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91.
92.
Zymomonas mobilis is undoubtedly one of the most unique bacterium within the microbial world. Known since 1912 under the names Termobacterium mobilis, Pseudomonas linderi, and Zymomonas mobilis, reviews on its uniqueness have been published in 1977 and 1988. The bacterium Zymomonas mobilis not only exhibits an extraordinarily uniqueness in its biochemistry, but also in its growth behavior, energy production, and response to culture conditions, as well as cultivation techniques used. This uniqueness caused great interest in the scientific, biotechnological, and industrial worlds. Its ability to couple and uncouple energy production in favor of product formation, to respond to physical and chemical environment manipulation, as well as its restricted product formation, makes it an ideal microorganism for microbial process development. This review explores the advances made since 1987, together with new developments in the pure scientific and applied commercial areas. 相似文献
93.
An engineering model for short-channel MOS devices 总被引:1,自引:0,他引:1
An engineering model for short-channel MOS devices which includes the effect of carrier drift velocity saturation is described. Based on a piecewise carrier drift velocity model, simplified expressions for the DC drain current I D, the small signal transconductance g m and the output conductance g ds in the saturation region are derived. For a given gate voltage, the expressions depend only on the threshold voltage V T and the dimensions of the device, whose desired values are normally known 相似文献
94.
Chew P. Yeap C.B. Cham J. Goh A. Rossi N. Singh R. Ong K. Toh H.T. 《Semiconductor Manufacturing, IEEE Transactions on》2007,20(4):482-487
In mass manufacturing, the quality of aluminum interconnect films could be compromised due to various reasons. Such a compromise could result in electrical shorts and hence failure to yield at the end of the line or, worse, could result in reliability failure such as electromigration if the film morphology or linewidth is affected. As a result of such potential failures, wafers with these types of problems are usually scrapped at the point of detection inline. Scrapping wafers in the line or, worse, at the end of the line, results in financial loss as well as potential delivery problems to customers if there are no backup wafers in the vicinity of the affected step to replace the scrapped wafers. Missing timely delivery to a customer may impact not only the immediate customer but also the customer's customers who may be dependent upon uninterrupted and timely supply in their lean supply chain management system. This paper describes a study done that demonstrated the successful recovery of such problematic wafers along with verification by inline and end of line testing, including device functionality and reliability verification. This feasibility opens the door to avoid potentially huge losses and undesirable delivery impact to multiple stakeholders in the supply chain where time to market is important. 相似文献
95.
96.
VM Gitlits JW Sentry ML Matthew AI Smith BH Toh 《Canadian Metallurgical Quarterly》1997,92(3):362-368
Although the pathology of discoid lupus erythematosus is well documented the causative agents are not known. Here, we report the identity of the target antigen of an autoantibody present in high titre in the serum of a patient with discoid lupus erythematosus. We have demonstrated that the antigen is enolase; first, because it has properties consistent with this glycolytic enzyme (47,000 MW, cytosolic localization and ubiquitous tissue distribution). Secondly, limited amino acid sequence determination after trypsin digestion shows identity with alpha-enolase. Finally, the autoimmune serum immunoblots rabbit and yeast enolase and predominantly one isoelectric form of enolase (PI approximately 6.1). These results indicate that the reactive autoepitopes are highly conserved from man to yeast. The results also suggest that the autoantibodies are most reactive to the alpha-isoform of enolase, although it is possible that they may also be reactive with gamma-enolase, and have least reactivity to beta-enolase. The anti-enolase autoantibodies belong to the immunoglobulin G1 (IgG1) isotype. This is the first report of IgG1 autoantibodies to evolutionarily conserved autoepitopes of enolase in the serum of a patient with discoid lupus erythematosus. Previous reports of autoantibodies to enolase have suggested associations with autoimmune polyglandular syndrome type I and cancer-associated retinopathy. This report and an earlier report of what is likely to be enolase autoantibodies in two patients without systemic disease suggest that enolase autoantibodies have a broad association and are not restricted to any particular disease. 相似文献
97.
The photolyase-blue-light photoreceptor family is composed of cyclobutane pyrimidine dimer (CPD) photolyases, (6-4) photolyases, and blue-light photoreceptors. CPD photolyase and (6-4) photolyase are involved in photoreactivation for CPD and (6-4) photoproducts, respectively. CPD photolyase is classified into two subclasses, class I and II, based on amino acid sequence similarity. Blue-light photoreceptors are essential light detectors for the early development of plants. The amino acid sequence of the receptor is similar to those of the photolyases, although the receptor does not show the activity of photoreactivation. To investigate the functional divergence of the family, the amino acid sequences of the proteins were aligned. The alignment suggested that the recognition mechanisms of the cofactors and the substrate of class I CPD photolyases (class I photolyases) are different from those of class II CPD photolyases (class II photolyases). We reconstructed the phylogenetic trees based on the alignment by the NJ method and the ML method. The phylogenetic analysis suggested that the ancestral gene of the family had encoded CPD photolyase and that the gene duplication of the ancestral proteins had occurred at least eight times before the divergence between eubacteria and eukaryotes. 相似文献
98.
We present a modified method of shearography, known herein as multiple-image shearography, whereby the curvatures of an object can be measured directly from the resulting fringes. It employs an image-shearing camera that produces three sheared images simultaneously to interfere with each other in the image plane. When film is doubly exposed before and after an object is deformed, three sets of fringes are observed of which one set would depict the second-order derivatives of surface displacement.The theory of the multiple-image shearography technique and its application to curvature measurements in plate bending are presented. 相似文献
99.
A whole-field method of double-exposure speckle photography is employed to determine metal surface roughness by correlation between two speckle patterns. A movable rectangular aperture that is mounted before an image lens is shifted between the exposures, which results in a decrease in the contrast of the reconstructed Young's fringes with increasing roughness. The technique permits evaluation of the roughness of particular points on a surface as well as the average roughness of an entire surface. Four sets of random surfaces that were prepared by different machine-finishing processes and with roughnesses ranging from 0.6 to 13 μm have been tested. Different methods have been carried out to process the test data, and a practical method for the evaluation of surface roughness is proposed. 相似文献
100.
FT Mu JM Callaghan O Steele-Mortimer H Stenmark RG Parton PL Campbell J McCluskey JP Yeo EP Tock BH Toh 《Canadian Metallurgical Quarterly》1995,270(22):13503-13511
Early endosomes are cellular compartments receiving endocytosed material and sorting them for vesicular transport to late endosomes and lysosomes or for recycling to the plasma membrane. We have cloned a human cDNA encoding an evolutionarily conserved 180-kDa protein on early endosomes named EEA1 (Early Endosome Antigen1). EEA1 is associated with early endosomes since it co-localizes by immunofluorescence with the transferrin receptor and with Rab5 but not with Rab7. Immunoelectron microscopy shows that it is associated with tubulovesicular early endosomes containing internalized bovine serum albumin-gold. EEA1 is a hydrophilic peripheral membrane protein present in cytosol and membrane fractions. It partitions in the aqueous phase after Triton X-114 solubilization and is extracted from membranes by 0.3 M NaCl. It is a predominantly alpha-helical protein sharing 17-20% sequence identity with the myosins and contains a calmodulin-binding IQ motif. It is flanked by metal-binding, cysteine "finger" motifs. The COOH-terminal fingers, Cys-X2-Cys-X12-Cys-X2-Cys and Cys-X2-Cys-X16-Cys-X2-Cys, are present within a region that is strikingly homologous with Saccharomyces cerevisiae FAB1 protein required for endocytosis and with Caenorhabditis elegans ZK632. These fingers also show limited conservation with S. cerevisiae VAC1, Vps11, and Vps18p proteins implicated in vacuolar transport. We propose that EEA1 is required for vesicular transport of proteins through early endosomes and that its finger motifs are required for this activity. 相似文献