Replication of sub‐micron features using amorphous thermoplastics

A comprehensive experimental study was carried out to replicate sub‐micron features using the injection molding technique. For the experiments, five different plastic materials were selected according to their flow properties. The materials were polycarbonate (PC), styrene‐butadiene block copolymer (SBS), impact modified poly(methyl methacrylate), methyl methacrylate‐acrylonitrile‐butadiene‐styrene polymer (MABS), and cyclic olefin copolymer (COC). Nanofeatures down to 200‐nm line width and with aspect ratios (aspect ratio = depth/width) of 1:1 could be replicated. In all selected materials, the greatest differences between the materials emerged when the aspect ratio increased to 2:1. The most favorable results were obtained with the use of high flow polycarbonate as the molding material. The best replication results were achieved when melt and mold temperatures were higher than normal values.     

Recent advances in maneuver loads analysis

The conceptual design of an aircraft system requires a number of analysis cycles involving the study of various configurations for which aerodynamic and structural properties are not well defined. The aeroelastic stability and structural strength considerations are very important factors in the determination of the aerodynamic and the structural configurations. Therefore, this paper briefly reviews the specifications leading to the design loads criteria and the current analysis methods. Suggestions for research activities required in the development of a computational fluid dynamics (CFD) code and its application to predict the design loads are also included.     

Ion-implanted capacitively coupled silicon strip detectors with integrated polysilicon bias resistors processed on a 100 mm wafer

Double-sided silicon strip detectors with integrated coupling capacitors and polysilicon resistors have been processed on a 100 mm wafer. A detector with an active area of 19 × 19 mm² was connected to LSI readout electronics and tested. The strip pitch of the detector is 25 μm on the p-side and 50 μm on the n-side. The readout pitch is 50 μm on both sides. The number of readout strips is 774 and the total number of strips is 1161. On the p-side a signal-to-noise of 35 has been measured using a ⁹⁰Sr β-source. The n-side has been studied using a laser.     

Etiology of pneumonia and other common childhood infections requiring hospitalization and parenteral antimicrobial therapy. SE-TU Study Group

The etiology of acute lower respiratory tract infections (mostly pneumonia) in children is well characterized, but these are only some of the community-acquired infections warranting parenteral antimicrobial therapy. We prospectively evaluated all such infections among children aged 3 months to 15 years by use of blood cultures, examination of nasopharyngeal aspirates, and serologies for 15 viral, 7 bacterial, and 1 protozoal agent. Immunocompromised patients and those with urinary tract infection, meningitis, or osteoarticular infection were excluded. In all, 170 children were included. The pathogenic agent was identified in 62% of the cases. Bacteria were detected in 54%, and a pneumococcus was found in 59% of the cases identified. Viruses were found in 15% overall. Sole bacterial or viral infections were detected in 47.1% and 8.1%, respectively. Since thorough screening established the etiology in less than two-thirds of patients ill enough to be hospitalized and treated parenterally, better diagnostics are needed, especially to identify those who would truly benefit from antimicrobial therapy.     

Studies on steam condensation and particle diffusiophoresis in a heat exhanger tube

In this paper, studies on steam condensation and aerosol behaviour in a heat exchanger are presented. The heat exchanger is a model of one single tube of a Passive Containment Condenser (PCC) that is used in the European Simplified Boiling Water Reactor (ESBWR). A hot carrier gas containing nitrogen and steam with Ag and CsOH particles flows through the heat exchanger. The walls of the heat exchanger are cooled with a water jacket, thus causing steam condensation and diffusiophoretic particle deposition. The amount of condensed steam is measured, as well as the temperatures, particle mass concentrations and size distributions before and after the heat exchanger. The experiments are done with different proportions of steam and nitrogen in the carrier gas. Heat and mass transfer in the system are modelled with well known engineering correlations, producing results that agree nicely with the experimental results. The diffusiophoretic particle deposition velocity is shown to be proportional to the steam condensation rate, as expected.     

Current-Mode Temperature Compensated Continuous-Time CMOS Transconductance-C Filter

In this paper a low voltage low power current-mode continuous-timelow-pass filter is presented. The current-integrator is constructedof a multi-output linearized transconductance element drivenby a balanced transimpedance amplifier. A 1 MHz third order ellipticlow pass filter is designed and fabricated with the proposedcurrent-mode integrator. The measurements show capability ofoperation with down to a 3 V supply voltage. The measured currentconsumption is 850 µA at room temperature andthe dynamic range is 66 dB. The distortion of the filter is below50 dBc with peak signal levels up to twice the quiescent currentlevel and with the refined designs even lower distortion is expected.The filter uses a CMOS-current reference for temperature andprocess variation stabilization with a measured corner frequencytemperature coefficient of –100 ppm/K.     

Several cooperating binding sites mediate the interaction of a lysosomal enzyme with phosphotransferase

Lysosomal targeting of soluble lysosomal hydrolases is mediated by mannose 6-phosphate receptors, which recognize and bind mannose 6-phosphate residues in the oligosaccharide chains of proteins destined for delivery to lysosomes. This recognition marker is generated by the sequential action of two enzymes, the first of which, UDP-N-acetylglucosamine phosphotransferase, recognizes lysosomal enzymes on the basis of a structural determinant in their polypeptide chains. This recognition event is a key step in lysosomal targeting of soluble proteins, but the exact nature of the recognition determinant is not well understood. In this study we have characterized the phosphotransferase recognition signals of human lysosomal aspartylglucosaminidase (AGA) using transient expression of polypeptides carrying targeted amino acid substitutions. We found that three lysine residues and a tyrosine residing in three spatially distinct regions of the AGA polypeptide are necessary for phosphorylation of the oligosaccharides. Two of the lysines are especially important for the lysosomal targeting efficiency of AGA, which seems to be mostly dictated by the degree of phosphorylation of the alpha subunit oligosaccharide. On the basis of the results of this and previous studies we suggest a general model for recognition of lysosomal enzymes by the phosphotransferase.     

The mitochondrial toxin produced by Streptomyces griseus strains isolated from an indoor environment is valinomycin

Actinomycete isolates from indoor air and dust in water-damaged schools and children's day care centers were tested for toxicity by using boar spermatozoa as an indicator. Toxicity was detected in extracts of four strains which caused a loss of sperm motility, and the 50% effective concentrations (EC50) were 10 to 63 ng (dry weight) ml of extended boar semen-1. The four strains were identified as Streptomyces griseus strains by 16S ribosomal DNA and chemotaxonomic methods. The four S. griseus strains had similar effects on sperm cells, including loss of motility and swelling of mitochondria, but we observed no loss of plasma membrane integrity or depletion of cellular ATP. None of the effects was observed with sperm cells exposed to extracts of other indoor actinomycete isolates at concentrations of >/=5,000 to 72,000 ng ml-1. The toxin was purified from all four strains and was identified as a dodecadepsipeptide, and the fragmentation pattern obtained by tandem mass spectrometry was identical to that of valinomycin. Commercial valinomycin had effects in sperm cells that were identical to the effects of the four indoor isolates of S. griseus. The EC50 of purified toxin from the S. griseus strains were 1 to 3 ng ml of extended boar semen-1, and the EC50 of commercial valinomycin was 2 ng ml of extended boar semen-1. To our knowledge, this is the first report of the presence of ionophoric toxin producers in an indoor environment and the first report of valinomycin-producing strains identified as S. griseus.     

Crack initiation and arrest in a pressurized thermal shock test for a model pressure vessel made of VVER-440 reactor pressure vessel steel

A joint pressure vessel integrity research programme involving three partners is being carried out during 1990–1995. The partners are the Central Research Institute of Structural Materials “Prometey” from Russia, IVO International Ltd (IVO) from Finland, and the Technical Research Centre of Finland (VTT). The main objective of the research programme is to increase the reliability of the VVER-440 reactor pressure vessel safety analysis. This is achieved by providing material property data for the VVER-440 pressure vessel steel, and by producing experimental understanding of the crack behaviour in pressurized thermal shock loading for the validation of different fracture assessment methods. The programme is divided into four parts: pressure vessel tests, material characterization, computational fracture analyses, and evaluation of the analysis methods. The testing programme comprises tests on two model pressure vessels with artificial axial outer surface flaws. The first model vessel had circumferential weld seam at the mid-length of the vessel. A special embrittling heat treatment is applied to the vessels before tests to simulate the fracture toughness at the end-of-life condition of a real reactor pressure vessel. The sixth test on the first model led to crack initiation followed by arrest. After the testing phase, material characterization was performed. Comparison of calculated and experimental data generally led to a good correlation, although the work is being continued to resolve the discrepancies between the measured initiation and arrest properties of the material.