaptive immunity, and many other pathophysiological aspects. Furthermore, CVD is influenced by genetic as well as environmental factors. Early detection of CVD and identification of patients at risk are crucial to reduce the burden of disease and to allow personalized treatment. As established risk factors fail to accurately predict which part of the population is likely to suffer from the disease, novel biomarkers are urgently needed. Proteomics can play a significant role in identifying these biomarkers. In this review, we describe the progress made in proteome profiling of the atherosclerotic plaque and several novel sources of potential biomarkers, including circulating cells and plasma extracellular vesicles. The importance of longitudinal biobanking in biomarker discovery is highlighted and exemplified by several plaque proteins identified in the biobank study Athero-Express. Finally, we discuss the PTMs of proteins that are involved in atherosclerosis, which may become one of the foci in the ongoing quest for biomarkers through proteomics of plaque and other matrices relevant to the progression of atherosclerosis.     

Remote flood monitoring system based on plastic optical fibres and wireless motes

This paper summarises an effort in the development of a remote flood monitoring system based on plastic optical fibre (POF) sensors and a wireless mote network. The wireless mote, comprising of a network of MICA2DOT™ units, was used as a platform to monitor and record the signal from the POF sensors and transmit this information to a base station wirelessly. A prototype of the integrated wireless POF sensor unit has been constructed, rendering it possible to deploy the autonomous unit remotely at multiple monitoring points as required. A flood monitoring simulation was carried out in a 24 m × 10 m × 0.9 m wave basin where four of these wireless optical fibre mote sensors were used to detect the rising water level in the basin. The novelty of the work lies in the successful integration of the wireless platform to a POF-based liquid level sensor and the subsequent demonstration of the prototype of the system for the purposes of flood monitoring applications.The sensing principle of the POF sensor developed here is well-known and is based on the loss of total internal reflection of the optical signal as the sensor probe comes in contact with the liquid. Compared to optical fibre-based sensors reported previously in the literature, the probe profile used in this study differs in terms of its simplicity in design, while exhibiting an excellent signal intensity loss ratio without the need for additional attachments to the probe such as optical prisms. The tests carried out showed that the POF sensor is capable of detecting a variety of fluids. Exhibiting good signal stability, the sensor also detects the liquid level reliably when the liquid rises or falls to the predetermined level. The responsiveness of the optical fibre sensor was evaluated by simulating different rates at which the liquid rises by immersing the sensor tip into the liquid and vice-versa at various speeds ranging from 1 mm/min to 500 mm/min.     

Laser-Printed Plasmonic Metasurface Supporting Bound States in the Continuum Enhances and Shapes Infrared Spontaneous Emission of Coupled HgTe Quantum Dots

In order to advance the development of quantum emitter-based devices, it is essential to enhance light-matter interactions through coupling between semiconductor quantum dots with high quality factor resonators. Here, efficient tuning of the emission properties of HgTe quantum dots in the infrared spectral region is demonstrated by coupling them to a plasmonic metasurface that supports bound states in the continuum. The plasmonic metasurface, composed of an array of gold nanobumps, is fabricated using single-step direct laser printing, opening up new opportunities for creating exclusive 3D plasmonic nanostructures and advanced photonic devices in the infrared region. A 12-fold enhancement of the photoluminescence in the 900–1700 nm range is observed under optimal coupling conditions. By tuning the geometry of the plasmonic arrays, controllable shaping of the emission spectra is achieved, selectively enhancing specific wavelength ranges across the emission spectrum. The observed enhancement and shaping of the emission are attributed to the Purcell effect, as corroborated by systematic measurements of radiative lifetimes and optical simulations based on the numerical solution of Maxwell's equations. Moreover, coupling of the HgTe photoluminescence to high quality factor modes of the metasurface improves emission directivity, concentrating output within an ≈20° angle.     

3D Printing Thermally Stable High-Performance Polymers Based on a Dual Curing Mechanism

High-performance polymers are an important class of materials that are used in challenging conditions, such as in aerospace applications. Until now, 3D printing based on stereolithography processes can not be performed due to a lack of suitable materials. There is report on new materials and printing compositions that enable 3D printing of objects having extremely high thermal resistance, with Tg of 283 °C and excellent mechanical properties. The printing is performed by a low-cost Digital Light Processing printer, and the formulation is based on a dual-cure mechanism, photo, and thermal process. The main components are a molecule that has both epoxy and acrylate groups, alkylated melamine that enables a high degree of crosslinking, and a soluble precursor of silica. The resulting objects are made of hybrid materials, in which the silicon is present in the polymeric backbone and partly as silica enforcement particles.     

Hybrid Simulation of Miscible Mixing with Viscous Fingering

By modeling mass transfer phenomena, we simulate solids and liquids dissolving or changing to other substances. We also deal with the very small‐scale phenomena that occur when a fluid spreads out at the interface of another fluid. We model the pressure at the interfaces between fluids with Darcy's Law and represent the viscous fingering phenomenon in which a fluid interface spreads out with a fractal‐like shape. We use hybrid grid‐based simulation and smoothed particle hydrodynamics (SPH) to simulate intermolecular diffusion and attraction using particles at a computable scale. We have produced animations showing fluids mixing and objects dissolving.     

The Kemeny Constant for Finite Homogeneous Ergodic Markov Chains

A quantity known as the Kemeny constant, which is used to measure the expected number of links that a surfer on the World Wide Web, located on a random web page, needs to follow before reaching his/her desired location, coincides with the more well known notion of the expected time to mixing, i.e., to reaching stationarity of an ergodic Markov chain. In this paper we present a new formula for the Kemeny constant and we develop several perturbation results for the constant, including conditions under which it is a convex function. Finally, for chains whose transition matrix has a certain directed graph structure we show that the Kemeny constant is dependent only on the common length of the cycles and the total number of vertices and not on the specific transition probabilities of the chain.     

Finding Pathway Structures in Protein Interaction Networks

The increased availability of data describing biological interactions provides important clues on how complex chains of genes and proteins interact with each other. Most previous approaches either restrict their attention to analyzing simple substructures such as paths or trees in these graphs, or use heuristics that do not provide performance guarantees when general substructures are analyzed. We investigate a formulation to model pathway structures directly and give a probabilistic algorithm to find an optimal path structure in time and space, where n and m are respectively the number of vertices and the number of edges in the given network, k is the number of vertices in the path structure, and t is the maximum number of vertices (i.e., "width") at each level of the structure. Even for the case t = 1 which corresponds to finding simple paths of length k, our time complexity is a significant improvement over previous probabilistic approaches. To allow for the analysis of multiple pathway structures, we further consider a variant of the algorithm that provides probabilistic guarantees for the top suboptimal path structures with a slight increase in time and space. We show that our algorithm can identify pathway structures with high sensitivity by applying it to protein interaction networks in the DIP database.     

Multi-region Trefftz boundary element method for fracture analysis in plane piezoelectricity

This paper presents a multi-region Trefftz boundary element method for fracture analysis in plane piezoelectricity. To model the sub-region that contains the crack, a special set of Trefftz functions that satisfy the traction-free and charge-free conditions along the crack faces are constructed. To model the remaining sub-regions, the basic set of Trefftz functions co-derived previously by the authors are employed. With the two sets of Trefftz functions, the multi-region Trefftz boundary element method is formulated by point collocation. The special set of Trefftz functions exempts all the boundary treatment of the crack faces and enables the direct determination of the electromechanical intensity factors. Numerical examples are presented to illustrate the efficacy of the formulation.     

Thermal and dielectric properties of ZnO-B2O3-MO3glasses (M = W, Mo)

Glasses in the ZnO-B₂O₃-MO₃(M = W, Mo) ternary were examined as potential replacements to PbO-B₂O₃-SiO₂-ZnO glass frits with the low firing temperature (500–600^∘C) for the dielectric layer of a plasma display panels (PDPs). Glasses were melted in air at 950–1150^∘C in a narrow region of the ternary using standard reagent grade materials. The glasses were evaluated for glass transition temperature (T _g ), softening temperature (T _d ), the coefficient of thermal expansion (CTE), dielectric constant (ε _r ), and optical property. The glass transition temperature of the glasses varied between 470 and 560^∘C. The coefficient of thermal expansion and the dielectric constant of the glasses were in the range of 5–8 × 10^{− 6}/^∘C and 8–10, respectively. The addition of MO₃to ZnO-B₂O₃binary could induce the expansion of glass forming region, the reduction of T _g and the increase in the CTE and the dielectric constant of the glasses. Also, the effect of the addition of MO₃to ZnO-B₂O₃binary on the transmittance in the visible-light region (350–700 nm) was investigated.