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排序方式: 共有410条查询结果,搜索用时 15 毫秒
71.
72.
Hosomi N Kawamura-Konishi Y Kawano R Fujii I Suzuki H 《Journal of Bioscience and Bioengineering》2005,99(3):222-229
Monoclonal antibody 2D7 generated against a transition-state analog N-methyl mesoporphyrin catalyzes a reaction for insertion of a cupric ion into mesoporphyrin. To investigate amino acid residues responsible for the catalytic activity, site-directed mutagenesis of the amino acid residues in the third complementarity determining region of the heavy chain (CDRH3) was performed on the antigen-binding fragment (Fab) of the antibody. Recombinant Fab mutants, in which Arg95 is replaced with Ala (R95A), Asp96 with Asn (D96N) and Met97 with Gly (M97G), were examined in terms of the catalytic efficiency of the reaction (k/K(S)) and the dissociation constant for N-methyl mesoporphyrin binding (K(d)) and these values were compared with those of the wild type. The k/K(S) values of the R95A and D96N mutants were 0.96% and 1.0% of that of the wild type, respectively, whereas the M97G mutant had no detectable catalytic activity. The K(d) values of the R95A and D96N mutants were 165 and 69 times that of the wild type, respectively, while that of the M97G mutant was similar to that of the wild type. The relationship between the k/K(S) and 1/K(d) values in the wild type and the R95A and D96N mutants suggests that Arg95 and Asp96 are responsible for stabilizing the transition-state in the catalytic reaction. The results of the M97G mutant allow us to propose that Met97 plays an important role in the catalytic activity probably due to a subtle and specific conformation of the antibody. 相似文献
73.
In the cement industry, many optimization systems have been developed and are currently in operation to reduce total production and delivery costs or to optimize the efficiency of equipment investments. This paper deals with some of these optimization systems, which were designed with mathematical programming. Some effective usage of sensitivity analysis and a systems integration approach are also introduced 相似文献
74.
K Kugiyama H Doi T Motoyama H Soejima K Misumi H Kawano O Nakagawa M Yoshimura H Ogawa T Matsumura S Sugiyama T Nakano K Nakajima H Yasue 《Canadian Metallurgical Quarterly》1998,97(25):2519-2526
BACKGROUND: It remains undetermined whether triglyceride-rich lipoproteins are an independent risk factor for atherosclerosis. METHODS AND RESULTS: The correlation of responses of coronary arterial diameter (quantitative coronary angiography) and coronary blood flow (intracoronary flow wire technique) to intracoronary infusion of acetylcholine (10 and 50 microg/min) with coronary risk factors including remnant lipoprotein levels was statistically analyzed in 106 consecutive subjects with normal coronary angiograms. Remnant lipoproteins were isolated from fasting blood with an immunoaffinity mixed gel containing anti-apolipoprotein (apo) A-1 and anti-apoB-100 monoclonal antibodies. In multivariate stepwise regression analysis, remnant lipoprotein levels had the most significant correlation with abnormal epicardial coronary vasomotor responses to acetylcholine infusion, reflected by impaired dilation or constriction of the epicardial coronary arteries, and the levels also had an inverse and independent correlation with the coronary blood flow increase in response to acetylcholine. In a subgroup of 53 consecutive subjects, constrictor responses of epicardial coronary diameters to intracoronary infusion of NG-monomethyl-L-arginine (50 micromol/min for 4 minutes) at baseline, reflecting the presence of coronary nitric oxide bioactivity, had an inverse and independent correlation with remnant lipoprotein levels by use of multivariate analysis. CONCLUSIONS: Remnant lipoprotein levels were independently associated with abnormal endothelium-dependent vasomotor function in large and resistance coronary arteries in humans, indicating that remnant lipoproteins may impair endothelial vasomotor function in human coronary arteries. The decrease in coronary nitric oxide bioactivity may be responsible in part for the inhibitory effects of remnant lipoproteins. 相似文献
75.
电子纸(EPD)技术有一些瑕疵.缺乏市场成熟性。电子纸用户是少数,围绕EPD的生态体系和下层基础构建还在发展中,比如LCD显示器拥有众多的标准驱动电路支持,但可以设想,EPD的瑕疵会随着市场的发展而淡化。EPD的其它缺陷还包括有电压要求:即高压驱动(〉10V),尽管驱动电流非曾低。随着时间推移EPD比其它显示媒介缩短了响应时间。当然,尽管存在这些缺点,但都会在:降来得到改正,EPD为各种创新应用提供了独到的方案。分段式EPD凭据其超薄、不规謦性、柔韧性能,可为各种类型的显示器提供高对比显示,通过运用低功耗电子系统来匹配低功耗图形显示,设计师可以实现产品便携和长寿电池的双重性能。 相似文献
76.
Kawano M. Takahashi N. Kurita Y. Soejima K. Komuro M. Matsui S. 《Electron Devices, IEEE Transactions on》2008,55(7):1614-1620
A 3-D packaging technology is developed for stacked dynamic random access memory (DRAM) with through-silicon vias (TSVs). Eight different dry etchers were evaluated for deep Si etching. Highly doped poly-Si TSVs were used for vertical traces inside silicon and interconnection between DRAM chips to realize a DRAM-compatible process. Through optimization of process conditions and layout design, a fast poly-Si filling has been obtained. The entire packaging was carried out at the wafer level by using smart chip connection with feedthrough interposer (FTI) technology. A new bump and wiring structure for the FTI has also been developed for fine-pitch and low-cost bonding. Normal operation during DRAM read/write was confirmed on a 512-Mb DRAM with TSVs, with an I/F chip as a memory controller. Simulation and measurement of the transfer function of the FTI wiring showed a 3-Gb/s/pin data transfer capability. 相似文献
77.
Terahertz (THz) imaging is expected to become powerful tools for non-destructive inspections. To ensure the practical use of THz non-destructive monitoring, versatile THz imagers with adjustable designs that can eliminate the complexities and the bulkiness of the device are urgently required. Herein, a self-aligned filtration process for a 2D, free-standing carbon nanotube film array and its application to a THz video camera patch are reported. The presented techniques enable a) to freely design the camera size, sensor array pattern, and suspended shape according to its applications, b) to cut the camera patch into desired shapes, and c) to attach them to the objects that are intended to be measured. Real-time, non-destructive monitoring of various infrastructures is demonstrated. These results indicate that it can function regardless of restrictions, such as the shapes and locations of the measurement samples, thus providing a strong possibility for use in future non-destructive sensor networks. 相似文献
78.
Atsushi Kawano Kansuke Fukui Yuji Matsumoto Atsushi Terada Akihiro Tominaga Nozomi Nikaido Takashi Tonozuka Kazuhide Totani Nozomu Yasutake 《Journal of Applied Glycoscience》2020,67(2):41
According to whole-genome sequencing, Aspergillus niger produces multiple enzymes of glycoside hydrolases (GH) 31. Here we focus on a GH31 α-glucosidase, AgdB, from A. niger . AgdB has also previously been reported as being expressed in the yeast species, Pichia pastoris ; while the recombinant enzyme (rAgdB) has been shown to catalyze tranglycosylation via a complex mechanism. We constructed an expression system for A. niger AgdB using Aspergillus nidulans . To better elucidate the complicated mechanism employed by AgdB for transglucosylation, we also established a method to quantify glucosidic linkages in the transglucosylation products using 2D NMR spectroscopy. Results from the enzyme activity analysis indicated that the optimum temperature was 65 °C and optimum pH range was 6.0–7.0. Further, the NMR results showed that when maltose or maltopentaose served as the substrate, α-1,2-, α-1,3-, and small amount of α-1,1-β-linked oligosaccharides are present throughout the transglucosylation products of AgdB. These results suggest that AgdB is an α-glucosidase that serves as a transglucosylase capable of effectively producing oligosaccharides with α-1,2-, α-1,3-glucosidic linkages. 相似文献
79.
H Ohtake H Urayama S Katada M Kawasuji Y Watanabe M Kawano 《Canadian Metallurgical Quarterly》1998,40(4):329-331
Those who work in the area of vocabularies, like all researchers, bring some assumptions to that work. Such assumptions include both cultural notions and beliefs specific to particular social worlds within the broader society. In this article, assumptions and beliefs expressed by participants during the conference on "Implementation of Nursing Vocabularies in Computer-based Systems" are summarized. Questions are raised concerning the relationship between cultural notions and beliefs and nursing vocabularies. 相似文献
80.
Seiichi Suda Koichi Kawahara Mitsunobu Kawano Hiroyuki Yoshida Toru Inagaki 《Journal of the American Ceramic Society》2007,90(4):1094-1100
Matrix-type nickel oxide (NiO)/samarium-doped ceria (SDC) composite particles, in which NiO and SDC nano-particles were homogeneously dispersed, were synthesized by spray pyrolysis (SP) for an anode precursor of intermediate-temperature solid oxide fuel cells (IT-SOFCs). SP of an aqueous solution containing Ni, Ce, and Sm salts resulted in capsule-type composite particles that had NiO enveloped with SDC. The capsule-type composite particles actually prevent Ni aggregation between particles, but they cannot have a large contact area between nickel (Ni) and SDC. A matrix-type composite particle is expected to have a large contact area because the matrix-type composite is comprised of nanometer-sized Ni and SDC particles. An adequate addition of ethylene glycol successfully resulted in matrix-type NiO/SDC composite particles. The matrix-type composite particles also showed higher anode performance than the capsule-type composite particles in these experiments and they were effective as precursors of high-performance IT-SOFC anodes. 相似文献