Gallium gradients in Cu(In,Ga)Se2 thin‐film solar cells

The gallium gradient in Cu(In,Ga)Se₂ (CIGS) layers, which forms during the two industrially relevant deposition routes, the sequential and co‐evaporation processes, plays a key role in the device performance of CIGS thin‐film modules. In this contribution, we present a comprehensive study on the formation, nature, and consequences of gallium gradients in CIGS solar cells. The formation of gallium gradients is analyzed in real time during a rapid selenization process by in situ X‐ray measurements. In addition, the gallium grading of a CIGS layer grown with an in‐line co‐evaporation process is analyzed by means of depth profiling with mass spectrometry. This gallium gradient of a real solar cell served as input data for device simulations. Depth‐dependent occurrence of lateral inhomogeneities on the µm scale in CIGS deposited by the co‐evaporation process was investigated by highly spatially resolved luminescence measurements on etched CIGS samples, which revealed a dependence of the optical bandgap, the quasi‐Fermi level splitting, transition levels, and the vertical gallium gradient. Transmission electron microscopy analyses of CIGS cross‐sections point to a difference in gallium content in the near surface region of neighboring grains. Migration barriers for a copper‐vacancy‐mediated indium and gallium diffusion in CuInSe₂ and CuGaSe₂ were calculated using density functional theory. The migration barrier for the In_Cu antisite in CuGaSe₂ is significantly lower compared with the Ga_Cu antisite in CuInSe₂, which is in accordance with the experimentally observed Ga gradients in CIGS layers grown by co‐evaporation and selenization processes. Copyright © 2014 John Wiley & Sons, Ltd.     

Global data and control in multicomputers: Operating system primitives and experimentation with a parallel branch-and-bound algorithm

The static distribution of work among tasks is not possible in many parallel applications. Therefore, it is essential to implement convenient and efficient abstractions for ‘work sharing’ on multicomputers. This paper compares the utility of two operating system facilities for the implementation of such ‘work sharing’: (1) a system for the migration of processes from heavily to less loaded processors and (2) a more general OS construct for the implementation of arbitrary distributed objects. Both were implemented as extensions to the Intel iPSC/1 operating system on a 32-node hypercube. Their experimental evaluation is based on a parallel implementation of a branch-and-bound algorithm. Two sets of results are attained. First, the necessity of the constructs for dynamic work sharing is demonstrated for applications with dynamic data domains, such as parallel branch-and-bound algorithms. This is followed by measurements that demonstrate the acceptable cost of process migration for a specific parallel branch-and-bound algorithm. These measurements are then compared with results attained with the construct for the implementation of distributed objects. Second, when using branch-and-bound to solve the Travelling Salesperson Problem (TSP), evaluation of the resulting parallel TSP program shows that some analytical and simulation results attained in past, published work may not hold.     

VIS/NIR spectroscopy for non-destructive freshness assessment of Atlantic salmon (Salmo salar L.) fillets

Visible/near-infrared spectroscopy has been evaluated for use in freshness prediction and frozen-thawed classification of farmed Atlantic salmon fillets, where fresh samples were stored as whole fish in ice. A handheld interactance probe for performing rapid measurements of single fillets and an imaging spectrometer for online analysis at an industrial speed of one fillet per second, have been used. Freshness as storage days in ice is predicted with an accuracy of 2.4 days for individual fillets, whereas frozen-thawed salmon fillets are completely separated from fresh fillets. The prediction results are comparable to previous results using the Quality Index Method with trained panelists. The region between 605 and 735 nm, which excludes interference by carotenoids and water, is appropriate for both frozen-thawed classification and freshness prediction of salmon fillets. The results indicate that the spectral changes are explained mainly by oxidation of heme proteins during the freeze–thaw cycle and during chilled storage in ice.     

Einflu? von Ethanol auf den Trocknungsverlauf und die Makrostruktur eines Einzel-Zellmaterials aus Apfelparenchym (Sorte Granny Smith)

Ethanol pre-treated apple parenchyma cells are dried in a laboratory vacuum dryer. Various ethanol contents (75 to 95% m/m) and moisture contents (2 to 15 g ethanol-water-mixture per g dry mass) of the cells were adjusted before drying and the influence of these factors on drying behaviour as well as the related structural changes are studied. The initial ethanol content was found to have a significant influence on both, drying behaviour and macrostructure of the dried material. In the case of a low initial value, the ethanol content during drying already drops at moisture contents of about 0.5–1.5 g/g. As a consequence the residual particle moisture consists mainly of water at the end of drying. In contrast to high initial ethanol contents, cells at a low initial ethanol content become strongly deformed on drying. The porosity of the dried material also decreases in the latter case, relatively large and stable aggregates are formed and the water binding capacity of the dried product has diminished on a large scale. The initial moisture content influences the drying behaviour especially on applying low initial ethanol contents. If the initial moisture content is reduced the drop of the ethanol content is shifted towards the end of the drying process. The influence of initial moisture content on macrostructure, however, was found to be small compared with the effect of the initial ethanol content.

     

Systematic survey on the prevalence of genes coding for staphylococcal enterotoxins SElM, SElO, and SElN

Staphylococcus aureus remains a leading cause of food-poisoning with substantial impact on public health. Using a multiplex polymerase chain reaction-DNA enzyme immunoassay (PCR-DEIA), we studied the presence of genes encoding staphylococcal enterotoxin-like (SEl) superantigens sem, sen, and seo, associated with the enterotoxin gene cluster (egc), in 429 clinical Staphylococcus aureus isolates. 294 (68.5%) isolates tested positive for at least one of the three SEl genes. In contrast to the fixed gene combination seg/sei also located on egc, a substantial number of isolates (n = 108) were found to bear only one or two of the genes encoding SElM, SElN, and SElO. Regarding the origin of the S. aureus isolates, a significant difference (P = 0.022) was found for the possession of seo (61.2% of blood isolates versus 42.9% of nasal strains). Also sem (not significantly) was found more common in blood isolates (52.1% versus 40.5%). The survey of the newly described SEl genes sem-seo supports the concept that most clinical S. aureus isolates harbor subsets of pyrogenic toxin superantigens. The potential contribution of seo and sem to the pathogenic potential of S. aureus has to be further evaluated.     

Deformation-mediated superstructures and cavitation of poly (l-lactide): In-situ small-angle X-ray scattering study

Cavitation and superstructure evolution of polymers during stretching play crucial roles to influence the mechanical properties of materials. In this study, we investigated deformation-mediated superstructures and cavitation of poly (l-lactide) (PLA) as well as their dependence on stretching temperatures by in-situ small-angle X-ray (SAXS) analysis coupled with mechanical testing. It is found that the cavitation and crystalline deformation are strongly influenced by stretching stress during deformation, which significantly depends on the stretching temperature. At lower stretching temperature (70 °C), the cavitation is initiated before the yielding and then stimulates the crystallite shearing. At higher stretching temperature (90 °C), however, the crystallites shear firstly and then crystalline deformation promotes the formation of cavities orientated along the stretching direction. High stretching temperature benefits the formation of relatively perfect crystals with high orientation. The results provide the basic knowledge of how to adjust the mechanical properties of polymer materials by controlling their superstructure in the deformation process.     

A Synthetic Approach to Star‐Like Polymers of Styrene and Butyl Acrylate by Linking Reactions using Functionalized Methacrylates

Summary: Coupling reactions between terminal functionalized polymer chains were chosen for the synthesis of star‐like polymers consisting of polystyrene and polystyrene‐block‐poly[styrene‐co‐(butyl acrylate)] arms. For the preparation of terminal functionalized polymer chains a side reaction of the 2,2,6,6‐tetramethylpiperidine‐N‐oxyl (TEMPO) mediated free radical polymerization of methacrylates could be used successfully to convert TEMPO terminated polymers into end functionalized polymers. The number of functionalized monomer units attached to the polymer chain is directly related to the TEMPO concentration during this reaction. Different polystyrenes and polystyrene‐block‐poly[styrene‐co‐(butyl acrylate)] block copolymers were functionalized with a variable number of epoxide and alcohol groups at the chain end. For the determination of the optimal reaction parameters for the coupling reactions between these polymer chains, epoxy functionalized polystyrenes were converted with hydroxy functionalized polystyrenes under basic and acidic conditions. By activation with sodium hydride or boron trifluoride star‐like polymers were synthesized under mild conditions. The transfer of the reaction conditions to coupling reactions between end functionalized polystyrene‐block‐poly[styrene‐co‐(butyl acrylate)] copolymers showed that star‐like polymers with more than 12 arms were formed using boron trifluoride as activating agent.

     

Bivalent EGFR-Targeting DARPin-MMAE Conjugates

Epidermal growth factor receptor (EGFR) is a validated tumor marker overexpressed in various cancers such as squamous cell carcinoma (SSC) of the head and neck and gliomas. We constructed protein-drug conjugates based on the anti-EGFR Designed Ankyrin Repeat Protein (DARPin) E01, and compared the bivalent DARPin dimer (DD1) and a DARPin-Fc (DFc) to the monomeric DARPin (DM) and the antibody derived scFv425-Fc (scFvFc) in cell culture and a mouse model. The modular conjugation system, which was successfully applied for the preparation of protein-drug and -dye conjugates, uses bio-orthogonal protein-aldehyde generation by the formylglycine-generating enzyme (FGE). The generated carbonyl moiety is addressed by a bifunctional linker with a pyrazolone for a tandem Knoevenagel reaction and an azide for strain-promoted azide-alkyne cycloaddition (SPAAC). The latter reaction with a PEGylated linker containing a dibenzocyclooctyne (DBCO) for SPAAC and monomethyl auristatin E (MMAE) as the toxin provided the stable conjugates DD1-MMAE (drug-antibody ratio, DAR = 2.0) and DFc-MMAE (DAR = 4.0) with sub-nanomolar cytotoxicity against the human squamous carcinoma derived A431 cells. In vivo imaging of Alexa Fluor 647-dye conjugates in A431-xenografted mice bearing subcutaneous tumors as the SCC model revealed unspecific binding of bivalent DARPins to the ubiquitously expressed EGFR. Tumor-targeting was verified 6 h post-injection solely for DD1 and scFvFc. The total of four administrations of 6.5 mg/kg DD1-MMAE or DFc-MMAE twice weekly did not cause any sequela in mice. MMAE conjugates showed no significant anti-tumor efficacy in vivo, but a trend towards increased necrotic areas (p = 0.2213) was observed for the DD1-MMAE (n = 5).